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Hydrolysable tannin-based diet rich in gallotannins has a minimal impact on pig performance but significantly reduces salivary and bulbourethral gland size

  • G. Bee (a1), P. Silacci (a1), S. Ampuero-Kragten (a1), M. Čandek-Potokar (a2), A. L. Wealleans (a1) (a3), J. Litten-Brown (a3), J.-P. Salminen (a4) and I. Mueller-Harvey (a3)...
Abstract

Tannins have long been considered ‘anti-nutritional’ factors in monogastric nutrition, shown to reduce feed intake and palatability. However, recent studies revealed that compared with condensed tannins, hydrolysable tannins (HT) appear to have far less impact on growth performance, but may be inhibitory to the total activity of caecal bacteria. This in turn could reduce microbial synthesis of skatole and indole in the hindgut of entire male pigs (EM). Thus, the objective of this study was to determine the impact of a group of dietary HT on growth performance, carcass traits and boar taint compounds of group housed EM. For the study, 36 Swiss Large White boars were assigned within litter to three treatment groups. Boars were offered ad libitum one of three finisher diets supplemented with 0 (C), 15 (T15) or 30 g/kg (T30) of HT from day 105 to 165 of age. Growth performance, carcass characteristics, boar taint compounds in the adipose tissue and cytochrome P450 (CYP) isoenzymes CYP2E1, CYP1A2 and CYP2A19 gene expression in the liver was assessed. Compared with C, feed efficiency but not daily gain and daily feed intake was lower (P<0.05) in T15 and T30 boars. Except for the percentage carcass weight loss during cooling, which tended (P<0.10) to be greater in T30 than C and T15, carcass characteristics were not affected by the diets. In line with the numerically lower androstenone level, bulbourethral and salivary glands of T30 boars were lighter (P<0.05) than of T15 with intermediate values for C. Indole level was lower (P<0.05) in the adipose tissue of T30 than C pigs with intermediate levels in T15. Skatole levels tended (P<0.10) to be lower in T30 and C than T15 pigs. Hepatic gene expression of CYP isoenzymes did not differ between-treatment groups, but was negatively correlated (P<0.05) with androstenone (CYP2E1 and CYP1A2), skatole (CYP2E1, CYP2A) and indole (CYP2A) level. In line with the numerically highest androstenone and skatole concentrations, boar taint odour but not flavour was detected by the panellists in loins from T15 compared with loins from C and T30 boars. These results provide evidence that HT affected metabolism of indolic compounds and androstenone and that they affected the development of accessory sex glands. However, the effects were too small to be detected by sensory evaluation.

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Copyright
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
E-mail: giuseppe.bee@agroscope.admin.ch
References
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Abarikwu SO, Akiri OF, Durojaiye MA and Alabi AF 2014. Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. The Journal of Steroid Biochemistry and Molecular Biology 143, 4960.
Aluwe Md, Langendries KCM, Bekaert KM, Tuyttens FAM, De Brabander DL, De Smet S and Millet S 2013. Effect of surgical castration, immunocastration and chicory-diet on the meat quality and palatability of boars. Meat Science 94, 402407.
Ampuero Kragten S, Verkuylen B, Dahlmans H, Hortos M, Garcia-Regueiro JA, Dahl E, Andresen O, Feitsma H, Mathur PK and Harlizius B 2011. Inter-laboratory comparison of methods to measure androstenone in pork fat. Animal 5, 16341642.
Antongiovanni M, Minieri S and Petacchi F 2007. Effect of tannin supplementation on nitrogen digestibility and retention in growing pigs. Italian Journal of Animal Science 6 (suppl. 1), 245247.
Bee G, Chevillon P and Bonneau M 2015. Entire male pig production in Europe. Animal Production Science 55, 13471359.
Bee G, Guex G and Herzog W 2004. Free-range rearing of pigs during the winter: adaptations in muscle fiber characteristics and effects on adipose tissue composition and meat quality traits. Journal of Animal Science 82, 12061218.
Bee G, Jacot S, Guex G and Biolley C 2008. Effects of two supplementation levels of linseed combined with CLA or tallow on meat quality traits and fatty acid profile of adipose and different muscle tissues in slaughter pigs. Animal 2, 800811.
Bi J and O’Mahony M 2007. Updated and extended table for testing the significance of the R-index. Journal of Sensory Studies 22, 713720.
Biagia G, Cipollini I, Paulicks BR and Roth FX 2010. Effect of tannins on growth performance and intestinal ecosystem in weaned piglets. Archives of Animal Nutrition 64, 121135.
Bonneau M and Chevillon P 2012. Acceptability of entire male pork with various levels of androstenone and skatole by consumers according to their sensitivity to androstenone. Meat Science 90, 330337.
Čandek-Potokar M, Škrlep M, Batorek Lukač N, Zamaratskaia G, Prevolnik Povše M, Velikonja Bolta Š, Kubale V and Bee G 2015. Hydrolysable tannin fed to entire male pigs affects intestinal production, tissue deposition and hepatic clearance of skatole. The Veterinary Journal 204, 162167.
Cantos E, Espín JC, López-Bote C, de la Hoz L, Ordóñez JA and Tomás-Barberán FA 2003. Phenolic compounds and fatty acids from acorns (Quercus spp.), the main dietary constituent of free-ranged Iberian pigs. Journal of Agricultural and Food Chemistry 51, 62486255.
Cousins BW, Tanksley TD, Knabe DA and Zebrowska T 1981. Nutrient digestibility and performance of pigs fed sorghums varying in tannin concentration. Journal of Animal Science 53, 15241537.
Cava R, Ventanas J, Florencio Tejeda J, Ruiz J and Antequera T 2000. Effect of free-range rearing and α-tocopherol and copper supplementation on fatty acid profiles and susceptibility to lipid oxidation of fresh meat from Iberian pigs. Food Chemistry 68, 5159.
Dias TR, Alves MG, Oliveira PF and Silva BM 2014. Natural products as modulators of spermatogenesis: the search for a male contraceptive. Current Molecular Pharmacology 7, 154166.
Doran E, Whittington FW, Wood JD and McGivan JD 2002. Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes. Chemico-Biological Interactions 140, 8192.
Engström MT, Pälijärvi M and Salminen J-P 2015. Rapid fingerprint analysis of plant extracts for ellagitannins, gallic acid, and quinic acid derivatives and quercetin-, kaempferol- and myricetin-based flavonol glycosides by UPLC-QqQ-MS/MS. Journal of Agricultural and Food Chemistry 63, 40684079.
Espín JC, González-Barrio R, Cerdá B, López-Bote C, Rey AI and Tomás-Barberán FA 2007. Iberian pig as a model to clarify obscure points in the bioavailability and metabolism of ellagitannins in humans. Journal of Agricultural and Food Chemistry 55, 1047610485.
Ferguson NS, Gous RM and Iji PA 2003. Determining the source of anti-nutritional factor(s) found in two species of lupin (L. albus and L. angustifolius) fed to growing pigs. Livestock Production Science 84, 8391.
Hagerman AE, Robbins CT, Yohan W, Wilson TC and McArthur C 1992. Tannin chemistry in relation to digestion. Journal of Range Management 45, 5762.
Hansen-Møller J 1994. Rapid high-performance liquid chromatographic method for simultaneous determination of androstenone, skatole and indole in back fat from pigs. Journal of Chromatography B: Biomedical Sciences and Applications 661, 219230.
Johnson MTJ, Ives AR, Ahern J and Salminen JP 2014. Macroevolution of plant defenses against herbivores in the evening primroses. New Phytologist 203, 267279.
Knarreborg A, Beck J, Jensen MT, Laue A, Agergaard N and Jensen BB 2002. Effect of non-starch polysaccharides on production and absorption of indolic compounds in entire male pigs. Animal Science 74, 445453.
Koller M 2013. Robust estimation of linear mixed models. PhD thesis, ETH Zurich, Zurich, Switzerland.
Koller M 2014. robustlmm: Robust Linear Mixed Effects Models. R package version 1.4-2. Retrieved on 10 March 2015 from http://cran.r-project.org/web/packages/robustlmm/vignettes/rlmer.pdf.
Korobkova EA 2015. Effect of natural polyphenols on CYP metabolism: implications for diseases. Chemical Research in Toxicology 28, 13591390.
Mariscal-Landín G, Lebreton Y and Sève B 2002. Apparent and standardised true ileal digestibility of protein and amino acids from faba bean, lupin and pea, provided as whole seeds, dehulled or extruded in pig diets. Animal Feed Science and Technology 97, 183198.
Meier-Dinkel L, Gertheiss J, Müller S, Wesoly R and Mörlein D 2015. Evaluating the performance of sensory quality control: the case of boar taint. Meat Science 100, 7384.
Moilanen J, Sinkkonen J and Salminen JP 2013. Characterization of bioactive plant ellagitannins by chromatographic, spectroscopic and mass spectrometric methods. Chemoecology 23, 165179.
Mueller-Harvey I 2006. Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture 86, 20102037.
Nagata Y and Takeuchi N 2003. Measurement of odor threshold by triangle odor bag method. Odor Measurement Review 118, 118127.
O’Mahony M 1988. Sensory difference and preference testing: the use of signal detection measures. In Applied Sensory Analysis of Food (ed. H Moskowitz), p. 148, CRC Press, Boca Raton, FL.
O’Mahony M 1992. Understanding discrimination tests: a user-friendly treatment of response bias, rating and ranking R-index tests and their relationship to signal detection. Journal of Sensory Studies 7, 147.
Pauly C, Spring P, Doherty JV, Ampuero Kragten S and Bee G 2008. Performances, meat quality and boar taint of castrates and entire male pigs fed a standard and a raw potato starch-enriched diet. Animal 2, 17071715.
Rasmussen MK, Zamaratskaia G and Ekstrand B 2011. In vivo effect of dried chicory root (Cichorium intybus L.) on xenobiotica metabolising cytochrome P450 enzymes in porcine liver. Toxicology Letters 200, 8891.
Stukelj M, Valencak Z, Krsnik M and Svete AN 2010. The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Acta Veterinaria Scandinavica 52, 19.
Wesoly R and Weiler U 2012. Nutritional influences on skatole formation and skatole metabolism in the pig. Animals 2, 221242.
Wiercinska P, Lou Y and Squires EJ 2012. The roles of different porcine cytochrome P450 enzymes and cytochrome b5A in skatole metabolism. Animal 6, 834845.
Xue J and Dial GD 1997. Raising intact male pigs for meat: detecting and preventing boar taint. Swine Health and Production 5, 151158.
Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S and Madden T 2012. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 13, 134.
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