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Mitochondrial abundance and function in skeletal muscle and liver from Simmental beef cattle divergent for residual feed intake

Published online by Cambridge University Press:  16 March 2020

C. McKenna Open the ORCID record for C. McKenna [Opens in a new window] ,
R. K. Porter ,
C. Fitzsimons ,
S. M. Waters ,
M. McGee  and
D. A. Kenny
C. McKenna
Affiliation:
Animal and Bioscience Department, Animal and Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, County MeathC15 PW93, Ireland School of Biochemistry & Immunology, Trinity College Dublin, Dublin 2D02 R590, Ireland
R. K. Porter
Affiliation:
School of Biochemistry & Immunology, Trinity College Dublin, Dublin 2D02 R590, Ireland
C. Fitzsimons
Affiliation:
Animal and Bioscience Department, Animal and Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, County MeathC15 PW93, Ireland
S. M. Waters
Affiliation:
Animal and Bioscience Department, Animal and Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, County MeathC15 PW93, Ireland
M. McGee
Affiliation:
Animal and Bioscience Department, Animal and Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, County MeathC15 PW93, Ireland
D. A. Kenny*
Affiliation:
Animal and Bioscience Department, Animal and Grassland Research and Innovation Centre, Teagasc Grange, Dunsany, County MeathC15 PW93, Ireland
*
†E-mail: david.kenny@teagasc.ie
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Abstract

Cellular mitochondrial function has been suggested to contribute to variation in feed efficiency (FE) among animals. The objective of this study was to determine mitochondrial abundance and activities of various mitochondrial respiratory chain complexes (complex I (CI) to complex IV (CIV)) in liver and muscle tissue from beef cattle phenotypically divergent for residual feed intake (RFI), a measure of FE. Individual DM intake (DMI) and growth were measured in purebred Simmental heifers (n = 24) and bulls (n = 28) with an initial mean BW (SD) of 372 kg (39.6) and 387 kg (50.6), respectively. All animals were offered concentrates ad libitum and 3 kg of grass silage daily, and feed intake was recorded for 70 days. Residuals of the regression of DMI on average daily gain (ADG), mid-test BW0.75 and backfat (BF), using all animals, were used to compute individual RFI coefficients. Animals were ranked within sex, by RFI into high (inefficient; top third of the population), medium (middle third of population) and low (efficient; bottom third of the population) terciles. Statistical analysis was carried out using the MIXED procedure of SAS v 9.3. Overall mean ADG (SD) and daily DMI (SD) for heifers were 1.2 (0.4) and 9.1 (0.5) kg, respectively, and for bulls were 1.8 (0.3) and 9.5 (1.02) kg, respectively. Heifers and bulls ranked as high RFI consumed 10% and 15% more (P < 0.05), respectively, than their low RFI counterparts. There was no effect of RFI on mitochondrial abundance in either liver or muscle (P > 0.05). An RFI × sex interaction was apparent for CI activity in muscle. High RFI animals had an increased activity (P < 0.05) of CIV in liver tissue compared to their low RFI counterparts; however, the relevance of that observation is not clear. Our data provide no clear evidence that cellular mitochondrial function within either skeletal muscle or hepatic tissue has an appreciable contributory role to overall variation in FE among beef cattle.

Keywords

feed efficiencygenderoxidative phosphorylationcitrate synthasebovine
Type
Research Article
Information
animal , Volume 14 , Issue 8 , August 2020 , pp. 1710 - 1717
Copyright
© The Animal Consortium 2020

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