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Validation of Brix refractometer to estimate colostrum immunoglobulin G content and composition in the sow

Published online by Cambridge University Press:  06 May 2016

S. M. K. Hasan ,
S. Junnikkala ,
A. Valros ,
O. Peltoniemi  and
C. Oliviero
S. M. K. Hasan*
Affiliation:
Department of Production Animal Medicine, Faculty of Veterinary Medicine, Agnes Sjöbergin katu 2, 00014University of Helsinki, Finland
S. Junnikkala
Affiliation:
Department of Veterinary Biosciences, Faculty of Veterinary Medicine, Agnes Sjöbergin katu 2, 00014University of Helsinki, Finland
A. Valros
Affiliation:
Department of Production Animal Medicine, Faculty of Veterinary Medicine, Agnes Sjöbergin katu 2, 00014University of Helsinki, Finland
O. Peltoniemi
Affiliation:
Department of Production Animal Medicine, Faculty of Veterinary Medicine, Agnes Sjöbergin katu 2, 00014University of Helsinki, Finland
C. Oliviero
Affiliation:
Department of Production Animal Medicine, Faculty of Veterinary Medicine, Agnes Sjöbergin katu 2, 00014University of Helsinki, Finland
*
†E-mail: shah.hasan@helsinki.fi
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Abstract

Colostrum is an essential source of immunoglobulin G (IgG) for neonate piglets. However, colostrum IgG content and nutritional composition can vary considerably among sows due to age, parity, feeding regime and immunological background. Currently, there is no practical way to obtain information about colostrum IgG concentration at herd level. We evaluated sows’ colostrum IgG content on-farm using a Brix refractometer and its performance was compared with that of an IgG ELISA. In addition, nutritional compositions of the colostrum samples were analyzed using Fourier transform IR spectroscopy. Colostrum samples (5 to 6 ml) (n=153) were obtained within 0 to 3 h of farrowing. However, to obtain a 24 h IgG profile for 11 sows, colostrum samples were collected at 0, 2, 4, 6, 8, 10, 16 and 24 h after farrowing. A 0.3 ml of freshly drawn colostrum sample was used for the on-farm measurement of Brix percentages using a digital refractometer shortly after collection. The remaining fractions of the samples were frozen and submitted to laboratory analysis for total IgG, using a commercially available pig IgG ELISA kit. For nutritional composition analysis, a 35 ml colostrum sample (n=34) was obtained immediately after birth of first piglet from the first three pairs of frontal teats. Colostrum concentrations of IgG averaged 52.03±30.70 mg/ml (mean±SEM) at 0 to 3 h after farrowing. Concentration of IgG decreased on average by 50% during the 1st day of lactation (P<0.01). Sow parity did not influence colostrum concentrations of IgG. Differences in colostrum composition were recorded between two herds and among the parity groups (P<0.05). The Brix refractometer measurement of colostrum and the corresponding log transformed IgG measurements from the ELISA were moderately correlated (r=0.63, P<0.001, n=153). Based on the classification we suggest here, low levels of IgG (14.5±1.8 mg/ml) were recorded for colostrum samples with Brix readings below 20%. Borderline colostrum IgG content (43.8±2.3 mg/ml) had Brix readings of 20% to 24%, adequate colostrum IgG content (50.7±2.1 mg/ml) had Brix % readings of 25% to 29% and very good IgG colostrum content (78.6±8.4 mg/ml) had Brix readings >30%. Colostrum IgG concentration is highly variable among sows, Brix measurement of a sows’ fresh colostrum is an inexpensive, rapid and satisfactorily accurate method of estimating IgG concentration, providing indication of differentiation between good and poor IgG content of colostrum.

Keywords

colostrumsowpigletBrix refractometerimmunoglobulin G
Type
Research Article
Information
animal , Volume 10 , Issue 10 , October 2016 , pp. 1728 - 1733
Copyright
© The Animal Consortium 2016 

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References

Bielmann, V, Gillan, J, Perkins, N, Skidmore, A, Godden, S and Leslie, K 2010. An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle. Journal of Dairy Science 93, 3713–3721.Google Scholar
Cash, R 1999. Colostral quality determined by refractometry. Equine Veterinary Education 11, 36–38.Google Scholar
Chigerwe, M, Tyler, JW, Middleton, JR, Spain, JN, Dill, JS and Steevens, BJ 2008. Comparison of four methods to assess colostral IgG concentration in dairy cows. Journal of the American Veterinary Medical Association 233, 761–766.CrossRefGoogle ScholarPubMed
Decaluwé, R, Maes, D, Cools, A, Wuyts, B, De Smet, S, Marescau, B, De Deyn, PP and Janssens, G 2014a. Effect of peripartal feeding strategy on colostrum yield and composition in sows. Journal of Animal Science 92, 3557–3567.CrossRefGoogle ScholarPubMed
Decaluwé, R, Maes, D, Declerck, I, Cools, A, Wuyts, B, De Smet, S and Janssens, G 2013. Changes in back fat thickness during late gestation predict colostrum yield in sows. Animal 7, 1999–2007.CrossRefGoogle Scholar
Decaluwé, R, Maes, D, Wuyts, B, Cools, A, Piepers, S and Janssens, G 2014b. Piglets’ colostrum intake associates with daily weight gain and survival until weaning. Livestock Science 162, 185–192.Google Scholar
Devillers, N, Farmer, C, Le Dividich, J and Prunier, A 2007. Variability of colostrum yield and colostrum intake in pigs. Animal 1, 1033–1041.Google Scholar
Devillers, N, Le Dividich, J and Prunier, A 2011. Influence of colostrum intake on piglet survival and immunity. Animal 5, 1605–1612.Google Scholar
Dividich, J, Rooke, J and Herpin, P 2005. Nutritional and immunological importance of colostrum for the new-born pig. The Journal of Agricultural Science 143, 469–485.Google Scholar
Farmer, C, Charagu, P and Palin, M 2007. Influence of genotype on metabolic variables, colostrum and milk composition of primiparous sows. Canadian Journal of Animal Science 87, 511–515.Google Scholar
Farmer, C and Quesnel, H 2009. Nutritional, hormonal, and environmental effects on colostrum in sows. Journal of Animal Science 87, 56–64.CrossRefGoogle ScholarPubMed
Foisnet, A, Farmer, C, David, C and Quesnel, H 2010. Relationships between colostrum production by primiparous sows and sow physiology around parturition. Journal of Animal Science 88, 1672–1683.Google Scholar
Harker, DB 1978. A simple estimation of the immunoglobulin content of ewe colostrum. The Veterinary Record 103, 8–9.Google Scholar
Hurley, W 2015. Composition of sow colostrum and milk. In The gestating and lactating sow (ed. C Farmer), pp. 193–229. Wageningen Academic Publishers, Wageningen, The Netherlands.Google Scholar
Kielland, C, Rootwelt, V, Reksen, O and Framstad, T 2015. The association between immunoglobulin G in sow colostrum and piglet plasma. Journal of Animal Science 93, 4453–4462.Google Scholar
Klobasa, F and Butler, JE 1987. Absolute and relative concentrations of immunoglobulins G, M, and A, and albumin in the lacteal secretion of sows of different lactation numbers. American Journal of Veterinary Research 48, 176–182.Google Scholar
Oliviero, C 2013. Management to improve neonate piglet survival. In Control of pig reproduction IX (ed. H Rodriqez-Martinez, N Soede and W Flowers), pp. 203–210. Contex, Olsztyn, Poland.Google Scholar
Quesnel, H 2011. Colostrum production by sows: variability of colostrum yield and immunoglobulin G concentrations. Animal 5, 1546–1553.Google Scholar
Quesnel, H, Farmar, C and Theil, PK 2015. Colostrum and milk production. In The gestating and lactating sow (ed. C Farmer), pp. 173–192. Wageningen Academic Publishers, Wageningen, The Netherlands.Google Scholar
Quigley, J, Lago, A, Chapman, C, Erickson, P and Polo, J 2013. Evaluation of the Brix refractometer to estimate immunoglobulin G concentration in bovine colostrum. Journal of Dairy Science 96, 1148–1155.Google Scholar
Rooke, J and Bland, I 2002. The acquisition of passive immunity in the new-born piglet. Livestock Production Science 78, 13–23.CrossRefGoogle Scholar
Salmon, H, Berri, M, Gerdts, V and Meurens, F 2009. Humoral and cellular factors of maternal immunity in swine. Developmental & Comparative Immunology 33, 384–393.CrossRefGoogle ScholarPubMed
Vallet, J, Miles, J and Rempel, L 2013. A simple novel measure of passive transfer of maternal immunoglobulin is predictive of preweaning mortality in piglets. The Veterinary Journal 195, 91–97.Google Scholar