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Absorption and metabolism of bioactive molecules after oral consumption of cooked edible heads of Cynara scolymus L. (cultivar Violetto di Provenza) in human subjects: a pilot study

  • E. Azzini (a1), R. Bugianesi (a1), F. Romano (a1), D. Di Venere (a2), S. Miccadei (a3), A. Durazzo (a1), M. S. Foddai (a1), G. Catasta (a1), V. Linsalata (a2) and G. Maiani (a1)...

Abstract

The current growing interest for natural antioxidants has led to a renewed scientific attention for artichoke, due not only to its nutritional value, but, overall, to its polyphenolic content, showing strong antioxidant properties. The major constituents of artichoke extracts are hydroxycinnamic acids such as chlorogenic acid, dicaffeoylquinic acids caffeic acid and ferulic acid, and flavonoids such as luteolin and apigenin glycosides. Invitro studies, using cultured rat hepatocytes, have shown its hepatoprotective functions and in vivo studies have shown the inhibition of cholesterol biosynthesis in human subjects. Several studies have shown the effect on animal models of artichoke extracts, while information on human bioavailability and metabolism of hydroxycinnamates derivatives is still lacking. Results showed a plasma maximum concentration of 6·4 (sd 1.8) ng/ml for chlorogenic acid after 1 h and its disappearance within 2 h (P < 0·05). Peak plasma concentrations of 19·5 (sd6·9) ng/ml for total caffeic acid were reached within 1 h, while ferulic acid plasma concentrations showed a biphasic profile with 6·4 (sd1·5) ng/ml and 8·4 (sd4·6) ng/ml within 1 h and after 8 h respectively. We observed a significant increase of dihydrocaffeic acid and dihydroferulic acid total levels after 8 h (P < 0·05). No circulating plasma levels of luteolin and apigenin were present. Our study confirms the bioavailability of metabolites of hydroxycinnamic acids after ingestion of cooked edible Cynara scolymus L. (cultivar Violetto di Provenza).

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      Absorption and metabolism of bioactive molecules after oral consumption of cooked edible heads of Cynara scolymus L. (cultivar Violetto di Provenza) in human subjects: a pilot study
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Corresponding author

*Corresponding author: Dr. Giuseppe Maiani, fax +39 06 51494550, email maiani@inran.it

References

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Abu Amsha Caccetta, RA, Croft, KD, Puddey, IB, Proudfoot, JM & Beilin, LJ (1996) Phenolic content of various beverages determines the extent of inhibition of human serum and low density lipoprotein oxidation in vitro: identification and mechanism of action of some cinnamic acid derivatives from red wine. Clin Sci 91, 449458.
Adzet, T, Camarasa, J & Laguna, JC (1987) Hepatoprotective activity of polyphenolic compounds from Cynara Scolymus against CCl4 toxicity in isolated rat hepatocytes. J Nat Prod 50, 612617.
Andreasen, MF, Kroon, PA, Williamson, G & Garcia-Conesa, MT (2001) Esterase activity able to hydrolyze dietary antioxidant hydroxycinnamates is distributed along the intestine of mammals. J Agric Food Chem 49, 56795684.
Azuma, K, Ippoushi, K, Nakayama, M, Ito, H, Higashio, H & Terao, J (2000) Absorption of chlorogenic acid and caffeic acid in rat after oral administration. J Agric Food Chem 48, 54965500.
Booth, AN, Emerson, OH, Jones, FT & Deeds, F (1957) Urinary metabolites of caffeic and chlorogenic acids. J Biol Chem 229, 5159.
Brenes, M, García, A, Dobarganes, MC, Velasco, J & Romero, C (2002) Influence of thermal treatments simulating cooking processes on the polyphenol content in virgin olive oil. J Agric Food Chem 50, 59625967.
Bruneton, J (1995) Pharmacognosy Phytochemistry Medicinal Plants. Lavoisier Publishing: Secaucus, NY, pp. 218–219..
Budic-Leto, I, Lovric, T, Kljusuric, JG, Pezo, I & Vrhovsek, U (2006) Anthocyanin composition of the red wine Babic affected by maceration treatment. Eur Food Res Technol 222, 397402.
Bugianesi, R, Salucci, M, Leonardi, C, Ferracane, R, Catasta, G, Azzini, E & Maiani, G (2004) Effect of domestic cooking on human bioavailability of naringenin, chlorogenic acid, lycopene and ß-carotene in cherry tomatoes. Eur J Nutr 43, 360366.
Chesson, A, Provan, GJ, Russell, WR, Scobbie, L, Richardson, AJ & Stewart, C (1999) Hydroxycinnamic acids in the digestive tract of livestock and humans. J Sci Food Agric 79, 373378.
Couteau, D, McCartney, AL, Gibson, GR, Williamson, G & Faulds, CB (2001) Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. J Appl Microbiol 90, 873881.
Cremin, P, Kasim-Karakas, S & Waterhouse, AL (2001) LC/ES-MS detection of hydroxycinnamates in human plasma and urine. J Agric Food Chem 49, 17471750.
Di Venere, D, Linsalata, V, Calabrese, N, Pieralice, M, Bianco, VV & Lattanzio, V (2005) Morphological and biochemical changes during development of artichoke buds. Acta Hort 681, 437443, (ISHS).
Gebhardt, R (1997) Antioxidative and protective properties of extract from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicol Appl Pharm 144, 279286.
Gebhardt, R (1998) Inhibition of cholesterol biosyntesis in primary cultured rat hepatocytes by artichoke (Cynara scolymus L.) extracts. J Pharmacol Exp Ther 286, 11221128.
Gebhardt, R (2002) Inhibition of cholesterol biosyntesis HepG2 cells by artichoke extracts is reinforced by glucosidase pretreatment. Phytother Res 16, 368372.
Gumbinger, HG, Vahlensieck, U & Winterhoff, H (1993) Metabolism of caffeic acid in the isolated perfused rat liver. Planta Med 59, 491493.
Hertog, MGL, Hollman, CH & Venema, DP (1992) Optimization of quantitative HPLC determination of potentially anticarcenogenic flavonoids in vegetables and fruit. J Agric Food Chem 40, 15911598.
Hollman, PC, van der Gaag, M, Mengelers, MJ, van Trijp, JM, de Vries, JH & Katan, MB (1996) Absorption and disposition kinetics of the dietary antioxidant quercetin in man. Free Radic Biol Med 21, 703707.
Hollman, PC, van Trijp, JM, Buysman, MN, van der Gaag, MS, Mengelers, MJ, de Vries, JH & Katan, MB (1997) Relative bioavailability of the antioxidant flavonoid quercetin from various food in man. FEBS Lett 418, 152156.
Innocenti, M, Gallori, S, Giaccherini, C, Ieri, F, Vincieri, FF & Mulinacci, N (2005) Evaluation of the phenolic content in the aerial parts of different varieties of Cichorium intybus L. J Agric Food Chem 53, 64976502.
Ito, H, Gonthier, MP, Manach, C, Morand, C, Mennen, L, Rémésy, C & Scalbert, A (2005) Polyphenol levels in human urine after intake of six different polyphenol-rich beverages. Br J Nutr 94, 500509.
Kirchhoff, R, Beckers, C, Kirchhoff, GM, Trinczek-Gartner, H, Petrowitz, O & Reimann, HJ (1994) Increase in choleresis by means of artichoke extract. Results of a randomized placebo-controlled double-blind study. Phytomedicine 1, 107115.
Kraft, K (1997) Artichoke leaf extract. Recent findings reflecting effects on lipid metabolism, liver, and gastrointestinal tracts. Phytomedicine 4, 369378.
Lafay, S, Gil-Izquierdo, A, Manach, C, Morand, C, Besson, C & Scalbert, A (2006) Chlorogenic acid is absorbed in its intact form in the stomach of rats. J Nutr 136, 11921197.
Lattanzio, V & Van Sumere, CF (1987) Changes in phenolics during development and cold storage of artichoke (Cynara scolymus L.). Food Chemistry 24, 3750.
Lombard, K, Peffley, E, Geoffriau, E, Thompson, L & Herring, A (2005) Quercetin in onion (Allium cepa L.) after heat-treatment simulating home preparation. J Food Compost Anal 18, 571581.
Lopez-Molina, D, Navarro-Martinez, MD, Melgarejo, FR, Hiner, ANP, Chazarra, S & Rodríguez-Lopez, JN (2005) Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L.). Phytochemistry 66, 14761484.
McAnlis, GT, McEneny, J, Pearce, J & Young, IS (1999) Absorption and antioxidant effects of quercetin from onions in man. Eur J Clin Nutr 53, 9296.
Maiani, G, Serafini, M, Salucci, M, Azzini, E & Ferro-Luzzi, (1997) Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma. J Chrom B Biomed Sci Appl 692, 311317.
Manach, C, Scalbert, A, Morand, C, Rémésy, C & Jiménez, L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79, 727747.
Manach, C, Williamson, G, Morand, C, Scalbert, A & Rémésy, C (2005) Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 81, 230S242S.
Mannisto, PT, Tuomainen, P & Tuominen, RK (1992) Different in vivo properties of three new inhibitors of catechol-O-methyltransferase in the rat. Br J Pharmacol 105, 569574.
Masri, MS, Robbins, DJ, Emerson, OH & De Eds, F (1964) Selective para- or meta-O-methylation with catechol. O-methyl transferase from rat liver. Nature 202, 878879.
Miccadei, S, Bugianesi, R, Di Venere, D, Cardinali, A, Linsalata, V, Foddai, MS & Maiani, G (2004) Efficacia protettiva da danno ossidativi di frazioni polifenoliche da Cynara Scolymus in epatociti di ratto. Italus Hortus 11, 8689.
Moridani, MY, Scobie, H, Jamshidzadeh, A, Salehi, P & O'Brien, PJ (2001) Caffeic acid, chlorogenic acid, and dihydrocaffeic acid metabolism: glutathione conjugate formation. Drug Metab Dispos 29, 14321439.
Moridani, MY, Scobie, H & O'Brien, PJ (2002) Metabolism of caffeic acid by isolated rat hepatocytes and subcellular fractions. Toxicol Lett 133, 141151.
Nardini, M, Cirillo, E, Natella, F & Scaccini, C (2002) Absorption of phenolic acids in humans after coffee consumption. J Agric Food Chem 50, 57355741.
National Institute for Food and Nutrition Research (INRAN) (2000) Food Composition Tables. Rome, Italy: EDRA-Medical Publishing New Media.
Nissinen, E, Linden, IB, Schultz, E, Kaakkola, S, Mannisto, PT & Pohto, P (1988) Inhibition of catechol-O-methyltransferase activity by two novel disubstituted catechols in the rat. Eur J Pharmacol 153, 263269.
Olthof, MR, Hollman, PCH & Katan, MB (2001) Chlorogenic and caffeic acid are absorbed in humans. J Nutr 131, 6671.
Peppercorn, MA & Goldman, P (1971) Caffeic acid metabolism by bacteria of the human gastrointestinal tract. J Bacteriol 108, 9961000.
Plumb, GW, Garcia-Conesa, MT, Kroon, PA, Rodhes, M, Ridley, S & Williamson, G (1999) Metabolism of chlorogenic acid by human plasma, liver, intestine and gut microflora. J Sci Food Agric 79, 390392.
Ranganathan, S & Ramasarma, T (1974) The metabolism of phenolic acids in the rat. Biochem J 140, 517522.
Rechner, AR, Spencer, JPE, Kuhnle, G, Hahn, U & Rice-Evans, CA (2001 a) Novel biomarkers of the metabolism of caffeic acid derivatives in vivo. Free Rad Biol Med 30, 12131222.
Rechner, AR, Pannala, AS & Rice-Evans, CA (2001 b) Caffeic acid derivatives in artichoke extract are metabolised to phenolic acids in vivo. Free Rad Res 35, 195202.
Roberfroid, MB, Van Loo, JA & Gibson, GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 128, 1119.
Rubinskiene, M, Viskelis, P, Jasutiene, I, Viskeliene, R & Bobinas, C (2005) Impact of various factors on the composition and stability of black currant anthocyanins. Food Res Int 38, 867871.
Scalbert, A & Williamson, G (2000) Dietary intake and bioavailability of polyphenols. J Nutr 130, 2073S2085S.
Scheline, RR (1991) Metabolism of acids, lactones and esters. In Handbook of mammalian metabolism of plant compounds, pp. 168170Boca Ranton, USA: CRC Press.
Schultz, E & Nissinen, E (1989) Inhibition of rat liver and duodenum soluble catechol-O-methyltransferase by a tight-binding inhibitor OR-462. Biochem Pharmacol 38, 39533956.
Schütz, K, Kammerer, D, Carle, R & Schieber, A (2004) Identification and quantification of caffeoylquinic acids and flavonoids from artichoke (Cynara scolymus L.) heads, juice, and pomace by HPLC-DAD-ESI/MS. J Agric Food Chem 52, 40904096.
Shimoda, H, Ninomiya, K, Nishida, N, Yoshino, T, Morikawa, T, Matsuda, H & Yoshikawa, M (2003) Anti-hyperlipidemic sesquiterpenes and new sesquiterpene glycosides from the leaves of artichoke (Cynara scolymus L.): structure requirement and mode of action. Bioorg Med Chem Lett 13, 223228.
Speroni, E, Cervellati, R, Govoni, P, Guizzardi, S, Renzulli, C & Guerra, MC (2003) Efficacy of different Cynara scolymus preparations on liver complaints. J Ethnopharm 86, 203211.
Wang, M, Simon, JE, Aviles, IF, He, K, Zheng, QY & Tadmor, Y (2003) Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.). J Agric Food Chem 51, 601608.
Wittemer, SM, Ploch, M, Windeck, T, Müller, SC, Drewelow, B, Derendorf, H & Veit, M (2005) Bioavailability and pharmacokinetics of caffeoylquinic acids and flavonoids after oral administration of artichoke leaf extracts in humans. Phytomedicine 12, 2838.
Yang, H, Yuan, B, Li, L, Chen, H & Li, F (2004) HPLC determination and pharmacokinetics of chlorogenic acid in rabbit plasma after an oral dose of Flos Lonicerae extract. J Chromatogr Sci 42, 173176.
Zapolska-Downar, D, Zapolski-Downar, A, Naruszewicz, M, Siennicka, A, Krasnodębska, B & Kolodziej, B (2002) Protective properties of artichoke (Cynara scolymus) against ossidative stress induced in cultured endothelial cells and monocytes. Life Sci 71, 28972908.
Zhu, X & Zhang, H (2004) Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J Agric Food Chem 52, 72727278.

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