Skip to main content

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 11
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Giallourou, Natasa S. Rowland, Ian R. Rothwell, Steve D. Packham, Graham Commane, Daniel M. and Swann, Jonathan R. 2018. Metabolic targets of watercress and PEITC in MCF-7 and MCF-10A cells explain differential sensitisation responses to ionising radiation. European Journal of Nutrition,
    Williamson, Josh Hughes, Ciara and Davison, Gareth 2018. Exogenous Plant-Based Nutraceutical Supplementation and Peripheral Cell Mononuclear DNA Damage Following High Intensity Exercise. Antioxidants, Vol. 7, Issue. 5, p. 70.
    Klimek-Szczykutowicz, Marta Szopa, Agnieszka and Ekiert, Halina 2018. Chemical composition, traditional and professional use in medicine, application in environmental protection, position in food and cosmetics industries, and biotechnological studies of Nasturtium officinale (watercress) – a review. Fitoterapia,
    Karami, Masoumeh Mostafazadeh, Mostafa Sadeghi, Hossein Sadeghi, Heibatollah Mehraban, Fouad Panahi Kokhdan, Esmaeel Sayahi, Moslem and Abtahi, Seyed Reza 2018. Nephroprotective Effect of Nasturtium Officinale (Watercress) Ethanol Extract and Vitamin E on Vancomycin-Induced Nephrotoxicity in Rats. Jundishapur Journal of Natural Pharmaceutical Products, Vol. In Press, Issue. In Press,
    Palliyaguru, Dushani L. Yuan, Jian-Min Kensler, Thomas W. and Fahey, Jed W. 2018. Isothiocyanates: Translating the Power of Plants to People. Molecular Nutrition & Food Research, p. 1700965.
    Limón Pacheco, Jorge H. Carballo, Marta A. and Gonsebatt, María E. 2017. Nutritional Antioxidant Therapies: Treatments and Perspectives. p. 189.
    OKADA, HIROTAKA YOSHIDA, NORIKO KAKUMA, TATSUYUKI and TOYOMASU, KOUJI 2017. Effect of Chlorella Ingestion on Oxidative Stress and Fatigue Symptoms in Healthy Men. The Kurume Medical Journal, Vol. 64, Issue. 4, p. 83.
    Vicente, Joana G. Rothwell, Steve Holub, Eric B. and Studholme, David J. 2017. Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida. International Journal of Systematic and Evolutionary Microbiology, Vol. 67, Issue. 9, p. 3645.
    Davison, Gareth W. 2016. Exercise and Oxidative Damage in Nucleoid DNA Quantified Using Single Cell Gel Electrophoresis: Present and Future Application. Frontiers in Physiology, Vol. 7, Issue. ,
    Nikolaidis, Michalis Margaritelis, Nikos Paschalis, Vassilis Theodorou, Anastasios Kyparos, Antonios and Vrabas, Ioannis 2014. Antioxidants in Sport Nutrition. p. 221.
    FOGARTY, MARK C. DEVITO, GIUSEPPE HUGHES, CIARA M. BURKE, GEORGE BROWN, JOHN C. MCENENY, JANE BROWN, DAVID MCCLEAN, CONOR and DAVISON, GARETH W. 2013. Effects of α-lipoic Acid on mtDNA Damage after Isolated Muscle Contractions. Medicine & Science in Sports & Exercise, Vol. 45, Issue. 8, p. 1469.
    ×
  • Access

Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation

  • Mark C. Fogarty (a1), Ciara M. Hughes (a2), George Burke (a3), John C. Brown (a4) and Gareth W. Davison (a4)...
Abstract

Pharmacological antioxidant vitamins have previously been investigated for a prophylactic effect against exercise-induced oxidative stress. However, large doses are often required and may lead to a state of pro-oxidation and oxidative damage. Watercress contains an array of nutritional compounds such as β-carotene and α-tocopherol which may increase protection against exercise-induced oxidative stress. The present randomised controlled investigation was designed to test the hypothesis that acute (consumption 2 h before exercise) and chronic (8 weeks consumption) watercress supplementation can attenuate exercise-induced oxidative stress. A total of ten apparently healthy male subjects (age 23 (sd 4) years, stature 179 (sd 10) cm and body mass 74 (sd 15) kg) were recruited to complete the 8-week chronic watercress intervention period (and then 8 weeks of control, with no ingestion) of the experiment before crossing over in order to compete the single-dose acute phase (with control, no ingestion). Blood samples were taken at baseline (pre-supplementation), at rest (pre-exercise) and following exercise. Each subject completed an incremental exercise test to volitional exhaustion following chronic and acute watercress supplementation or control. The main findings show an exercise-induced increase in DNA damage and lipid peroxidation over both acute and chronic control supplementation phases (P< 0·05 v. supplementation), while acute and chronic watercress attenuated DNA damage and lipid peroxidation and decreased H2O2 accumulation following exhaustive exercise (P< 0·05 v. control). A marked increase in the main lipid-soluble antioxidants (α-tocopherol, γ-tocopherol and xanthophyll) was observed following watercress supplementation (P< 0·05 v. control) in both experimental phases. These findings suggest that short- and long-term watercress ingestion has potential antioxidant effects against exercise-induced DNA damage and lipid peroxidation.

  • View HTML
      • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation
      Available formats
      ×
Copyright
COPYRIGHT: © The Authors 2012
Corresponding author
*Corresponding author: Dr G. W. Davison, email gw.davison@ulster.ac.uk
References
Hide All
1Urso, ML & Clarkson, PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189, 41–54.
2Sacheck, JM, Milbury, PE, Cannon, JG, et al. (2003) Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free Radic Biol Med 34, 1575–1588.
3Clarkson, PM & Thompson, HS (2000) Antioxidants: what role do they play in physical activity and health. Am J Clin Nutr 72, 637–646.
4Finaud, J, Lac, G & Filaire, E (2006) Oxidative stress: relationship with exercise and training. Sports Med 4, 327–358.
5Bloomer, RJ, Fisher-Wellman, KH, Hammond, KG, et al. (2009) Dietary supplement increases plasma norepinephrine, lipolysis, and metabolic rate in resistance trained men. J Int Soc Sports Nutr 6, 10–19.
6Halliwell, B & Whiteman, M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Clin Pharmacol 142, 231–255.
7Vollaard, NB, Shearman, JP & Cooper, CE (2005) Exercise-induced oxidative stress: myths, realities and physiological relevance. Sports Med 35, 1045–1062.
8Mastaloudis, A, Morrow, JD, Hopkins, DW, et al. (2004) Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radic Biol Med 36, 1329–1341.
9Subudhi, AW, Jacobs, KA, Hagobian, TA, et al. (2004) Antioxidant supplementation does not attenuate oxidative stress at high altitude. Aviat Space Environ Med 75, 881–888.
10Davison, GW, Hughes, CM & Bell, RA (2005) Exercise and mononuclear cell DNA damage: the effects of antioxidant supplementation. Int J Sport Nutr Exerc Metab 15, 480–492.
11Kerksick, CM, Kreider, RB & Willoughby, DS (2010) Intramuscular adaptations to eccentric exercise and antioxidant supplementation. Amino Acids 39, 219–232.
12Vina, J, Gomez-Cabrera, MC & Borras, C (2010) Fostering antioxidant defences: up-regulation of antioxidant genes or antioxidant supplementation? Br J Nutr 98, 36–40.
13Lyall, KA, Hurst, SM, Cooney, JM, et al. (2009) Short-term blackcurrant extract consumption modulates exercise-induced oxidative stress and lipopolysaccharide-stimulated inflammatory responses. Am J Physiol Regul Integr Comp Physiol 297, 70–81.
14Steinkellner, H, Rabot, S, Freywald, C, et al. (2001) Effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutat Res 1, 285–297.
15Gill, CI, Haldar, S, Boyd, LA, et al. (2007) Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults. Am J Clin Nutr 85, 504–510.
16Boyd, LA, McCann, MJ, Hashim, Y, et al. (2006) Assessment of the anti-genotoxic, anti-proliferative, and anti-metastatic potential of crude watercress extract in human colon cancer cells. Nutr Cancer 55, 232–241.
17Samman, S, Sivarajah, G, Man, JC, et al. (2003) A mixed fruit and vegetable concentrate increases plasma antioxidant vitamins and folate and lowers plasma homocysteine in men. J Nutr 33, 2188–2193.
18Dill, DB & Costill, DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 1, 247–248.
19Singh, NP, McCoy, MT, Tice, RR, et al. (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175, 184–191.
20Collins, AR, Oscoz, AA, Brunborg, G, et al. (2008) The comet assay: topical issues. Mutagenesis 23, 143–151.
21Wolff, SP (1994) Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hypoperoxides. Methods Enzymol 233, 183–189.
22Thurnham, DI, Smith, E & Flora, PS (1988) Concurrent liquid-chromatographic assay of retinol, alpha-tocopherol, beta-carotene, alpha-carotene, lycopene, and beta-cryptoxanthin in plasma, with tocopherol acetate as internal standard. Clin Chem 34, 377–381.
23Altman, DG (1980) Statistics and ethics in medical research: III How large a sample? Br Med J 281, 1336–1338.
24Fogarty, MC, Hughes, CM, Burke, G, et al. (2011) Exercise-induced lipid peroxidation: implications for deoxyribonucleic acid damage and systemic free radical generation. Environ Mol Mutagen 52, 35–42.
25Hartmann, A, Plappert, U, Raddatz, K, et al. (1994) Does physical activity induce DNA damage? Mutagenesis 9, 269–272.
26Poulsen, HE, Loft, S & Vistisen, K (1996) Extreme exercise and oxidative DNA modification. J Sports Sci 14, 343–346.
27Radak, Z, Apor, P, Pucsok, J, et al. (2003) Marathon running alters the DNA base excision repair in human skeletal muscle. Life Sci 72, 1627–1633.
28Gomez-Cabrera, MC, Vina, J & Ji, LL (2009) Interplay of oxidants and antioxidants during exercise: implications for muscle health. Phys Sportsmed 37, 116–123.
29Nikolaidis, MG & Jamurtas, AZ (2009) Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys 490, 77–84.
30Pedersen, BK, Rohde, T & Zacho, M (1996) Immunity in athletes. J Sports Med Phys Fitness 36, 236–245.
31Niess, AM, Baumann, M, Roecker, K, et al. (1998) Effects of intensive endurance exercise on DNA damage in leucocytes. J Sports Med Phys Fitness 38, 111–115.
32Guetens, G, De Boeck, G, Highley, M, et al. (2002) Oxidative DNA damage: biological significance and methods of analysis. Crit Rev Clin Lab Sci 39, 331–457.
33Powers, SK & Jackson, MJ (2008) Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 88, 1243–1276.
34Radak, Z & Goto, S (2000) Oxidative modification of proteins and DNA. In Free Radicals in Exercise and Aging, 1st ed., pp. 177 [Radak, Z, editor]. Champaign, IL: Human Kinetics.
35Bracken, RM, Linnane, DM & Brooks, S (2009) Plasma catecholamine and nephrine responses to brief intermittent maximal intensity exercise. Amino Acids 36, 209–217.
36Halliwell, B & Gutteridge, J (2007) Free Radicals in Biology and Medicine, 4th ed.London: Oxford University Press.
37Davison, GW, Morgan, RM, Hiscock, N, et al. (2006) Manipulation of systemic oxygen flux by acute exercise and normobaric hypoxia: implications for reactive oxygen species generation. Clin Sci 110, 133–141.
38Davison, GW, Ashton, T, Davies, B, et al. (2008) In vitro electron paramagnetic resonance characterisation of free radical: relevance to exercise-induced lipid peroxidation and implications of ascorbate prohylaxis. Free Radic Res 42, 1–8.
39Marnett, LJ (2002) Oxy radicals, lipid peroxidation and DNA damage. Toxicology 181, 219–222.
40Lushchak, VI (2007) Free radical oxidation of proteins and its relationship with functional state of organisms. Biochemistry Mosc 72, 809–827.
41Alessio, HM, Hagerman, AE, Fulkerson, BK, et al. (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 32, 1576–1581.
42Bloomer, RJ, Goldfarb, AH, Wideman, L, et al. (2005) Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 19, 276–285.
43O'Neill, ME, Carroll, Y, Corridan, B, et al. (2001) A European carotenoid database to assess carotenoid intakes and its use in a five-country comparative study. Br J Nutr 85, 499–507.
44Conaway, CC, Yang, YM & Chung, FL (2002) Isothiocyanates as cancer chemopreventive agents: their biological activities and metabolism in rodents and humans. Curr Drug Metab 3, 233–255.
45Sies, H & Stahl, W (1995) Vitamins E and C, beta-carotene, and other carotenoids as antioxidants. Am J Clin Nutr 62, 1315–1321.
46Packer, L, Witt, EH & Tritschler, HJ (1995) Alpha-lipoic acid as a biological antioxidant. Free Radic Biol Med 19, 227–250.
47Haegele, AD, Gillette, C & O'Neill, C (2000) Plasma xanthophyll carotenoids correlate inversely with indices of oxidative DNA damage and lipid peroxidation. Cancer Epidemiol Biomarkers Prev 9, 421–425.
48Long, W 3rd, Wells, K, Englert, V, et al. (2008) Does prior acute exercise affect post exercise substrate oxidation in response to a high carbohydrate meal? Nutr Metab 5, 2–5.
49Pincemail, J, Deby, C, Camus, G, et al. (1988) Tocopherol mobilisation during intensive exercise. Eur J Appl Physiol 57, 189–191.
50Davison, GW, George, L, Jackson, SK, et al. (2002) Exercise, free radicals, and lipid peroxidation in type 1 diabetes mellitus. Free Radic Biol Med 33, 1543–1551.
51McClean, C, Clegg, M, Shafat, A, et al. (2011) The impact of acute moderate intensity exercise on arterial regional stiffness, lipid peroxidation and antioxidant status in healthy males. Res Sports Med 19, 1–13.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Keywords

Metrics

Altmetric attention score

Cancel
Confirm
×