Skip to main content
    • Aa
    • Aa

Anti-ageing effect of a lactococcal strain: analysis using senescence-accelerated mice

  • Hiromi Kimoto-Nira (a1), Chise Suzuki (a1), Miho Kobayashi (a1), Keisuke Sasaki (a1), Jun-ichi Kurisaki (a2) and Koko Mizumachi (a1)...

The effects of oral administration of a lactococcal strain on physiological changes associated with ageing were investigated using senescence-accelerated mice (SAM). SAM develop normally, but then show an early onset and irreversible advancement of senescence. SAMP6 is a SAM strain that develops osteoporosis with ageing. Oral administration of heat-killed Lactococcus lactis subsp. cremoris H61 (strain H61) to aged SAMP6 mice was associated with reduced bone density loss, a suppression of incidence of skin ulcers and reduced hair loss, compared with controls. Spleen cells from mice fed strain H61 produced more interferon-γ and IL-12 than those from control mice, suggesting that administration of strain H61 altered immune responses. The numbers of viable cells of Bifidobacterium sp., Bacteroides sp. and Enterococcus sp. in faeces were similar for mice fed the strain H61 and control diets, but counts for Staphylococcus sp. were significantly lower (P < 0·05) in mice fed strain H61. Mice fed strain H61 had similar serum concentrations of thiobarbituric acid-reactive substances as in controls, indicating a lack of effect on lipid peroxidation status. Administration of living cells of strain H61 or fermented milk containing strain H61 was also associated with a suppression of incidence of skin ulcers and reduced hair loss. These results indicate that oral administration of strain H61 has the potential to suppress some of the manifestations associated with ageing.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Anti-ageing effect of a lactococcal strain: analysis using senescence-accelerated mice
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Anti-ageing effect of a lactococcal strain: analysis using senescence-accelerated mice
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Anti-ageing effect of a lactococcal strain: analysis using senescence-accelerated mice
      Available formats
Corresponding author
*Corresponding author: Dr H. Kimoto-Nira, fax +81 298388606, email
Hide All
1Lee YK & Salminen S (1995) The coming of age of probiotics. Trends Food Sci Technol 6, 241245.
2Salminen S, Ouwehand A, Benno Y & Lee YK (1999) Probiotics: how should they be defined? Trends Food Sci Technol 10, 107110.
3De Rodas BZ, Gilliland SE & Maxwell CV (1996) Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J Dairy Sci 79, 21212128.
4Lidbeck A, Geltner-Allinger U, Orrhage KM, Ottova L, Brismar B, Gustafsson JA, Rafter JJ & Nord CE (1991) Impact of Lactobacillus acidophilus supplements on the faecal microflora and soluble faecal bile acids in colon cancer patients. Microbiol Ecol Health Dis 4, 8188.
5Kalliomaki M, Salminen S, Poussa T, Arvilommi H & Isolauri E (2003) Probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial. Lancet 361, 18691871.
6Kimoto H, Kurisaki J, Tsuji NM, Ohmomo S & Okamoto T (1999) Lactococci as probiotic strains: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile. Lett Appl Microbiol 29, 313316.
7Kimoto H, Ohmomo S, Nomura M, Kobayashi M & Okamoto T (2000) In vitro studies on probiotic properties of lactococci. Milchwissenschaft 55, 245249.
8Kimoto H, Ohmomo S & Okamoto T (2002) Cholesterol removal from media by lactococci. J Dairy Sci 85, 31823188.
9Kimoto H, Mizumachi K, Okamoto T & Kurisaki J (2004) New Lactococcus strain with immunomodulatory activity: enhancement of Th1-type immune response. Microbiol Immunol 48, 7582.
10Nomura M, Kimoto H, Someya Y, Furukawa S & Suzuki I (1998) Production of gamma-aminobutyric acid by cheese starters during cheese ripening. J Dairy Sci 81, 14861491.
11Nomura M, Kimoto H, Someya Y & Suzuki I (1999) Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris. Int J Syst Bacteriol 49, Pt 1, 163166.
12Inoue K, Shirai T, Ochiai H, Kasao M, Hayakawa K, Kumura M & Sansawa H (2003) Blood-pressure-lowering effect of a novel fermented milk containing gamma-aminobutyric acid (GABA) in mild hypertensives. Eur J Clin Nutr 57, 490495.
13Hayakawa K, Kimura M, Kasaha K, Matsumoto K, Sansawa H & Yamori Y (2004) Effect of a gamma-aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hypertensive and normotensive Wistar-Kyoto rats. Br J Nutr 92, 411417.
14Mahncke HW, Bronstone A & Merzenich MM (2006) Brain plasticity and functional losses in the aged: scientific bases for a novel intervention. Prog Brain Res 157, 81109.
15Solana R & Mariani E (2000) NK and NK/T cells in human senescence. Vaccine 18, 16131620.
16Voss P & Siems W (2006) Clinical oxidation parameters of aging. Free Radic Res 40, 13391349.
17Stock H, Schneider A & Strauss E (2004) Osteoporosis: a disease in men. Clin Orthop Relat Res Aug.: 425, 143151.
18Lei H, Wang B, Li WP, Yang Y, Zhou AW & Chen MZ (2003) Anti-aging effect of astragalosides and its mechanism of action. Acta Pharmacol Sin 24, 230234.
19Kim SY, Kim SJ, Lee YJ, Kim WG, Park WS, Sim YC & Lee SJ (2004) Protective effects of dietary soy isoflavones against UV-induced skin-aging in hairless mouse model. J Am Coll Nutr 23, 157162.
20Rubino C, Farace F, Dessy LA, Sanna MP & Mazzarello V (2005) A prospective study of anti-aging topical therapies using a quantitative method of assessment. Plast Reconstr Surg 115, 11561162.
21Kim JH, Lee BC, Kim JH, Sim GS, Lee DH, Lee KE, Yun YP & Pyo HB (2005) The isolation and antioxidative effects of vitexin from Acer palmatum. Arch Pharm Res 28, 195202.
22Hori T, Kiyoshima J, Shida K & Yasui H (2002) Augmentation of cellular immunity and reduction of influenza virus titer in aged mice fed Lactobacillus casei strain Shirota. Clin Diagn Lab Immunol 9, 105108.
23Matsumoto S, Watanabe N, Imaoka A & Okabe Y (2001) Preventive effects of Bifidobacterium-and Lactobacillus-fermented milk on the development of inflammatory bowel disease in senescence-accelerated mouse P1/Yit strain mice. Digestion 64, 9299.
24Sambrook P & Cooper C (2006) Osteoporosis. Lancet 367, 20102018.
25Takeda T, Hosokawa M, Takeshita S, et al. (1981) A new murine model of accelerated senescence. Mech Ageing Dev 17, 183194.
26Ohata H, Nishikawa H, Hirai K, Kato K & Miyamoto M (1996) Relationship of impaired brain glucose metabolism to learning deficit in the senescence-accelated mouse. Neurosci Lett 217, 3740.
27Li L, Ng TB, Gao W, Li W, Fu M, Niu SM, Zhao L, Chen RR & Liu F (2005) Antioxidant activity of gallic acid from rose flowers in senescence accelerated mice. Life Sci 77, 230240.
28Hosokawa M, Kasai R, Higuchi K, et al. (1984) Grading score system: a method for evaluation of the degree of senescence in senescence accelerated mouse (SAM). Mech Ageing Dev 26, 91102.
29Rogosa M, Mitchell JA & Wiseman RF (1951) A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J Bacteriol 62, 132133.
30Wozniak A, Wozniak B, Drewa G & Drewa T (2003) Lipid peroxidation and antioxidant capacity in selected tissues of healthy black C57BL/6J mice and B16 melanoma-bearing mice. Melanoma Res 13, 1922.
31Naito M & Yamanaka K (1978) Arteriosclerosis and hydroperoxide. Nihon Rohnen Igakuzasshi 15, 187191.
32Faloni AP, Sasso-Cerri E, Katchburian E & Cerri PS (2007) Decrease in the number and apoptosis of alveolar bone osteoclasts in estrogen-treated rats. J Priodontal Res 42, 193201.
33Huth PJ, DiRienzo DB & Miller GD (2006) Major scientific advances with dairy foods in nutrition and health. J Dairy Sci 89, 12071221.
34Wildner M, Peters A, Raghuvanshi VS, Hohnloser J & Siebert U (2003) Superiority of age and weight as variables in predicting osteoporosis in postmenopausal white women. Osteoporos Int 14, 950956.
35Lappin MB & Campbell JD (2000) The Th1-Th2 classification of cellular immune responses: concepts, current thinking and applications in haematological malignancy. Blood Rev 14, 228239.
36Mascarucci P, Taub D, Saccani S, Paloma MA, Dawson H, Roth GS, Ingram DK & Lane MA (2001) Age-related changes in cytokine production by leukocytes in rhesus monkeys. Aging (Milan) 13, 8594.
37Trinchieri G (1994) Interleukin-12: a cytokine produced by antigen-presenting cells with immunoregulatory functions in the generation of T-helper cells type 1 and cytotoxic lymphocytes. Blood 84, 40084027.
38Skurkovich S & Skurkovich B (2005) Anticytokine therapy, especially anti-interferon-gamma, as a pathogenetic treatment in TH-1 autoimmune diseases. Ann N Y Acad Sci 1051, 684700.
39Inanir A, Ozoran K, Tutkak H & Mercerci B (2004) The effects of calcitriol therapy on serum interleukin-1, interleukin-6 and tumor necrosis factor-alpha concentrations in post-menopausal patients with osteoporosis. J Int Med Res 32, 570582.
40Blair HC, Robinson LJ & Zaidi M (2005) Osteoclast signalling pathways. Biochem Biophys Res Commun 328, 728738.
41Mitsuoka T (1992) Intestinal flora and aging. Nutr Rev 50, 438446.
42Coconnier MH, Bernet MF, Kerneis S, Chauviere G, Fourniat J & Servin AL (1993) Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS Microbiol Lett 110, 299305.
43Beckman KB & Ames BN (1998) The free radical theory of aging matures. Physiol Rev 78, 547581.
44Salminen S, von Wright A, Morelli L, et al. (1998) Demonstration of safety of probiotics – a review. Int J Food Microbiol 44, 93106.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 14
Total number of PDF views: 128 *
Loading metrics...

Abstract views

Total abstract views: 208 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th October 2017. This data will be updated every 24 hours.