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Bioavailability of β-carotene (βC) from purple carrots is the same as typical orange carrots while high-βC carrots increase βC stores in Mongolian gerbils(Meriones unguiculatus)

Published online by Cambridge University Press:  08 March 2007

Mandy Porter Dosti
Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
Jordan P. Mills
Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
Philipp W. Simon
United States Department of Agriculture Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA
Sherry A. Tanumihardjo*
Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
*Corresponding author: Dr Sherry A. Tanumihardjo, fax +1 608 262 5860, email
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Vitamin A (VA) deficiency is a worldwide public health problem. Biofortifying existing sources of β-carotene (βC) and increasing dietary βC could help combat the issue. Two studies were performed to investigate the relative βC bioavailability of a βC supplement to purple, high-βC orange, and typical orange carrots using Mongolian gerbils (Meriones unguiculatus). In study 1, which used a traditional bioavailability design, gerbils (n32) received a diet containing orange, purple, or white carrot powder, or white carrot powder +a βC supplement. In study 2, which included βC-biofortified carrots, gerbils (n 39) received orange, high-βC orange, purple, or white carrot powder in their diet. Both studies lasted 21 d and the gerbils were killed to determine the effect of carrot type or supplement on serum and liver βC, α-carotene, and VA concentrations. Liver stores of βC or VA in the gerbils did not differ between orange and purple carrot diets when equal amounts of βC from each of the diets were consumed (P>0·05). Both the orange and purple carrot diet resulted in higher liver VA compared with the supplement (P<0·05). High-βC carrots resulted in more than 2-fold higher βC and 1·1 times greater VA liver stores compared with typical orange carrots (P<0·05). These results suggest that high-βC carrots may be an alternative source of VA to typical carrots in areas of VA deficiency. Second, phenolics including anthocyanins and phenolic acids in purple carrot do not interfere with the bioavailability of βC from purple carrots.

Research Article
Copyright © The Nutrition Society 2006


Alasalvar, C, Grigor, JM, Zhang, D, Quantick, PC & Shahidi, FComparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. J Agric Food Chem (2001) 49 14101416CrossRefGoogle ScholarPubMed
Brown, ED, Micozzi, MS,Craft, NE,Bieri, UG, Beecher, G, Edwards, BR,Rose, A, Taylor, PR & Smith, JC JrPlasma carotenoids in normal men after a single ingestion of vegetables or purified β-carotene. Am J Clin Nutr (1989) 49 12581265CrossRefGoogle ScholarPubMed
Canfield, LM, Guiliano, AR, Neilson, EM, Yap, HH, Graver, EJ, Cui, HA & Blashill, BMβ-Carotene in breast milk and serum is increased after a single β-carotene dose Am J Clin Nutr (1997) 66 5261CrossRefGoogle ScholarPubMed
Castenmiller, JJM & West, CEBioavailability and bioconversion of carotenoids. Annu Rev Nutr (1998) 18 1938CrossRefGoogle ScholarPubMed
Chapman, JMComparative analysis of mammalian plasma lipoproteins. In Methods in Enzymology, [Segrest, JP and Albers, JJ] New York: Academic Press (1986) 70143Google ScholarPubMed
Criqui, MH & Ringel, BLDoes diet or alcohol explain the French paradox?. Lancet (1994) 344 17191723CrossRefGoogle ScholarPubMed
Deming, DM, Boileau, AC, Lee, CM & Erdman, JW JrAmount of dietary fat and type of soluble fiber independently modulate postabsorptive conversion of βcarotene to vitamin A in Mongolian gerbils. J Nutr (2000) 130 27892796CrossRefGoogle ScholarPubMed
DeRitter, E, Purcell, AECarotenoid analytical methods. InCarotenoids as Colorants and Vitamin A Precursors: Technical and Nutritional Applications [Bauernfeind, JC] New York: Academic Press (1981) 889Google Scholar
Erdman, JW Jr, Bierer, TL & Gugger, ETAbsorption and transport of carotenoids. Ann NY Acad Sci (1993) 691 7685CrossRefGoogle ScholarPubMed
Escaron, AL & Tanumihardjo, SA (2006) Absorption and transit of lutein and b-carotene supplements in the mongolian gerbil (Meriones unguiculatus). Int J Vit Nutr Res (in the press).CrossRefGoogle Scholar
Furusho, T, Kataoka, E, Yasuhara, T, Wada, M & Masushige, SRetinol equivalence of carotenoids can be evaluated by hepatic vitamin A content. Int J Vitam Nutr Res (2000) 70 4347CrossRefGoogle ScholarPubMed
Glabgen, WE, Seitz, HU & Metzger, JWHigh-performance liquid chromatography/electrospray mass spectrometry and tandem mass spectrometry of anthocyanins from plant tissues and cell cultures of Daucus carota L. Biol Mass Spectrom (1992 a) 21 271277CrossRefGoogle Scholar
Glabgen, WE, Wray, V, Strack, D, Metzger, JW & Seitz, HUAnthocyanins from cell suspension cultures of Daucus Carota. Phytochemistry (1992 b) 31 15931601Google Scholar
Guisti, MM, Rodríguez-Saona, LE, Griffin, D & Wrolstad, REElectrospray and tandem mass spectrometry as tools for anthocyanin characterization. J Agric Food Chem (1999 a) 47 46574664CrossRefGoogle Scholar
Guisti, MM, Rodríguez-Saona, LE & Wrolstad, REMolar absorptivity and color characteristics of acylated and non-acylated pelargonidin-based anthocyanins. J Agric Food Chem (1999 b) 47 46314637CrossRefGoogle Scholar
Guisti, MM & Wrolstad, RERadish anthocyanin extract as a natural red colorant for maraschino cherries. J Food Sci (1996) 61 688694CrossRefGoogle Scholar
Harborne, JBA unique pattern of anthocyanins in Daucus carota and other umbelliferae. Biochem Syst Ecol (1976) 4 3135CrossRefGoogle Scholar
Harborne, JBIdentification of the major anthocyanin of carrot cells in tissue culture as cyanidin 3-(sinapoylxloslylglucosylgalactoside). Z Naturforsch (1983) 38C 10551056Google Scholar
Harborne, JBThe anthocyanins. In The Flavonoids: Advances in Research Since 1980, [Harborne, JB] NewYork: Chapman and Hall (1988) 120Google Scholar
Horvitz, MA, Simon, PW & Tanumihardjo, SALycopene and β-carotene are bioavailable from lycopene ‘red’ carrots in humans. Eur J Clin Nutr (2004) 58 803811CrossRefGoogle ScholarPubMed
House, WA, Apgar, J & Smith, JCThe gerbil: a model for studying the metabolism of beta-carotene and minerals. Nutr Res (1997) 17 12931302CrossRefGoogle Scholar
International Vitamin A Consultative Group The Bioavailability of Dietary Carotenoids: Current Concepts. Washington, DC: ILSI (1999)Google Scholar
Lee, CM, Boileau, AC, Boileau, TWM, Williams, AW, Swaroon, KS, Heintz, KA & Erdman, JW JrReview of animal models in carotenoid research. J Nutr (1999) 129 22712277CrossRefGoogle ScholarPubMed
Lee, CM, Lederman, JD, Hofmann, NE & Erdman, JW JrThe Mongolian gerbil (Meriones unguiculatus) is an appropriate animal for evaluation of the conversion of β-carotene to vitamin A. J Nutr (1998) 128 280286CrossRefGoogle ScholarPubMed
Malien-Auburt, C, Dangles, O & Amiot, MJColor stability of commercial anthocyanin-based extracts in relation to the phenolic composition. Protective effects by intra- and intermolecular copigmentation. J Agric Food Chem (2001) 49 170176CrossRefGoogle Scholar
Micozzi, MS, Brown, ED, Edwards, BK, Bieri, JG, Taylor, PR, Khachik, F, Bucher, GR & Smith, JC JrPlasma carotenoid response to chronic intake of selected foods and β-carotene supplements in men. Am J Clin Nutr (1992) 55 11201125CrossRefGoogle ScholarPubMed
Nicolosi, RJ, Marlett, JA, Morello, AM, Flanagan, SA & Hegsted, DMInfluence of dietary unsaturatedandsaturatedfat onthe plasmalipoproteins of Mongolian gerbils. Atherosclerosis (1981) 38 359371CrossRefGoogle Scholar
Olson, JAVitamin A. In Handbook of Vitamins, [Machlin, LJ] New York: Marcel Dekker (1991) 157Google ScholarPubMed
Pollack, J, Campbell, JM, Potter, SM & Erdman, JW JrMongolian gerbils (Meriones unguiculatus) absorb β-carotene intact from a test meal. J Nutr (1994) 124 869873CrossRefGoogle ScholarPubMed
Renaud, S & de Lorgeril, MWine, alcohol, platelets, and the French paradox for coronary heart disease Lancet (1992) 339 15231526CrossRefGoogle ScholarPubMed
Simon, PWCarrots and other horticultural crops as a source of provitamin A carotenes HortScience (1990) 25 14951499Google Scholar
Simon, PWB7262, purple carrot inbred. HortScience (1997 a) 32 146147Google Scholar
Simon, PWPlant pigments for color and nutrition. HortScience (1997 b) 32 1213Google Scholar
Simon, PW, Wolff, XY, Peterson, CE & Kammerlohr, DSHigh carotene mass carrot population. HortScience (1989) 24 174175Google Scholar
Singleton, VI & Rossi, JAColorimetry of total phenolic with phosphomolybdic-phosphotungstic acid reagents. J Enol Viticul (1965) 16 144158Google Scholar
Tanumihardjo, SAFactors influencing the conversion of carotenoids to retinol: bioavailability to bioconversion to bioefficacy. Int J Vitam Nutr Res (2002) 72 4045CrossRefGoogle ScholarPubMed
Tanumihardjo, SAAssessing vitamin A status: past, present and future. J Nutr (2004) 134 290S293SCrossRefGoogle ScholarPubMed
Tanumihardjo, SA, Furr, HC, Amedee-Manesme, O & Olson, JARetinyl ester (vitamin A ester) and carotenoid composition in human liver. Int J Vitam Nutr Res (1990) 60 307313Google ScholarPubMed
Thatcher, AJ, Lee, CM & Erdman, JW JrTissue stores of β-carotene are not conserved for later use as a source of vitamin A during compromised vitamin A status in Mongolian gerbils (Meriones unguiculatus). J Nutr (1998) 128 11791185CrossRefGoogle Scholar
van het Hof, KH, West, CE,Weststrate, JA & Hautvast, JGAJDietary factors that affect the bioavailability of carotenoids. J Nutr (2000) 130 503506CrossRefGoogle ScholarPubMed
World Health Organization (2003) Micronutrient deficiencies. Combating vitamin A deficiency. The Challenge. Scholar
Yeum, K & Russell, RMCarotenoid bioavailability and bioconversion. Annu Rev Nutr (2002) 22 483504CrossRefGoogle ScholarPubMed