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Comparative effect of orally administered sodium butyrate before or after weaning on growth and several indices of gastrointestinal biology of piglets

  • Maud Le Gall (a1), Mélanie Gallois (a2), Bernard Sève (a1), Isabelle Louveau (a1), Jens J. Holst (a3), Isabelle P. Oswald (a2), Jean-Paul Lallès (a1) and Paul Guilloteau (a1)...
Abstract

Sodium butyrate (SB) provided orally favours body growth and maturation of the gastrointestinal tract (GIT) in milk-fed pigs. In weaned pigs, conflicting results have been obtained. Therefore, we hypothesised that the effects of SB (3 g/kg DM intake) depend on the period (before v. after weaning) of its oral administration. From the age of 5 d, thirty-two pigs, blocked in quadruplicates within litters, were assigned to one of four treatments: no SB (control), SB before (for 24 d), or after (for 11–12 d) weaning and SB before and after weaning (for 35–36 d). Growth performance, feed intake and various end-point indices of GIT anatomy and physiology were investigated at slaughter. The pigs supplemented with SB before weaning grew faster after weaning than the controls (P < 0·05). The feed intake was higher in pigs supplemented with SB before or after weaning (P < 0·05). SB provided before weaning improved post-weaning faecal digestibility (P < 0·05) while SB after weaning decreased ileal and faecal digestibilities (P < 0·05). Gastric digesta retention was higher when SB was provided before weaning (P < 0·05). Post-weaning administration of SB decreased the activity of three pancreatic enzymes and five intestinal enzymes (P < 0·05). IL-18 gene expression tended to be lower in the mid-jejunum in SB-supplemented pigs. The small-intestinal mucosa was thinner and jejunal villous height lower in all SB groups (P < 0·05). In conclusion, the pre-weaning SB supplementation was the most efficient to stimulate body growth and feed intake after weaning, by reducing gastric emptying and intestinal mucosa weight and by increasing feed digestibility.

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*Corresponding author: Dr Maud Le Gall, fax +33 223485080, email maud.legall@rennes.inra.fr
References
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1Cummings JH & Macfarlane GT (1991) The control and consequences of bacterial fermentation in the human colon. J Appl Bacteriol 70, 443459.
2Sun CQ, O'Connor CJ & Roberton AM (2002) The antimicrobial properties of milkfat after partial hydrolysis by calf pregastric lipase. Chem Biol Interact 140, 185198.
3Roediger WE (1980) The colonic epithelium in ulcerative colitis: an energy-deficiency disease? Lancet 316, 712715.
4Cook SI & Sellin JH (1998) Review Article: short chain fatty acids in health and disease. Aliment Pharmacol Ther 12, 499507.
5Mariadason JM, Kilias D, Catto-Smith A, et al. (1999) Effect of butyrate on paracellular permeability in rat distal colonic mucosa ex vivo. J Gastroenterol Hepatol 14, 873879.
6Kinoshita M, Suzuki Y & Saito Y (2002) Butyrate reduces colonic paracellular permeability by enhancing PPARγ activation. Biochem Biophys Res Commun 293, 827831.
7Koruda MJ, Rolandelli RH, Bliss DZ, et al. (1990) Parenteral nutrition supplemented with short-chain fatty acids: effect on the small-bowel mucosa in normal rats. Am J Clin Nutr 51, 685689.
8Scheppach W & Weiler F (2004) The butyrate story: old wine in new bottles? Curr Opin Clin Nutr Metab Care 7, 563567.
9Weber TE & Kerr BJ (2006) Butyrate differentially regulates cytokines and proliferation in porcine peripheral blood mononuclear cells. Vet Immunol Immunopathol 113, 139147.
10Segain JP, Raingeard de la Blètiere D, Bourreille A, et al. (2000) Butyrate inhibits inflammatory responses through NFκB inhibition: implications for Crohn's disease. Gut 47, 397403.
11Venkatraman A, Ramakrishna BS, Shaji RV, et al. (2003) Amelioration of dextran sulfate colitis by butyrate: role of heat shock protein 70 and NF-κB. Am J Physiol Gastrointest Liver Physiol 285, G177G184.
12Pender SL, Quinn JJ, Sanderson IR, et al. (2000) Butyrate upregulates stromelysin-1 production by intestinal mesenchymal cells. Am J Physiol Gastrointest Liver Physiol 279, G918G924.
13Inagaki A & Sakata T (2005) Dose-dependent stimulatory and inhibitory effects of luminal and serosal n-butyric acid on epithelial cell proliferation of pig distal colonic mucosa. J Nutr Sci Vitaminol 51, 156160.
14Velazquez OC, Lederer HM & Rombeau JL (1996) Butyrate and the colonocyte. Implications for neoplasia. Dig Dis Sci 41, 727739.
15Kien CL, Schmitz-Brown M, Solley T, et al. (2006) Increased colonic luminal synthesis of butyric acid is associated with lowered colonic cell proliferation in piglets. J Nutr 136, 6469.
16Kien CL, Blauwiekel R, Bunn JY, et al. (2007) Cecal infusion of butyrate increases intestinal cell proliferation in piglets. J Nutr 137, 916922.
17Gálfi P & Bokori J (1990) Feeding trial in pigs with a diet containing sodium n-butyrate. Acta Vet Hung 38, 317.
18Piva A, Morlacchini M, Casadei G, et al. (2002) Sodium butyrate improves growth performance of weaned piglets during the first period after weaning. It J Anim Sci 1, 3541.
19Manzanilla EG, Nofrarias M, Anguita M, et al. (2006) Effects of butyrate, avilamycin, and a plant extract combination on the intestinal equilibrium of early-weaned pigs. J Anim Sci 84, 27432751.
20Biagi G, Piva A, Moschini M, et al. (2007) Performance, intestinal microflora, and wall morphology of weanling pigs fed sodium butyrate. J Anim Sci 85, 11841191.
21Kotunia A, Woliński J, Laubitz D, et al. (2004) Effect of sodium butyrate on the small intestine development in neonatal piglets fed by artificial sow. J Physiol Pharmacol 55, Suppl. 2, 5968.
22Guilloteau P, Zabielski R, David JC, et al. (2009) Sodium-butyrate as a growth promoter in milk replacer formula for young calves. J Dairy Sci 92, 10381049.
23Mazzoni M, Le Gall M, De Filippi S, et al. (2008) Supplemental sodium butyrate stimulates different gastric cells in weaned pigs. J Nutr 138, 14261431.
24Noblet J & Etienne M (1989) Estimation of sow milk nutrient output. J Anim Sci 67, 33523359.
25InraPorc (2009) InraPorc® – a tool to evaluate nutritional strategies in pigs.http://w3.rennes.inra.fr/inraporc/EN/index.html.
26Wilfart A, Montagne L, Simmins PH, et al. (2007) Sites of nutrient digestion in growing pigs: effect of dietary fiber. J Anim Sci 85, 976983.
27Association of Official Analytical Chemists (1999) Official Methods of Analysis, 16th ed.Gaithersburg, MD: AOAC.
28Poncet C & Rayssiguier Y (1980) Effect of lactose supplement on digestion of lucerne hay by sheep 1. J Anim Sci 51, 180185.
29Njaa LR (1961) Determination of protein digestibility with titanium dioxide as indicator substance. Acta Agric Scand 11, 227241.
30Goodlad RA, Levi S, Lee CY, et al. (1991) Morphometry and cell proliferation in endoscopic biopsies: evaluation of a technique. Gastroenterology 101, 12351241.
31Boudry G, Peron V, Le Huerou-Luron I, et al. (2004) Weaning induces both transient and long-lasting modifications of absorptive, secretory, and barrier properties of piglet intestine. J Nutr 134, 22562262.
32Domeneghini C, Di Giancamillo A, Bosi G, et al. (2006) Can nutraceuticals affect the structure of intestinal mucosa? Qualitative and quantitative microanatomy in l-glutamine diet-supplemented weaning piglets. Vet Res Commun 30, 331342.
33Piel C, Montagne L, Sève B, et al. (2005) Increasing digesta viscosity using carboxymethylcellulose in weaned piglets stimulates ileal goblet cell numbers and maturation. J Nutr 135, 8691.
34Lowry OH, Rosebrough NJ, Farr AL, et al. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265275.
35Gestin M, Le Huërou-Luron I, Péniau J, et al. (1997) Method of measurement of pancreatic elastase II activity in human duodenal juice and, bovine and porcine pancreatic tissue. Dig Dis Sci 42, 13021311.
36Montagne L, Boudry G, Favier C, et al. (2007) Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning. Br J Nutr 97, 4557.
37Marion J, Petersen YM, Romé V, et al. (2005) Early weaning stimulates intestinal brush border enzyme activities in piglets, mainly at the posttranscriptional level. J Pediatr Gastroenterol Nutr 41, 401410.
38Meissonnier GM, Pinton P, Laffitte J, et al. (2008) Immunotoxicity of aflatoxin B1: impairment of the cell-mediated response to vaccine antigen and modulation of cytokine expression. Toxicol Appl Pharmacol 231, 142149.
39Oswald IP, Dozois CM, Barlagne R, et al. (2001) Cytokine mRNA expression in pigs infected with Schistosoma japonicum. Parasitology 122, 299307.
40Von der Hardt K, Kandler MA, Fink L, et al. (2004) High frequency oscillatory ventilation suppresses inflammatory response in lung tissue and microdissected alveolar macrophages in surfactant depleted piglets. Pediatr Res 55, 339346.
41Royaee AR, Husmann RJ, Dawson HD, et al. (2004) Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet Immunol Immunopathol 102, 199216.
42Peirson SN, Butler JN & Foster RG (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31, e73.
43Gardan D, Gondret F & Louveau I (2006) Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes. Am J Physiol Endocrinol Metab 291, E372E380.
44Hartmann B, Johnsen AH, Ørskov C, et al. (2000) Structure, measurement and secretion of human glucagon-like peptide-2. Peptides 21, 7380.
45Hunt JN & Knox MT (1969) The slowing of gastric emptying by nine acids. J Physiol 201, 161179.
46Darwiche G, Ostman EM, Liljeberg HG, et al. (2001) Measurements of the gastric emptying rate by use of ultrasonography: studies in humans using bread with added sodium propionate. Am J Clin Nutr 74, 254258.
47Manzanilla EG, Perez JF, Martin M, et al. (2004) Effect of plant extracts and formic acid on the intestinal equilibrium of early-weaned pigs. J Anim Sci 82, 32103218.
48Bosi P, Mazzoni M, De Filippi S, et al. (2006) A continuous dietary supply of free calcium formate negatively affects the parietal cell population and gastric RNA expression for H+/K+-ATPase in weaning pig. J Nutr 136, 12291235.
49Castillo M, Martin-Orue SM, Roca M, et al. (2006) The response of gastrointestinal microbiota to avilamycin, butyrate, and plant extracts in early-weaned pigs. J Anim Sci 84, 27252734.
50Katoh K & Tsuda T (1987) Effects of intravenous injection of butyrate on the exocrine pancreatic secretion in guinea pigs. Comp Biochem Physiol A 87, 569572.
51Ohbo M, Katoh K & Sasaki Y (1996) Effects of saturated fatty acids on amylase release from exocrine pancreatic segments of sheep, rats, hamsters, field voles and mice. J Comp Physiol B 166, 305309.
52Sileikiene V, Mosenthin R, Tafaj M, et al. (2005) Effect of short chain fatty acids infused intraileally on interdigestive exocrine pancreatic secretions in growing pigs. J Anim Physiol Anim Nutr (Berl) 89, 253259.
53Jiang R, Chang X, Stoll B, et al. (2000) Dietary plasma protein reduces small intestinal growth and lamina propria cell density in early weaned pigs. J Nutr 130, 2126.
54Pirman T, Mosoni L, Remond D, et al. (2008) Differential response of protein metabolism in splanchnic organs and muscle to pectin feeding. Br J Nutr 100, 306311.
55Sun CQ, O'Connor CJ, Turner SJ, et al. (1998) The effect of pH on the inhibition of bacterial growth by physiological concentrations of butyric acid: implications for neonates fed on suckled milk. Chem Biol Interact 113, 117131.
56McCracken BA, Spurlock ME, Roos MA, et al. (1999) Weaning anorexia may contribute to local inflammation in the piglet small intestine. J Nutr 129, 613619.
57Pié S, Lallès JP, Blazy F, et al. (2004) Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J Nutr 134, 641647.
58Egorin MJ, Yuan ZM, Sentz DL, et al. (1999) Plasma pharmacokinetics of butyrate after intravenous administration of sodium butyrate or oral administration of tributyrin or sodium butyrate to mice and rats. Cancer Chemother Pharmacol 43, 445453.
59Sakata T (1987) Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects of fermentable fibre, gut microbes and luminal trophic factors. Br J Nutr 58, 95103.
60Inagaki A & Sakata T (2005) Dose-dependent stimulatory and inhibitory effects of luminal and serosal n-butyric acid on epithelial cell proliferation of pig distal colonic mucosa. J Nutr Sci Vitaminol 51, 156160.
61Stewart CE & Rotwein P (1996) Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors. Physiol Rev 76, 10051026.
62Weber TE & Kerr BJ (2008) Effect of sodium butyrate on growth performance and response to lipopolysaccharide in weanling pigs. J Anim Sci 86, 442450.
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British Journal of Nutrition
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