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Compared effects of three oligosaccharides on metabolism of intestinal microflora in rats inoculated with a human faecal flora

  • Zakia Djouzi (a1) and Claude Andlueux (a1)


Using germ-free rats inoculated with a human faecal flora (gnotobiotic rats), the effects of three oligosaccharides (β-fructo-oligosaccharides (FOS), β-galacto-oligosaccharides (TOS) and α-gluco-oligosaccharides (GOS)) on intestinal bacterial metabolism were compared. The animals were fed on either a control diet or diets containing 4Og/kg of GOS, FOS or TOS. FOS and TOS were the preferred growth substrates for Bifidobacteria which increased in number by 2 log values in faeces of rats when compared with rats fed on GOS or control diets. Ingestion of TOS specifically induced hydrolysis of the substrate, and did not modify the activity of any other enzymes measured in the caecum. GOS led to a non-specific enzymic induction of β-galactosidase (EC, β-glucosidase (EC and α-glucosidase (EC activities whereas β-glucuronidase (EC was lowered. Compared with the control group, FOS and TOS diets led to a significant increase in H2 and CH4 excretion; the GOS diet increased only CH4. Analysis of caecal contents revealed a decrease in pH for all diets compared with controls. Total short-chain fatty acid (SCFA) concentration increased significantly in all groups, but the SCFA profile differed between treatment groups. It was concluded that the three oligosaccharides studied had different effects which may be linked to their chemical structure. Some of these effects may be beneficial to human health



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Absolonne, J., Jossart, M., Coussement, P. & Roberfoid, M. (1995). Digestive acceptability of oligofructose. First Orafti Research Conference, Proceedings, pp. 135144. Tienen, Belgium:Orafti.
Andrieux, C., Lory, S., Dufour-Lescoat, C., de Baynast, R. & Szylit, O. (1991). Physiological effects of inulin in germ-free rats and in heteroxenic rats inoculated with a human flora. Food Hydrocolloids 5, 4956.
Beerens, H. (1990). An elective and selective isolation medium for Bifidobacterium spp. Letters in Applied Microbiology 11, 155157.
Bergman, E. N. (1990). Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiological Reviews 70, 567590.
Bond, J. H., Engel, R. R. & Levitt, M. D. (1971). Factors influencing pulmonary methane excretion in man. Journal of Experimental Medicine 133, 572588.
Bouhnik, Y., Flourié, B., Ouamé, F., Bisetti, N., Bornet, F. & Rambaud, J. C. (1994). Effets de l'administration prolongée de fructo-oligosaccharides (Actilight®) sur la flore bactérienne colique de l'homme (Effects of chronic fructo-oligosaccharides (Actilight®) ingestion on the human colonic microflora). Gastroentérologie Clinique et Biologique 18, 50A.
Debure, A., Colombel, J. F., Flourié, B., Rautureau, M. & Rambaud, J. C. (1989). Comparaison de l'implantation et de l'activité métabolique d'une flore fécale de rat et d'une flore fécale humaine inoculées chez le rat axénique (Implantation and metabolic activity of rat and human faecal bacterial flora administered to germ-free rats). Gastroentérologie Clinique et Biologique 13, 2531.
Delzenne, N., Kok, N., Fiordalso, M. F., Deboyser, D., Goethals, F. & Roberfroid, M. (1993). Dietary fructooligosaccharides modify lipid metabolism in the rat. American Journal of Clinical Nutrition 57, Suppl. 820.
De Simone, C., Vesely, R., Bianchi Salvadori, B. & Jirillo, E. (1993). Influence of long term yoghurt consumption in young adults. International Journal of Immunotherapy 7, 205210.
Djouzi, Z., Andrieux, C., Pelenc, V., Somaniba, S., Popot, F., Paul, F., Monsan, P. & Szylit, O. (1995). Degradation and fermentation of α-gluco-oligosaccharides by bacterial strains from human colon: in vitro and in vivo studies in gnotobiotic rats. Journal of Applied Bacteriology 79, 117127.
Drevon, T. & Bomet, F. (1992). Les fructo-oligosaccharides, Actilight® (Fructo-oligosaccharides, Actilight®). In Sucre, Produits Sucrants, Edulcorants et Substances Glucidiques de Charge dans les Industries Alimentaires. Techniques et Documentation. Collection Sciences et Techniques Agro-Alimentaires. pp. 314337 [Multon, J. L., editor]. Paris: Lavoisier et Apria.
Dropsy, G. & Boy, J. (1965). Détermination de l'ammonitmie (méthode automatique par dialyse) (Ammonia determination in urine). Annales de Biologie Clinique 19, 313318.
Femandes, C. F. & Shahani, K. M. (1990). Anticarcinogenic and immunological properties of dietary lactobacilli. Journal of Food Protection 53, 704710.
Gibson, G. R., Beatty, E. R., Wang, X. & Cummings, J. H. (1995). Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975982.
Gilliland, S. E. (1990). Health and nutritional benefits from lactic acid bacteria. FEMS Microbiology Reviews 87, 175188.
Goldin, B. R. & Gorbach, S. L. (1984). The effect of milk and Lactobacillus feeding on human intestinal bacterial enzyme activity. American journal of Clinical Nutrition 39, 756761.
Grimble, G. (1989). Fibre, fermentation, flora and flatus. Gut 30, 613.
Hidaka, H., Eida, T., Takizawa, T., Tokunga, T. & Tashiro, Y. (1986). Effects of fructooligosaccharides on intestinal flora and human health. Bifdobacteria Microfora 5, 3750.
Hidaka, H. & Hirayama, M. (1991). Useful characteristics and commercial applications of fructo-oligosaccharides. Biochemical Society Transactions 19, 561565.
Hitchins, A. D. & McDonough, F. E. (1989). Prophylactic and therapeutic aspects of fermented milk. American Journal of Clinical Nutrition 49, 675684.
Ito, M., Deguchi, Y., Matsumoto, K., Kimura, M., Onodera, N. & Yajima, T. (1993). Influence of galactooligosaccharides on the human faecal microflora. Journal of Nutritional Science and Vitaminology 39, 635640.
Ito, M., Deguchi, Y., Miyamori, A., Matsumoto, K., Kikuchi, H., Matsumoto, K., Kobayashi, Y., Yajima, T. & Kan, T. (1990). Effects of administration of galactooligosaccharides on the human faecal microflora, stool weight and abdominal sensation. Microbial Ecology in Health and Disease 3, 285292.
Kikuchi, H., Andrieux, C., Riottot, M., Bensaada, M., Popot, F., Beaumatin, P. & Szylit, O. (1996). Effect of two levels of transgalactosylated oligosaccharide intake in rats associated with human faecal microflora on bacterial glycolytic activity, end-products of fermentation and bacterial steroid transformation. Journal of Applied Bacteriology 80, 439446.
Kikuchi, H., Andrieux, C. & Szylit, O. (1992). Effects of galacto-oligosacharides on bacterial enzymic activities and metabolite production in rats associated with a human flora. Proceedings of the Nutrition Society 51, 7A.
Le Coz, Y., Morel, M. T., Bousseboua, H., Dufour, C. & Szylit, O. (1989). Mise au point d'une chambre respiratoire connectée sur l'isolateur pour la mesure in vivo des gaz de fermentation chez l'animal gnotoxénique (Respiratory chamber associated to isolator for measuring the in vivo production of gas in the gnotobiotic animal). Science et Technologie des Animaux de Laboratoire 14, 3539.
Lupton, J. R. (1995). Short-chain fatty acids and colon tumorigenesis: animal models. In Physiological and Clinical Aspects of Short-Chain Fatty Acids, pp. 308318 [Cummings, J. H., Rombeau, J. L. and Sakata, T., editors]. Cambridge: Cambridge University Press.
Macfarlane, G. T. & Cummings, J. H. (1991). The colonic flora, fermentation and large bowel digestive function. In The Large Intestine: Physiology, Pathophysiology and Disease, pp. 5192[ Phillips, S. F., Pemberton, J. H. and Shorter, R. G., editors]. New York: Raven Press.
Mallett, A. K., Bearne, C. A. & Rowland, I. R. (1989). The influence of incubation pH on the activity of rat and human gut flora enzymes. Journal of Applied Bacteriology 66, 433437.
Mallett, A. K., Bearne, C. A., Rowland, I. R., Farthing, M. G. J., Cole, C. B. & Fuller, R. (1987). The use of rats associated with a human faecal flora as a model for studying the effects of diet on the human gut microflora. Journal of Applied Bacteriology 63, 3945.
Mallet, A. K. & Rowland, I. R. (1988). Factors affecting the gut microflora. In Role of the Gut Flora in Toxicity and Cancer, pp. 347382. London: Academic Press.
Marteau, P., Pochart, P., Flourié, B., Pellier, P., Santos, L., Desjeux, J. F. & Rambaud, J. C. (1990). Effect of chronic ingestion of fermented dairy product containing Lactobacillus acidophilus and Bijidobacterium bijidum on metabolic activities of the colonic flora in humans. American Journal of Clinical Nutrition 52, 685688.
Matsumoto, K., Kobayashi, Y., Tamura, N., Watanabe, T. & Kan, T. (1989). Production of galactooligo-saccharides with β-galactosidase. Denpum Kagaku 36, 123130.
Mitsuoka, T. (1982). Recent trends in research on intestinal flora. Bijidobacteria Microfora 1, 324.
Mitsuoka, T. (1992). The human gastrointestinal tract. In The Lactic Acid Bacteria in Health and Disease, vol. 1, pp. 69114 [Woods, B. J. B., editor]. London and New York: Elsevier Applied Science.
Mitsuoka, T., Hidaka, H. & Eida, T. (1987). Effect of fructo-oligosaccharides on intestinal microflora. Die Nahrung 31, 427436.
Morishita, Y., Knishi, Y., Tanaka, R. & Mutai, M. (1992). The effect of transgalactosylated oligosaccharide (TOS) on the intestinal microflora and the heat resistance of fecal bacteria in rats. Bijidus 6, 1117.
Mutai, M. & Tanaka, R. (1987). Ecology of Bijdobacterium in the human intestinal flora. Bifidobacteria Microfora 6, 3341.
Nishina, P. M. & Freedland, R. A. (1990). Effects of propionate on lipid biosynthesis in isolated rat hepatocytes. Journal of Nutrition 120, 668675.
Oku, T. (1994). Special physiological functions of newly developed mono- and oligosaccharides. In Functional Foods Designer Foods, Pharmufoods, Nutraceuticals, pp. 202218 [Goldberg, I., editor]. New York: Chapman & Hall.
Roediger, W. E. W. (1980). Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa. Gut 21, 793798.
Roediger, W. E. W. (1982). Utilization of nutrients by isolated epithelial cells of rat colon. Gastroenterology 83, 424429.
Roland, N., Nugon-Baudon, L. & Rabot, S. (1993). Interactions between the intestinal flora and xenobiotic metabolizing enzymes and their health consequences. World Review of Nutrition and Dietetics 74, 123148.
Rowland, I. R. (1988). Factors affecting metabolic activity of the intestinal microflora. Drug Metabolism Reviews 19, 243261.
Rowland, I. R. & Tanaka, R. (1993). The effects of transgalactosylated oligosaccharides on gut flora metabolism in rats associated with human faecal microflora. Journal of Applied Bacteriology 74, 667674.
Rumney, C. J. & Rowland, I. R. (1992). In vivo and in vitro models of the human colonic flora. Critical Reviews in Food Science and Nutrition 31, 299331.
Saavedra, J. M., Bauman, N. A., Oung, I., Perman, J. A. & Yolken, R. H. (1994). Feeding of Bifidobacteriurn bifidum and Streptococcus thennophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet 344, 10461049.
Schriffrin, E. J., Rochat, F., Link-Amster, H., Aeschlimann, J. M. & Donnet-Hugues, A. (1995). Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. Journal of Dairy Science 78, 491497.
Segal, I., Walker, A. R. P., Lord, S. & Cummings, J. H. (1988). Breath methane and large bowel cancer risk in contrasting African populations. Gut 29, 608613.
Tamura, Z. (1983). Nutriology of Bifidobacteria. Bijidobacteria Microfora 2, 316.
Tanaka, R., Takayama, H., Morotomi, M., Kuroshima, T., Ueyama, S., Matsumoto, K., Kuroda, A. & Mutai, M. (1983). Effects of administration of TOS and Bijidobacterium breve 4006 on the human faecal flora. Bijidobacteria Microfora 2, 1724.
Tokunaga, T., Oku, T. & Hosoya, N. (1986). Influence of chronic intake of new sweetener fructo-oligosaccharide (Neosugar) on growth and gastrointestinal function of the rat. Journal of Nutritional Science and Vitaminology 32, 111121.
Trexler, P. C. (1959). The use of plastics in the design of isolator systems. Annals of the New York Academy of Sciences 78, 2936.
Uchida, K., Nomura, Y. & Takeuchi, N. (1980). Effect of cholic acid, chenodeoxycholic acid, and their related bile acids on cholesterol, phospholipid, and bile acids levels in serum, liver, bile, and faeces of rats. Journal of Biochemistry 87, 187194.
Valette, P., Pelenc, V., Djouzi, Z., Andrieux, C., Paul, F., Monsan, P. & Szylit, O. (1993). Bioavailability of new synthesised glucooligosaccharides in the intestinal tract of gnotobiotic rats. Journal of the Science of Food and Agriculture 62, 121127.
Wang, X. & Gibson, G. R. (1993). Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75, 373380.
Williams, C. H., Witherly, S. A. & Buddington, R. K. (1994). Influence of dietary neosugar on selected bacterial groups of the human faecal microbiota. Microbial Ecology in Health and Disease 7, 9197.



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