Skip to main content Accessibility help
×
Home

Dietary lysine deficiency greatly affects muscle and liver protein turnover in growing chickens*

  • S. Tesseraud (a1), R. Peresson (a1), J. Lopes (a1) and A.M. Chagneau (a1)

Abstract

We analysed the respective influences of age and lysine deficiency on skeletal muscle and liver protein turnover. Growing male broilers were fed ad libirum on isoenergetic diets containing 2OO g crude protein/kg which varied in their lysine content (7·7 or 10·1 g/kg). Fractional rates of protein synthesis (FSR) were measured in vivo in the liver and the pectoralis major muscle of 2-, 3- and 4-week-old chickens (flooding dose of l-[143H]phenylalanine). Fractional rates of proteolysis (FBR) were estimated for the same tissues as the difference between synthesis and growth. Over the 2-week period liver FSR and FBR were unchanged, whereas muscle FSR decreased with age. This developmental decline was related to the lower capacity for protein synthesis (Cs) without any modifications of the translational efficiency. Whatever the age, lysine deficiency resulted in significant decreases in body weight, tissue protein content and tissue protein deposition, apparently because of reduced amounts of proteins synthesized. We recorded a difference in the response of the two tissues to lysine deficiency, the pectoralis major being more sensitive than the liver. When comparing birds of the same age, liver FSR and FBR were not modified by the diet, where as muscle FSR, Cs and FBR were higher in chicks fed on a lysinc-deficient diet than in the controls. Conversely, when chicks of similar weights were compared, the main effect of the dietary deficiency was an increase in muscle FBR. The results suggest that lysine deficiency not only delayed chick development so that protein turnover was affected, but also induced greater changes in metabolism. Thus, the principal mechanism whereby muscle mass decreased appeared to be a change in FBR.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dietary lysine deficiency greatly affects muscle and liver protein turnover in growing chickens*
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Dietary lysine deficiency greatly affects muscle and liver protein turnover in growing chickens*
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Dietary lysine deficiency greatly affects muscle and liver protein turnover in growing chickens*
      Available formats
      ×

Copyright

References

Hide All
Akinwande, A. I. &Bragg, D. B. (1985). Effect of the level ofdietary lysine on turnover rate of liver protein in the chick. Poultry Science 64, 19381940.
Arnal, M., Obled, C., Attaix, D.Patureau, M.P. & Bonin, D. (1987).Dietary control ofprotein turnover. Diabere Metabolisme 13, 630642.
Attaix, D., Aurousseau, E., Bayle, G., Rosolowska-Huszcz, D. &Arnal, M. (1988). Respective influence of age and weaning on skeletal and visceral muscle protein synthesis in the lamb. Biochemical Journal 256, 791795.
Conway, J.M., Bier, D.M., Motil, K.J., Burke, J.F. &Young, V.R. (1980). Whole-body lysine flux in young adult meneffects of reduced total protein and of lysine intake. American Journal of Physiology 239, E192–E200.
Fuller, M. F., Reeds, P. J., Cadenhead, A., Seve, B. & Preston, T. (1987). Effect of the amount and quality of dietary protein on nitrogen metabolism and protein turnover of pigs. British Journal of Nutrition 58, 287300
Garlick, P.J., McNurlanM.A, M.A,, Essen, P. & Werneman, J. (1994). Measurement of tissue protein synthesis rates in vivo: critical analysis of contrasting methods. American Journal of Physiology 266, E287–E297.
Garlick, P. J., McNurlan, M. A. &Preedy, V.R. (1980). A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]-Phe. Biochemical Journal 192, 719723.
Goldspink, D. F. &Kelly, F.J. (1984). Protein turnover and growth in the whole-body, liver and kidney of the rat from the foetus to the senility. Biochemical Journal 217, 507516.
Griffin, H.D. & Goddard, C. (1994). Rapidly growing broiler (meat-type) chickens: their originand use for comparative studies of the regulation of growth. International Journal of Biochemistry 26, 1928.
Kino, K. & Okumura, J. (1986 a). The effect of single essential amino acid deprivation on chick growth and nitrogen and energy balances at ad libitum and equalized-food intakes. Poultry Science 65, 17281735.
Kino, K. & Okumura, J. (1986 b). Improvement of body weight and nitrogen balance of chicks fed histidine-free or lysine-free diets with supplementation of graded levels of sulfurcontainingamino acids. Poultry Science 65,17361740.
Kino, K. &Okumura, J. (1987). Whole-body protein turnover in chicks fed control, histidine, or methionine plus cystine-free diets. Poultry Science 66, 13921397.
Lobley, G.E. (1993). Species comparisons of tissue protein metabolism effects of age and hormonal action. Journal of Nutrition 123, 337343.
McDonald, M. L. & Swick, R. W. (1981). The effect of protein depletion and repletion on muscle-protein turnover in the chick. Biochemical Journal 194, 811819.
McNurlan, M. A. & Garlick, P.J. (1989). Influence of nutrient intake on protein turnover. Diabetes/Metabolism Reviews 5, 165189.
McNurlan, M.A., Tomkins, A. M. & Garlick, P.J. (1979). The effect of starvation on the rate of protein synthesis in rat liver and small intestine. Biochemical Journal 178, 375379.
Maruyama, K., Sunde, M.L. & Swick, R.W. (1978). Growth and muscle protein turnover in the chick. Biochemical Journal 176, 573582.
Meredith, C. N., Wen, Z. M., Bier, D. M., Matthews, D. E. & Young, V.R. (1986). Lysine kinetics at graded lysine intakes in young men. American Journal of Clinical Nutrition 43,787794.
Mosoni, L., Valluy, M.C., Sermrier, B., PrugnaudJ., J.,Obled, C., Guezmnec, C.Y. & Patureau, Mirand P. (1995). Altered response of protein synthesis to nutritional state and endurance training in old rats. American Journal of Physiology 268, E328–E335.
Munro, H. N. & Fleck, A. (1969). Analysis of tissues and body fluids for nitrogenous constituents. In Mammalian Protein Metabolism vol. 3, pp. 424525 [ Munro, H.N., editor] New York: Academic Press.
Muramatsu, T. (1990). Nutrition and whole-body protein turnover in the chicken in relation to mammalian species. Nutrition Research Reviews 3, 211228.
Muramatsu, T., Muramatsu, S., Okumura, J. &Tasaki, I. (1987). Developmental fall in whole body protein turnover of chick embryos during incubation. British Poultry Science 28, 165172.
Muramatsu, T. &Okumura, J. (1985). Whole-body protein turnover in chicks at early stages of growth. Journal of Nutrition 115, 483490.
Nieto, R., Palmer, R. M., Fernandez-Figares, I., Perez, L. & Prieto, C. (1994). Effect of dietary protein quality, feed restriction and short-term fasting on protein synthesis and turnover in tissues of the growing chicken. British Journal of Nutrition 72, 499507.
Okumura, J., Mori, S. &Muramatsu, T. (1985). Relationship between food consumption and energy and nitrogen utilisation by chicks given varying amounts of standard and leucine-, isoleucine- and valine-deficient diets. British Poultry Science 26, 519525.
Salter, D. N., Montgomery, A. I., Hudson, A., Quelch, D. B. & Elliot, R. J. (1990). Lysine requirements and wholebody protein turnover in growing pigs. British Journal of Nutrition 63, 503513
Smith, P.K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. & Klenk, D. C. (1985). Measurement of protein using bicinchoninic acid. Analytical Biochemistry 150, 7685.
Suzuki, O. &Yagi, K. (1976). A fluorometric assay for phenylethylamine in rat brain. Analytical Biochemistry 75, 192200.
Tesseraud, S., Cammas, J. C. & Chapeau, A. M. (1994). Effect of age and divergent selection for body weight on muscle protein turnover in chickens. Reproduction Nutrition Development 34, 638639.
Tesseraud, S., Larbier, M., Chapeau, A. M. &Geraert, P. A. (1992). Effects of dietary lysine on muscle protein turnover in growing chickens. Reproduction Nutrition Development 32, 163175.
Tomas, F. M., Pym, R. A. &Johnson, R.J. (1991). Muscle protein turnover in chickens selected for increased growth rate, food consumption or efficiency of food utilisation effects of genotype and relationship to plasma IGF-I and growth hormone. British Poultry Science 32, 363376.
Vary, T.C. &Kimball, S.R. (1992 a). Regulation of hepatic protein synthesis in chronic inflammation and sepsis. American Journal of Physiology 262, C445C452.
Vary, T.C. & Kimball, S.R. (1992 b). Sepsis-induced changes in protein synthesis differential effects on fast- and slow-twitch muscles. American Journal of Physiology 262, C1513C1519.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed