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Dietary protein requirements and adaptive advantages in athletes

  • Stuart M. Phillips (a1)

Dietary guidelines from a variety of sources are generally congruent that an adequate dietary protein intake for persons over the age of 19 is between 0·8–0·9 g protein/kg body weight/d. According to the US/Canadian Dietary Reference Intakes, the RDA for protein of 0·8 g protein/kg/d is “…the average daily intake level that is sufficient to meet the nutrient requirement of nearly all [~98 %]… healthy individuals…” The panel also states that “…no additional dietary protein is suggested for healthy adults undertaking resistance or endurance exercise.” These recommendations are in contrast to recommendations from the US and Canadian Dietetic Association: “Protein recommendations for endurance and strength trained athletes range from 1·2 to 1·7 g/kg/d.” The disparity between those setting dietary protein requirements and those who might be considered to be making practical recommendations for athletes is substantial. This may reflect a situation where an adaptive advantage of protein intakes higher than recommended protein requirements exists. That population protein requirements are still based on nitrogen balance may also be a point of contention since achieving balanced nitrogen intake and excretion likely means little to an athlete who has the primary goal of exercise performance. The goal of the present review is to critically analyse evidence from both acute and chronic dietary protein-based studies in which athletic performance, or correlates thereof, have been measured. An attempt will be made to distinguish between protein requirements set by data from nitrogen balance studies, and a potential adaptive ‘advantage’ for athletes of dietary protein in excess of the RDA.

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* S. M. Phillips, fax +905-523-6011, email,
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1Institute of Medicine (2005) Dietary Reference Intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids. Washington, DC: National Academies Press.
2Rodriguez NR, Di Marco NM & Langley S (2009) American College of Sports Medicine position stand. Nutrition and athletic performance. Med Sci Sports Exerc 41, 709731.
3WHO Technical Report Series 935. Protein and Amino Acid Requirements in Human Nutrition: report of a joint FAO/WHO/UNU expert consultation. Report of a JointWHO/FAO/UNU Expert Consultation. 2011.
4Phillips SM, Hartman JW & Wilkinson SB (2005) Dietary protein to support anabolism with resistance exercise in young men. J Am Coll Nutr 24, 134S139S.
5Phillips SM (2004) Protein requirements and supplementation in strength sports. Nutrition 20, 689695.
6Phillips SM (2006) Dietary protein for athletes: from requirements to metabolic advantage. App Physiol Nutr Metab 31, 647654.
7Tarnopolsky MA (2004) Protein requirements for endurance athletes. Nutrition 20, 662668.
8Rennie MJ & Tipton KD (2000) Protein and amino acid metabolism during and after exercise and the effects of nutrition. Annu Rev Nutr 20, 457483.
9Burke LM (2001) Energy needs of athletes. Can J Appl Physiol 26, S202S219.
10Burke LM, Cox GR, Culmmings NK, et al. (2001) Guidelines for daily carbohydrate intake: do athletes achieve them? Sports Med 31, 267299.
11Burke LM, Kiens B & Ivy JL (2004) Carbohydrates and fat for training and recovery. J Sports Sci 22, 1530.
12Burke LM, Millet G & Tarnopolsky MA (2007) Nutrition for distance events. J Sports Sci 25, Suppl. 1, S29S38.
13Biolo G, Ciocchi B, Stulle M, et al. (2007) Calorie restriction accelerates the catabolism of lean body mass during 2 wk of bed rest. Am J Clin Nutr 86, 366372.
14Biolo G, Agostini F, Simunic B, et al. (2008) Positive energy balance is associated with accelerated muscle atrophy and increased erythrocyte glutathione turnover during 5 wk of bed rest. Am J Clin Nutr 88, 950958.
15Astrup A, Dyerberg J, Elwood P, et al. (2011) The role of reducing intakes of saturated fat in the prevention of cardiovascular disease: where does the evidence stand in 2010? Am J Clin Nutr 93, 684688.
16Rand WM, Pellett PL & Young VR (2003) Meta-analysis of nitrogen balance studies for estimating protein requirements in healthy adults. Am J Clin Nutr 77, 109127.
17Millward DJ & Rivers JP (1988) The nutritional role of indispensable amino acids and the metabolic basis for their requirements. Eur J Clin Nutr 42, 367393.
18Millward DJ & Rivers JP (1989) The need for indispensable amino acids: the concept of the anabolic drive. Diabetes Metab Rev 5, 191211.
19Babraj J, Cuthbertson DJ, Rickhuss P, et al. (2002) Sequential extracts of human bone show differing collagen synthetic rates. Biochem Soc Trans 30, 6165.
20Babraj JA, Smith K, Cuthbertson DJ, et al. (2005) Human bone collagen synthesis is a rapid, nutritionally modulated process. J Bone Miner Res 20, 930937.
21Heinemeier K, Langberg H, Olesen JL, et al. (2003) Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue. J Appl Physiol 95, 23902397.
22Rennie MJ, Wackerhage H, Spangenburg EE, et al. (2004) Control of the size of the human muscle mass. Annu Rev Physiol 66, 799828.
23Nakshabendi IM, McKee R, Downie S, et al. (1999) Rates of small intestinal mucosal protein synthesis in human jejunum and ileum. Am J Physiol 277, E1028E1031.
24Nakshabendi IM, Obeidat W, Russell RI, et al. (1995) Gut mucosal protein synthesis measured using intravenous and intragastric delivery of stable tracer amino acids. Am J Physiol 269, E996E999.
25Young VR (1986) Nutritional balance studies: indicators of human requirements or of adaptive mechanisms? J Nutr 116, 700703.
26Young VR, Wayler A, Garza C, et al. (1984) A long-term metabolic balance study in young men to assess the nutritional quality of an isolated soy protein and beef proteins. Am J Clin Nutr 39, 815.
27McKenzie S, Phillips SM, Carter SL, et al. (2000) Endurance exercise training attenuates leucine oxidation and BCOAD activation during exercise in humans. Am J Physiol Endocrinol Metab 278, E580E587.
28Phillips SM, Atkinson SA, Tarnopolsky MA, et al. (1993) Gender differences in leucine kinetics and nitrogen balance in endurance athletes. J Appl Physiol 75, 21342141.
29Lamont LS, McCullough AJ & Kalhan SC (1999) Comparison of leucine kinetics in endurance-trained and sedentary humans. J Appl Physiol 86, 320325.
30Lamont LS, McCullough AJ & Kalhan SC (2001) Relationship between leucine oxidation and oxygen consumption during steady-state exercise. Med Sci Sports Exerc 33, 237241.
31Lamont LS, McCullough AJ & Kalhan SC (2001) Gender differences in leucine, but not lysine, kinetics. J Appl Physiol 91, 357362.
32Miller BF, Olesen JL, Hansen M, et al. (2005) Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol 567, 10211033.
33Sheffield-Moore M, Yeckel CW, Volpi E, et al. (2004) Post exercise protein metabolism in older and younger men following moderate-intensity aerobic exercise. Am J Physiol Endocrinol Metab 287, E513E522.
34Carraro F, Stuart CA, Hartl WH, et al. (1990) Effect of exercise and recovery on muscle protein synthesis in human subjects. Am J Physiol 259, E470E476.
35Hartman JW, Moore DR & Phillips SM (2006) Resistance training reduces whole-body protein turnover and improves net protein retention in untrained young males. Appl Physiol Nutr Metab 31, 557564.
36Moore DR, Del Bel NC, Nizi KI, et al. (2007) Resistance training reduces fasted- and fed-state leucine turnover and increases dietary nitrogen retention in previously untrained young men. J Nutr 137, 985991.
37Butterfield GE & Calloway DH (1984) Physical activity improves protein utilization in young men. Br J Nutr 51, 171184.
38Todd KS, Butterfield GE & Calloway DH (1984) Nitrogen balance in men with adequate and deficient energy intake at three levels of work. J Nutr 114, 21072118.
39Phillips SM, Tipton KD, Aarsland A, et al. (1997) Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol 273, E99E107.
40Millward DJ & Jackson AA (2004) Protein/energy ratios of current diets in developed and developing countries compared with a safe protein/energy ratio: implications for recommended protein and amino acid intakes. Public Health Nutr 7, 387405.
41Burd NA, Tang JE, Moore DR, et al. (2009) Exercise training and protein metabolism: influences of contraction, protein intake, and sex-based differences. J Appl Physiol 106, 16921701.
42Miller SL, Tipton KD, Chinkes DL, et al. (2003) Independent and combined effects of amino acids and glucose after resistance exercise. Med Sci Sports Exerc 35, 449455.
43Rasmussen BB, Tipton KD, Miller SL, et al. (2000) An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise. J Appl Physiol 88, 386392.
44Tipton KD, Ferrando AA, Phillips SM, et al. (1999) Postexercise net protein synthesis in human muscle from orally administered amino acids. Am J Physiol 276, E628E634.
45Tipton KD, Rasmussen BB, Miller SL, et al. (2001) Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Am J Physiol Endocrinol Metab 281, E197E206.
46Tipton KD, Elliott TA, Cree MG, et al. (2004) Ingestion of casein and whey proteins result in muscle anabolism after resistance exercise. Med Sci Sports Exerc 36, 20732081.
47Moore DR, Robinson MJ, Fry JL, et al. (2009) Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr 89, 161168.
48Tipton KD & Wolfe RR (2001) Exercise, protein metabolism, and muscle growth. Int J Sport Nutr Exerc Metab 11, 109132.
49Wolfe RR (2000) Protein supplements and exercise. Am J Clin Nutr 72, 551S557S.
50Friedman JE & Lemon PW (1989) Effect of chronic endurance exercise on retention of dietary protein. Int J Sports Med 10, 118123.
51Reeds PJ & Garlick PJ (2003) Protein and amino acid requirements and the composition of complementary foods. J Nutr 133, 2953S2961S.
52Anthony JC, Anthony TG, Kimball SR, et al. (2001) Signaling pathways involved in translational control of protein synthesis in skeletal muscle by leucine. J Nutr 131, 856S860S.
53Anthony JC, Yoshizawa F, Anthony TG, et al. (2000) Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathway. J Nutr 130, 24132419.
54Anthony JC, Reiter AK, Anthony TG, et al. (2002) Orally administered leucine enhances protein synthesis in skeletal muscle of diabetic rats in the absence of increases in 4E-BP1 or S6K1 phosphorylation. Diabetes 51, 928936.
55Bolster DR, Pikosky MA, Gaine PC, et al. (2005) Dietary protein intake impacts human skeletal muscle protein fractional synthetic rates after endurance exercise. Am J Physiol Endocrinol Metab 289, E678E683.
56Cuthbertson D, Smith K, Babraj J, et al. (2005) Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J 19, 422424.
57Tipton KD, Gurkin BE, Matin S, et al. (1999) Non essential amino acids are not necessary to stimulate net muscle protein synthesis in healthy volunteers. J Nutr Biochem 10, 8995.
58Volpi E, Kobayashi H, Sheffield-Moore M, et al. (2003) Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Am J Clin Nutr 78, 250258.
59Moore DR, Tang JE, Burd NA, et al. (2009) Differential stimulation of myofibrillar and sarcoplasmic protein synthesis with protein ingestion at rest and after resistance exercise. J Physiol 597, 897904.
60Bowtell JL, Leese GP, Smith K, et al. (1998) Modulation of whole body protein metabolism, during and after exercise, by variation of dietary protein. J Appl Physiol 85, 17441752.
61Millward DJ (2004) Macronutrient intakes as determinants of dietary protein and amino acid adequacy. J Nutr 134, 1588S1596S.
62Millward DJ (1998) Metabolic demands for amino acids and the human dietary requirement: Millward and Rvers (1988) revisited. J Nutr 128, 2563S2576S.
63Quevedo MR, Price GM, Halliday D, et al. (1994) Nitrogen homoeostasis in man: diurnal changes in nitrogen excretion, leucine oxidation and whole body leucine kinetics during a reduction from a high to a moderate protein intake. Clin Sci (Lond) 86, 185193.
64Price GM, Halliday D, Pacy PJ, et al. (1994) Nitrogen homeostasis in man: influence of protein intake on the amplitude of diurnal cycling of body nitrogen. Clin Sci (Lond) 86, 91102.
65Deuster PA, Kyle SB, Moser PB, et al. (1986) Nutritional survey of highly trained women runners. Am J Clin Nutr 44, 954962.
66Singh A, Deuster PA, Day BA, et al. (1990) Dietary intakes and biochemical markers of selected minerals: comparison of highly trained runners and untrained women. J Am Coll Nutr 9, 6575.
67Snead DB, Stubbs CC, Weltman JY, et al. (1992) Dietary patterns, eating behaviors, and bone mineral density in women runners. Am J Clin Nutr 56, 705711.
68Beals KA & Manore MM (1998) Nutritional status of female athletes with subclinical eating disorders. J Am Diet Assoc 98, 419425.
69MacDougall JD, Ray S, Sale DG, et al. (1999) Muscle substrate utilization and lactate production. Can J Appl Physiol 24, 209215.
70Lambert CP & Flynn MG (2002) Fatigue during high-intensity intermittent exercise: application to bodybuilding. Sports Med 32, 511522.
71Robergs RA, Pearson DR, Costill DL, et al. (1991) Muscle glycogenolysis during differing intensities of weight-resistance exercise. J Appl Physiol 70, 17001706.
72Essen-Gustavsson B & Tesch PA (1990) Glycogen and triglyceride utilization in relation to muscle metabolic characteristics in men performing heavy-resistance exercise. Eur J Appl Physiol Occup Physiol 61, 510.
73Tesch PA, Colliander EB & Kaiser P (1986) Muscle metabolism during intense, heavy-resistance exercise. Eur J Appl Physiol Occup Physiol 55, 362366.
74Haff GG, Lehmkuhl MJ, McCoy LB, et al. (2003) Carbohydrate supplementation and resistance training. J Strength Cond Res 17, 187196.
75Kuhnlein HV & Receveur O (2007) Local cultural animal food contributes high levels of nutrients for Arctic Canadian Indigenous adults and children. J Nutr 137, 11101114.
76Kuhnlein HV, Soueida R & Receveur O (1996) Dietary nutrient profiles of Canadian Baffin Island Inuit differ by food source, season, and age. J Am Diet Assoc 96, 155162.
77Risica PM, Nobmann ED, Caulfield LE, et al. (2005) Springtime macronutrient intake of Alaska natives of the Bering Straits Region: the Alaska Siberia Project. Int J Circumpolar Health 64, 222233.
78Nobmann ED, Byers T, Lanier AP, et al. (1992) The diet of Alaska Native adults: 1987-1988. Am J Clin Nutr 55, 10241032.
79Feskanich D, Willett WC, Stampfer MJ, et al. (1996) Protein consumption and bone fractures in women. Am J Epidemiol 143, 472479.
80Wengreen HJ, Munger RG, West NA, et al. (2004) Dietary protein intake and risk of osteoporotic hip fracture in elderly residents of Utah. J Bone Miner Res 19, 537545.
81Munger RG, Cerhan JR & Chiu BC (1999) Prospective study of dietary protein intake and risk of hip fracture in postmenopausal women. Am J Clin Nutr 69, 147152.
82Bonjour JP (2005) Dietary protein: an essential nutrient for bone health. J Am Coll Nutr 24, 526S536S.
83Bamman MM, Shipp JR, Jiang J, et al. (2001) Mechanical load increases muscle IGF-I and androgen receptor mRNA concentrations in humans. Am J Physiol Endocrinol Metab 280, E383E390.
84Hameed M, Orrell RW, Cobbold M, et al. (2003) Expression of IGF-I splice variants in young and old human skeletal muscle after high resistance exercise. J Physiol 547, 247254.
85Nindl BC, Kraemer WJ, Marx JO, et al. (2001) Overnight responses of the circulating IGF-I system after acute, heavy-resistance exercise. J Appl Physiol 90, 13191326.
86Layman DK, Boileau RA, Erickson DJ, et al. (2003) A reduced ratio of dietary carbohydrate to protein improves body composition and blood lipid profiles during weight loss in adult women. J Nutr 133, 411417.
87Layman DK, Shiue H, Sather C, et al. (2003) Increased dietary protein modifies glucose and insulin homeostasis in adult women during weight loss. J Nutr 133, 405410.
88Zemel MB (2003) Mechanisms of dairy modulation of adiposity. J Nutr 133, 252S256S.
89Zemel MB, Thompson W, Milstead A, et al. (2004) Calcium and dairy acceleration of weight and fat loss during energy restriction in obese adults. Obes Res 12, 582590.
90Pins JJ & Keenan JM (2006) Effects of whey peptides on cardiovascular disease risk factors. J Clin Hypertens (Greenwich) 8, 775782.
91Bernstein AM, Treyzon L & Li Z (2007) Are high-protein, vegetable-based diets safe for kidney function? A review of the literature. J Am Diet Assoc 107, 644650.
92Richardson DP, Wayler AH, Scrimshaw NS, et al. (1979) Quantitative effect of an isoenergetic exchange of fat for carbohydrate on dietary protein utilization in healthy young men. Am J Clin Nutr 32, 22172226.
93Borsheim E, Cree MG, Tipton KD, et al. (2004) Effect of carbohydrate intake on net muscle protein synthesis during recovery from resistance exercise. J Appl Physiol 96, 674678.
94Chow LS, Albright RC, Bigelow ML, et al. (2006) Mechanism of insulin's anabolic effect on muscle: measurements of muscle protein synthesis and breakdown using aminoacyl-tRNA and other surrogate measures. Am J Physiol Endocrinol Metab 291, E729E736.
95Krieger JW, Sitren HS, Daniels MJ, et al. (2006) Effects of variation in protein and carbohydrate intake on body mass and composition during energy restriction: a meta-regression. Am J Clin Nutr 83, 260274.
96Layman DK, Evans E, Baum JI, et al. (2005) Dietary protein and exercise have additive effects on body composition during weight loss in adult women. J Nutr 135, 19031910.
97Phillips SM, Tipton KD, Ferrando AA, et al. (1999) Resistance training reduces the acute exercise-induced increase in muscle protein turnover. Am J Physiol 276, E118E124.
98Mettler S, Mitchell N & Tipton KD (2010) Increased protein intake reduces lean body mass loss during weight loss in athletes. Med Sci Sports Exerc 42, 326337.
99Pikosky MA, Smith TJ, Grediagin A, et al. (2008) Increased protein maintains nitrogen balance during exercise-induced energy deficit. Med Sci Sports Exerc 40, 505512.
100Burke LM, Kiens B & Ivy JL (2004) Carbohydrates and fat for training and recovery. J Sports Sci 22, 1530.
101Holm L, Esmarck B, Mizuno M, et al. (2006) The effect of protein and carbohydrate supplementation on strength training outcome of rehabilitation in ACL patients. J Orthop Res 24, 21142123.
102Andersen LL, Tufekovic G, Zebis MK, et al. (2005) The effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength. Metabolism 54, 151156.
103Esmarck B, Andersen JL, Olsen S, et al. (2001) Timing of postexercise protein intake is important for muscle hypertrophy with resistance training in elderly humans. J Physiol 535, 301311.
104Hartman JW, Tang JE, Wilkinson SB, et al. (2007) Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86, 373381.
105Cribb PJ & Hayes A (2006) Effects of supplement timing and resistance exercise on skeletal muscle hypertrophy. Med Sci Sports Exerc 38, 19181925.
106Josse AR, Tang JE, Tarnopolsky MA, et al. (2010) Body composition and strength changes in women with milk and resistance exercise. Med Sci Sports Exerc 42, 11221130.
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