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Differential responses to selenomethionine supplementation by sex and genotype in healthy adults

  • Gerald F. Combs (a1), Matthew I. Jackson (a1), Jennifer C. Watts (a1), LuAnn K. Johnson (a1), Huawei Zeng (a1), Joseph Idso (a1), Lutz Schomburg (a2), Antonia Hoeg (a2), Carolin S. Hoefig (a2), Emily C. Chiang (a3), David J. Waters (a3), Cindy D. Davis (a4) and John A. Milner (a4)...
Abstract

A year-long intervention trial was conducted to characterise the responses of multiple biomarkers of Se status in healthy American adults to supplemental selenomethionine (SeMet) and to identify factors affecting those responses. A total of 261 men and women were randomised to four doses of Se (0, 50, 100 or 200 μg/d as l-SeMet) for 12 months. Responses of several biomarkers of Se status (plasma Se, serum selenoprotein P (SEPP1), plasma glutathione peroxidase activity (GPX3), buccal cell Se, urinary Se) were determined relative to genotype of four selenoproteins (GPX1, GPX3, SEPP1, selenoprotein 15), dietary Se intake and parameters of single-carbon metabolism. Results showed that supplemental SeMet did not affect GPX3 activity or SEPP1 concentration, but produced significant, dose-dependent increases in the Se contents of plasma, urine and buccal cells, each of which plateaued by 9–12 months and was linearly related to effective Se dose (μg/d per kg0·75). The increase in urinary Se excretion was greater for women than men, and for individuals of the GPX1 679 T/T genotype than for those of the GPX1 679 C/C genotype. It is concluded that the most responsive Se-biomarkers in this non-deficient cohort were those related to body Se pools: plasma, buccal cell and urinary Se concentrations. Changes in plasma Se resulted from increases in its non-specific component and were affected by both sex and GPX1 genotype. In a cohort of relatively high Se status, the Se intake (as SeMet) required to support plasma Se concentration at a target level (Sepl-target) is: .

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Corresponding author
*Corresponding author: Dr G. F. Combs, fax +1 701 795 8230, email gerald.combs@ars.usda.gov
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2 S Toppo , L Flohé , F Ursini , (2009) Catalytic mechanisms and specificities of glutathione peroxidases: variations of a basic scheme. Biochim Biophys Acta 1790, 14861500.

3 LH Duntas (2010) Selenium and the thyroid: a close-knit connection. J Clin Endocrinol Metab 95, 51805188.

7 LC Clark , GF Combs Jr, BW Turnbull , (1996) Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. A randomized controlled trial. Nutritional Prevention of Cancer Study Group. J Am Med Assoc 276, 19571963.

8 AJ Duffield-Lillico , BL Dalkin , ME Reid , (2003) Selenium supplementation, baseline plasma selenium status and incidence of prostate cancer: an analysis of the complete treatment period of the Nutritional Prevention of Cancer Trial. Br J Urol Int 91, 608612.

9 S Stranges , JR Marshall , R Natarajan , (2007) Effects of long-term selenium supplementation on type 2 diabetes: A randomized trial. Ann Int Med 147, 217223.

10 J Bleys , A Navas-Acien & E Gualler (2007) Serum selenium and diabetes in U.S. adults. Diabetes Care 30, 829834.

12 SM Lipmann , EA Klein , PJ Goodman , (2009) Effect of selenium and vitamin E on risk of prostate and other cancers. J Am Med Assoc 301, 3951.

16 RF Burk , BK Norsworthy , KE Hill , (2006) Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial. Cancer Epidemiol Biomarkers Prev 15, 804810.

22 GF Combs Jr, JC Watts , MI Jackson , (2011) Determinants of selenium status in healthy adults. Nutr J 10, 7582.

29 RA Lawrence & RF Burk (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71, 952958.

32 Y Saito , N Sato , M Hirashima , (2004) Domain structure of bi-functional selenoprotein P. Biochem J 381, 841846.

33 S Kokarnig , D Kuehnelt , M Stiboller , (2011) Quantitative determination of small selenium species in human serum by HPLC/ICPMS following a protein-removal, pre-concentration procedure. Anal Bioanal Chem 400, 23232327.

34 DJ Waters , S Shen , LT Glickman , (2005) Prostate cancer risk and DNA damage: translational significance of selenium supplementation in a canine model. Carcinogenesis 26, 12561262.

35 SJ Duthie & AR Collins (1997) The influence of cell growth, detoxifying enzymes and DNA repair on hydrogen peroxide-mediated DNA damage (measured using the comet assay) in human cells. Free Radic Biol Med 22, 717724.

36 L Schomburg & U Schweizer (2009) Hierarchical regulation of selenoprotein expression and sex-specific effects of selenium. Biochim Biophys Acta 1790, 14531462.

37 C Méplan , LK Crosley , F Nicol , (2007) Genetic polymorphisms in the human selenoprotein P gene determine the response of selenoprotein markers to selenium supplementation in a gender-specific manner (the SELGEN study). FASEB J 21, 30633074.

39 M Stoedter , K Renko , A Hög , (2010) Selenium controls the sex-specific immune response and selenoprotein expression during the acute-phase response in mice. Biochem J 429, 4351.

43 C Cominetti , MC de Bartoli , E Prgatto , (2011) Associations between glutathione peroxidase-1 Pro198Leu polymorphism, selenium status, and DNA damage in obese women after consumption of Brazil nuts. Nutrition 27, 891896.

49 CW Hawkes , NL Keim , D Richter , (2008) High-selenium yeast supplementation in free-living North American men: no effect on thyroid hormone metabolism or body composition. J Trace Elem Med Biol 22, 131142.

50 J Lu , M Kaeck , C Jiang , (1994) Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells. Biochem Pharmacol 47, 15311535.

54 OE Olson , E Novachek , E Whitehead , (1970) Investigations on selenium in wheat. Phytochemistry 9, 11811188.

55 D Thavarajah , A Vandenberg , GN George , (2007) Chemical form of selenium in naturally selenium-rich lentils (Lens culinaris L.) from Saskatchewan. J Agric Food Chem 55, 73377341.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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