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The digestion by cattle of silage-containing diets fed at two dry matter intakes

*2. Digestion of total amino acids and of D-alanine and D-glutamic acid

Published online by Cambridge University Press:  09 March 2007

H. A. Greife ,
J. A. Rooke  and
D. G. Armstrong
H. A. Greife
Affiliation:
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, Newcastle upon TyneNEI 7RU
J. A. Rooke
Affiliation:
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, Newcastle upon TyneNEI 7RU
D. G. Armstrong
Affiliation:
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, Newcastle upon TyneNEI 7RU
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Abstract

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1. In a 4 x 4 Latin square experiment four cows were given, twice daily, diets consisting of (g/kg dry matter (DM)) 500 barley, 400 grass silage and 100 soya-bean meal. The diets were given at either 1.15 (L) or 2.3 (H) times maintenance energy requirements and the soya-bean meal was either untreated (U) or formaldehyde (HCH0)-treated (T).

2. The passage of digesta to the duodenum was estimated using chromic oxide as a flow marker; 35S was used to estimate the amount of microbial protein entering the small intestine. A microbial fraction was prepared by differential centrifugation from duodenal digesta. Samples of bacteria and of protozoa from rumen digesta were also prepared.

3. The total amino acid contents of feedingstuffs, duodenal digesta, duodenal microbial material, rumen bacteria and rumen protozoa were determined by ion-exchange chromatography. The D-alanine and D-glutamic acid contents of the samples were determined by gas–liquid chromatography.

4. The quantity of each amino acid entering the small intestine was significantly (P < 0,001) increased by increasing DM intake and tended to be increased by formaldehyde-treatment of the soya-bean meal. There were net losses of all amino acids across the forestomachs except for lysine, methione, o-alanine and D-glutamic acid for which there were net gains.

5. There were significant (P < 0.05) differences in amino acid composition between rumen bacteria and duodenal microbial material; differences in amino acid composition between rumen bacteria and rumen protozoa were also observed.

6. D-Alanine and D-glutamic acid were present in the silage but not in the barley or either of the soya-bean meals. All samples of microbes and digesta contained D-alanine and D-glutamic acid.

7. The use of D-ahine and D-glUtamiC acid as markers for microbial nitrogen entering the small intestine was assessed. Estimates of the quantities of microbial N entering the small intestine based on the D-alanine or D-glutamic acid contents of rumen bacteria or duodenal microbes were significantly higher than those determined using 35S as a marker.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 54 , Issue 2 , September 1985 , pp. 483 - 492
Copyright
Copyright © The Nutrition Society 1985

References

REFERENCES

Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Agricultural Research Council (1984). The Nutrient Requirements of Ruminant Livestock, Suppl No. 1. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Brown, C. M. & Stanley, S. O. (1972). Journal of Applied Chemistry and Biotechnology 22, 363–372.Google Scholar
Buttery, P. J. (1982). In Forage Protein in Ruminant Animal Production, Occasional Publication, British Society of Animal Production, no. 6, pp.1–12 [Thomson, D. J., Beever, D. E. and Gunn, R. G. Editors]. Thames Ditton: British Society of Animal Production.Google Scholar
Coleman, G. S. (1975). In Digestion and Metabolism in the Rumen, pp. 149–164 [McDonald, I. W. and Warner, A. C. L. editors]. Armidale: University of New England Publishing Unit.Google ScholarPubMed
Czerkawski, J. W. (1976). Journal of the Science of Food and Agriculture 27, 621–632.CrossRefGoogle Scholar
Dehority, B. A. & Grubb, J. A. (1980). Applied and Environmental Microbiology 39, 376–381.CrossRefGoogle Scholar
Frank, H., Nicholson, G. J. & Bayer, E. (1978). Journal of Chromatography 167, 187–196.CrossRefGoogle Scholar
Garrett, J. E., Goodrich, R. D. & Meiske, J. C. (1982). In Protein Requirements for Cattle: Symposium, pp. 23–25 [Owens, F. N., editor]. Oklahoma: Oklahoma State University.Google Scholar
Greife, H. A., Rooke, J. A. & Armstrong, D. G. (1983). Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 50, 36–37.Google Scholar
Harrison, D. G., Beever, D. E. & Osbourn, D. F. (1979). British Journal of Nutrition 41, 521–527.CrossRefGoogle Scholar
Hutton, K., Bailey, F. J. & Annison, E. F. (1970). British Journal of Nutrition 25, 165–173.CrossRefGoogle Scholar
Kaiser, F. E., Gehrke, C. W., Zumwalt, R. W. & Kuo, K. C. (1974). Journal of Chromatography 94, 113–133.CrossRefGoogle Scholar
Leng, R. A. (1982). British Journal of Nutrition 48, 399–416.CrossRefGoogle Scholar
Lindsay, D. B. & Armstrong, D. G. (1982). In Forage Protein in Ruminant Animal Production, Occasional Publication, British Society of Animal Production, no. 6, pp. 13–24 [Thomson, D. J., Beever, D. E. and Gunn, R. G. editors]. Thames Ditton: British Society of Animal Production.Google Scholar
McAllan, A. B. & Smith, R. H. (1972). Proceedings of the Nutrition Society 31, 24A.Google Scholar
McMillan, L. (1982). D-Amino acids in the ruminant digestive tract. PhD Thesis, University of Newcastle upon Tyne.Google Scholar
Mathers, J. C. & Miller, E. L. (1980). British Journal of Nutrition 43, 503–514.CrossRefGoogle Scholar
Mathison, G. W. & Milligan, L. P. (1971). British Journal of Nutrition 25, 351–366.CrossRefGoogle Scholar
Merry, R. J. & McAllan, A. B. (1983). British Journal of Nutrition 50, 701–709.CrossRefGoogle Scholar
Ministry of Agriculture, Fisheries and Food (1975). Energy Allowances and Feeding Systems for Ruminants: Technical Bulletin no. 33. London: H.M. Stationery Office.Google Scholar
Rooke, J. A., Greife, H. A. & Armstrong, D. G. (1984). Journal of Agricultural Science, Cambridge 102, 695–702.CrossRefGoogle Scholar
Rooke, J. A., Greife, H. A. & Armstrong, D. G. (1985). British Journal of Nutrition 53, 691–708.CrossRefGoogle Scholar
Schliefer, K. G. & Kandler, O. (1972). Bacteriological Reviews 36, 407–477.CrossRefGoogle Scholar
Siddons, R. C., Beever, D. E. & Nolan, J. V. (1982). British Journal of Nutrition 48, 377–390.CrossRefGoogle Scholar
Storm, E. & Ørskov, E. R. (1983). British Journal of Nutrition 50, 463–470.CrossRefGoogle Scholar
Vogels, G. D., Hoppe, W. F. & Stumm, C. K. (1980). Applied and Environmental Microbiology 40, 608–612.CrossRefGoogle Scholar
Williams, A. G. & Harfoot, C. G. (1976). Journal of General Microbiology 96, 125–136.CrossRefGoogle Scholar