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Inulin oligofructose attenuates metabolic syndrome in high-carbohydrate, high-fat diet-fed rats

  • Senthil A. Kumar (a1), Leigh C. Ward (a2) and Lindsay Brown (a1) (a3)
Abstract

Prebiotics alter bacterial content in the colon, and therefore could be useful for obesity management. We investigated the changes following addition of inulin oligofructose (IO) in the food of rats fed either a corn starch (C) diet or a high-carbohydrate, high-fat (H) diet as a model of diet-induced metabolic syndrome. IO did not affect food intake, but reduced body weight gain by 5·3 and 12·3 % in corn starch+inulin oligofructose (CIO) and high-carbohydrate, high-fat with inulin oligofructose (HIO) rats, respectively. IO reduced plasma concentrations of free fatty acids by 26·2 % and TAG by 75·8 % in HIO rats. IO increased faecal output by 93·2 %, faecal lipid excretion by 37·9 % and weight of caecum by 23·4 % and colon by 41·5 % in HIO rats. IO improved ileal morphology by reducing inflammation and improving the density of crypt cells in HIO rats. IO attenuated H diet-induced increases in abdominal fat pads (C 275 (sem 19), CIO 264 (sem 40), H 688 (sem 55), HIO 419 (sem 32) mg/mm tibial length), fasting blood glucose concentrations (C 4·5 (sem 0·1), CIO 4·2 (sem 0·1), H 5·2 (sem 0·1), HIO 4·3 (sem 0·1) mmol/l), systolic blood pressure (C 124 (sem 2), CIO 118 (sem 2), H 152 (sem 2), HIO 123 (sem 3) mmHg), left ventricular diastolic stiffness (C 22·9 (sem 0·6), CIO 22·9 (sem 0·5), H 27·8 (sem 0·5), HIO 22·6 (sem 1·2)) and plasma alanine transaminase (C 29·6 (sem 2·8), CIO 32·1 (sem 3·0), H 43·9 (sem 2·6), HIO 33·6 (sem 2·0) U/l). IO attenuated H-induced increases in inflammatory cell infiltration in the heart and liver, lipid droplets in the liver and plasma lipids as well as impaired glucose and insulin tolerance. These results suggest that increasing soluble fibre intake with IO improves signs of the metabolic syndrome by decreasing gastrointestinal carbohydrate and lipid uptake.

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Corresponding author
* Corresponding author: Professor L. Brown, email Lindsay.Brown@usq.edu.au
References
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1. Granado-Lorencio, F & Hernández-Alvarez, E (2016) Functional foods and health effects: a nutritional biochemistry perspective. Curr Med Chem (epublication ahead of print version 14 June 2016).
2. Brown, L, Poudyal, H & Panchal, SK (2015) Functional foods as potential therapeutic options for metabolic syndrome. Obes Rev 16, 914941.
3. Leach, JD & Sobolik, KD (2010) High dietary intake of prebiotic inulin-type fructans in the prehistoric Chihuahuan Desert. Br J Nutr 103, 15581561.
4. Florowska, A, Krygier, K, Florowski, T, et al. (2016) Prebiotics as functional food ingredients preventing diet-related diseases. Food Funct 18, 21472155.
5. Jones, JM (2014) CODEX-aligned dietary fiber definitions help to bridge the ‘fiber gap’. Nutr J 13, 34.
6. Bonnema, AL, Kolberg, LW, Thomas, W, et al. (2010) Gastrointestinal tolerance of chicory inulin products. J Am Diet Assoc 110, 865868.
7. Mensink, MA, Frijlink, HW, van der Voort Maarschalk, K, et al. (2015) Inulin, a flexible oligosaccharide II: review of its pharmaceutical applications. Carbohydr Polym 134, 418428.
8. Mensink, MA, Frijlink, HW, van der Voort Maarschalk, K, et al. (2015) Inulin, a flexible oligosaccharide I: review of its physicochemical characteristics. Carbohydr Polym 130, 405419.
9. Niness, KR (1999) Inulin and oligofructose: what are they? J Nutr 129, Suppl. 7, 1402S1406S.
10. Maurer, AD, Eller, LK, Hallam, MC, et al. (2010) Consumption of diets high in prebiotic fiber or protein during growth influences the response to a high fat and sucrose diet in adulthood in rats. Nutr Metab (Lond) 7, 77.
11. Cani, PD, Dewever, C & Delzenne, NM (2004) Inulin-type fructans modulate gastrointestinal peptides involved in appetite regulation (glucagon-like peptide-1 and ghrelin) in rats. Br J Nutr 92, 521526.
12. Cani, PD, Neyrinck, AM, Maton, N, et al. (2005) Oligofructose promotes satiety in rats fed a high-fat diet: involvement of glucagon-like peptide-1. Obes Res 13, 10001007.
13. Beylot, M (2005) Effects of inulin-type fructans on lipid metabolism in man and in animal models. Br J Nutr 93, Suppl. 1, S163S168.
14. Panchal, SK, Poudyal, H, Iyer, A, et al. (2011) High-carbohydrate, high-fat diet-induced metabolic syndrome and cardiovascular remodeling in rats. J Cardiovasc Pharmacol 57, 611624.
15. Coudray, C, Tressol, JC, Gueux, E, et al. (2003) Effects of inulin-type fructans of different chain length and type of branching on intestinal absorption and balance of calcium and magnesium in rats. Eur J Nutr 42, 9198.
16. Roberfroid, MB (2007) Inulin-type fructans: functional food ingredients. J Nutr 137, 2493S2502S.
17. Poudyal, H, Panchal, S & Brown, L (2010) Comparison of purple carrot juice and beta-carotene in a high-carbohydrate, high-fat diet-fed rat model of the metabolic syndrome. Br J Nutr 104, 13221332.
18. Poudyal, H, Panchal, SK, Ward, LC, et al. (2013) Effects of ALA, EPA and DHA in high-carbohydrate, high-fat diet-induced metabolic syndrome in rats. J Nutr Biochem 24, 10411052.
19. de Simone, G, di Lorenzo, L, Costantino, G, et al. (1988) Echocardiographic indexes of left ventricular contractility. Effect of load manipulation in arterial hypertension. Jpn Heart J 29, 151160.
20. Kumar, SA, Magnusson, M, Ward, LC, et al. (2015) Seaweed supplements normalise metabolic, cardiovascular and liver responses in high-carbohydrate, high-fat fed rats. Mar Drugs 13, 788805.
21. Delmée, E, Cani, PD, Gual, G, et al. (2006) Relation between colonic proglucagon expression and metabolic response to oligofructose in high fat diet-fed mice. Life Sci 79, 10071013.
22. Adam, CL, Williams, PA, Garden, KE, et al. (2015) Dose-dependent effects of a soluble dietary fibre (pectin) on food intake, adiposity, gut hypertrophy and gut satiety hormone secretion in rats. PLOS ONE 10, e0115438.
23. Folch, J, Lees, M & Sloane Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497509.
24. Poudyal, H, Panchal, SK, Waanders, J, et al. (2012) Lipid redistribution by alpha-linolenic acid-rich chia seed inhibits stearoyl-CoA desaturase-1 and induces cardiac and hepatic protection in diet-induced obese rats. J Nutr Biochem 23, 153162.
25. Sagher, FA, Dodge, JA, Johnston, CF, et al. (1991) Rat small intestinal morphology and tissue regulatory peptides: effects of high dietary fat. Br J Nutr 65, 2128.
26. Kerem, M, Salman, B, Pasaoglu, H, et al. (2008) Effects of microalgae Chlorella species crude extracts on intestinal adaptation in experimental short bowel syndrome. World J Gastroenterol 14, 45124517.
27. Roberfroid, M (1993) Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. Crit Rev Food Sci Nutr 33, 103148.
28. Jacobsen, R, Lorenzen, JK, Toubro, S, et al. (2005) Effect of short-term high dietary calcium intake on 24-h energy expenditure, fat oxidation, and fecal fat excretion. Int J Obes (Lond) 29, 292301.
29. Friedrich, M, Petzke, KJ, Raederstorff, D, et al. (2012) Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet. Int J Obes (Lond) 36, 735743.
30. Shin, AC, Zheng, H, Townsend, RL, et al. (2013) Longitudinal assessment of food intake, fecal energy loss, and energy expenditure after Roux-en-Y gastric bypass surgery in high-fat-fed obese rats. Obes Surg 23, 531540.
31. Westerterp, KR, Smeets, A, Lejeune, MP, et al. (2008) Dietary fat oxidation as a function of body fat. Am J Clin Nutr 87, 132135.
32. Delzenne, NM, Cani, PD & Neyrinck, AM (2007) Modulation of glucagon-like peptide 1 and energy metabolism by inulin and oligofructose: experimental data. J Nutr 137, 2547S2551S.
33. Parnell, JA & Reimer, RA (2012) Prebiotic fibres dose-dependently increase satiety hormones and alter Bacteroidetes and Firmicutes in lean and obese JCR:LA-cp rats. Br J Nutr 107, 601613.
34. Verhoef, SP, Meyer, D & Westerterp, KR (2011) Effects of oligofructose on appetite profile, glucagon-like peptide 1 and peptide YY3-36 concentrations and energy intake. Br J Nutr 106, 17571762.
35. Chaudhri, OB, Salem, V, Murphy, KG, et al. (2008) Gastrointestinal satiety signals. Annu Rev Physiol 70, 239255.
36. Slavin, J (2013) Fiber and prebiotics: mechanisms and health benefits. Nutrients 5, 14171435.
37. Kaur, N & Gupta, AK (2002) Applications of inulin and oligofructose in health and nutrition. J Biosci 27, 703714.
38. Akalin, AS & Erisir, D (2008) Effects of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream. J Food Sci 73, M184M188.
39. Little, TJ, Horowitz, M & Feinle-Bisset, C (2007) Modulation by high-fat diets of gastrointestinal function and hormones associated with the regulation of energy intake: implications for the pathophysiology of obesity. Am J Clin Nutr 86, 531541.
40. Park, JH, Kwon, OD, Ahn, SH, et al. (2013) Fatty diets retarded the propulsive function of and attenuated motility in the gastrointestinal tract of rats. Nutr Res 33, 228234.
41. Roberfroid, MB & Delzenne, NM (1998) Dietary fructans. Annu Rev Nutr 18, 117143.
42. Lobo, AR, Filho, JM, Alvares, EP, et al. (2009) Effects of dietary lipid composition and inulin-type fructans on mineral bioavailability in growing rats. Nutrition 25, 216225.
43. Madden, JA & Hunter, JO (2002) A review of the role of the gut microflora in irritable bowel syndrome and the effects of probiotics. Br J Nutr 88, Suppl. 1, S67S72.
44. Veereman, G (2007) Pediatric applications of inulin and oligofructose. J Nutr 137, 2585S2589S.
45. Zhang, C, Yin, A, Li, H, et al. (2015) Dietary modulation of gut microbiota contributes to alleviation of both genetic and simple obesity in children. EBioMedicine 2, 966982.
46. Brun, P, Castagliuolo, I, Di Leo, V, et al. (2007) Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 292, G518G525.
47. Cani, PD, Bibiloni, R, Knauf, C, et al. (2008) Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57, 14701481.
48. DiBaise, JK, Frank, DN & Mathur, R (2012) Impact of the gut microbiota on the development of obesity: current concepts. Am J Gastroenterol Suppl 1, 2227.
49. Ghanim, H, Abuaysheh, S, Sia, CL, et al. (2009) Increase in plasma endotoxin concentrations and the expression of Toll-like receptors and suppressor of cytokine signaling-3 in mononuclear cells after a high-fat, high-carbohydrate meal: implications for insulin resistance. Diabetes Care 32, 22812287.
50. Stenman, LK, Waget, A, Garret, C, et al. (2014) Potential probiotic Bifidobacterium animalis ssp. lactis 420 prevents weight gain and glucose intolerance in diet-induced obese mice. Benef Microbes 5, 437445.
51. Griffiths, EA, Duffy, LC, Schanbacher, FL, et al. (2004) In vivo effects of bifidobacteria and lactoferrin on gut endotoxin concentration and mucosal immunity in Balb/c mice. Dig Dis Sci 49, 579589.
52. Rodes, L, Saha, S, Tomaro-Duchesneau, C, et al. (2014) Microencapsulated Bifidobacterium longum subsp. infantis ATCC 15697 favorably modulates gut microbiota and reduces circulating endotoxins in F344 rats. Biomed Res Int 2014, 602832.
53. Amar, J, Chabo, C, Waget, A, et al. (2011) Intestinal mucosal adherence and translocation of commensal bacteria at the early onset of type 2 diabetes: molecular mechanisms and probiotic treatment. EMBO Mol Med 3, 559572.
54. Janssen, P, Rotondo, A, Mule, F, et al. (2013) Review article: a comparison of glucagon-like peptides 1 and 2. Aliment Pharmacol Ther 37, 1836.
55. Cani, PD, Possemiers, S, Van de Wiele, T, et al. (2009) Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 58, 10911103.
56. Nilsson, A, Ostman, E, Preston, T, et al. (2008) Effects of GI vs content of cereal fibre of the evening meal on glucose tolerance at a subsequent standardized breakfast. Eur J Clin Nutr 62, 712720.
57. Luo, J, Rizkalla, SW, Alamowitch, C, et al. (1996) Chronic consumption of short-chain fructooligosaccharides by healthy subjects decreased basal hepatic glucose production but had no effect on insulin-stimulated glucose metabolism. Am J Clin Nutr 63, 939945.
58. Lee, KU, Park, JY, Kim, CH, et al. (1996) Effect of decreasing plasma free fatty acids by acipimox on hepatic glucose metabolism in normal rats. Metabolism 45, 14081414.
59. Stanhope, KL & Havel, PJ (2008) Fructose consumption: potential mechanisms for its effects to increase visceral adiposity and induce dyslipidemia and insulin resistance. Curr Opin Lipidol 19, 1624.
60. Weisberg, SP, McCann, D, Desai, M, et al. (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112, 17961808.
61. Damaso, AR, do Prado, WL, de Piano, A, et al. (2008) Relationship between nonalcoholic fatty liver disease prevalence and visceral fat in obese adolescents. Dig Liver Dis 40, 132139.
62. Fabbrini, E, Sullivan, S & Klein, S (2010) Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and clinical implications. Hepatology 51, 679689.
63. Hietbrink, F, Besselink, MG, Renooij, W, et al. (2009) Systemic inflammation increases intestinal permeability during experimental human endotoxemia. Shock 32, 374378.
64. Lau, WL, Kalantar-Zadeh, K & Vaziri, ND (2015) The gut as a source of inflammation in chronic kidney disease. Nephron 130, 9298.
65. Huang, EY, Leone, VA, Devkota, S, et al. (2013) Composition of dietary fat source shapes gut microbiota architecture and alters host inflammatory mediators in mouse adipose tissue. JPEN J Parenter Enteral Nutr 37, 746754.
66. Gregor, MF & Hotamisligil, GS (2011) Inflammatory mechanisms in obesity. Annu Rev Immunol 29, 415445.
67. Barbaro, NR, Fontana, V, Modolo, R, et al. (2015) Increased arterial stiffness in resistant hypertension is associated with inflammatory biomarkers. Blood Press 24, 713.
68. Pietri, P, Vyssoulis, G, Vlachopoulos, C, et al. (2006) Relationship between low-grade inflammation and arterial stiffness in patients with essential hypertension. J Hypertens 24, 22312238.
69. Juskewitch, JE, Knudsen, BE, Platt, JL, et al. (2012) LPS-induced murine systemic inflammation is driven by parenchymal cell activation and exclusively predicted by early MCP-1 plasma levels. Am J Pathol 180, 3240.
70. Kararigas, G, Dworatzek, E, Petrov, G, et al. (2014) Sex-dependent regulation of fibrosis and inflammation in human left ventricular remodelling under pressure overload. Eur J Heart Fail 16, 11601167.
71. Nicoletti, A & Michel, JB (1999) Cardiac fibrosis and inflammation: interaction with hemodynamic and hormonal factors. Cardiovasc Res 41, 532543.
72. Dietitians Association of Australia (1983) For the public: Smart Eating for you: Nutrition Information A-Z: Fibre. http://daa.asn.au/?page_id=800.
73. Reagan-Shaw, S, Nihal, M & Ahmad, N (2008) Dose translation from animal to human studies revisited. FASEB J 22, 659661.
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