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Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men

  • Yifan Yang (a1), Leigh Breen (a1), Nicholas A. Burd (a1), Amy J. Hector (a1), Tyler A. Churchward-Venne (a1), Andrea R. Josse (a1), M. A. Tarnopolsky (a2) and Stuart M. Phillips (a1)...
Abstract

Feeding stimulates robust increases in muscle protein synthesis (MPS); however, ageing may alter the anabolic response to protein ingestion and the subsequent aminoacidaemia. With this as background, we aimed to determine in the present study the dose–response of MPS with the ingestion of isolated whey protein, with and without prior resistance exercise, in the elderly. For the purpose of this study, thirty-seven elderly men (age 71 (sd 4) years) completed a bout of unilateral leg-based resistance exercise before ingesting 0, 10, 20 or 40 g of whey protein isolate (W0–W40, respectively). Infusion of l-[1-13C]leucine and l-[ring-13C6]phenylalanine with bilateral vastus lateralis muscle biopsies were used to ascertain whole-body leucine oxidation and 4 h post-protein consumption of MPS in the fed-state of non-exercised and exercised leg muscles. It was determined that whole-body leucine oxidation increased in a stepwise, dose-dependent manner. MPS increased above basal, fasting values by approximately 65 and 90 % for W20 and W40, respectively (P < 0·05), but not with lower doses of whey. While resistance exercise was generally effective at stimulating MPS, W20 and W40 ingestion post-exercise increased MPS above W0 and W10 exercised values (P < 0·05) and W40 was greater than W20 (P < 0·05). Based on the study, the following conclusions were drawn. At rest, the optimal whey protein dose for non-frail older adults to consume, to increase myofibrillar MPS above fasting rates, was 20 g. Resistance exercise increases MPS in the elderly at all protein doses, but to a greater extent with 40 g of whey ingestion. These data suggest that, in contrast to younger adults, in whom post-exercise rates of MPS are saturated with 20 g of protein, exercised muscles of older adults respond to higher protein doses.

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Corresponding author
*Corresponding author: S. M. Phillips, fax +1 905 523 6011, email phillis@mcmaster.ca
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17V Kumar , A Selby , D Rankin , et al. (2009) Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men. J Physiol 587, 211217.

6E Volpi , B Mittendorfer , BB Rasmussen , et al. (2000) The response of muscle protein anabolism to combined hyperaminoacidemia and glucose-induced hyperinsulinemia is impaired in the elderly. J Clin Endocrinol Metab 85, 44814490.

7CS Katsanos , H Kobayashi , M Sheffield-Moore , et al. (2006) A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am J Physiol Endocrinol Metab 291, E381E387.

8D Paddon-Jones , M Sheffield-Moore , XJ Zhang , et al. (2004) Amino acid ingestion improves muscle protein synthesis in the young and elderly. Am J Physiol Endocrinol Metab 286, E321E328.

9B Pennings , R Koopman , M Beelen , et al. (2011) Exercising before protein intake allows for greater use of dietary protein-derived amino acids for de novo muscle protein synthesis in both young and elderly men. Am J Clin Nutr 93, 322331.

10MJ Drummond , HC Dreyer , B Pennings , et al. (2008) Skeletal muscle protein anabolic response to resistance exercise and essential amino acids is delayed with aging. J Appl Physiol 104, 14521461.

11V Kumar , P Atherton , K Smith , et al. (2009) Human muscle protein synthesis and breakdown during and after exercise. J Appl Physiol 106, 20262039.

12JC Anthony , AK Reiter , TG Anthony , et al. (2002) Orally administered leucine enhances protein synthesis in skeletal muscle of diabetic rats in the absence of increases in 4E-BP1 or S6K1 phosphorylation. Diabetes 51, 928936.

13P Atherton , K Smith , T Etheridge , et al. (2010) Distinct anabolic signalling responses to amino acids in C2C12 skeletal muscle cells. Amino Acids 38, 15331539.

14NA Burd , JE Tang , DR Moore , et al. (2009) Exercise training and protein metabolism: influences of contraction, protein intake, and sex-based differences. J Appl Physiol 106, 16921701.

15I Rieu , M Balage , C Sornet , et al. (2006) Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia. J Physiol 575, 305315.

19HC Dreyer , MJ Drummond , B Pennings , et al. (2008) Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am J Physiol Endocrinol Metab 294, E392E400.

20OC Witard , M Tieland , M Beelen , et al. (2009) Resistance exercise increases postprandial muscle protein synthesis in humans. Med Sci Sports Exerc 41, 144154.

22DR Moore , MJ Robinson , JL Fry , et al. (2009) Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr 89, 161168.

23JE Tang , JJ Manolakos , GW Kujbida , et al. (2007) Minimal whey protein with carbohydrate stimulates muscle protein synthesis following resistance exercise in trained young men. Appl Physiol Nutr Metab 32, 11321138.

24M Sheffield-Moore , CW Yeckel , E Volpi , et al. (2004) Postexercise protein metabolism in older and younger men following moderate-intensity aerobic exercise. Am J Physiol Endocrinol Metab 287, E513E522.

26JE Tang , DR Moore , GW Kujbida , et al. (2009) Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol 107, 987992.

27B Pennings , Y Boirie , JMG Senden , et al. (2011) Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr 93, 9971005.

30S Fisher , KJ Ottenbacher , JS Goodwin , et al. (2009) Short physical performance battery in hospitalized older adults. Aging Clin Exp Res 21, 445452.

31NA Burd , AM Holwerda , KC Selby , et al. (2010) Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol 588, 31193130.

32NA Burd , DW West , T Rerecich , et al. (2011) Validation of a single biopsy approach and bolus protein feeding to determine myofibrillar protein synthesis in stable isotope tracer studies. Nutr Metab 8, 15.

33EI Glover , SM Phillips , BR Oates , et al. (2008) Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion. J Physiol 586, 60496061.

34DR Moore , SM Phillips , JA Babraj , et al. (2005) Myofibrillar and collagen protein synthesis in human skeletal muscle in young men after maximal shortening and lengthening contractions. Am J Physiol Endocrinol Metab 288, E1153E1159.

35DR Moore , JE Tang , NA Burd , et al. (2009) Differential stimulation of myofibrillar and sarcoplasmic protein synthesis with protein ingestion at rest and after resistance exercise. J Physiol 587, 897904.

36GI Smith , DT Villareal , CP Lambert , et al. (2010) Timing of the initial muscle biopsy does not affect the measured muscle protein fractional synthesis rate during basal, postabsorptive conditions. J Appl Physiol 108, 363368.

37MA Tarnopolsky , SA Atkinson , JD MacDougall , et al. (1991) Whole body leucine metabolism during and after resistance exercise in fed humans. Med Sci Sports Exerc 23, 326333.

39D Paddon-Jones & BB Rasmussen (2009) Dietary protein recommendations and the prevention of sarcopenia. Curr Opin Clin Nutr Metab Care 12, 8690.

42D Paddon-Jones , M Sheffield-Moore , CS Katsanos , et al. (2006) Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein. Exp Gerontol 41, 215219.

44M Leenders , LB Verdijk , L van der Hoeven , et al. (2011) Prolonged leucine supplementation does not augment muscle mass or affect glycemic control in elderly type 2 diabetic men. J Nutr 141, 10701076.

45S Verhoeven , K Vanschoonbeek , LB Verdijk , et al. (2009) Long-term leucine supplementation does not increase muscle mass or strength in healthy elderly men. Am J Clin Nutr 89, 14681475.

46E Borsheim , QU Bui , S Tissier , et al. (2008) Effect of amino acid supplementation on muscle mass, strength and physical function in elderly. Clin Nutr 27, 189195.

47R Scognamiglio , A Avogaro , C Negut , et al. (2004) The effects of oral amino acid intake on ambulatory capacity in elderly subjects. Aging Clin Exp Res 16, 443447.

49TB Symons , M Sheffield-Moore , MM Mamerow , et al. (2011) The anabolic response to resistance exercise and a protein rich meal is not diminished by age. J Nutr Health Aging 15, 376381.

50GL Onambele-Pearson , L Breen & CE Stewart (2010) Influences of carbohydrate plus amino acid supplementation on differing exercise intensity adaptations in older persons: skeletal muscle and endocrine responses. Age 32, 125138.

51KJ Dideriksen , S Reitelseder , SG Petersen , et al. (2011) Stimulation of muscle protein synthesis by whey and caseinate ingestion after resistance exercise in elderly individuals. Scand J Med Sci Sports 21, e372e383.

52R Koopman , M Beelen , T Stellingwerff , et al. (2007) Coingestion of carbohydrate with protein does not further augment postexercise muscle protein synthesis. Am J Physiol Endocrinol Metab 293, E833E842.

53AW Staples , NA Burd , DW West , et al. (2011) Carbohydrate does not augment exercise-induced protein accretion versus protein alone. Med Sci Sports Exerc 43, 11541161.

54EL Glynn , CS Fry , MJ Drummond , et al. (2010) Muscle protein breakdown has a minor role in the protein anabolic response to essential amino acid and carbohydrate intake following resistance exercise. Am J Physiol Regul Integr Comp Physiol 299, R533R540.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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