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A double stable isotope technique for measuring iron absorption in infants

Published online by Cambridge University Press:  17 March 2008

Peter Kastenmayer ,
Lena Davidsson ,
Pilar Galanz ,
Françise Cherouvrier ,
Serge Hercberg  and
Richard F. Hurrell
Peter Kastenmayer
Affiliation:
Nestlé Research Centre, PO Box 44, CH-I000 Lausanne 26, Switzerland
Lena Davidsson
Affiliation:
Nestlé Research Centre, PO Box 44, CH-I000 Lausanne 26, Switzerland
Pilar Galanz
Affiliation:
Centre de Recherche sur les Anémies Nutritionnelles, CNAM, 2 rue Conté, F-75003 Paris, France
Françise Cherouvrier
Affiliation:
Centre de Recherche sur les Anémies Nutritionnelles, CNAM, 2 rue Conté, F-75003 Paris, France
Serge Hercberg
Affiliation:
Centre de Recherche sur les Anémies Nutritionnelles, CNAM, 2 rue Conté, F-75003 Paris, France
Richard F. Hurrell
Affiliation:
Nestlé Research Centre, PO Box 44, CH-I000 Lausanne 26, Switzerland
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Abstract

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A stable isotope technique has been developed which uses 57Fe and 58Fe as labels and which enables the simultaneous measurement of Fe absorption from two test meals in infants. The method was evaluated by measuring Fe absorption from a commercial whey-adjusted infant formula in nine healthy infants aged 13–25 weeks. Each infant was fed 210 ml formula, labelled with either 57Fe or 58Fe, on four consecutive mornings, in random order. The total Fe content in each feed was 2.5 mg Fe; either as 2.5 mg 57Fe, or 0 6 mg 58Fe plus 1.9 mg Fe with normal isotopic composition. Isotopic enrichment of Fe in erythrocytes was measured by thermal ionization mass spectrometry 14 d after the last administration, and Fe absorption was calculated based on isotope ratio shifts, total circulating Fe and intake of each isotope. Geometric mean absorption for the 57Fe and 58Fe labels was 6.72 and 658% respectively, and the absorption of the two isotopes was not significantly different (Student's paired t test). By this technique, paired comparisons of Fe absorption can be obtained and systematic studies of the influence of dietary factors on Fe absorption during infancy can he conducted.

Keywords

IronStable isotopesInfantsThermal ionization mass spectrometry
Type
Research Article
Information
British Journal of Nutrition , Volume 71 , Issue 3 , March 1994 , pp. 411 - 424
Copyright
Copyright © The Nutrition Society 1994

References

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