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Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]α-linolenic acid to longer-chain fatty acids and partitioning towards β-oxidation in older men

  • Graham C. Burdge (a1), Yvonne E. Finnegan (a2), Anne M. Minihane (a2), Christine M. Williams (a2) and Stephen A. Wootton (a1)...
Abstract

The effect of increased dietary intakes of α-linolenic acid (ALNA) or eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) for 2 months upon plasma lipid composition and capacity for conversion of ALNA to longer-chain metabolites was investigated in healthy men (52 (SD 12) years). After a 4-week baseline period when the subjects substituted a control spread, a test meal containing [U-13C]ALNA (700 mg) was consumed to measure conversion to EPA, docosapentaenoic acid (DPA) and DHA over 48 h. Subjects were then randomised to one of three groups for 8 weeks before repeating the tracer study: (1) continued on same intake (control, n 5); (2) increased ALNA intake (10 g/d, n 4); (3) increased EPA+DHA intake (1·5 g/d, n 5). At baseline, apparent fractional conversion of labelled ALNA was: EPA 2·80, DPA 1·20 and DHA 0·04 %. After 8 weeks on the control diet, plasma lipid composition and [13C]ALNA conversion remained unchanged compared with baseline. The high-ALNA diet resulted in raised plasma triacylglycerol-EPA and -DPA concentrations and phosphatidylcholine-EPA concentration, whilst [13C]ALNA conversion was similar to baseline. The high-(EPA+DHA) diet raised plasma phosphatidylcholine-EPA and -DHA concentrations, decreased [13C]ALNA conversion to EPA (2-fold) and DPA (4-fold), whilst [13C]ALNA conversion to DHA was unchanged. The dietary interventions did not alter partitioning of ALNA towards β-oxidation. The present results indicate ALNA conversion was down-regulated by increased product (EPA+DHA) availability, but was not up-regulated by increased substrate (ALNA) consumption. This suggests regulation of ALNA conversion may limit the influence of variations in dietary n-3 fatty acid intake on plasma lipid compositions.

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      Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]α-linolenic acid to longer-chain fatty acids and partitioning towards β-oxidation in older men
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      Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]α-linolenic acid to longer-chain fatty acids and partitioning towards β-oxidation in older men
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      Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]α-linolenic acid to longer-chain fatty acids and partitioning towards β-oxidation in older men
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Corresponding author
*Corresponding author: Dr Graham C. Burdge, fax +44 23 804945, email g.c.burdge@soton.ac.uk
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T Babcock , WS Helton & NJ Espat (2000) Eicosapentaenoic acid (EPA): an antiinflammatory omega-3 fat with potential clinical applications. Nutrition 16, 11161118.

JK Chan , BE McDonald , JM Gerrard , VM Bruce , BJ Weaver & BJ Holub (1993) Effect of dietary α-linolenic acid and its ratio to linoleic acid on platelet and plasma fatty acids and thrombogenesis. Lipids 28, 811817.

EA Emken , RO Adolf , SM Duval & GJ Nelson (1999) Effect of dietary docosahexaenoic acid on desaturation and uptake in vivo of isotope-labeled oleic, linoleic and linolenic acids by male subjects. Lipids 34, 785798.

EA Emken , RO Adolf , S Duval , G Nelson & P Benito (2002) Effect of dietary conjugated linoleic acid (CLA) on metabolism of isotope-labeled oleic, linoleic and CLA isomers in women. Lipids 37, 741750.

EA Emken , RO Adolf & RM Gully (1994) Dietary linoleic acid influences desaturation and acylation of deuterium-labeled linoleic and linolenic acids in young adult males. Biochim Biophys Acta 1213, 277288.

O Ezaki , M Takahashi & T Shigematsu (1999) Long-term effects of dietary alpha linoleic acid from perilla oil on serum fatty acid composition and on the risk factors of coronary heart disease in Japanese elderly subjects. ((Tokyo)) J Nutr Sci Vitamin 45, 759772.

DL Hartwell & CJ Henry (2001) Comparison of a self administered quantitative food amount frequency questionnaire with 4-day estimated food records. Int J Food Sci Nutr 52, 151159.

AE Jones , JL Murphy , M Stolinski & SA Wootton (1998) The effect of age and gender on the metabolic disposal of 1-13C palmitic acid. Eur J Clin Nutr 52, 2228.

AE Jones , M Stolinski , RD Smith , JL Murphy & SA Wootton (1999) Effect of fatty acid chain length and saturation on the gastrointestinal handling and metabolic disposal of dietary fatty acids in women. Br J Nutr 81, 3743.

PL McLennan (2001) Myocardial membrane fatty acids and the antiarrhythmic actions of dietary fish oil in animal models. Lipids 36, Suppl., S111S114.

N Salem , R Pawlosky , B Wegher & J Hibbeln (1999) In vivo conversion of linoleic acid to arachidonic acid in human adults. Prostaglandins Leukot Essent Fatty Acids 60, 407410.

TAB Sanders & F Rosahani (1983) The influence of different types of n-3 polyunsaturated fatty acids on blood lipids and platelet function in healthy volunteers. Clin Sci 64, 9199.

H Shimokawa (2001) Beneficial effects of eicosapentaenoic acid on endothelial vasodilator functions in animals and humans. World Rev Nutr Diet 88, 100108.

AP Simopoulos (1997) Omega-3 fatty acids in the prevention-management of cardiovascular disease. Can J Pharmacol 75, 234239.

AP Simopoulos , A Leaf & N Salem (1999) Essentiality of and recommended dietary intakes for n-6 and n-3 fatty acids. Ann Nutr Metab 43, 127130.

H Sprecher (2000) Metabolism of highly unsaturated n-3 and n-6 fatty acids. Biochim Biophys Acta 1486, 219231.

SHF Vermunt , RP Mensink , AMG Simonis & G Hornstra (1999) Effects of age and dietary n-3 fatty acids on the metabolism of [13C]-α-linolenic acid. (Abstr). Lipids 34, S127 Abstr.

SHF Vermunt , RP Mensink , AMG Simonis & G Hornstra (2000) Effects of dietary α-linolenic acid on the conversion and oxidation of [13C]-α-linolenic acid. Lipids 35, 137142.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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