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Effect of dietary quercetin and sphingomyelin on intestinal nutrient absorption and animal growth

  • J. Barrenetxe (a1), P. Aranguren (a1), A. Grijalba (a2), J.M. Martínez-Peñuela (a3), F. Marzo (a1) and E. Urdaneta (a1)...

Abstract

Research on cancer and other conditions has shown flavonoids and sphingolipids to be food components capable of exerting chemoprotective action. Nevertheless, little is known about their effects on healthy individuals and their potential usefulness as therapeutic agents. The present study examined the possible action of a dietary flavonoid, quercetin, and a sphingolipid, sphingomyelin, as functional foods in healthy animals. In particular, the effect on animal growth of supplementing a conventional diet with one or other of these substances (0·5% quercetin and 0·05% sphingomyelin) was considered. Possible action affecting intestinal physiology was also analysed by measuring the uptake of sugar and dipeptide, mediated by the Na+-dependent sugar transporter SGLT1 and the dipeptide Na+/H+ exchanger PEPT1 respectively, and the activity of related intestinal enzymes such as sucrase, maltase and aminopeptidase N. Both substances seemed to modify small intestinal activity in healthy mice, altering intestinal enzymatic activity and nutrient uptake. These effects observed in the small intestine did not impair normal development of the animals, as no differences in serum biochemical parameters or in organ and body weights were found. The findings should help in elucidating the mechanisms of action of these food components with a view to their possible use in the prevention of certain pathological conditions.

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Corresponding author

*Corresponding author: fax +34 948 168930, email elena.urdaneta@unavarra.es

References

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Ader, PBlöck, M, Pietzsch, S & Wolffram, S 2001 Interaction of quercetin glucosides with the sodium/glucose cotransporter (SGLT1). Cancer Lett 162 175180.
Adibi, SA 2003 Regulation of the intestinal oligopeptide transporter (Pept-1) in health and disease. Am J Physiol Gastrointest Liver Physiol 285 G779G788.
Andria, GCucchiara, S, De Vizia, B, De Ritis, G, Masaka, G & Aurichio, S 1980 Brush border and cytosol peptidase activities of human small intestine in normal subjects and celiac patients. Pediatr Res 14 812818i.
Boyer, J, Brown, D & Liu, RH 2004 Uptake of quercetin and quercetin 3-glucoside from whole onion and apple peel extracts by Caco-2 cell monolayers. J Agric Food Chem 52 71727179.
Cermak, R, Landgraf, S & Wolffram, S 2004 Quercetin glucosides inhibit glucose uptake into brush-border-membrane vesicles of porcine jejunum. Br J Nutr 91 849855.
Dahlqvist, A 1964 Method for assay of intestinal disaccharidases. Anal Biochem 7 1825.
De Vries, JHM, Janssen, PL, Hollman, PCH, van Staveren, WA & Katan, MBCancer Lett 114. Consumption of quercetin and kaempferol in free-living subjects eating a variety of diets, 1997 114141144.
Dillehay, DL, Webb, SK, Schmelz, EM & Merrill, AH Jr J Nutr. Dietary sphingomyelin inhibits 1, 2-dimethylhydrazine-induced colon cancer in CF1 mice, 1994 615620.
Gee, JM, Du, Pont MS, Day, AJ, Plumb, GW, Williamson, G & Johnson, ITJ Nutr. Intestinal transport of quercetin glycosides in rats involves both deglycosylation and interaction with the hexose transport pathway, 2000 130 27652771.
Halliwell, B, Rafter, J & Jenner, J 2005 Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not?. Am J Clin Nutr 81 268276.
Hopfer, U, Sigrist-Nelson, K, Perotto, J & Murer, H 1975 Intestinal sugar transport: studies with isolated plasma membranes. Ann N Y Acad Sci 264 414427.
Katsura, T & Inui, K 2003 Intestinal absorption of drugs mediated by drug transporters: mechanisms and regulation. Drug Metab Pharmacokinet 18 115.
Lemonnier, LA, Dillehay, DL, Vespremi, MJ, Abrams, J, Brody, E & Schmelz, EM 2003 Sphingomyelin in the suppression of colon tumors: prevention versus intervention. Arch Biochem Biophys 419 129138.
Liu, RH 2004 Potential synergy of phytochemicals in cancer prevention:mechanism of action. J Nutr 134 34793485.
Merrill, AH Jr, Schmelz, EM, Wang, E, Dillehay, DL, Rice, LG, Meredith, F & Riley, RT 1997 Importance of sphingolipids and inhibitors of sphingolipid metabolism as components of animal diets. J Nutr 127 830S833S.
Moskaug, JO, Carlsen, H, Myhrstad, M & Blomhoff, R 2004 Molecular imaging of the biological effects of quercetin and quercetin rich foods. Mech Ageing Dev 125 315324.
Murota, K & Terao, J 2003 Antioxidative flavonoid quercetin: implication of its intestinal absorption and metabolism. Arch Biochem Biophys 417 1217.
Nemeth, K, Plumb, GW, Berrin, JG, Juge, N, Jacob, R, Naim, HY, Williamson, G, Swallow, DM & Kroon, PA 2003 Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flaronoid glycosides in humans. Eur J Nutr 42 2942.
O'Prey, J, Brown, J, Fleming, J & Harrison, PR 2003 Effects of dietary flavonoids on major signal transduction pathways in human epithelial cells. Biochem Pharmacol 66 20752088.
Ramachandra, R, Shetty, AK & Salimath, PV 2005 Quercetin alleviates activities of intestinal and renal disaccharidases in streptozotocininduced diabetic rats. Mol Nutr Food Res 49 355360.
Sang, KN & Sung, IK 2003 Egg sphingomyelin lowers the lymphatic absorption of cholesterol and a-tocopherol in rats. J Nutr 133 35713576.
Schmelz, EM, Dillehay, DL, Webb, SK, Reiter, A, Adams, J & Merrill, AH Jr 1996 Sphingomyelin consumption suppresses aberrant colonic crypt foci and increases the proportion of adenomas versus adenocarcinomas in CF1 mice treated with 1, 2-dimethylhydrazine: implications for dietary sphingolipids and colon carcinogenesis. Cancer Res 56 49364941.
Schmelz, EM, Sullards, MC, Dillehay, DL & Merrill, AH 2000 Colonic cell proliferation and aberrant crypt foci formation are inhibited by dairy glycosphingolipids in 1, 2-dimethylhydrazine-treated CF1 mice. J Nutr 130 522527.
Schmelz, EM, Roberts, PC, Kustin, EM, Jr Lemonnier, LA, Sullards, MC, Dillehay, DL & Merrill, AH Jr (2001) Modulation of intracellular bcatenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids Cancer Res. 61 67236729.
Shiraga, T, Miyamoto, K, Tanaka, H, Yamamoto, H, Taketani, Y, Morita, K, Tamai, I, Tsuji, A & Takeda, E 1999 Cellular and molecular mechanisms of dietary regulation on rat intestinal H+/peptide transporter PepT1. Gastroenterology 116 354362.
Shirazi-Beechey, SP, Davies, AG, Tebbutt, K, Dyer, J, Ellis, A, Taylor, CJ, Fairclough, P & Beechey, RB 1990 Preparation and properties of brush border membrane vesicles from human small intestine. Gastroenterology 98 676685.
Strobel, P, Allard, C, Pérez-Acle, T, Calderon, R, Aldunate, R & Leighton, F 2005 Myricetin, quercetin and catechin-gallate inhibit glucose uptake in isolated adipocytes. Biochem J 386 471478.
Uezato, T & Fujita, M 1983 Developmental transition of alkaline phosphatase from suckling to adult type in rat small intestine: molecular species and effect of injected cortisone and thyroxine. J Biochem 94 14831488.
Vesper, H, Schmelz, EM, Nikolova-Karakashian, MN, Dillehay, DL, Lynch, DV & Merrill, AH Jr 1999 Sphingolipids in food and the emerging importance of sphingolipids to nutrition. J Nutr 129 12391250.
Wenzel, U, Kuntz, S & Daniel, H 2001 Flavonoids with epidermal growth factor-receptor tyrosine kinase inhibitory activity stimulates PEPT1-mediated cefixime uptake into human epithelial cells. J Pharmacol Exp Ther 299 351357.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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