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The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick

Published online by Cambridge University Press:  09 March 2007

Assaf Geyra
Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76-100, Israel
Zehava Uni
Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76-100, Israel
David Sklan*
Faculty of Agriculture, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76-100, Israel
*Corresponding author: Dr D. Sklan, fax +1972 8 9489865, email
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The small intestines of hatching chicks undergo rapid developmental changes in the immediate post-hatch period when the birds are making the transition from endogenous nutrient supply from yolk to dependence on exogenous feed. This transition usually only begins 48 h or more after hatching, owing to logistical considerations of production. The effects of fasting for 48 h at different times during this critical period on small intestinal development and enterocyte dynamics were examined by morphometric determinations and use of staining for proliferative-cell nuclear antigen and 5-bromo-2-deoxyuridine. The effects of fasting were specific to both time of fasting and the intestinal segment examined. Decreased development was found in the duodenum and jejunum, but was less apparent in the ileum. Fasting between 0 and 48 h decreased crypt size in the duodenum and jejunum, the number of crypts per villus, crypt proliferation, villus area and the rate of enterocyte migration. Fasting at later times resulted in smaller effects, although the jejunum appeared to be the most sensitive of the intestinal segments. Growth was correlated with the number of cells in the crypts, the number of cells along the villus and the segment surface area. The common practice whereby feed is first available to chicks more than 48 h post-hatch may depress subsequent development.

Research Article
Copyright © The Nutrition Society 2001


Butzner, JD & Gall, S (1990) Impact of refeeding on intestinal development and function in infant rabbits subjected to protein energy malnutrition. Pediatric Research 27, 245251.Google Scholar
Feil, W, Lacy, ER, Wong, YM, Burger, D, Wenzl, E, Starlinger, M & Schiessel, R (1989) Rapid epithelial restitution of human and rabbit colonic mucosa. Gastroenterology 97, 685701.Google Scholar
Heitlingher, LA, Rossi, TM, Lee, P & Lebenthal, E (1991) Human intestinal disaccharidase activities: correlations with age, biopsy technique, and degree of villus atrophy. Journal of Pediatric Gastroenterology and Nutrition 12, 204208.Google Scholar
Hermos, JA, Mathan, M & Trier, JS (1971) DNA synthesis and proliferation by villus epithelial cells in fetal rats. Journal of Cell Biology 50, 255258.Google Scholar
Hodin, RA, Graham, JR, Meng, S & Upton, MP (1994) Temporal pattern of rat small intestinal gene expression with refeeding. American Journal of Physiology 266, G83G89.Google Scholar
Holt, PR, Wu, S & Yeh, KY (1986) Ileal hyperplastic response to starvation in the rat. American Journal of Physiology 251, G124G131.Google Scholar
Moran, ET & Reinhart, BS (1980) Poult yolk sac amount and composition upon placement: effect of breeder age, egg weight, sex and subsequent change with feeding or fasting. Poultry Science 59, 15211528.Google Scholar
National Research Council (1994) Nutrient Requirements of Poulry 9th ed. Washington, DC: National Academy of Science.Google Scholar
Noy, Y & Sklan, D (1998a) Metabolic responses to early nutrition. Journal of Applied Poultry Research 7, 437451.Google Scholar
Noy, Y & Sklan, D (1999) Energy utilization in newly hatched chicks. Poultry Science 78, 17501756.Google Scholar
Noy, Y, Uni, Z & Sklan, D (1996) Routes of yolk utilisation in the newly hatched chick. British Poultry Science 37, 987996.Google Scholar
Nunez, MC, Bueno, JD, Ayudarte, MV, Almendros, A, Rios, A, Suaarez, MD & Gil, A (1996) Dietary restriction induces biochemical and morphometric changes in the small intestine of nursing piglets. Journal of Nutrition 126, 933944.Google Scholar
Ortega, MA, Gil, A & Sanchez-Ponzo, A (1995) Maturation status of small intestine epithelium in rats deprived of dietary nucleotides. Life Sciences 19, 16231630.Google Scholar
Reisenfeld, G, Geva, A & Hurwitz, S (1982) Glucose homeostasis in the chicken. Journal of Nutrition 112, 22612266.Google Scholar
Shamoto, K & Yamauchi, K (2000) Recovery responses of chick intestinal villus morphology to different refeeding procedures. Poultry Science 79, 718723.Google Scholar
Simon, TC & Gordon, JI (1995) Intestinal epithelial cell differentiation: new insights from mice, flies and nematodes. Current Opinion in Genetics and Development 5, 577586.Google Scholar
Sklan, D, Cohen, N & Hurwitz, S (1996) Intestinal uptake and metabolism of fatty acids in the chick. Poultry Science 75, 11041108.Google Scholar
Uni, Z, Ganot, S & Sklan, D (1998b) Posthatch development of mucosal function in the broiler small intestine. Poultry Science 77, 7582.Google Scholar
Uni, Z, Geyra, A, Ben-Hur, & Sklan, D (2000) Small intestinal development in the young chick: crypt formation and enterocyte proliferation and migration. British Poultry Science 39, 544551.Google Scholar
Uni, Z, Platin, R & Sklan, D (1998) Cell proliferation in chicken intestinal epithelium occurs both in the crypt and along the villus. Journal of Comparative Physiology 168, 241247.Google Scholar
Yamauchi, K, Kamisoyama, H & Isshiki, Y (1996) Effects of fasting and refeeding on structures of the intestinal villi and epithelial cells in White Leghorn hens. British Poultry Science 37, 909921.Google Scholar