Skip to main content
    • Aa
    • Aa
  • Access
  • Cited by 55
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Deng, Yinghui Pang, Yanhua Guo, Yafei Ren, Yufeng Wang, Fen Liao, Xiali and Yang, Bo 2016. Host-guest inclusion systems of daidzein with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and sulfobutyl ether-β-cyclodextrin (SBE-β-CD): Preparation, binding behaviors and water solubility. Journal of Molecular Structure, Vol. 1118, p. 307.

    Kolodziejczyk-Czepas, Joanna Sieradzka, Malgorzata Wachowicz, Barbara Nowak, Pawel Oleszek, Wieslaw and Stochmal, Anna 2016. The anti-adhesive and anti-aggregatory effects of phenolics from Trifolium species in vitro. Molecular and Cellular Biochemistry, Vol. 412, Issue. 1-2, p. 155.

    Iskander, George Wood, Kasey Eiffe, Eleanor Yee, Eugene M.H. Black, David StC. and Kumar, Naresh 2015. Ritter reactions of isoflavanols: a facile route to 4-amidoisoflavans. Tetrahedron Letters, Vol. 56, Issue. 15, p. 1941.

    Nie, Qixing Xing, Mengmeng Hu, Jielun Hu, Xiaojuan Nie, Shaoping and Xie, Mingyong 2015. Metabolism and Health Effects of Phyto-estrogens. Critical Reviews in Food Science and Nutrition, p. 00.

    Ahmad, Asif Hayat, Imran Arif, Sara Masud, Tariq Khalid, Nauman and Ahmed, Anwaar 2014. Mechanisms Involved in the Therapeutic Effects of Soybean (Glycine Max). International Journal of Food Properties, Vol. 17, Issue. 6, p. 1332.

    Eiffe, Eleanor Pasquier, Eddy Kavallaris, Maria Herbert, Cristan Black, David StC and Kumar, Naresh 2014. Synthesis, anti-cancer and anti-inflammatory activity of novel 2-substituted isoflavenes. Bioorganic & Medicinal Chemistry, Vol. 22, Issue. 19, p. 5182.

    Fuentes, Eduardo and Palomo, Iván 2014. Antiplatelet effects of natural bioactive compounds by multiple targets: Food and drug interactions. Journal of Functional Foods, Vol. 6, p. 73.

    Zeng, Ai-Guo Pang, Xin-Li Wu, Na Wang, Duan Nan, Guan-Jun Yang, Guang-De and Bian, Xiao-Li 2014. Solubility of daidzein in propylene glycol plus water cosolvent mixtures. Fluid Phase Equilibria, Vol. 366, p. 127.

    Kim, Min Ju and Lim, Yunsook 2013. Protective Effect of Short-Term Genistein Supplementation on the Early Stage in Diabetes-Induced Renal Damage. Mediators of Inflammation, Vol. 2013, p. 1.

    Park, Hyun Jin Jeon, Young Keul You, Dong Hun and Nam, Myeong Jin 2013. Daidzein causes cytochrome c-mediated apoptosis via the Bcl-2 family in human hepatic cancer cells. Food and Chemical Toxicology, Vol. 60, p. 542.

    Chen, Dapeng Xiong, Yongjian Tang, Zeyao Lv, Bochao and Lin, Yuan 2012. Inhibitory effects of daidzein on intestinal motility in normal and high contractile states. Pharmaceutical Biology, Vol. 50, Issue. 12, p. 1561.

    Danciu, CorinaTiulea Soica, Codruta Csanyi, Erzsebet Ambrus, Rita Feflea, Stefana Peev, Camelia and Dehelean, Cristina 2012. Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives. Chemistry Central Journal, Vol. 6, Issue. 1, p. 58.

    Whent, Monica and Slavin, Margaret 2012. Cereals and Pulses.

    Wu, Sheng Hui Shu, Xiao Ou Chow, Wong-Ho Xiang, Yong-Bing Zhang, Xianglan Li, Hong-Lan Cai, Qiuyin Ji, Bu-Tian Cai, Hui Rothman, Nathaniel Gao, Yu-Tang Zheng, Wei and Yang, Gong 2012. Soy Food Intake and Circulating Levels of Inflammatory Markers in Chinese Women. Journal of the Academy of Nutrition and Dietetics, Vol. 112, Issue. 7, p. 996.

    Cena, Emily R. and Steinberg, Francene M. 2011. Soy may help protect against cardiovascular disease. California Agriculture, Vol. 65, Issue. 3, p. 118.

    Cho, Hye Yeon Park, Chung Mu Kim, Mi Jeong Chinzorig, Radnaabazar Cho, Chung Won and Song, Young Sun 2011. Comparative effect of genistein and daidzein on the expression of MCP-1, eNOS, and cell adhesion molecules in TNF-α-stimulated HUVECs. Nutrition Research and Practice, Vol. 5, Issue. 5, p. 381.

    Kawaguchi, Kiichiro Maruyama, Hiroko Hasunuma, Ryoichi and Kumazawa, Yoshio 2011. Suppression of inflammatory responses after onset of collagen-induced arthritis in mice by oral administration of theCitrusflavanone naringin. Immunopharmacology and Immunotoxicology, Vol. 33, Issue. 4, p. 723.

    Lee, Beom-Joon Jo, In-Young Bu, Youngmin Park, Jae-Woo Maeng, Sungho Kang, Hee Jang, Woochang Hwang, Deok-Sang Lee, Wookyoung Min, Kyoungyoon Kim, Jong-In Yoo, Hye Hyun and Lew, Jae-Hwan 2011. Antiplatelet effects of Spatholobus suberectus via inhibition of the glycoprotein IIb/IIIa receptor. Journal of Ethnopharmacology, Vol. 134, Issue. 2, p. 460.

    Park, Eunkyo Lee, Seung Min Jung, In-Kyung Lim, Yunsook and Kim, Jung-Hyun 2011. Effects of genistein on early-stage cutaneous wound healing. Biochemical and Biophysical Research Communications, Vol. 410, Issue. 3, p. 514.

    Szkudelska, Katarzyna Nogowski, Leszek and Szkudelski, Tomasz 2011. Resveratrol and genistein as adenosine triphosphate–depleting agents in fat cells. Metabolism, Vol. 60, Issue. 5, p. 720.


Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function


There has been much recent interest in the cardiovascular benefits of dietary isoflavones. The aim of the present in vitro studies was to investigate potential anti-thrombogenic and anti-atherogenic effects of the isoflavones genistein and daidzein in platelets, macrophages and endothelial cells. Pre-treatment with either isoflavone inhibited collagen-induced platelet aggregation in a dose-dependent manner. In a macrophage cell line (RAW 264·7) activated with interferon γ plus lipopolysaccharide, both isoflavones were found to inhibit NO production and tumour necrosis factor α (TNF-α) secretion dose-dependently, but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo-oxygenase-2. Both isoflavones also dose-dependently decreased monocyte chemoattractant protein-1 secretion induced by TNF-α in human umbilical vein endothelial cells. Compared with daidzein, genistein exerted greater inhibitory effects for all parameters studied. The present data contributes to our knowledge on the molecular mechanisms by which isoflavones may protect against coronary artery disease. Further studies are required to determine whether the effects of isoflavones observed in the current in vitro studies are relevant to the aetiology of coronary artery disease in vivo.

    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function
      Available formats
Corresponding author
*Corresponding Author: Dr Gerald Rimbach, fax +44 118 931 0080, email
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

H Adlercreutz , E Hamalainen & S Gorbach (1993) Dietary phyto-oestrogens and the menopause in Japan. Lancet 342 12091210.

A Arora , MG Nair & GM Strasburg (1998) Antioxidant activities of isoflavones and their biological metabolites in a liposomal system. Archives of Biochemistry and Biophysics 356 133141.

GE Arteel , K Briviba & H Sies (1999) Protection against peroxynitrite by cocoa polyphenol oligomers. FEBS Letters 445 226230.

S Azumi , A Tanimura & K Tanamoto (1997) A novel inhibitor of bacterial endotoxin derived from cinnamon bark. Biochemical and Biophysical Research Communications 234 506510.

CS Baker , RJ Hall , TJ Evans , A Pomerance , J Maclouf , C Creminon , MH Yacoub & JM Polak (1999) Cyclooxygenase-2 is widely expressed in atherosclerotic lesions affecting native and transplanted human coronary arteries and colocalizes with inducible nitric oxide synthase and nitrotyrosine particularly in macrophages. Arteriosclerosis Thrombosis and Vascular Biology 19 646655.

L Boring , J Gosling , M Cleary & IF Charo (1998) Decreased lesion formation in CCR2-/- mice reveals a role for chemokines in the initiation of atherosclerosis. Nature 394 894897.

P Chomczynski & N Sacchi (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Annals of Biochemistry 162 156159.

SJ Duffy , JA Vita , M Holbrook , PL Swerdloff & JF Keaney (2001) Effect of acute and chronic tea consumption on platelet aggregation in patients with coronary artery disease. Arteriosclerosis Thrombosis and Vascular Biology 21 10841089.

GH Gibbons & VJ Dzau (1996) Molecular Therapies For Vascular Diseases. Science 272 689693.

Q Guo , G Rimbach , H Moini , S Weber & L Packer (2002) ESR and cell culture studies on free radical-scavenging and antioxidant activities of isoflavonoids. Toxicology 179 171180.

CL Holder , MI Church well & DR Doerge (1999) Quantification of soy isoflavones, genistein and daidzein, and conjugates in rat blood using LC/ES-MS. Journal of Agricultural and Food Chemistry 47 37643770.

LJ Ignarro (1994) Regulation of cytosolic guanylyl cyclase by porphyrins and metalloporphyrins. Advances in Pharmacology 26 3565.

L Iuliano , JZ Pedersen , D Pratico , G Rotilio & F Violi (1994) Role of hydroxyl radicals in the activation of human platelets. European Journal of Biochemistry 221 695704.

S Kapiotis , N Herrmann , I Held , C Seelos , H Ehringer & BMK Gmeiner (1997) Genistein, the dietary-derived angiogenesis inhibitor, prevents LDL oxidation and protects endothelial cells from damage by atherogenic LDL. Arteriosclerosis Thrombosis and Vascular Biology 17 28682874.

N Kerry & M Abbey (1998) The isoflavone genistein inhibits copper and peroxyl radical mediated low density lipoprotein oxidation in vitro. Atherosclerosis 140 341347.

MC Kowala , R Recce , S Beyer , C Gu & M Valentine (2000) Characterization of atherosclerosis in LDL receptor knockout mice: macrophage accumulation correlates with rapid and sustained expression of aortic MCP-1/JE. Atherosclerosis 149 323330.

R Landolfi , RL Mower & M Steiner (1984) Modification of platelet function and arachidonic acid metabolism by bioflavonoids. Structure-activity relations. Biochemical Pharmacology 33 15251530.

JA Mitchell , S Larkin & TJ Williams (1995) Cyclooxygenase-2: regulation and relevance in inflammation. Biochemical Pharmacology 50 15351542.

NA Nelken , SR Coughlin , D Gordon & JN Wilcox (1991) Monocyte chemoattractant protein-1 in human atheromatous plaques. Journal of Clinical Investigation 88 11211127.

YC Park , G Rimbach , C Saliou , G Valacchi & L Packer (2000) Activity of monomeric, dimeric, and trimeric flavonoids on NO production, TNF-alpha secretion, and NF-kappa B-dependent gene expression in RAW 264·7 macrophages. FEBS Letters 465 9397.

AW Poole & SP Watson (1995) Regulation of cytosolic calcium by collagen in single human platelets. British Journal of Pharmacology 115 101106.

G Rimbach , YP Park , Q Guo , H Moini , C Saliou , K Takayama , F Virgili , F Qureshi & L Packer (2000 a) Nitric oxide synthesis and TNF-alpha secretion in RAW 264·7 macrophages: Mode of action of a fermented papaya preparation. Life Sciences 67 679694.

G Rimbach , G Valacchi , R Canali & F Virgili (2000 b) Macrophages stimulated with IFN-gamma activate NF-kB and induce MCP-1 gene expression in primary human endothelial cells. Molecular and Cell Biology Research Communications 3 238242.

F Sheu , HH Lai & GC Yen (2001) Suppression effect of soy isoflavones on nitric oxide production in RAW 264·7 macrophages. Journal of Agricultural and Food Chemistry 49 17671772.

F Tedesco , F Fischetti , M Pausa , A Dobrina , RB Sim & MR Daha (1999) Complement-endothelial cell interactions: pathophysiological implications. Molecular Immunology 36 261268.

MZ Vitolins , M Anthony & GL Burke (2001) Soy protein isoflavones, lipids and arterial disease. Current Opinions in Lipidology 12 433437.

H Wei , R Bowen , Q Cai , S Barnes & Y Wang (1995) Antioxidant and anti-promotional effects of the soybean isoflavone genistein. Proceedings of the Society of Experimental Biology 208 124130.

S Ylä-Herttuala , BA Lipton , ME Rosenfeld , T Särkioja , T Yoshimura , EJ Leonard , JL Witztum & D Steinberg (1991) Expression of monocyte chemoattractant protein 1 in macrophage-rich areas of human and rabbit atherosclerotic lesions. Proceedings of the National Academy of Sciences, USA 88 52525256.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *