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The effect of ouabain on metabolic rate in guinea-pigs: estimation of energy cost of sodium pump activity

  • R. Swaminathan (a1), Ellen L. P. CHAN (a1), Liu Yuet Sin (a1), Ng Siu King (a1), Ng Sui Fun (a1) and Amy Y. S. Chan (a1)...

Abstract

1. The effect of different doses of ouabain, an inhibitor of the sodium pump, or saline (9 g sodium chloride/1; the vehicle) on the metabolic rate of guinea-pigs weighing 500 g was measured by indirect calorimetry for 120 min.

2. Ouabain (0·02–0·07 nmol/g body-weight) decreased the oxygen consumption in a dose-related manner. When higher doses of ouabain (0·10 nmol/g body-weight or greater) were injected the animals were observed to shiver. Ouabain (0.07 nmol/g body-weight) reduced Na+, K+-ATPase (EC 3. 6. 1. 3) activity of liver, kidney and skeletal muscle by 18·0 (se 6·6), 21.5 (se 6·0) and 21·9 (se 6·8)% respectively. An Eadie-Hofstee plot of percentage decrease in O2 consumption v. percentage inhibition ÷ dose of ouabain showed that maximal inhibition of O2 consumption was 39·4 %.

3. It is concluded that Na pump activity contributed to about 40 % of the resting O2 consumption.

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References

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