Skip to main content
×
×
Home

Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets

  • Min-Tze Liong (a1), Frank R. Dunshea (a2) and Nagendra P. Shah (a3)
Abstract

The aim of this study was to evaluate the effect of a synbiotic containing Lactobacillus acidophilus ATCC 4962, fructooligosaccharide, inulin and mannitol on plasma lipid profiles and erythrocyte membrane properties in hypercholesterolaemic pigs on high- and low-fat diets. Twenty-four white male Landrace pigs were randomly allocated to four treatment groups for 8 weeks (n 6). Treatment factors were the supplementation of synbiotic (with and without) and dietary fat (5 and 15 %). The supplementation of synbiotic reduced plasma total cholesterol (P = 0·001), TAG (P = 0·002) and LDL-cholesterol (P = 0·045) for both dietary fats. A higher concentration of esterified-cholesterol in HDL of pigs supplemented with synbiotic than the control regardless of dietary fat (P = 0·036) indicated that cholesterol was reduced in the form of cholesteryl esters. Reduced concentration of cholesteryl esters (P < 0·001) and increased concentration of TAG (P = 0·042) in LDL of pigs on synbiotic suggested that LDL-cholesterol was reduced via the hydrolysis of smaller and denser LDL particles. The erythrocytes of pigs without any synbiotic showed more prevalence of spur cells than those given the synbiotic, as supported by the higher cholesterol: phospholipid ratio in erythrocytes (P = 0·001). Also, membrane fluidity and rigidity were improved as supported by the decreased fluorescence anisotropies in the Hb-free erythrocyte membrane of pigs given synbiotic (P < 0·001). The administration of the synbiotic reduced plasma TAG, total cholesterol and LDL-cholesterol in hypercholesterolaemic pigs, possibly in the form of cholesteryl esters, via the interrelated pathways of lipid transporters (VLDL, LDL and HDL). The synbiotic also reduced deformation of erythrocytes via improved membrane fluidity and permeability.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Liong Min Tze, fax +604 657 3678, email mintze.liong@usm.my
References
Hide All
Reid, G, Jass, J, Sebulsky, MT & McCormick, JK (2003) Potential uses of probiotics in clinical practice. Clin Microbiol Rev 4, 658672.
Hamilton-Miller, JM (2004) Probiotics and prebiotics for the elderly. Postgrad Med J 80, 447451.
Flickinger, EA, Wolf, BW & Garleb, KA (2000) Glucose-based oligosaccharides exhibit different in vitro fermentation patterns and affect in vivo apparent nutrient digestibility and microbial populations in dogs. J Nutr 130, 12671273.
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125, 14011412.
Kenney, J (2002) Low-fat diets more effective than high-fat diets for both weight loss and health. Pritikin Perspec 11, 18.
Chyou, PH & Eaker, ED (2000) Serum cholesterol concentrations and all-cause mortality in older people. Age Aging 29, 6974.
Liong, MT & Shah, NP (2005) Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. J Dairy Sci 88, 5566.
Liong, MT & Shah, NP (2005) Bile salt deconjugation and BSH activity of five bifidobacterial strains and their cholesterol co-precipitating properties. Food Res Int 38, 135142.
Marlett, JA (1997) Sites and mechanisms for the hypercholesterolemic actions of soluble dietary fibre sources. Adv Exp Med Biol 427, 109121.
Hara, H, Li, S, Sasaki, M, et al. (1994) Effective dose of lactosucrose on faecal flora and faecal metabolites of humans. Biosci Microflora 13, 5163.
Jin, LZ, Ho, YW, Abdullah, N & Jalaludin, S (1998) Growth performance, intestinal microbial populations and serum cholesterol of broiler on diets containing Lactobacillus culture. Poult Sci 77, 12591265.
Lin, SY, Ayres, JW, Winkler, W & Sandine, WE (1989) Lactobacillus effects on cholesterol: in vitro and in vivo results. J Dairy Res 72, 28852899.
Kwiterovich, DO Jr (2000) The metabolic pathways of high-density lipoprotein, low-density lipoprotein, and triacylglycerol: a current review. Am J Cardiol 86, 5L10L.
Cooper, RA, Leslie, MH, Knight, D & Detweiler, K (1980) Red blood cholesterol enrichment and spur cell anemia in dogs fed a cholesterol-enriched, atherogenic diet. J Lipid Res 21, 10821089.
Barshtein, G, Bergelson, L, Dagan, A, Gratton, E & Yedgar, S (1997) Membrane lipid order of human red blood cells is altered by physiological levels of hydrostatic pressure. Am J Physiol 272, H538H543.
Liong, MT & Shah, NP (2005) Optimization of cholesterol removal, growth and fermentation patterns of Lactobacillus acidophilus ATCC 4962 in presence of mannitol, FOS and inulin: a response surface methodology approach. J Appl Microbiol 98, 11151126.
Chapman, MJ, Goldstein, S, Lagrange, D & Lapaud, PM (1981) A density gradient ultracentrifugal procedure for isolation of the major lipoprotein classes from human serum. J Lipid Res 22, 339358.
Duhamel, G, Forgez, P, Nalpas, B, Berthelot, P & Chapman, MJ (1983) Spur cells in patients with alcoholic liver cirrhosis are associated with reduced plasma levels of apoA-II, HDL3, and LDL. J Lipid Res 24, 16121625.
Dumaswala, UJ, Dumaswala, RU, Levin, DS & Greenwalt, TJ (1996) Improved red blood cell preservation correlates with decreased loss of bands 3, 4·1, acetylcholinerase and lipids in microvesicles. Blood 4, 16121616.
Dhaliwal, BS & Steinbrecher, UP (2000) Cholesterol delivered to macrophages by oxidized low density lipoprotein is sequestered in lysosomes and fails to efflux normally. J Lipid Res 41, 16581665.
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265275.
Sardet, C, Hansma, H & Ostwald, R (1972) Characterization of guinea pig plasma lipoproteins: the appearance of new lipoproteins in response to dietary cholesterol. J Lipid Res 13, 624639.
Daly, JA & Ertingshausen, G (1972) Direct method for determining inorganic phosphate in serum with the ‘Centrifichem’. Clin Chem 18, 263265.
Cooper, RA, Leslie, MH, Fischkoff, S, Shinitzky, M & Shattil, SJ (1978) Factors influencing the lipid composition and fluidity of red cell membranes in vitro: production of red cells possessing more than two cholesterols per phospholipid. Biochemistry 17, 327331.
McEneny, J, McMaster, C, Trimble, ER & Young, IS (2002) Rapid isolation of VLDL subfractions: assessment of composition and susceptibility to copper-mediated oxidation. J Lipid Res 43, 824831.
Sanchez-Muniz, FJ, Merinero, MC, Rodríguez-Gil, S, Ordovas, JM, Rodenas, S & Cuesta, C (2002) Dietary fat saturation affects apolipoprotein AII levels and HDL composition in postmenopausal women. J Nutr 132, 5054.
Tholstrup, T, Sandstrom, B, Bysted, A & Holmer, G (2001) Effect of 6 dietary fatty acids on the postprandial lipid profile, plasma fatty acids, lipoprotein lipase, and cholesterol ester transfer activities in healthy young men. Am J Clin Nutr 73, 198208.
Kwiterovich, DO Jr (2002) Lipoprotein heterogeneity: diagnostic and therapeutic implications. Am J Cardiol 90, 1i10i.
Carr, TP, Parks, JS & Rudel, LL (1992) Hepatic ACAT activity in African green monkeys is highly correlated to plasma LDL cholesteryl ester enrichment and coronary artery atherosclerosis. Arterioscler Thromb Vasc Biol 12, 12741283.
Horton, JD, Goldstein, JL & Brown, MS (2002) SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109, 11251131.
Haffner, SM (2002) Lipoprotein disorders associated with type-2 diabetes and insulin resistance. Am J Cardiol 90, 55i61i.
Fernandez, ML, Abdel-Fattah, G & McNamara, DJ (1993) Dietary fat saturation modifies the metabolism of LDL sub-fractions in guinea pigs. Arterioscler Thromb Vasc Biol 13, 14181428.
Rader, DJ (2002) High density lipoproteins and atherosclerosis. Am J Cardiol 90, 62i70i.
Simons, K & Ehehalt, R (2002) Cholesterol, lipid rafts and disease. J Clin Invest 110, 597603.
Kaiser, RD & London, E (1998) Location of duphenylhexatriene (DPH) and its derivatives within membranes: comparison of different fluorescence quenching analyses of membrane depth. Biochemistry 37, 81808190.
Roy, S, Mohanty, A & Dey, J (2005) Microviscosity of bilayer membranes of some N-acylamino acid surfactants determined by fluorescence probe method. Chem Phys Lett 414, 2327.
Sujatha, J & Mishra, AK (1997) Effect of ionic and neutral surfactants on the properties of phospholipid vesicles: investigation using fluorescence probes. J Photochem Photobiol A: Chem 104, 173178.
Ben-Yashar, V & Barenholz, Y (1991) Characterization of the core and surface of human plasma lipoproteins. A study based on the use of five fluorophores. Chem Phys Lipids 60, 114.
Straume, M & Litman, BJ (1988) Equilibrium and dynamic bilayer structural properties of unsaturated acyl chain phosphatidylcholine-cholesterol-rhodopsin recombinant vesicles and rod outer segment disk membranes as determined from higher order analysis of fluorescence anisotropy decay. Biochemistry 27, 77237733.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed