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Effects of epigallocatechin gallate on regulatory T cell number and function in obese v. lean volunteers

  • Jung-Mi Yun (a1), Ishwarlal Jialal (a1) and Sridevi Devaraj (a1)
  • DOI:
  • Published online: 23 February 2010

Obesity predisposes to an increased incidence of diabetes and CVD. Also, obesity is a pro-inflammatory state. Regulatory T cells (Tregs) are essential negative regulators of inflammation and are down-regulated in pro-inflammatory states. Animal models of obesity are associated with decreased Tregs. The dietary modulation of Tregs could be used as a therapeutic strategy to control inflammation. Epigallocatechin gallate (EGCG) is a potent anti-inflammatory agent and an active ingredient of green tea and is suggested to have a role as a preventive agent in obesity, diabetes and CVD. The role of EGCG in the modulation of Tregs has, however, not been studied. Thus, the aim of the present study was to determine the effect of EGCG on the number and function of Tregs in obese and lean human subjects in vitro, and to delineate its specific regulation mechanisms. Tregs were isolated from normal-weight and obese subjects. Tregs were cultured in the absence or presence of EGCG (20 μm) for 24 h. Foxp3-expressing Tregs were enumerated using flow cytometry. Histone deacetylase (HDAC) activity and nuclear NF-κBp65 level were measured by ELISA and Western blots. Obese subjects had lower Tregs and IL-10 production than lean subjects. EGCG treatment significantly enhanced the number of Foxp3-expressing Tregs and IL-10 production in vitro (P < 0·05) in both groups. Also, EGCG decreased NF-κB activity and increased HDAC activity and HDAC-2 expression in Tregs (P < 0·05) in both groups. Thus, in part, EGCG enhances the functionality of Tregs, i.e. IL-10 production and number by suppressing the NF-κB signalling pathway via inducing epigenetic changes.

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*Corresponding author: Dr Sridevi Devaraj, fax +1 91 67346593, email
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1S Yusuf , S Hawken , S Ounpuu , (2004) INTERHEART Study Investigators: effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries (the INTERHEART study): case–control study. Lancet 364, 937952.

2M Visser , LM Bouter , GM McQuillan , (1999) Elevated C-reactive protein levels in overweight and obese adults. JAMA 282, 21312135.

3A O'Garra & P Vieira (2004) Regulatory T cells and mechanisms of immune system control. Nat Med 10, 801805.

4S Sakaguchi (2005) Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and non-self. Nat Immunol 6, 345352.

5TM Brusko , CH Wasserfall , MJ Clare-Salzler , (2005) Functional defects and the influence of age on the frequency of CD4+CD25+ T cells in type 1 diabetes. Diabetes 54, 14071414.

6S Lindley , CM Dayan , A Bishop , (2005) Defective suppressor function in CD4+CD25+ T-cells from patients with type 1 diabetes. Diabetes 54, 9299.

7P Meier , R Meier & E Blanc (2008) Influence of CD4+/CD25+ regulatory T cells on atherosclerosis in patients with end-stage kidney disease. Expert Rev Cadiovasc Ther 6, 987997.

8E Gambineri , TR Torgerson & HD Ochs (2003) Immune dysregulation, polyendocrinopathy, enteropathy, and X-linked inheritance (IPEX), a syndrome of systemic autoimmunity caused by mutations of FOXP3, a critical regulator of T-cell homeostasis. Curr Opin Rheumatol 15, 430435.

10ME Brunkow , EW Jeffery , KA Hjerrild , (2001) Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphopoliferative disorder of the scrufy mouse. Nat Genet 27, 6873.

13K Ito , PJ Barnes & IM Adcock (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1β-induced histone H4 acetylation on lysines 8 and 12. Mol Cell Biol 20, 68916903.

14BP Ashburner , SD Westerheide & AS Baldwin Jr (2001) The p65 (RelA) subunit of NF-κB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression. Mol Cell Biol 21, 70657077.

15S Wolfram (2007) Effects of green tea and EGCG on cardiovascular and metabolic health. J Am Coll Nutr 26, 373S388S.

18MK Levings , R Sangregorio & MG Roncarolo (2001) Human CD25+CD4+T regulatory cells suppress naive and memory T cell proliferation and can be expanded in vitro without loss of function. J Exp Med 193, 12951302.

19EM Shevach (2001) Certified professionals: CD4+CD25+ suppressor T cells. J Exp Med 193, F41F46.

20H Jonuleit , E Schmitt , G Schuler , (2000) Induction of interleukin 10-producing, nonproliferating CD4+ T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells. J Exp Med 192, 12131222.

21S Read & F Powrie (2001) CD4+ regulatory T cells. Curr Opin Immunol 13, 644649.

24SJ Kim , HJ Jeong , KM Lee , (2007) Epigallocatechin-3-gallate suppresses NF-κB activation and phosphorylation of p38 MAPK and JNK in human astrocytoma U373MG cells. J Nutr Biochem 18, 587596.

25A Basu & EA Lucas (2007) Mechanisms and effects of green tea on cardiovascular health. Nutr Rev 65, 361375.

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