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The effects of flavanone-rich citrus juice on cognitive function and cerebral blood flow: an acute, randomised, placebo-controlled cross-over trial in healthy, young adults

  • Daniel J. Lamport (a1), Deepa Pal (a1), Anna L. Macready (a2), Sofia Barbosa-Boucas (a1), John M. Fletcher (a3), Claire M. Williams (a1), Jeremy P. E. Spencer (a2) and Laurie T. Butler (a1)...
Abstract
Abstract

A plausible mechanism underlying flavonoid-associated cognitive effects is increased cerebral blood flow (CBF). However, behavioural and CBF effects following flavanone-rich juice consumption have not been explored. The aim of this study was to investigate whether consumption of flavanone-rich juice is associated with acute cognitive benefits and increased regional CBF in healthy, young adults. An acute, single-blind, randomised, cross-over design was applied with two 500-ml drink conditions – high-flavanone (HF; 70·5 mg) drink and an energy-, and vitamin C- matched, zero-flavanone control. A total of twenty-four healthy young adults aged 18–30 years underwent cognitive testing at baseline and 2-h after drink consumption. A further sixteen, healthy, young adults were recruited for functional MRI assessment, whereby CBF was measured with arterial spin labelling during conscious resting state at baseline as well as 2 and 5 h after drink consumption. The HF drink was associated with significantly increased regional perfusion in the inferior and middle right frontal gyrus at 2 h relative to baseline and the control drink. In addition, the HF drink was associated with significantly improved performance on the Digit Symbol Substitution Test at 2 h relative to baseline and the control drink, but no effects were observed on any other behavioural cognitive tests. These results demonstrate that consumption of flavanone-rich citrus juice in quantities commonly consumed can acutely enhance blood flow to the brain in healthy, young adults. However, further studies are required to establish a direct causal link between increased CBF and enhanced behavioural outcomes following citrus juice ingestion.

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Corresponding author
* Corresponding author: Professor L. T. Butler, email l.t.butler@reading.ac.uk
References
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1. Spencer JPE (2010) Beyond antioxidants: the cellular and molecular interactions of flavonoids and how these underpin their actions on the brain. Proc Nutr Soc 69, 244260.
2. Menze ET, Tadros MG, Abdel-Tawed AM, et al. (2012) Potential neuroprotective effects of hesperidin on 3-nitropropuinic acid-induced neurotoxicity in rats. Neurotoxicology 33, 12651275.
3. Rendeiro C, Vauzour D, Rattray M, et al. (2013) Dietary levels of pure flavonoids improve spatial memory performance and increase hippocampal brain derived neurotrophic factor. PLOS ONE 8, e63535.
4. Laske C, Stellos K, Hoffmann N, et al. (2011) Higher BDNF serum levels predict slower cognitive decline in Alzheimer’s disease patients. Int J Neuropsychopharmacol 14, 399404.
5. Butchart C, Kyle J, McNeill G, et al. (2011) Flavonoid intake in relation to cognitive function in later life in the Lothian Birth Cohort 1936. Br J Nutr 106, 141148.
6. Lamport DJ, Dye L, Wightman JD, et al. (2012) The effects of flavonoid and other polyphenol consumption on cognitive performance: a systematic research review of human experimental and epidemiological studies. Nutr Aging 1, 525.
7. Lamport DJ, Hoyle E, Lawton CL, et al. (2011) Evidence for a second meal cognitive effect: glycaemic responses to high and low glycaemic index meals are associated with cognition the following morning. Nutr Neurosci 14, 6671.
8. Krikorian R, Nash TA, Shidler MD, et al. (2010) Concord grape juice supplementation improves memory function in older adults with mild cognitive impairment. Br J Nutr 103, 730734.
9. Krikorian R, Boespflug EL, Fleck DE, et al. (2012) Concord grape juice supplementation and neurocognitive function in human aging. J Agric Food Chem 60, 57365742.
10. Kean RJ, Lamport DJ, Dodd GF, et al. (2015) Chronic consumption of flavanone-rich orange juice is associated with cognitive benefits: an 8-week randomised double-blind placebo-controlled trial in healthy older adults. Am J Clin Nutr 101, 506514.
11. Schroeter H, Heiss C, Balzer J, et al. (2006) (-)-Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans. Proc Natl Acad Sci U S A 103, 10241029.
12. Francis S, Head K, Morris PG, et al. (2006) The effect of flavanol-rich cocoa on the fMRI response to a cognitive task in healthy young people. J Cardiovasc Pharmacol 47, S215S220.
13. Brickman AM, Khan US, Provenzano FA, et al. (2014) Enhancing dentate gyrus function with dietary flavanols improves cognition in older adults. Nat NeuroSci 17, 17981803.
14. Camfield DA, Scholey A, Pipingas A, et al. (2012) Steady state visually evoked potential (SSVEP) topography changes associated with cocoa flavanol consumption. Physiol Behav 105, 948957.
15. Krikorian R, Shidler MD, Nash TA, et al. (2010) Blueberry supplementation improves memory in older adults. J Agric Food Chem 58, 39964000.
16. Lamport DJ, Saunders C, Butler LT, et al. (2014) Fruits, vegetables, 100% juices and cognitive function. Nutr Rev 72, 774789.
17. Bach M (1996) The Freiburg Visual Acuity Test – automatic measurement of visual acuity. Optom Vis Sci 73, 4953.
18. Stadler MA (1995) Role of attention in implicit learning. J Exp Psychol Learn 21, 674685.
19. Wechsler D (1981) The psychometric tradition – developing the Wechsler adult intelligence scale. Contemp Educ Psychol 6, 8285.
20. Stroop JR (1935) Studies of interference in serial verbal reactions. J Exp Psychol 18, 643662.
21. Morris N & Jones DM (1990) Memory updating in working memory: the role of the central executive. Br J Psychol 81, 111121.
22. Garavan H, Ross TJ & Stein EA (1999) Right hemispheric dominance of inhibitory control: an event-related functional MRI study. Proc Natl Acad Sci U S A 96, 83018306.
23. Binder JR, Frost JA, Hammeke TA, et al. (1999) Conceptual processing during the conscious resting state: a functional MRI study. J Cogn Neurosci 11, 8093.
24. Aron AR, Robbins TW & Poldrack RA (2004) Inhibition and the right inferior frontal cortex. Trends Cogn Sci 8, 170177.
25. Lamport DJ, Pal D, Moutsiana C, et al. (2015) The effect of flavanol rich cocoa on cerebral perfusion in healthy older adults during conscious resting state: a placebo controlled crossover acute trial. Psychopharmacology (Berl) 232, 32273234.
26. Berdt DS, Hwang PM & Snyder SH (1990) Localization of nitric oxide synthesis indicating a neural role for nitric oxide. Nature 347, 768770.
27. Toda N, Ayajiki K & Okamura T (2009) Cerebral blood flow regulation by nitric oxide in neurological disorders. Can J Physiol Pharmacol 87, 581594.
28. Loke WM, Hodgson JM, Proudfoot JM, et al. (2008) Pure dietary flavonoids quercetin and (-)-epicatechin augment nitric oxide products and reduce endothelin-1 acutely in healthy men. Am J Clin Nutr 88, 10181025.
29. Rizza S, Muniyappa R, Lantorno M, et al. (2011) Citrus polyphenol hesperidin stimulates production of nitric oxide in endothelial cells while improving endothelial function and reducing inflammatory markers in patients with metabolic syndrome. J Clin Endocrinol Metab 96, E782E792.
30. Bondonno CP, Downey LA, Croft KD, et al. (2014) The acute effect of flavonoid-rich apples and nitrate rich spinach on cognitive performance and mood in healthy men and women. Food Funct 5, 849858.
31. Field DT, Williams CM & Butler LT (2011) Consumption of cocoa flavanols results in an acute improvement in visual and cognitive functions. Physiol Behav 103, 255260.
32. Scholey A, French SJ, Morris PJ, et al. (2010) Consumption of cocoa flavanols results in acute improvements in mood and cognitive performance during sustained mental effort. J Psychopharmacol 24, 15051514.
33. Manach C, Scalbert A & Morand C (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79, 2747.
34. Mullen W, Archeveque MA, Edwards CA, et al. (2008) Bioavailability and metabolism of orange juice flavanones in humans: impact of a full-fat yogurt. J Agric Food Chem 56, 1115711164.
35. Alharbi MH, Lamport DJ, Dodd G, et al. (2016) Flavonoid rich orange juice is associated with acute improvements in cognitive function in healthy middle aged males. Eur J Nutr 55, 20212029.
36. Youdim KA, Dobbie MS, Kuhnle G, et al. (2003) Interaction between flavonoids and the blood-brain barrier: in vitro studies. J Neurochem 85, 180192.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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