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Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis

Published online by Cambridge University Press:  09 March 2007

Sung-Ling Yeh ,
Yu-Ni Lai ,
Huey-Fang Shang ,
Ming-Tsan Lin  and
Wei-Jao Chen
Sung-Ling Yeh
Affiliation:
Institute of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Yu-Ni Lai
Affiliation:
Institute of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Huey-Fang Shang
Affiliation:
Department of Surgery, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
Ming-Tsan Lin
Affiliation:
Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan, Republic of China
Wei-Jao Chen*
Affiliation:
Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan, Republic of China
*
*Corresponding author: Dr Wei-Jao Chen, fax +886 2 2341 2969, email chenwj@ccms.ntu.ed.tw
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Abstract

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The present study examined the effect of glutamine (Gln)-enriched diets before sepsis or Gln-containing total parenteral nutrition (TPN) after sepsis, or both, on the phagocytic activity and blood lymphocyte subpopulation in rats with gut-derived sepsis. Rats were assigned to a control group or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet; groups 3 and 4 had part of casein replaced by Gln. After feeding the diets for 10d, sepsis was induced by caecal ligation and puncture (CLP); TPN was maintained for 3d after CLP. The control group and groups 1 and 3 were infused with conventional TPN and groups 2 and 4 were supplemented with Gln in the TPN solution. All rats were killed 3d after CLP or sham operation to examine their immune responses. The results showed that compared with the control group, the phagocytic activities of peritoneal macrophages were enhanced in groups 3 and 4, but not in groups 1 and 2. The proportion of CD3+ cells in group 1 was significantly lower (P<0·05) than that of the control group, whereas no differences were observed among the control and Gln-supplemented groups. The CD4+ cell proportion was significantly lower (P<0·05) in group 1 compared with the control group and groups 3 and 4. These findings suggest that Gln-enriched diets before CLP significantly enhanced peritoneal macrophage phagocytic activity, preserved CD4+ cells and maintained blood total T lymphocytes in gut-derived sepsis. However, parenteral Gln administration after caecal ligation and puncture had no favourable effects on modulating immune response in septic rats.

Keywords

GlutamineGut-derived sepsisPhagocytosisLymphocyte subpopulation
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 3 , March 2004 , pp. 423 - 429
Copyright
Copyright © The Nutrition Society 2004

References

Alverdy, JA, Aoys, E, Weiss-Carrington, P & Burke, DA (1992) The effect of glutamine-enriched TPN on gut immune cellularity. J Surg Res 52, 34–38.CrossRefGoogle ScholarPubMed
Ardawi, MS (1992) Effects of epidermal growth factor and glutamine-supplemented parenteral nutrition on the small bowel of septic rats. Clin Sci 82, 573–580.Google ScholarPubMed
Askanazi, J, Carpentier, YA, Michelson, CB, Elwyn, DH, Furst, P, Kantrowitz, LR, Gump, FE & Kinney, JM (1980) Muscle and plasma amino acids following injury. Influence of intercurrent infection. Ann Surg 192, 78–85.Google ScholarPubMed
Böhmer, RH, Trinkle, LS & Staneck, JL (1992) Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxidative burst. Cytometry 13, 525–531.CrossRefGoogle Scholar
Calder, PC (1994) Glutamine and the immune system. Clin Nutr 13, 2–8.CrossRefGoogle ScholarPubMed
DiPiro, JT (1997) Cytokine networks with infection: mycobacterial infections, leishmaniasis, human immunodeficiency virus infection, and sepsis. Pharmacotherapy 17, 205–223.CrossRefGoogle Scholar
Furukawa, S, Saito, H, Inoue, T, Matsuda, T, Fukutsu, K, Han, I, Ikeda, S & Hidemura, A (2000) Supplemental glutamine augments phagocytosis and reactive oxygen intermediate production by neutrophils and monocytes from postoperative patients in vitro. Nutrition 16, 323–329.CrossRefGoogle ScholarPubMed
Gardiner, K & Barbul, A (1993) Intestinal amino acid absorption during sepsis. J Parenter Ent Nutr 17, 277–283.CrossRefGoogle ScholarPubMed
Gardiner, KR, Gardiner, RE & Barbul, A (1995) Reduced intestinal absorption of arginine during sepsis. Crit Care Med 23, 1227–1232.CrossRefGoogle ScholarPubMed
Gianotti, L, Alexander, JW, Gennari, R, Pyles, T & Babcock, GF (1995) Oral glutamine decreases bacterial translocation and improves survival in experimental gut-origin sepsis. J Parenter Ent Nutr 19, 69–74.CrossRefGoogle ScholarPubMed
Gismondo, MR, Drago, L, Fassina, MC, Vaghi, I, Abbiati, R & Grossi, E (1998) Immunostimulating effect of oral glutamine. Dig Dis Sci 43, 1752–1754.CrossRefGoogle ScholarPubMed
Hammerquist, F, Wernerman, J, Al, R, Vonder, D & Vinners, E (1989) Addition of glutamine to total parenteral nutrition after elective abdominal surgery spares glutamine in muscle, counteracts the fall in muscle protein synthesis and improves nitrogen balance. Ann Surg 209, 455–461.CrossRefGoogle Scholar
Heberer, M, Babst, R, Juretic, A, Gross, T, Horig, H, Harder, F & Spagnoli, GC (1996) Role of glutamine in the immune response in critical illness. Nutrition 12, Suppl., S71–S72.CrossRefGoogle ScholarPubMed
Kew, S, Wells, SM, Yaqoob, P, Wallace, FA, Miles, EA & Calder, PC (1999) Dietary glutamine enhances murine T-lymphocyte responsiveness. J Nutr 129, 1524–1531.CrossRefGoogle ScholarPubMed
Kudsk, KA, Wu, Y, Fukatsu, K, Zarzaur, BL, Johnson, CD, Wang, R & Hanna, MK (2000) Glutamine-enriched total parenteral nutrition maintains intestinal interleukin-4 and mucosal immunoglobulin A levels. J Parenter Ent Nutr 24, 270–275.CrossRefGoogle ScholarPubMed
Lacey, JM & Wilmore, DW (1990) Is glutamine a conditionally essential amino acid? Nutr Rev 48, 297–309.CrossRefGoogle ScholarPubMed
Matsukawa, A, Hogaboam, CM, Lukacs, NW, Lincoln, PM, Evanoff, HL & Kunkel, SL (2000) Pivotal role of the CC chemokine, macrophage-derived chemokine, in the innate immune response. J Immunology 164, 5362–5368.CrossRefGoogle Scholar
Naka, S, Saito, H, Hashiguchi, Y, Lin, MT, Furukawa, S, Inaba, T, Fukushima, R, Wada, N & Muto, T (1996) Alanyl-glutamine-supplemented total parenteral nutrition improves survival and protein metabolism in rat protracted bacterial peritonitis model. J Parenter Ent Nutr 20, 417–423.CrossRefGoogle ScholarPubMed
Navaratnam, RL, Morris, SE, Traber, DL, Flynn, J, Woodson, L & Linares Herndon, DN (1990) Endotoxin (LPS) increases mesenteric vascular resistance (MVR) and bacterial translocation (BT). J Trauma 30, 1104–1113.CrossRefGoogle ScholarPubMed
Ogle, CK, Ogle, JD, Mao, JX, Simon, J, Noel, JG, Li, BG & Alexander, JW (1994) Effect of glutamine on phagocytosis and bacterial killing in normal and pediatric burn patient neutrophils. J Parenter Ent Nutr 18, 128–133.CrossRefGoogle ScholarPubMed
Parronchi, P, DeCarli, M, Manetti, R, Simonelli, C, Sampognaro, S, Piccinni, MP, Macchia, D, Maggi, E, Del Prete, G & Romagnani, S (1992) IL-4 and IFN (alpha and gamma) exert opposite regulatory effects on the development of cytolytic potential of Th1 or Th2 human T cell clones. J Immunol 149, 2977–2983.CrossRefGoogle ScholarPubMed
Parry-Billings, M, Evans, J, Calder, PC & Newsholme, EA (1990) Does glutamine contribute to immunosuppression after major burns? Lancet 336, 523–525.CrossRefGoogle ScholarPubMed
Ringer, TV & Zimmermann, JJ (1992) Inflammatory host responses in sepsis. Crit Care Clinics 8, 163–189.Google Scholar
Rohde, T, Maclean, DA & Klarlund Pedersen, B (1996) Glutamine, lymphocyte proliferation and cytokine production. Scand J Immunol 44, 648–650.CrossRefGoogle ScholarPubMed
Roth, E, Funovis, J, Muhlbacher, F, Schemper, M, Mauritz, W, Sporn, P & Fritsch, A (1982) Metabolic disorders in severe abdominal sepsis: glutamine deficiency in skeletal muscle. Clin Nutr 1, 25–28.CrossRefGoogle ScholarPubMed
Sawyer, DW, Donowitz, DR & Mandell, GL (1989) Polymorphonuclear neutrophils: an effective antimicrobial force. Rev Infect Dis 11, 1532–1544.CrossRefGoogle ScholarPubMed
Schiffrin, EJ, Rochat, F, Link-Amster, H, Aeschlimann, JM & Donnet-Hughes, A (1995) Immunomodulation of human blood cells following ingestion of lactic acid bacteria. J Dairy Sci 78, 491–497.CrossRefGoogle ScholarPubMed
Shou, J & Daly, JM (1994) Enteral glutamine augments host immune function. Surg Forum 45, 33–34.Google Scholar
Smith, RJ & Panico, K (1985) Automated analysis of o-phthalaldehyde derivatives of amino acids in physiological fluids of reverse phase high performance liquid chromatography. J Liq Chromatogr 8, 1783–1795.CrossRefGoogle Scholar
Smith, RJ & Willmore, DW (1990) Glutamine nutrition and requirements. J Parenter Ent Nutr 14, 94S–99S.CrossRefGoogle ScholarPubMed
Souba, WW (1991) Glutamine: a key substrate for the splanchnic bed. Annu Rev Nutr 11, 285–308.CrossRefGoogle ScholarPubMed
Wells, SM, Kew, S, Yaqoob, P, Wallace, FA & Calder, PC (1999) Dietary glutamine enhances cytokine production by murine macrophages. Nutrition 15, 881–884.CrossRefGoogle ScholarPubMed
Wichterman, KA, Baue, AE & Chaudry, IH (1980) Sepsis and septic shock – a review of laboratory models and a proposal. J Surg Res 29, 189–201.CrossRefGoogle ScholarPubMed
Wilmore, DW & Shabert, JK (1998) Role of glutamine in immunologic responses. Nutrition 14, 618–626.CrossRefGoogle ScholarPubMed
Wischmeyer, PE, Kahana, M, Wolfson, R, Ren, H, Musch, MM & Chang, EB (2001) Glutamine reduces cytokine release, organ damage, and mortality in a rat model of endotoxemia. Shock 16, 398–402.CrossRefGoogle Scholar
Yeh, SL, Yeh, CL, Lin, MT, Lo, PN & Chen, WJ (2001) Effects of glutamine-supplemented total parenteral nutrition on cytokine production and T cell population in septic rats. J Parenter Ent Nutr 25, 269–274.Google Scholar
Zellweger, R, Ayala, ADeMaso, CMChaudry, IH (1995) Trauma-hemorrhage causes prolonged depression in cellular immunity. Shock 4, 149–153.CrossRefGoogle ScholarPubMed