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Effects of inulin on faecal bifidobacteria in human subjects

  • Hans-P. Kruse (a1), Brigitta Kleessen (a2) and Michael Blaut (a2)

A controlled study with eight healthy free-living subjects was carried out, in which energy intake was adjusted to the individual energy requirements. On administration of inulin, blood lipids, the faecal microflora, short-chain fatty acids and accompanying gastrointestinal symptoms were characterized in order to investigate the long-term effect of inulin. During the run-in phase (8 d), subjects received a typical Western diet providing 45 % energy as fat and 40 % energy as carbohydrate. Subsequently, the subjects consumed a fat-reduced diet which provided 30 % energy as fat and 55 % energy as carbohydrate for a period of 64 d using inulin as a fat replacer. The amounts of inulin consumed by the subjects (up to 34 g/d) were based on individual energy requirements with the aim to keep the diet isoenergetic with that used in the run-in period. To assess the effects of inulin administration, a control study (run-in and intervention) was carried out in which subjects consumed the same diet but devoid of inulin during the whole course of the study. To investigate the effect of inulin on faecal flora composition total bacteria and bifidobacteria in the faeces were enumerated by in situ hybridization with 16S rRNA targeted oligonucleotide probes. Inulin significantly increased bifidobacteria from 9·8 to 11·0 log10/g dry faeces and caused a moderate increase in gastrointestinal symptoms such as flatulence and bloatedness, whereas blood lipids and short-chain fatty acids remained essentially unaffected.

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Corresponding author
*Corresponding author: Dr Hans-P. Kruse, fax +49 33 2008 8573, email
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AM Coulston , GC Liu & GM Reaven (1983) Plasma glucose, insulin and lipid responses to high-carbohydrate low-fat diets in normal humans. Metabolism 32, 5256.

JH Cummings (1981) Short-chain fatty acids in the human colon. Gut 22, 763779.

JH Cummings & GT MacFarlane (1997) Role of intestinal bacteria in nutrient metabolism. Clinical Nutrition 16, 311.

MH Davidson , KC Maki , C Synecki , SA Torri & KB Drennan (1998) Effects of dietary inulin on serum lipids in men and women with hypercholesterolemia. Nutrition Research 18, 503517.

R Fuller (1991) Probiotics in medicine. Gut 32, 439442.

GR Gibson & X Wang (1994) Regulatory effects of bifidobacteria on other colonic bacteria. Journal of Applied Bacteriology 77, 412420.

SM Grundy (1991) Recent nutrition research: implications for foods of the future. Annals of Medicine 23, 187193.

H Hidaka , Y Tashiro & T Eida (1991) Proliferation of bifidobacteria by oligosaccharides and their useful effect on human health. Bifidobacteria Microflora 10, 6579.

A Pedersen , B Sandström & JMM van Amselvoort (1997) The effect of ingestion of inulin on blood lipids and gastrointestinal symptoms in healthy females. British Journal of Nutrition 78, 215222.

EW Pomare , WJ Branch & JH Cummings (1985) Carbohydrate fermentation in the human colon and its relation to acetate concentrations in venous blood. Journal of Clinical Investigation 75, 14481454.

MB Roberfroid (1993) Dietary fiber, inulin and oligofructose: a review comparing their physiological effects. Critical Reviews in Food Science and Nutrition 33, 102148.

MB Roberfroid , GR Gibson & N Delzenne (1993) The biochemistry of oligofructose, a nondigestible fibre: an approach to calculate its caloric value. Nutrition Reviews 51, 137146.

WEW Roediger (1980) Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut 21, 793798.

IR Roland (1988) Factors affecting metabolic activity of the intestinal microflora. Drug Metabolism Reviews 19, 243261.

W Scheppach (1994) Effects of short chain fatty acids on gut morphology and function. Gut 35, S35S38.

L Sobotka , M Bratova , M Slemrova , J Manak , J Vizda & Z Zadak (1997) Inulin as the soluble fiber in liquid enteral nutrition. Nutrition 13, 2125.

J Steiniger , H Karst , R Noack & H-D Steglich (1987) Diet-induced thermogenesis in man: thermic effects of single protein and carbohydrate test meals in lean and obese subjects. Annals of Nutrition and Metabolism 31, 117125.

X Wang & GR Gibson (1993) Effect of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75, 373380.

GF Watts , B Lewis , JNH Brunt , ES Lewis , DJ Coltart , LDR Smith , JI Mann & AV Swan (1992) Effects on coronary artery disease of lipid-lowering diet, or diet plus cholestyramine, in the St Thomas' Atherosclerosis Regression Study (STARS). Lancet 339, 563569.

GW Welling , P Elfferich , GC Raangs , ACM Wildeboer-Veloo , GJ Jansen & JE Degener (1997) 16S Ribosomal rRNA-targeted oligonucleotide probes for monitoring of intestinal tract bacteria. Scandinavian Journal of Gastroenterology 32, Suppl. 222, 1719.

K Yamashita , K Kawai & M Itakura (1984) Effects of fructo-oligosaccharides on blood glucose and serum lipids in diabetic subjects. Nutrition Research 4, 961966.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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