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Effects of inulin-type fructans on lipid metabolism in man and in animal models

Published online by Cambridge University Press:  08 March 2007

Michel Beylot*
Inserm U 499, Faculté RTH Laennec, Rue G Paradin, Lyon, 69008, France
*Corresponding author: Dr Michel Beylot, fax +33 4 78778739, email
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Studies in rodents show that inulin and oligofructose can reduce the plasma levels of cholesterol and triacylglycerols (TG). In addition, they can oppose TG accumulation in liver and have favourable effects on hepatic steatosis. The hypotriglyceridaemic effect is due to a reduction in hepatic re-esterification of fatty acids, but mainly in the expression and activity of liver lipogenesis, resulting in lower hepatic secretion rate of TG. This repression of lipogenesis is not observed in adipose tissue. The effect on liver lipogenesis can be explained by reduced insulin/glucose levels or by a selective exposure of the liver to increased amounts of propionic acid produced in the large intestine during fermentation of non-digestible carbohydrates. The decrease in plasma cholesterol could also be due to inhibition of cholesterol synthesis by propionic acid or to modifications in the bile acid metabolism. Studies in man yield more conflicting results with a decrease or no effects on plasma lipid levels, and, when a decrease is observed, more marked effects on TG than on cholesterol and more consistent action of inulin than of oligofructose. Besides the difference in the dose of inulin or oligofructose used, differences in metabolic status could play a role in this discrepancy between man and animals since reduction in plasma TG is observed in man mainly in a situation of increased liver lipogenesis (high-carbohydrate diet, obesity, hypertriglyceridaemia). The effects on plasma cholesterol appear also more marked in hyperlipidaemic subjects than in healthy controls, suggesting that inulin and oligofructose have beneficial effects in these types of subjects.

Research Article
Copyright © The Nutrition Society 2005


Aghelli, N, Kabir, M, Berni-Canani, S, Petijean, E, Boussairi, A, Luo, J, Bornet, F, Slama, G & Rikzalla, S (1998) Plasma lipids and fatty acid synthase activity are regulated by short-chain fructo-oligosaccharides in sucrose-fed insulin-resistant rats. J Nutr 128, 12831288.CrossRefGoogle Scholar
Alles, M, De Roos, N, Baks, J, Van de Lisdonk, E, Zock, P, Hautvast, J (1999) Consumption of fructooligosaccharides does not favorably affect blood glucose and serum lipid concentrations in patients with type 2 diabetes. Am J Clin Nutr 69, 6469.CrossRefGoogle Scholar
Balcazar-Munoz, B, Martinez-Abindis, E, Gonzalez-Ortiz, M (2003) Effect of oral inulin administration on lipid profile and insulin sensitivity in subjects with obesity and dyslipidemia. Rev Med Chil 131, 597604.Google Scholar
Brass, E & Vetter, W (1994) Interleukin-6, but not TNF-alpha, increases lipogenesis in rat hepatocyte primary cultures. Biochem J 301, 193197.CrossRefGoogle Scholar
Brighenti, F, Casiraghi, M, Canzi, E & Ferrari, A (1999) Effect of consumption of a ready-to-eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers. Eur J Clin Nutr 53, 726733.CrossRefGoogle ScholarPubMed
Busserolles, J, Gueux, E, Rock, E, Demigne, C, Mazur, A & Rayssiguier, Y (2003) Oligofructose protects against the hypertriglyceridemic and prooxidative effects of a high fructose diet in rats. J Nutr 133, 19031908.CrossRefGoogle ScholarPubMed
Causey, J, Feirtag, J, Gallagher, D, Tingland, B & Slavin, J (2000) Effect of dietary inulin on serum lipids, blood glucose and the gastrointestinal environment in hypercholesterolemic men. Nutr Res 20, 191201.CrossRefGoogle Scholar
Daubioul, C, Taper, H, De Wispelaere, L & Delzenne, N (2000) Dietary oligofructose lessens hepatic steatosis, but does not prevent hypertriglyceridemia in obese Zucker rats. J Nutr 130, 13141319.CrossRefGoogle Scholar
Daubioul, C, Rousseau, N, Demeure, R, Gallez, B, Taper, H, Declerk, B & Delzenne, N (2002) Dietary fructans, but not cellulose, decrease triglyceride accumulation in the liver of obese Zucker fa/fa rats. J Nutr 132, 967973.CrossRefGoogle Scholar
Davidson, M & Maki, K (1999) Effects of dietary inulin on serum lipids. J Nutr 129, 1474S1477S.CrossRefGoogle ScholarPubMed
Davidson, M, Maki, K, Synecki, C, Torri, S & Drennan, K (1998) Effects of dietary inulin on serum lipids in men and women with hypercholesterolemia. Nutr Res 18, 503517.CrossRefGoogle Scholar
Delzenne, N & Daubioul, C (2000) Dietary fructans and lipid metabolism: building a bridge from the colon to the liver. Recent Res Dev Nutr 3, 227238.Google Scholar
Delzenne, N & Kok, N (1999) Biochemical basis of oligofructose-induced hypolipidemia in animal models. J Nutr 129, 1467S1470S.CrossRefGoogle ScholarPubMed
Delzenne, N & Kok, N (2001) Effects of fructans-type prebiotics on lipid metabolism. Am J Clin Nutr 73, 456S458S.CrossRefGoogle ScholarPubMed
Delzenne, N & Williams, C (2002) Prebiotics and lipid metabolism. Curr Opin Lipidol 13, 6167.CrossRefGoogle ScholarPubMed
Delzenne, N, Kok, N, Fiordaliso, M, Deboyser, D, Goethals, F & Roberfroid, M (1993) Dietary fructooligosaccharides modify lipid metabolism in rats. Am J Clin Nutr 57 820S.CrossRefGoogle Scholar
Demigné, G, Morand, C, Levrat, M, Besson, C, Moundras, C, Rémésy, C (1995) Effect of propionate on fatty acids and cholesterol synthesis and on acetate metabolism in isolated rat hepatocytes. Br J Nutr 74, 209219.CrossRefGoogle ScholarPubMed
Diraison, F & Beylot, M (1998) Role of human liver lipogenesis and reesterification in triglycerides secretion and in FFA reesterification. Am J Physiol 274, E321E327.Google ScholarPubMed
Diraison, F, Dusserre, E, Vidal, H, Sothier, M & Beylot, M (2002) Increased hepatic lipogenesis but decreased expression of lipogenic gene in adipose tissue in human obesity. Am J Physiol 282, E46E51.Google ScholarPubMed
Ebert, R, Nauck, M & Creutzfeldt, W (1991) Effect of exogenous and endogenous GIP on plasma triglycerides responses in rats. Horm Metab Res 23, 517521.CrossRefGoogle ScholarPubMed
Ellegärd, L, Andersson, H & Bosaeus, L (1997) Inulin and oligofructose do not influence the absorption of cholesterol, or the excretion of cholesterol, Ca, Mg, Zn, Fe, or bile acids but increases energy excretion in ileostomy subjects. Eur J Clin Nutr 51, 15.CrossRefGoogle ScholarPubMed
Fernandez, M (2001) Soluble fiber and nondigestible carbohydrate effects on plasma lipids and cardiovascular risk. Curr Opin Lipidol 12, 3540.CrossRefGoogle ScholarPubMed
Fiordaliso, M, Kok, N, Desager, F, Goethals, F, Deboyer, D, Roberfroid, M & Delzenne, N (1995) Oligofructose supplemented diet lowers serum and VLDL concentrations of triglycerides, phospholipids and cholesterol in rats. Lipids 30, 163167.CrossRefGoogle Scholar
Flickinger, E, Van Loo, J & Fahey, G (2003) Nutritional responses to the presence of inulin and oligofructose in the diets of domesticated animals: a review. Crit Rev Food Sci Nutr 43, 1960.CrossRefGoogle Scholar
Forcheron, F, Cachefo, A, Thevenon, S, Pinteur, C & Beylot, M (2002) Mechanisms of the triglyceride and cholesterol-lowering effect of fenofibrate in hyperlipidemic type 2 diabetic patients. Diabetes 51, 34863491.CrossRefGoogle ScholarPubMed
Foufelle, F, Ferré, P (2002) New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: a role for the transcription factor sterol regulatory element binding protein-1c. Biochem J 366, 377391.CrossRefGoogle ScholarPubMed
Girard, J, Perdereau, P, Foufelle, F, Prip-Buus, C & Ferre, P (1994) Regulation of lipogenic enzyme genes expression by nutrients and hormones. FASEB J 8, 3642.CrossRefGoogle ScholarPubMed
Hidaka, H, Tashiro, Y & Eida, T (1991) Proliferation of bifidobacteria by oligosaccharides and their useful effect on human health. Bifidobacteria Microflora 10, 6579.CrossRefGoogle Scholar
Hokanson, J & Austin, M (1996) Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of population based prospective studies. J Cardiovasc Risk 3, 213219.CrossRefGoogle Scholar
Hudgins, LC, Hellerstein, MK, Seidman, C, Neese, R, Diakun, J & Hirsh, J (1996) Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet. J Clin Invest 98, 20812091.CrossRefGoogle Scholar
Jackson, K, Taylor, G, Clohessy, A & Williams, C (1999) The effect of the daily intake of inulin on fasting lipid, insulin, and glucose concentrations in middle-aged men and women. Br J Nutr 82, 2330.CrossRefGoogle ScholarPubMed
Jones, PJ (1997) Regulation of cholesterol biosynthesis by diet in humans. Am J Clin Nutr 66, 438446.CrossRefGoogle ScholarPubMed
Joseph, S, Laffitte, B, Patel, P, Watson, M, Matsukuma, K, Walczak, R, Collins, J, Osborne, T & Tononoz, P (2002) Direct and indirect mechanisms for regulation of fatty acid synthase gene expression by liver X receptors. J Biol Chem 277, 1101911025.CrossRefGoogle ScholarPubMed
Klag, M, Ford, D & Mead, L (1993) Serum cholesterol in young men and subsequent cardiovascular disease. N Engl J Med 328, 313318.CrossRefGoogle ScholarPubMed
Knapper, J, Puddicombe, S, Morgan, L & Fletcher, J (1995) Investigations into the actions of GIP and GLP-1 (7-36) amide on lipoprotein lipase activity in explants of rat adipose tissue. J Nutr 125, 183188.Google Scholar
Kok, N, Roberfroid, M & Delzenne, N (1996a) Dietary oligofructose modifies the impact of fructose on hepatic triacylglycerol metabolism. Metabolism 45, 15471550.CrossRefGoogle ScholarPubMed
Kok, N, Roberfroid, M, Robert, A & Delzenne, N (1996b) Involvement of lipogenesis in the lower VLDL secretion induced by oligofructose in rats. Br J Nutr 76, 881990.CrossRefGoogle ScholarPubMed
Kok, N, Morgan, L, Williams, C, Roberfroid, M, Thissen, J & Delzenne, N (1998a) Insulin, GLP-1, GIP and IGF-1 as putative mediators of the hypolipidemic effect of oligofructose in rats. J Nutr 128, 10991103.CrossRefGoogle ScholarPubMed
Kok, N, Taper, H & Delzenne, N (1998b) Oligofructose modulates lipid metabolism alterations induced by a fat-rich diet in rats. J Appl Toxicol 18, 4753.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
Lawler, J, Yin, M, Diehl, A, Roberts, E & Chaterjee, S (1998) TNF-alpha stimulates the maturation of SREBP-1 in human hepatocytes through the action of neutral shingomyelinase. J Biol Chem 273, 50535059.CrossRefGoogle Scholar
Letexier, D, Diraison, F & Beylot, M (2003a) Addition of inulin to a high carbohydrate diet reduces hepatic lipogenesis and plasma triacylglycerol concentration in humans. Am J Clin Nutr 77, 559564.CrossRefGoogle ScholarPubMed
Letexier, D, Pinteur, C, Large, V, Frering, V & Beylot, M (2003b) Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue. J Lipid Res 44, 21272134.CrossRefGoogle ScholarPubMed
Levrat, M, Favier, M, Moundras, C, Remesy, C, Démigné, C & Morand, C (1994) Role of dietary propionic acid and bile excretion in the hypocholesterolemic effects of oligosaccharides in rats. J Nutr 124, 531538.CrossRefGoogle ScholarPubMed
Luo, J, Van Yperselle, M, Rizkalla, S, Rossi, F, Bornet, F & Slama, G (2000) Chronic consumption of short-chain fructooligosaccharides does not affect basal hepatic glucose production or insulin-resistance in type 2 diabetes. J Nutr 130, 15721577.CrossRefGoogle Scholar
Luo, L, Rizkalla, S & Alamowitch, C (1996) Chronic consumption of short-chain fructooligosaccharides by healthy subjects decreased basal hepatic glucose production but had no effect on insulin-stimulated glucose metabolism. Am J Clin Nutr 63, 939945.CrossRefGoogle ScholarPubMed
Nishina, P & Freeland, R (1990) Effects of propionate on lipidbiosynthesis in isolated rat hepatocytes. J Nutr 120, 669673.CrossRefGoogle Scholar
Oben, J, Morgan, L, Fletcher, L & Marks, V (1991) Effect of the entero-pancreatic hormones GIP and GLP-1 (7-36) amide on fatty acid synthesis in explants of rat adipose tissue. J Endocrinol 130, 267272.CrossRefGoogle ScholarPubMed
Pedersen, A, Sandström, B & Van Amelsvoort, J (1997) The effect of ingestion of inulin on blood lipids and gastrointestinal symptoms in healthy females. Br J Nutr 78, 215222.CrossRefGoogle ScholarPubMed
Roberfroid, M (1993) Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. Crit Rev Food Sci Nutr 33, 103148.CrossRefGoogle ScholarPubMed
Schneeman, B (1999) Fiber, inulin and oligofructose: similarities and differences. J Nutr 129, 1424S1427S.CrossRefGoogle ScholarPubMed
Trautwein, E, Rieckhoff, D & Erbersdobler, H (1998) Dietary inulin lowers plasma cholesterol and triacylglycerols and alters biliary bile acid profile in hamsters. J Nutr 128, 19371943.CrossRefGoogle ScholarPubMed
Uyeda, K, Yamashita, H & Kawaguchi, T (2002) Carbohydrate responsive element-binding protein (ChREBP): a key regulator of glucose metabolism and fat storage. Biochem Pharmacol 63, 20752080.CrossRefGoogle ScholarPubMed
Van Dokum, W, Wesendonk, B, Srikumar, T, Van den Heuvel, E (1999) Effect of nondigestible oligosaccharides on large-bowel functions, blood lipid concentrations and glucose absorption in young healthy male subjects. Eur J Clin Nutr 53, 17.CrossRefGoogle Scholar
West of Scotland Coronary revention Study Group (1998) Influence of pravastatin and plasma lipids on clinical events in the West of Scotland Coronary Prevention Group. Circulation 97, 14401445.CrossRefGoogle Scholar
Wigg, A, Roberts-Thomson, I, Dymock, R, McCarthy, P, Grose, R & Cummins, A (2001) The role of small intestinal bacterial overgrowth, intestinal permeability, endotoxaemia, and TNF-alpha in the pathogenesis of non-alcoholic steatohepatitis. Gut 48, 206211.CrossRefGoogle ScholarPubMed
Wolever, T, Spadafora, P, Cunnane, S & Pencharz, P (1995) Propionate inhibits incorporation of colonic (1,2-13C2) acetate into plasma lipids in humans. Am J Clin Nutr 61, 12411247.CrossRefGoogle Scholar
Wright, R, Anderson, J & Briges, S (1990) Propionate inhibits hepatocytes lipids synthesis. Proc Soc Exp Biol Med 195, 2629.CrossRefGoogle Scholar
Yamashita, K, Kawai, K & Itakura, M (1984) Effects of fructo-oligosaccharides on blood glucose and serum lipids in diabetic subjects. Nutr Res 4, 961966.CrossRefGoogle Scholar
Zanpelas, A, Morgan, L, Furlonger, N & Williams, C (1995) Effects of dietary fatty acid composition on basal and hormone-stimulated hepatic lipogenesis and on circulating lipids in rats. Br J Nutr 74, 381392.CrossRefGoogle Scholar