Skip to main content Accessibility help
×
×
Home

The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse

  • Felix O. Omara (a1) and Barry R. Blakley (a1)

Abstract

The influence of Fe status on cell-mediated immunity was studied in weanling mice fed on Fe-deficient (7 mg Fe/kg), Fe-sufficient (120 mg Fe/kg) and high-Fe (3000 or 5000 mg Fe/kg) diets for 7 weeks. The contact sensitivity (CS) response to dinitrofluorobenzene (DNFB), the in vivo delayed-type hypersensitivity (DTH) response to sheep erythrocytes (SRBC) and the ability of primed spleen cells to transfer DTH response to naive normal mice were suppressed in mice consuming the Fe-deficient diet. High-Fe diets (3000 or 5000 mg Fe/kg) selectively suppressed the CS response to DNFB, but the DTH response to SRBC or the transfer of DTH response by primed spleen cells to naive normal mice remained normal. Spleen cell functions associated with the expression of class II major histocompatibility (MHC) surface antigens, concanavalin A-induced interleukin-2 (IL-2) secretion or the antigen-presenting cell (APC) ability to stimulate antigen-dependent proliferation of an SRBC-specific helper T-lymphocyte clone were not altered by Fe status. However, consistent with the suppressed DTH response in the Fe- deficient mice was the suppressed concanavalin A-induced T-lymphocyte blastogenesis and the interferon-γ (INF-γ) production by spleen cells from mice fed on the Fe-deficient diet. Spleen cells from mice fed on excess levels of Fe in the diet secreted less INF-γ than the control mice, although T- lymphocyte proliferation remained unaffected. Suppression of the cellular immune response associated with Fe deficiency may be related in part to impaired T-lymphocyte proliferation and INF-γ secretion rather than to deficits in IL-2 secretion or APC function.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The effects of iron deficiency and iron overload on cell-mediated immunity in the mouse
      Available formats
      ×

Copyright

References

Hide All
Abbas, A. K., Lichtman, A. H. & Pober, J. S. (1991) Cellular and Molecular Immunology. Toronto: W. B. Saunders Company.
Bhan, A. K., Perry, L. L., Cantor, H., McCluskey, R. T., Benacerraf, B. & Greene, M. I. (1981) The role of T cell sets in the rejection of methylcholanthrene-induced sarcoma (S1509a) in syngeneic mice. American Journal of Pathology 102, 2027.
Bhaskaram, P., Sharada, K., Sivakumar, B., Rao, K. V. & Nair, M. (1989) Effects of iron and vitamin A deficiency on macrophage functions in children. Nutrition Research 9, 3545.
Blakley, B. R. & Hamilton, D. L. (1988) The effect of iron deficiency on the immune response in mice. Drug-Nutrient Interactions 5, 249–255. Bretscher, P. A. (1979) In vitro induction of delayed-type hypersensitivity. European Journal of Immunology 9, 311316.
Chandra, R. K. (1975) Impaired immunocompetence associated with iron deficiency. Journal of Paediatrics 86, 899902.
Dovrak, H. F., Galli, S. J. & Dovrak, A. M. (1986) Cellular and vascular manifestations of cell-mediated immunity. Human Pathology 17, 122137.
Dwyer, J., Wood, C., McNamara, J., Williams, A, Andiman, W., Rink, L., O'Conner, T. & Pearson, H. (1987) Abnormalities in immune system of children with beta thalassemia major. Clinical and Experimental Immunology 68, 621629.
Flesch, I. & Kaufmann, S. H. E. (1987) Mycobacteria growth inhibition of interferon-r-activated bone marrow macrophages and differential susceptibility among strains of Mycobacterium tuberculosis. Journal of Immunology 138, 44084413.
Heinzel, F. P., Sadick, M. D., Holaday, B. J., Coffman, R. M. & Locksley, J. (1989) Reciprocal expression of interferon-r or interleukin 4 during the resolution or progression of murine leishmaniasis: evidence for expansion of distinct helper T cell subsets. Journal of Experimental Medicine 169, 5972.
Joynson, D. H. M., Walker, D. M., Jacobs, A. & Dolby, A. E. (1972) Defect of cell mediated immunity of patients with iron deficiency anemia. Lancet ii, 10581059.
Kuvibidila, S. R., Baliga, B. S. & Suskind, R. M. (1981) Effects of iron deficiency anemia on delayed cutaneous hypersensitivity in mice. American Journal of Clinical Nutrition 34, 26352640.
MacDougall, L. G., Anderson, R., McNab, G. M. & Katz, J. (1975) The immune response in iron-deficient children: impaired cellular defense mechanisms with lateral humoral components. Journal of Paediatrics 86, 833843.
Menard, S., Colnaghi, M. & Cornalba, G. (1973) Immunogenicity and immunosensitivity of urethan-induced murine lung adenomata in relation to the host immunological impairment of the primary tumour host. British Journal of' Cancer 27, 345350.
Omara, F. O. & Blakley, B. R. (1993). Influence of low dietary iron and iron overload on urethan-induced lung tumours in mice. Canadian Journal of Veterinary Research 57, 209211.
Ray, M. C., Tharp, M. D., Sullivan, R. J. & Tigelaar, R. E. (1983) Contact sensitivity reactions to dinitrofluorobenzene mediated by monoclonal IgE anti-DNP antibodies. Journal of Immunology 131, 10961102.
Smith, K. A. (1984) Interleukin 2. Annual Review of Immunology 2, 319333.
Sorbie, J. & Valberg, L. S. (1975) Iron balance in the mouse. Laboratory Animal Science 24, 900904.
Spear, A. T. & Sherman, A. R. (1992) Iron deficiency alters DMBA-induced tumor burden and natural killer cell cytotoxicity in rats. Journal of Nutrition 122, 4655.
Thorson, J. A, Smith, K. M., Gomez, F., Naumann, P. W., & Kemp, J. D. (1991) Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG mAbs against the transferrin receptor and the iron chelator desferrioxamine. Cellular Immunology 124, 126137.
Vadas, M. A, Miller, J. F., A., P., Gamble, J. & Whitelaw, A. A. (1975) A radioisotopic method to measure delayed type hypersensitivity in the mouse. International Archives of Allergy and Applied Immunology 49, 7092.
Van Asbeck, B. S., Vergrugh, H. A, Van, Oost, B. A., Marx, J. J. M., Imhof, H. & Verhoef, J. (1982) Lbteriu monocytogenes meningitis and decreased phagocytosis associated with iron overload. British Medical Journal 284, 542544.
Van Eijk, H. H. & de Jong, G. (1992) The physiology of iron, transferrin and ferritin. Biological Trace Element Research 35, 1324.
Weaver, C. T. & Unanue, E. R. (1990) The costimulatory function of antigen presenting cells. Immunology Today 11. 4955.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed