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Effects of Lactobacillus acidophilus NCFM on insulin sensitivity and the systemic inflammatory response in human subjects

  • Anne Sofie Andreasen (a1), Nadja Larsen (a2), Theis Pedersen-Skovsgaard (a1), Ronan M. G. Berg (a1), Kirsten Møller (a1) (a3), Kira Dynnes Svendsen (a4), Mogens Jakobsen (a2) and Bente Klarlund Pedersen (a1)...
Abstract

According to animal studies, intake of probiotic bacteria may improve glucose homeostasis. We hypothesised that probiotic bacteria improve insulin sensitivity by attenuating systemic inflammation. Therefore, the effects of oral supplementation with the probiotic bacterium Lactobacillus acidophilus NCFM on insulin sensitivity and the inflammatory response were investigated in subjects with normal or impaired insulin sensitivity. In a double-blinded, randomised fashion, forty-five males with type 2 diabetes, impaired or normal glucose tolerance were enrolled and allocated to a 4-week treatment course with either L. acidophilus NCFM or placebo. L. acidophilus was detected in stool samples by denaturating gradient gel electrophoresis and real-time PCR. Separated by the 4-week intervention period, two hyperinsulinaemic–euglycaemic clamps were performed to estimate insulin sensitivity. Furthermore, the systemic inflammatory response was evaluated by subjecting the participants to Escherichia coli lipopolysaccharide injection (0·3 ng/kg) before and after the treatment course. L. acidophilus NCFM was detected in 75 % of the faecal samples after treatment with the probiotic bacterium. Insulin sensitivity was preserved among volunteers in the L. acidophilus NCFM group, whereas it decreased in the placebo group. Both baseline inflammatory markers and the systemic inflammatory response were, however, unaffected by the intervention. In conclusion, intake of L. acidophilus NCFM for 4 weeks preserved insulin sensitivity compared with placebo, but did not affect the systemic inflammatory response.

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Corresponding author
*Corresponding author: Dr Anne Sofie Andreasen, fax +45 3545 7644, email sofie_andreasen@msn.com
References
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1Yadav H, Jain S & Sinha PR (2008) The effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei on gastropathic consequences in diabetic rats. J Med Food 11, 6268.
2Yadav H, Jain S & Sinha PR (2007) Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutrition 23, 6268.
3Yadav H, Jain S & Sinha PR (2008) Oral administration of dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei delayed the progression of streptozotocin-induced diabetes in rats. J Dairy Res 75, 189195.
4Guarner F & Schaafsma GJ (1998) Probiotics. Int J Food Microbiol 39, 237238.
5Food and Agriculture Organization & World Health Organization (2001) Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Geneva: WHO.
6Marco ML, Pavan S & Kleerebezem M (2006) Towards understanding molecular modes of probiotic action. Curr Opin Biotechnol 17, 204210.
7Jacobsen CN, Rosenfeldt Nielsen V, Hayford AE, et al. (1999) Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl Environ Microbiol 65, 49494956.
8Cani PD & Delzenne NM (2009) The role of the gut microbiota in energy metabolism and metabolic disease. Curr Pharm Des 15, 15461558.
9Turnbaugh PJ, Ley RE, Mahowald MA, et al. (2006) An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444, 10271031.
10Jia W, Li H, Zhao L, et al. (2008) Gut microbiota: a potential new territory for drug targeting. Nat Rev Drug Discov 7, 123129.
11Walker WA (2008) Mechanisms of action of probiotics. Clin Infect Dis 46, Suppl. 2, S87S91.
12Rescigno M, Urbano M, Valzasina B, et al. (2001) Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nat Immunol 2, 361367.
13Guarner F & Malagelada JR (2003) Gut flora in health and disease. Lancet 361, 512519.
14Madsen K, Cornish A, Soper P, et al. (2001) Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology 121, 580591.
15Cani PD, Neyrinck AM, Fava F, et al. (2007) Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia. Diabetologia 50, 23742383.
16Guha M & Mackman N (2001) LPS induction of gene expression in human monocytes. Cell Signal 13, 8594.
17Pickup JC, Mattock MB, Chusney GD, et al. (1997) NIDDM as a disease of the innate immune system: association of acute-phase reactants and interleukin-6 with metabolic syndrome X. Diabetologia 40, 12861292.
18Pickup JC (2004) Inflammation and activated innate immunity in the pathogenesis of type 2 diabetes. Diabetes Care 27, 813823.
19Shoelson SE, Lee J & Goldfine AB (2006) Inflammation and insulin resistance. J Clin Invest 116, 17931801.
20Konstantinov SR, Smidt H, de Vos VM, et al. (2008) S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions. Proc Natl Acad Sci U S A 105, 1947419479.
21Chen CC, Chiu CH, Lin TY, et al. (2008) Effect of probiotics L. acidophilus on C. rodentium colitis: the role of dendritic cells. Pediatr Res (epublication ahead of print version 17 September 2008).
22Ouwehand AC, Tiihonen K, Saarinen M, et al. (2009) Influence of a combination of Lactobacillus acidophilus NCFM and lactitol on healthy elderly: intestinal and immune parameters. Br J Nutr 101, 367375.
23Sanders ME & Klaenhammer TR (2001) Invited review: the scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic. J Dairy Sci 84, 319331.
24Altermann E, Russell WM, Azcarate-Peril MA, et al. (2005) Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proc Natl Acad Sci U S A 102, 39063912.
25DeFronzo RA, Tobin JD & Andres R (1979) Glucose clamp technique: a method for quantifying insulin secretion and resistance. Am J Physiol 237, E214E223.
26Andreasen AS, Krabbe KS, Krogh-Madsen R, et al. (2008) Human endotoxemia as a model of systemic inflammation. Curr Med Chem 15, 16971705.
27World Health Organization (2006) Definitions and Diagnosis of Diabetes Mellitus and Intermediate Hyperglycemia. Report of a WHO/IDF consultation. Geneva: WHO.
28Walter J, Hertel C, Tannock GW, et al. (2001) Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67, 25782585.
29Muyzer G, de Waal EC & Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695700.
30ben Omar N & Ampe F (2000) Microbial community dynamics during production of the Mexican fermented maize dough pozol. Appl Environ Microbiol 66, 36643673.
31Ferrannini E & Mari A (1998) How to measure insulin sensitivity. J Hypertens 16, 895906.
32Brehm A & Roden M (2009) Glucose clamp techniques. In Clinical Diabetes Research, Methods and Techniques, pp. 4367 [Roden M, editor]. Chichester: John Wiley and Sons Ltd.
33Taudorf S, Krabbe KS, Berg RM, et al. (2007) Human models of low-grade inflammation: bolus versus continuous infusion of endotoxin. Clin Vaccine Immunol 14, 250255.
34Andreasen AS, Pedersen-Skovsgaard T, Mortensen OH, et al. (2009) The effect of glutamine infusion on the inflammatory response and HSP70 during human experimental endotoxaemia. Crit Care 13, R7.
35Andreasen AS, Pedersen-Skovsgaard T, Berg RMG, et al. (2009) Type 2 diabetes is associated with impaired cytokine response and adhesion molecule expression in human endotoxaemia. Intensive Care Med (epublication ahead of print version 13 March 2010).
36Membrez M, Blancher F, Jaquet M, et al. (2008) Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice. FASEB J 22, 24162426.
37Brugman S, Klatter FA, Visser JT, et al. (2006) Antibiotic treatment partially protects against type 1 diabetes in the Bio-Breeding diabetes-prone rat. Is the gut flora involved in the development of type 1 diabetes? Diabetologia 49, 21052108.
38Ma X, Hua J & Li Z (2008) Probiotics improve high-fat diet-induced hepatic steatosis and insulin resistance by increasing hepatic NKT cells. J Hepatol 49, 821830.
39Diaz-Ropero MP, Martin R, Sierra S, et al. (2007) Two Lactobacillus strains, isolated from breast milk, differently modulate the immune response. J Appl Microbiol 102, 337343.
40Hotamisligil GS, Arner P, Caro JF, et al. (1995) Increased adipose tissue expression of tumor necrosis factor-α in human obesity and insulin resistance. J Clin Invest 95, 24092415.
41Fink LN, Zeuthen LH, Ferlazzo G, et al. (2007) Human antigen-presenting cells respond differently to gut-derived probiotic bacteria but mediate similar strain-dependent NK and T cell activation. FEMS Immunol Med Microbiol 51, 535546.
42Sato M, Uzu K, Yoshida T, et al. (2008) Effects of milk fermented by Lactobacillus gasseri SBT2055 on adipocyte size in rats. Br J Nutr 99, 10131017.
43Backhed F, Manchester JK, Semenkovich CF, et al. (2007) Mechanisms underlying the resistance to diet-induced obesity in germ-free mice. Proc Natl Acad Sci U S A 104, 979984.
44Backhed F, Ding H, Wang T, et al. (2004) The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A 101, 1571815723.
45Hotamisligil GS (2006) Inflammation and metabolic disorders. Nature 444, 860867.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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