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Effects of mimosine administered to a perfused area of skin in Angora goats

  • R. Puchala (a1), S. G. Pierzynowski (a1), T. Sahlu (a1) and S. P. Hart (a1)
Abstract

The effect of mimosine on a perfused area of skin tissue was studied using an isolated perfusion technique. Four mature Angora wethers (body weight 35 (SE 2·3) kg) were cannulated bilaterally with indwelling silicone catheters in the superficial branches of the deep circumflex iliac artery and vein. Mimosine (40 mg/kg metabolic weight (W0·75) per d) was infused intra-arterially into one iliac artery of each goat for 3 d and saline was infused in the contralateral (control) iliac artery. Iliac venous blood samples were taken from both sides along with arterial samples from the carotid artery. Mimosine infusion elevated plasma mimosine in the carotid artery (52·6 (SEM 19·21)µmol/I) and iliac vein on the saline-treated side to 54·1 (SEM 16·31)µ/I and in the iliac vein on the mimosine-treated side to 191·3 (SEM19·14) µmol/I (P < 0·01). Mimosine decreased feed intake (2·3 v. 0·6 kg/d, SEM 0·29; P < 0·001) and water consumption (5·2 v. 1·3 litres/d, SEM 0·67; P < 0·001). Mimosine did not cause defleecing in the area of infusion and was cleared from the bloodstream within 12 h of cessation of infusion. The following effects were also observed during mimosine infusion: decrease in plasma amino acids to half pre-infusion values (methionine 22·7 v. 13·1 µmol/l, SEM 1·41; lysine 95·9 p. 37·4 µmol/l, SEM 4·28; P < 0·001); decreases in plasma triiodothyronine (1495 v. 695 ng/l, SEM 43·1; P < 0·001), thyroxine (61·5 v. 19·5 µg/l, SEM 1·8; P < 0·001) and insulin (28·7 v. 17·3 µIU/ml, SEM 1·89; P < 0·01) concentrations; increase in plasma cortisol (14 v. 62 µg/l, SEM 0·35; P < 0·001) concentration; decreases in levels of plasma Zn and Mg (0·97. v. 0·49 mg/l, SEM 0·063; P < 0·001 and 21·4 v. 14·6 mg/l, SEM 1·74; P < 0·001 respectively). All reported variables returned to their normal values 24 h after cessation of mimosine infusion except feed intake which was affected for a longer period. Mohair length and diameter were not affected by mimosine infusion. The toxicity of mimosine may be due to the drastic depletion of Zn and Mg in the blood as mimosine possesses very strong chelating properties and is excreted in the urine as a chelate.

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References
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American Society for Testing and Materials (1988). Standard test method D2130. Wool content of raw wool, laboratory scale. Annual Book of ASTM Standards, pp. 335365. Philadelphia: American Society for Testing and Materials.
Brock, J. H., Liceaga, J. & Kontoghiorghes, G. J. (1988). The effect of synthetic iron chelators on bacterial growth in human serum. Microbiology and Immunology 1, 5560.
Couinaud, C. (1984). Le zinc. Journal of Chiropractic, Paris 121, 611621.
Crounse, R. G., Maxwell, J. D. & Blank, H. (1962). Inhibition of growth of hair by mimosine. Nature 194, 694695.
Depasquale-Jardieu, P. & Fraker, P. J. (1979). The role of corticosterone in the loss in immune function in the zinc-deficient A/J mouse. Journal of Nutrition 109, 18471855.
El-Harith, E. A., Hiller, A. & Meulen, U. (1983). The effect of administration of glycine and tyrosine on the growth depression caused by mimosine in rats. Journal of Animal Physiology and Animal Nutrition 50, 132137.
El-Harith, E. A., Mohme, H., Meulen, U., Bartha, M. & Gunther, K. D. (1981). Effects of mimosine on some serum enzyme activities and amino acid metabolism in the rat. Journal of Animal Physiology and Animal Nutrition 46, 255263.
Fernandez, J. M., Sahlu, T., Lu, C. D. & Akinsoyinu, A. O. (1991). Mimosine administration in goats: effects on lactational performance and metabolism in early Alpine lactating does. Journal of Animal Science 69, Suppl. 1, 392 Abstr.
Forsbeck, K., Nilsson, K. & Kontoghiorghes, G. J. (1987). Variation in iron accumulation, transferrin membrane binding and DNA synthesis in the K-562 and U-937 cell lines induced by chelators and their iron complexes. European Journal of Haematology 39, 318325.
Franzolin, N. R. & Velloso, L. (1986). Leucaena leucocephala (Lam. ) de Wit em racoes para ovinos. 2. Toxicidade (Leucaena leucocephala (Lam.) in diets for sheep. 2. Toxicity). Revista da Sociedade Brasileira de Zootecnia 15, 415424.
Hayden, J. M., Williams, J. E. & Collier, R. J. (1993). Plasma growth hormone, insulin-like growth factor, insulin, and thyroid hormone association with body protein and fat accretion in steers undergoing compensatory gain after dietary energy restriction. Journal of Animal Science 77 33273338
Hegarty, M. P. (1978). Toxic amino acids of plant origin. In Eflects of Poisonous Plants on Livestock, pp. 575585 [Keller, R. F.van Kampen, K. R. and Lynn, J. F. editors]. New York: Academic Press.
Hegarty, M. P., Lee, C. P., Christie, G. S., De Munk, F. G. & Court, R. D. (1978). Comparative toxicities of mimosine and some chemically related compounds to mouse bone marrow cells in liquid culture. Australian Journal of Biological Sciences 31, 2740.
Hoey, W. A. & Hopkins, P. S. (1983). Chronic arterial cannulation for studying the skin of sheep. Research in Veterinary Science 35, 247249.
Jacquemet, N., Fernandez, J. M., Sahlu, T. & Lu, C. D. (1990). Mohair quality and metabolic profile of Angora goats during acute minosine toxicity. Journal of Animal Science 68, Suppl. 1, 400 Abstr.
Jhala, U. S. & Baly, D. L. (1991). Zinc deficiency results in a post transcriptional impairment in insulin synthesis. FASEB Journal 5, A941 Abstr.
Jones, R. J. & Hegarty, M. P. (1984). The effect of different proportions of Leucaena leucocephala in the diet of cattle on growth, feed intake, thyroid function and urinary excretion of dihydroxypyridine. Australian Journal of Agricultural Research 35, 317320.
Kontoghiorghes, G. J. (1990). Chelators affecting iron absorption in mice. Arzneumittelforschung 40, 13321335.
Lamand, M., Lab, C., Mignon, M. & Tressol, J. C. (1983). A zinc-deficient diet for ruminants: diagnosis and treatment of deficiency. Annals de Recherches Vétérinaires 4, 211215.
McLaren, G. D., Muir, W. A. & Kellermeyer, R. W. (1983). Iron overload disorders: natural history, pathogenesis, diagnosis, and therapy. Critical Reviews in Clinical and Laboratory Sciences 19, 205266.
Masters, D. G. & Moir, R. J. (1983). Effect of Zinc deficiency on the pregnant ewe and developing foetus. British Journal of Nutrition 49, 365372.
Mosca, P. J., Dijkwel, P. A. & Hamlin, J. L. (1992). The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells. Molecular and Cellular Biology 12, 43754383.
Muhlbauer, B. & Osswald, H. (1992). Feeding but not salt loading is the dominant factor controlling urinary dopamine excretion in conscious rats. Naunyn Schmiedebergs Archiv Pharmacology 364, 469471.
Norton, P., Falciglia, G. & Gist, D. (1993). Physiologic control of food intake by neural and chemical mechanisms. Journal of the American Dietetic Association 93, 450457.
Pierzynowski, S. G., Sahlu, T., Puchala, R., Hart, S. P. & Al-Dehneh, A. (1994). Local infusion of glucose and insulin in isolated skin perfusion sites in Angora goats. Small Ruminant Research 14, 137141.
Prasad, J. (1989). A note on toxic effects of Leucaena leucocephala in goats: a clinical study. Indian Journal of Veterinary Medicine 9, 151152.
Puchala, R., Pierzynowski, S. G., Sahlu, T. & Hart, S. P. (1995 a). Effects of amino acids administered to a perfused area of the skin in Angora goats. Journal of Animal Science 73, 565570.
Puchafa, R., Sahlu, T., Davis, J. J. & Hart, S. P. (1995 b). Influence of mineral supplementation on 2,3-dihydroxypyridine toxicity in Angora goats. Journal of Animal Science 73, 565570.
Reis, P. J. (1978). Effectiveness of intravenous and abomasal doses of mimosine for defleecing sheep and effects on subsequent wool growth. Australian Journal of Agricultural Researcy, 10431055.
Reis, P. J. & Sahlu, T. (1994). The nutritional control of the growth and properties of mohair and wool fibers: a comparative review. Journal of Animal Science 72, 18991910.
Reisner, A. H., Bucholtz, C. A. & Ward, K. A. (1979). Effect of the plant amino acid mimosine on cell division, DNA, RNA and protein synthesis in Paramection. Molecular Pharmacology 16, 278286.
Roth, H. P. & Kirchgessner, M. (1981). Zinc and insulin metabolism. Biological Trace Element Research 3, 1332.
Sahlu, T., Fernandez, J. M., Lu, C. D. & Manning, R. (1992). Dietary protein level and ruminal degradability for mohair production in Angora goats. Journal of Animal Science 70, 15261533.
Schmid, H. (1988). Untersuchungen zum toxikologischen Wirkungsmechanismus von Mimosin (Investigations into the toxicological mechanisms of mimosine). Inaugural-Dissertation. Tierarztliche Fakultat der Ludwig-Maximilians-Universitat, München, Germany.
Seeley, R. C. & Kinsey, W. J. (1982). The Determination ojTrace Elements in Whole Blood by DC Plasma Emission Spectroscopy. Haverhill, MA: Spectra Metrics/Beckman Instruments.
Statistical Analysis Systems (1985). User's Guide: Statistics. Cary, NC: SAS Institute Inc.
Steel, R. G. D. & Torrie, J. A. (1980). Principles and Procedures of Statistics. New York: McGraw-Hill Book CO. Inc.
Sunaria, K. R. & Vidya-Sagar, V. (1989). Subabul (Leucuenu leucocephala) leaf meal – its chemical composition, amino acid make up and detoxification of mimosine and tannins. Indian Journal of Animal Nutrition 6, 223226.
Szyszka, M. & Meulen, U. (1984). Die Bestimmung der biologischen Halbwertszeit für Mimosin von Ratten (The measurement of the biological half-life of mimosine in rats). Zeitschrift Tierarztliche Wochemchrift 91, 262264.
Szyszka, M. & Meulen, U. (1985). Das Verhalten von Schafen gegenüber dem toxischen Mimosin in Leucaena leucocephala (The behavioural response of sheep to the toxin mimosine in Leucaena leucocephala). Journal of Animal Physiology and Animal Nutrition 53, 6569.
Tangendjaja, B. & Willis, R. B. H. (1980). Analysis of mimosine and 3-hydroxy-4(1H)-pyridone by high-performance liquid chromatography. Journal of Chromatography 202, 317318.
Thompson, J. F., Morris, C. L, & El-Harith, E. A. (1969). The naturally occurring amino acids. Review in Biochemistry 38, 137158.
Tsai, W. C. (1961). The effect of mimosine on several enzymes. Formosan Medical Association 60, 5864.
Unterhalt, B. (1980). Toxische Aminosauren und Proteine in Pflanzen (Toxic amino acids and proteins in plants). Deutsche Apotheker Zeitung 24, 10931094.
Ward, K. A. & Harris, R. L. N. (1976). Inhibition of wool follicle DNA synthesis by mimosine and related 4(1H)-phridones. Australine Journal of Biological Sciences 29, 189196.
Watson, P. A., Hanauske-Abel, H. H., Flint, A. & Lalande, M. (1991). Mimosine reversibly arrests cell cycle progression at the G1-S phase border. Cytometry 12, 242246.
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British Journal of Nutrition
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