Hostname: page-component-7479d7b7d-68ccn Total loading time: 0 Render date: 2024-07-14T23:41:51.289Z Has data issue: false hasContentIssue false

Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism and blood flow in dietary hypoferric anaemic mice

Published online by Cambridge University Press:  09 March 2007

N. Manabe*
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
Y. Azuma
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
M. Sugimoto
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
K. Uchio
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
M. Miyamoto
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
N. Taketomo
Affiliation:
Meiji Institute of Health Science, Meiji Milk Products Co. Ltd, Odawara 250-0862, Japan
H. Tsuchita
Affiliation:
Nutrition Science Institute, Meiji Milk Products Co. Ltd, Tokyo 189-8530, Japan
H. Miyamoto
Affiliation:
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
*
*Corresponding author: Dr Noboru Manabe, fax +81 75 753 6345, email manabe@jkans.jkans.kais.kyoto-u.ac.jp
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The beneficial effects of a traditional Chinese medicine, Cordyceps sinensis (Cs), on mice with hypoferric anaemia were evaluated by NMR spectroscopy. Experimental hypoferric anaemia was induced in mice by feeding with an Fe-free diet for 6 weeks. They were then given extract from cultured Cs (200 mg/kg body weight daily, orally) and were placed on an Fe-containing recovery diet (35 mg Fe/kg diet) for 4 weeks. In vivo31P and 2H NMR spectra acquired noninvasively and quantitatively at weekly intervals were used to evaluate hepatic energy metabolism and blood flow in the mice. During the 4-week Cs-extract treatment, consistent increases were observed in liver β-ATP: inorganic phosphate value by liver 31P NMR spectroscopy, representing the high energy state, and in blood-flow rate as determined by 2H NMR spectroscopy of deuterated water (D2O) uptake after intravenous injection of D2O. The haematological variables (the packed cell volume and the haemoglobin level) and the hepatic intracellular pH, which was determined from the NMR chemical shift difference between the inorganic phosphate peak and the α-phosphate peak of ATP, were not significantly different between Cs-extract-treated and control mice. As blood flow and energy metabolism are thought to be linked, the Cs-extract-increased hepatic energy metabolism in the dietary hypoferric anaemic mice was concluded to be due to increased hepatic blood flow.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2000

References

Azuma, Y, Manabe, N, Kawai, F, Kanamori, M and Miyamoto, H (1994) Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rats. Journal of Animal Science 72, 103108.CrossRefGoogle ScholarPubMed
Bates, TE, Williams, SR, Busza, AL and Gadian, DG (1988) A 31P nuclear magnetic resonance study. in vivo of metabolic abnormalities in rats with acute liver failure NMR Biomedicine 1, 6773.Google ScholarPubMed
Brauer, M and Ling, M (1991) The effect of chronic ethanol consumption on the intact rat liver studied by in vivo 31P NMR spectroscopy. Magnetic Resonance in Medicine 20, 100112.CrossRefGoogle Scholar
Bowers, JL, Teramoto, K, Khettery, U and Clouse, ME (1992) Phosphorus-31 NMR assessment of orthotopic rat liver transplantation viability: the effect of warm ischemia .Transplantation 54, 604609.CrossRefGoogle Scholar
Chatterjee, R, Srinivasan, KS and Maiti, PC (1957) Cordyceps sinensis (Berkeley) Saccardo: structure of Cordycepic acid .Journal of the American Pharmaceutical Association 66, 114118.CrossRefGoogle Scholar
Desmoulin, F, Canioni, P, Crotte, C, Gérolami, A and Cozzone, PJ (1987) Hepatic metabolism during acute ethanol administration: a phosphorus-31 nuclear magnetic resonance study on the perfused rat liver under normoxic or hypoxic conditions. Hepatology 7, 315327.CrossRefGoogle ScholarPubMed
Furuya, T, Hirotani, M and Matsuzawa, M (1983) N6-(2-Hydroxyethyl) adenosine, a biologically active compound from cultured mycelia of Cordyceps and Isaria species. Phytochemistry 22, 25092512.CrossRefGoogle Scholar
Geoffrion, Y, Rydzy, M, Butler, KW, Smith, ICP and Jarrell, HC (1988) The use of immobilized ferrite to enhance the depth selectivity of in vivo surface coil NMR spectroscopy. NMR Biomedicine 1, 107112.CrossRefGoogle ScholarPubMed
Hamada, M (1991) Effect of Cordyceps sinensis (Berk.) Sacc. extract upon anti-tumor activity and tumor immunity. Journal of Kanazawa Medical University 16, 4654.Google Scholar
Hiyoshi, T, Akasu, F, Yoshisugu, M and Fujiwara, M (1996) Supplemental effects of Cordyceps sinensis extract on long distance runners. Japanese Journal of Physical Fitness and Sports Medicine 45, 205210.Google Scholar
Japanese Animal Care Committee (1981) –. Guide for the care and use of laboratory animals. Experimental Animal 30, 173178.Google Scholar
Malloy, CR, Cunningham, CC and Radda, GK (1986) The metabolic state of the rat liver in vivo measured by 31P-NMR spectroscopy. Biochimica et Biophysica Acta 885, 111.CrossRefGoogle ScholarPubMed
Manabe, N, Sugimoto, M, Azuma, Y, Taketomo, N, Yamashita, A, Tsuboi, H, Tsunoo, A, Kinjo, N, Nian-Lai, H and Miyamoto, H (1996) Effect of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism in the mouse. Japanese Journal of Pharmacology 70, 8588.CrossRefGoogle ScholarPubMed
Mattiello, J and Evelhoch, JL (1991) Relative volume-average murine tumor BF measurement via deuterium nuclear magnetic resonance spectroscopy .Magnetic Resonance in Medicine 18, 320334.CrossRefGoogle Scholar
Niwano, Y, Konaka, S, Uchida, M and Sugimoto, T (1986) Activation of mitochondrial functions by malotilate in relation to accelerated liver regeneration in partial hepatectomized rats .Japanese Journal of Pharmacology 42, 525529.CrossRefGoogle Scholar
Okunieff, P, Kallinowski, F, Vaupel, P and Neuringer, LJ (1988) Effects of hydralazine-induced vasodilation on the energy metabolism of murine tumors studied by in vivo 31-nuclear magnetic resonance spectroscopy. Journal of the National Cancer Institute 80, 745750.CrossRefGoogle Scholar
Sandhu, GS, Gonnella, NC, Kapeghian, JK, Plocinski, AK, Plutchok, J and Schlosser, MJ (1991) Evaluation of 31P NMR spectroscopy as an indicator of chemically induced hepatic toxicity in the rat: comparison with serum enzyme levels and pathology. NMR Biomedicine 4, 1215.CrossRefGoogle ScholarPubMed
Takahashi, H, Geoffrion, Y, Butler, KW and French, SW (1990) In vivo hepatic energy metabolism during the progression of alcoholic liver diseases: a noninvasive 31P nuclear magnetic resonance study in rats. Hepatology 11, 6573.CrossRefGoogle ScholarPubMed
Takahashi, K, Shigemori, S, Nosaka, S, Morikawa, S and Inubushi, T (1997) The effects of halothane and isoflurane on the phosphor-energetic state of the liver during hemorrhagic shock in rats: an in vivo 31P nuclear magnetic resonance spectroscopic study. Anesthesia and Analgesia 85, 347352.Google Scholar
Tozer, GM, Maxwell, RJ, Griffiths, JR and Pham, P (1990) Modification of the 31P magnetic resonance spectra of a rat tumour using vasodilators and its relationship to hypotension. British Journal of Cancer 62, 553560.CrossRefGoogle ScholarPubMed
Tsuhichita, H, Kobayashi, A, Kojima, T, Kuwata, T, Noguchi, T, Koto, K and Takahashi, T (1991) Bioavailability of iron from ferric pyrophosphate. Journal of Agricultural and Food Chemistry 39, 316321.CrossRefGoogle Scholar
Tsunoo Takemoto, AN & Kamijo, M (1995) Pharmacological effects of the mycelial extract of cultured Cordyceps sinensis on airways and aortae of the rat. In Science and Cultivation of Edible Fungi, pp. 425431 [Elliott, YJ, editor]. Rotterdam: Balkema.Google Scholar
Walsh, TR, Detre, JA, Koretsky, AP, Simplaceanu, E, Halow, JM, Rao, P, Makowka, L and Ho, C (1993) Response of normal and reperfused livers to glucagon stimulation: NMR detection of BF and high-energy phosphates. Biochimica et Biophysica Acta 1181, 714.CrossRefGoogle Scholar
Whalen, M and Shapiro, JI (1991) Controlled ventilation during NMR spectroscopic studies: hemodynamic and biochemical consequences. Magnetic Resonance Imaging 9, 229234.CrossRefGoogle ScholarPubMed
Wickramasinghe, SN (1988) Nutritional anaemia. Clinical and Laboratory Haematology 10, 117134.CrossRefGoogle Scholar
Yang, M, Shimada, H, Kobayashi, T, Niimoto, S and Nakagawara, G (1995) Predicting the viability of graft livers in rats through a rapid and sensitive metabolic indicator assessed by 31P-NMR spectroscopy. Surgery Today 25, 711716.CrossRefGoogle ScholarPubMed
Yoshida, J, Takamura, S, Suzuki, S and Koshimura, S (1992) Potentiating effect of an extract of Cordyceps sinensis (Berk.) Sacc. on cytostatic activity of mouse effector cells against tumor cells. Journal of Kanazawa Medical University 17, 330335.Google Scholar
Zhao, M, Fortan, LG and Evelhoch, JL (1995) The effects of isoflurane and halothane on blood flow and 31P NMR spectra in murine RIF-1 tumors. Magnetic Resonance in Medicine 33, 610618.CrossRefGoogle ScholarPubMed