Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-18T04:52:59.368Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of vitamin B6 deficiency and repletion on the uptake of steroid hormones into uterus slices and isolated liver cells of rats

Published online by Cambridge University Press:  09 March 2007

David A. Bender ,
Kweku Ghartey-Sam  and
Amerjit Singh
David A. Bender
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WCIE 6BT
Kweku Ghartey-Sam
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WCIE 6BT
Amerjit Singh
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WCIE 6BT
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. In vitro, pyridoxal phosphate extracts steroid-hormone receptors from tight nuclear binding (Cidlowski & Thanassi, 1981); in vitamin B6-deficient rats there is increased and prolonged nuclear accumulation of oestradiol in the uterus and testosterone in the prostate, associated with enhanced biological responsiveness of these target tissues to steroid hormone action (Symes et al. 1984; Bowden et al. 1986).

2. Slices of uterus from vitamin B6-deficient rats accumulated more [3H]oestradiol than did tissue from repleted animals. Acute repletion with vitamin B6 (0.5–1 h before killing) further increased the uptake of the steroid.

3. Isolated hepatocytes from vitamin B6-deficient rats accumulated more [3H]dexamethasone than did cells from repleted animals. Pre-incubation of the hepatocytes with pyridoxal phosphate resulted in a further increase in the uptake of the steroid.

4. The results suggest that in addition to the putative role of pyridoxal phosphate in releasing steroid-hormone-receptor complexes from tight nuclear binding (Cidlowski & Thanassi, 1981), vitamin B6 deficiency may also increase the concentration of steroid-hormone receptors or enzymes and other steroid-binding proteins in target tissues.

Type
Hormones, Metabolism
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 619 - 628
Copyright
Copyright © The Nutrition Society 1989

References

Adams, E. (1979) Fluorimetric determination of pyridoxal phosphate in enzymes. Methods in Enzymology 62, 407–410.CrossRefGoogle Scholar
Bayoumi, R. A. L., Kirwan, J. R. & Smith, W. R. D. (1972). Some effects of dietary vitamin B6 deficiency and 4-deoxypyridoxine on GABA metábolism in rat brain. Journal of Neurochemistry 19, 569–576.CrossRefGoogle Scholar
Bender, D. A., Bowden, J.-F., Coulson, W. F., Dewji, M. R., Sutton, J. & Symes, E. K. (1987) Vitamin B6 deficiency enhances end-organ sensitivity to steroid hormones. In Biochemistry of Vitamin B-6 pp. 395–406 [Korpela, T. and Cristen, P., editors]. Basel and Boston: Birkhauser Verlag.CrossRefGoogle Scholar
Bender, D. A. & Olufunwa, R. (1988) Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells. British Journal of Nutrition 59, 279–287.CrossRefGoogle ScholarPubMed
Bender, D. A., Tagoe, C. E. & Vale, J. A. (1982) Effects of oestrogen administration on vitamin B6 and tryptophan metabolism in the rat. British Journal of Nutrition 47, 609–614.CrossRefGoogle ScholarPubMed
Black, A. L., Guirard, B. M. & Snell, E. E. (1975) Increased muscle glycogen phosphorylase in rats fed high levels of vitamin B6. Journal of Nutrition 107, 1962–1968.CrossRefGoogle Scholar
Black, A. L., Guirard, B. M. & Snell, E. E. (1978) The behaviour of muscle glycogen phosphorylase as a reservoir for vitamin B6 in the rat. Journal of Nutrition 118, 670–677.CrossRefGoogle Scholar
Bowden, J.-F. J. (1985). Studies of the effects of vitamin B6 deficiency on the action of androgens and oestrogens in the rat. PhD Thesis, University of London.Google Scholar
Bowden, J.-F., Bender, D. A., Symes, E. K. & Coulson, W. F. (1986). Enhanced uterine uptake and nuclear retention of [3H]oestradiol through the oestrous cycle and enhanced end-organ sensitivity to oestrogen stimulation in vitamin B6 deficient rats. Journal of Steroid Biochemistry 25, 359–365.CrossRefGoogle Scholar
Burton, K. H. (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62, 315–323.CrossRefGoogle ScholarPubMed
Cake, M. H., DiSorbo, D. M. & Litwack, G. (1978) Effect of pyridoxal phosphate on the DNA binding site of activated glucocorticoid receptor. Journal of Biological Chemistry 253, 4886–4891.CrossRefGoogle Scholar
Chen, T. J., MacDonald, R. G., Robidoux, R. G. & Leavitt., W. W. (1981) Characterization and quantification of the PLP-extracted nuclear progesterone receptor. Journal of Steroid Biochemistry 14, 1023–1028.CrossRefGoogle ScholarPubMed
Cidlowski, J. A. & Thanassi, J. W. (1978) Extraction of nuclear glucorticoid-receptor complexes. Biochemical and Biophysical Research Communications 82, 1140–1146.CrossRefGoogle Scholar
Cidlowski, J. A. & Thanassi, J. W. (1981) Pyridoxal phosphate: a possible cofactor in steroid hormone action. Journal of Steroid Biochemistry 15, 11–16.CrossRefGoogle ScholarPubMed
Elliott, K. R. F., Ash, R., Pogson, C. I., Smith, S. A. & Crisp, D. M. (1976). In Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies pp. 139–143 [Tager, J.M., Soling, H. D. and Williamson, J. R., editors]. Amsterdam: North Holland.Google Scholar
Hiipakka, R. A. & Rao, S. (1980) Effect of pyridoxal phosphate on the androgen receptor from rat prostate: inhibition of receptor aggregation and receptor binding to nuclei and to DNA-cellulose. Journal of Steroid Biochemistry 13, 841–846.CrossRefGoogle ScholarPubMed
Holley, J., Bender, D. A., Coulson, W. F. & Symes, E. K. (1983). Effects of vitamin B6 nutritional status on the uptake of [3H]oestradiol into the uterus, liver and hypothalamus of the rat. Journal of Steroid Biochemistry 18, 161–165.CrossRefGoogle Scholar
Isomaa, V., Pajunen, A. E. I., Bardin, C. W. & Janne, O. A. (1982) Nuclear androgen receptors in the mouse kidney: validation of a new assay. Endrocrinology 111, 833–843.CrossRefGoogle ScholarPubMed
Lumeng, L., Brashear, R. E. & Li, T.-K. (1974) Pyridoxal phosphate in plasma: source, protein binding and cellular transport. Journal of Laboratory and Clinical Medicine 84, 334–343.Google ScholarPubMed
Majumder, P. K., Joshi, J. B. & Banerjee, M. R. (1983) Correlation between nuclear glucocorticoid receptor levels and casein gene expression in mouse mammary gland in vitro. Journal of Biological Chemistry 238, 6793–6798.CrossRefGoogle Scholar
Mulder, E., Vrij, L. & Foekins, J. A. (1980) Inhibition of the nucleic acid and chromatin binding of the rat prostate androgen receptor by pyridoxal phosphate, heparin and Cibacron blue. Steroids 36, 633–645.CrossRefGoogle ScholarPubMed
Muldoon, T. G. & Cidlowski, J. A. (1980) Specific modification of the rat uterine oestrogen receptor by pyridoxal phosphate. Journal of Biological Chemistry 255, 3100–3107.CrossRefGoogle Scholar
Muller, R. E., Traish, A. & Wotiz, H. H. (1980) Effects of pyridoxal 5'-phosphate on uterine oestrogen receptor (i) inhibition of nuclear binding in cell-free system and intact uterus. Journal of Biological Chemistry 255, 4062–4067.CrossRefGoogle ScholarPubMed
Nishigori, H., Moudgil, V. & Toft, D. (1978) Inactivation of avian receptor binding to ATP-sepharose by pyridoxal phosphate. Biochemical and Biophysical Research Communications 80, 112–118.CrossRefGoogle Scholar
Romero, F. J. & Viña, J. (1983) A simple procedure for the preparation of isolated liver cells. Biochemical Education 11, 135–137.CrossRefGoogle Scholar
Seeley, D. H., Mester, J., Baulieu, E.-E. & Wolfson, A. J. (1984) Characterization of the oestrogen receptor extracted from hen oviduct nuclei with pyridoxal phosphate. Endocrinology 114, 1740–1744.CrossRefGoogle ScholarPubMed
Symes, E. K., Bender, D. A., Bowden, J.-F. & Coulson, W. F. (1984). Increased target tissue uptake of, and sensitivity to, testosterone in the vitamin B6 deficient rat. Journal of Steroid Biochemistry 20, 1089–1093.CrossRefGoogle Scholar