Skip to main content
×
Home
    • Aa
    • Aa

Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet

  • Roman Thaler (a1), Heidrun Karlic (a2) (a3), Petra Rust (a1) and Alexander G. Haslberger (a1)
Abstract

The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen as the model system because epigenetic regulation has been previously shown in cell lines for this gene. Promoter methylation and gene expression of MnSOD in buccal swabs from three sample groups were analysed. The three groups included: (1) forty vegetarians (aged 20–30 years); (2) age-matched omnivores; (3) elderly omnivores (aged>85 years). A 3-fold increase in the expression of the MnSOD gene was associated with decreased CpG methylation of the analysed promoter region in the vegetarian group compared with the age-matched omnivores group. Expression and promoter methylation of the MnSOD gene in elderly omnivores showed no significant differences compared with younger omnivores. In accordance with previous findings in various tissues, DNA global methylation was found to be significantly higher (30 %) in buccal swabs of younger subjects (independent of the diet), than in those of elderly omnivores. In the control experiment which was designed to verify the findings of the human buccal swab studies, the Caco-2 cell line was treated with zebularine. Results of the control study showed a 6-fold increase of MnSOD expression, an approximately 40 % decreased methylation of specified CpG in the MnSOD promoter and a 50 % reduction of global DNA methylation. These results indicate that diet affects the epigenetic regulation of human MnSOD.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Epigenetic regulation of human buccal mucosa mitochondrial superoxide dismutase gene expression by diet
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Alexander G. Haslberger, fax +43 1 8795896, email alexander.haslberger@univie.ac.at
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1 FE Ahmed (2007) Colorectal cancer epigenetics: the role of environmental factors and the search for molecular biomarkers. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 25, 101154.

3 RA Liddle & RL Jirtle (2006) Epigenetic silencing of genes in human colon cancer. Gastroenterology 131, 960962.

4 DC Dolinoy , JR Weidman , RA Waterland & RL Jirtle (2006) Maternal genistein alters coat color and protects Avy mouse offspring from obesity by modifying the fetal epigenome. Environ Health Perspect 114, 567572.

5 A Haslberger , F Varga & H Karlic (2006) Recursive causality in evolution: a model for epigenetic mechanisms in cancer development. Med Hypotheses 67, 14481454.

6 RL Jirtle & MK Skinner (2007) Environmental epigenomics and disease susceptibility. Nat Rev Genet 8, 253262.

8 WY Tang & SM Ho (2007) Epigenetic reprogramming and imprinting in origins of disease. Rev Endocr Metab Disord 8, 173182.

9 JC Mathers (2006) Nutritional modulation of ageing: genomic and epigenetic approaches. Mech Ageing Dev 127, 584589.

12 PA Wade (2001) Methyl CpG binding proteins: coupling chromatin architecture to gene regulation. Oncogene 20, 31663173.

13 L Zhou , X Cheng , BA Connolly , MJ Dickman , PJ Hurd & DP Hornby (2002) Zebularine: a novel DNA methylation inhibitor that forms a covalent complex with DNA methyltransferases. J Mol Biol 321, 591599.

14 P Cheung & P Lau (2005) Epigenetic regulation by histone methylation and histone variants. Mol Endocrinol 19, 563573.

15 DC Baulcombe (2006) Short silencing RNA: the dark matter of genetics? Cold Spring Harb Symp Quant Biol 71, 1320.

16 G Kaati , LO Bygren & S Edvinsson (2002) Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period. Eur J Hum Genet 10, 682688.

17 G Kaati , LO Bygren , M Pembrey & M Sjostrom (2007) Transgenerational response to nutrition, early life circumstances and longevity. Eur J Hum Genet 15, 784790.

18 RA Waterland & RL Jirtle (2003) Transposable elements: targets for early nutritional effects on epigenetic gene regulation. Mol Cell Biol 23, 52935300.

19 J Geisel , H Schorr , M Bodis , S Isber , U Hübner , JP Knapp , R Obeid & W Herrmann (2005) The vegetarian lifestyle and DNA methylation. Clin Chem Lab Med 43, 11641169.

20 D Majchrzak , I Singer , M Männer , P Rust , D Genser , KH Wagner & I Elmadfa (2006) B-vitamin status and concentrations of homocysteine in Austrian omnivores, vegetarians and vegans. Ann Nutr Metab 50, 485491.

21 A Agrawal , RF Murphy & DK Agrawal (2007) DNA methylation in breast and colorectal cancers. Mod Pathol 20, 711721.

22 MF Fraga , R Agrelo & M Esteller (2007) Cross-talk between aging and cancer: the epigenetic language. Ann N Y Acad Sci 1100, 6074.

23 MF Fraga & M Esteller (2007) Epigenetics and aging: the targets and the marks. Trends Genet 23, 413418.

24 J Han , GA Colditz & DJ Hunter (2007) Manganese superoxide dismutase polymorphism and risk of skin cancer (United States). Cancer Causes Control 18, 7989.

25 J Marcinkiewicz , M Ciszek , M Bobek , M Strus , PB Heczko , M Kurnyta , R Biedroń & A Chmielarczyk (2007) Differential inflammatory mediator response in vitro from murine macrophages to lactobacilli and pathogenic intestinal bacteria. Int J Exp Pathol 88, 155164.

26 C Tang , BT Ang & S Pervaiz (2007) Cancer stem cell: target for anti-cancer therapy. FASEB J 21, 37773785.

28 DR Hodge , B Peng , C Pompeia , S Thomas , E Cho , PA Clausen , VE Marquez & WL Farrar (2005) Epigenetic silencing of manganese superoxide dismutase (SOD-2) in KAS 6/1 human multiple myeloma cells increases cell proliferation. Cancer Biol Ther 4, 585592.

29 PJ Hornsby (2007) Telomerase and the aging process. Exp Gerontol 42, 575581.

30 M Hitchins , R Williams , K Cheong , (2005) MLH1 germline epimutations as a factor in hereditary nonpolyposis colorectal cancer. Gastroenterology 129, 13921399.

31 DR Hodge , W Xiao , B Peng , JC Cherry , DJ Munroe & WL Farrar (2005) Enforced expression of superoxide dismutase 2/manganese superoxide dismutase disrupts autocrine interleukin-6 stimulation in human multiple myeloma cells and enhances dexamethasone-induced apoptosis. Cancer Res 65, 62556263.

32 Y Xu , S Porntadavity & DK St Clair (2002) Transcriptional regulation of the human manganese superoxide dismutase gene: the role of specificity protein 1 (Sp1) and activating protein-2 (AP-2). Biochem J 362, 401412.

34 MD Jamaluddin , I Chen , F Yang , X Jiang , M Jan , X Liu , AI Schafer , W Durante , X Yang & H Wang (2007) Homocysteine inhibits endothelial cell growth via DNA hypomethylation of the cyclin A gene. Blood 110, 36483655.

35 Y Jiang , T Sun , J Xiong , J Cao , G Li & S Wang (2007) Hyperhomocysteinemia-mediated DNA hypomethylation and its potential epigenetic role in rats. Acta Biochim Biophys Sin (Shanghai) 39, 657667.

36 NJ Mann , D Li , AJ Sinclair , NP Dudman , XW Guo , GR Elsworth , AK Wilson & FC Kelly (1999) The effect of diet on plasma homocysteine concentrations in healthy male subjects. Eur J Clin Nutr 53, 895899.

37 M Cravo , P Fidalgo , AD Pereira , A Gouveia-Oliveira , P Chaves , J Selhub , JB Mason , FC Mira & CN Leitao (1994) DNA methylation as an intermediate biomarker in colorectal cancer: modulation by folic acid supplementation. Eur J Cancer Prev 3, 473479.

39 YI Kim , HW Baik , K Fawaz , T Knox , YM Lee , R Norton , E Libby & JB Mason (2001) Effects of folate supplementation on two provisional molecular markers of colon cancer: a prospective, randomized trial. Am J Gastroenterol 96, 184195.

41 B Krawczyk & K Fabianowska-Majewska (2006) Alteration of DNA methylation status in K562 and MCF-7 cancer cell lines by nucleoside analogues. Nucleosides Nucleotides Nucleic Acids 25, 10291032.

43 MA Sanjoaquin , PN Appleby , M Thorogood , JI Mann & TJ Key (2004) Nutrition, lifestyle and colorectal cancer incidence: a prospective investigation of 10998 vegetarians and non-vegetarians in the United Kingdom. Br J Cancer 90, 118121.

44 M Thorogood , J Mann , P Appleby & K McPherson (1994) Risk of death from cancer and ischaemic heart disease in meat and non-meat eaters. Br Med J 308, 16671670.

46 S Haldar , IR Rowland , YA Barnett , I Bradbury , PJ Robson , J Powell & J Fletcher (2007) Influence of habitual diet on antioxidant status: a study in a population of vegetarians and omnivores. Eur J Clin Nutr 61, 10111022.

47 AL Rauma & H Mykkanen (2000) Antioxidant status in vegetarians versus omnivores. Nutrition 16, 111119.

48 PW Laird (2003) The power and the promise of DNA methylation markers. Nat Rev Cancer 3, 253266.

50 IB Van den Veyver (2002) Genetic effects of methylation diets. Annu Rev Nutr 22, 255282.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 7
Total number of PDF views: 89 *
Loading metrics...

Abstract views

Total abstract views: 139 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th May 2017. This data will be updated every 24 hours.