Skip to main content
×
Home
    • Aa
    • Aa

Evidence for an association between genetic variants of the fatty acid desaturase 1 fatty acid desaturase 2 (FADS1 FADS2) gene cluster and the fatty acid composition of erythrocyte membranes

  • Peter Rzehak (a1) (a2), Joachim Heinrich (a1), Norman Klopp (a1), Linda Schaeffer (a1), Sebastian Hoff (a3), Günther Wolfram (a4), Thomas Illig (a1) and Jakob Linseisen (a3) (a5)...
Abstract

The present study gives further evidence for the recently found association between variants of the fatty acid desaturase 1 fatty acid desaturase 2 (FADS1 FADS2) gene cluster and PUFA in blood phospholipids and explores this association for cellular fatty acids in erythrocyte membranes. In a subgroup of adults participating in the Bavarian Nutrition Survey II, a cross-sectional population-based study conducted in Bavaria, Germany, allelic variation in three selected loci of the FADS1 FADS2 gene cluster was analysed and used for haplotype construction. Associations with plasma phospholipid PUFA (n 163) and PUFA in erythrocyte membranes (n 535) were investigated by regression analysis. All haplotypes of the original five-loci haplotypes of our previous study could be replicated. In addition, associations with serum phospholipid PUFA were confirmed in the present data set. Although less pronounced, associations between FADS1 FADS2 haplotypes and PUFA in erythrocyte membranes, particularly arachidonic and dihomo-γ-linolenic acid, could be established. We provide the first replication of the association of the FADS1 FADS2 gene cluster with PUFA in blood phospholipids. For the first time, such associations were also shown for PUFA in cell membranes.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Evidence for an association between genetic variants of the fatty acid desaturase 1 fatty acid desaturase 2 (FADS1 FADS2) gene cluster and the fatty acid composition of erythrocyte membranes
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Evidence for an association between genetic variants of the fatty acid desaturase 1 fatty acid desaturase 2 (FADS1 FADS2) gene cluster and the fatty acid composition of erythrocyte membranes
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Evidence for an association between genetic variants of the fatty acid desaturase 1 fatty acid desaturase 2 (FADS1 FADS2) gene cluster and the fatty acid composition of erythrocyte membranes
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Peter Rzehak, fax +49 89 3187 3380, email peter.rzehak@helmholtz-muenchen.de
References
Hide All
1Schaeffer L, Gohlke H, Muller M, Heid IM, Palmer LJ, Kompauer I, Demmelmair H, Illig T, Koletzko B & Heinrich J (2006) Common genetic variants of the FADS1 FADS2 gene cluster and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids. Hum Mol Genet 15, 17451756.
2Cookson WO, Sharp PA, Faux JA & Hopkin JM (1989) Linkage between immunoglobulin E responses underlying asthma and rhinitis and chromosome 11q. Lancet i, 12921295.
3Cox HE, Moffatt MF, Faux JA, Walley AJ, Coleman R, Trembath RC, Cookson WO & Harper JI (1998) Association of atopic dermatitis to the β subunit of the high affinity immunoglobulin E receptor. Br J Dermatol 138, 182187.
4Huang JL, Gao PS, Mathias RA, et al. (2004) Sequence variants of the gene encoding chemoattractant receptor expressed on Th2 cells (CRTH2) are associated with asthma and differentially influence mRNA stability. Hum Mol Genet 13, 26912697.
5Stafford AN, Rider SH, Hopkin JM, Cookson WO & Monaco AP (1994) A 2.8 Mb YAC contig in 11q12-q13 localizes candidate genes for atopy: Fc epsilon RI β and CD20. Hum Mol Genet 3, 779785.
6Wheatley AP, Bolland DJ, Hewitt JE, Dewar JC & Hall IP (2002) Identification of the autoantigen SART-1 as a candidate gene for the development of atopy. Hum Mol Genet 11, 21432146.
7Hoff S, Seiler H, Heinrich J, et al. (2005) Allergic sensitisation and allergic rhinitis are associated with n-3 polyunsaturated fatty acids in the diet and in red blood cell membranes. Eur J Clin Nutr 59, 10711080.
8Golik A, Weissgarten J, Evans S, Cohen N, Averbukh Z, Zaidenstein R, Cotariu D & Modai D (1996) Erythrocyte Na+, K+ and Ca2+, Mg(2+)-ATPase activities in hypertensives on angiotensin-converting enzyme inhibitors. Clin Biochem 29, 249254.
9Folch J, Lees M & Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497509.
10Butte W (1983) Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification. J Chromatogr 261, 142145.
11Carter KW, McCaskie PA & Palmer LJ (2006) JLIN: a Java based linkage disequilibrium plotter. BMC Bioinformatics 7, 60.
12Schaid DJ, Rowland CM, Tines DE, Jacobson RM & Poland GA (2002) Score tests for association between traits and haplotypes when linkage phase is ambiguous. Am J Hum Genet 70, 425434.
13Nyholt DR (2004) A simple correction for multiple testing for single-nucleotide polymorphisms in linkage disequilibrium with each other. Am J Hum Genet 74, 765769.
14Arab L (2003) Biomarkers of fat and fatty acid intake. J Nutr 133, Suppl. 3, 925S932S.
15Kinsella JE, Lokesh B, Broughton S & Whelan J (1990) Dietary polyunsaturated fatty acids and eicosanoids: potential effects on the modulation of inflammatory and immune cells: an overview. Nutrition 6, 2444.
16Arterburn LM, Hall EB & Oken H (2006) Distribution, interconversion, and dose response of n-3 fatty acids in humans. Am J Clin Nutr 83, Suppl. 6, 1467S1476S.
17Burdge G (2004) α-Linolenic acid metabolism in men and women: nutritional and biological implications. Curr Opin Clin Nutr Metab Care 7, 137144.
18Cho HP, Nakamura MT & Clarke SD (1999) Cloning, expression, and nutritional regulation of the mammalian Δ-6 desaturase. J Biol Chem 274, 471477.
19Cho HP, Nakamura M & Clarke SD (1999) Cloning, expression, and fatty acid regulation of the human Δ-5 desaturase. J Biol Chem 274, 3733537339.
20Spielmann D, Bracco U, Traitler H, Crozier G, Holman R, Ward M & Cotter R (1988) Alternative lipids to usual omega 6 PUFAS: γ-linolenic acid, α-linolenic acid, stearidonic acid, EPA, etc. J Parenter Enteral Nutr 12, Suppl. 6, 111S123S.
21Rubin D & Laposata M (1992) Cellular interactions between n-6 and n-3 fatty acids: a mass analysis of fatty acid elongation/desaturation, distribution among complex lipids, and conversion to eicosanoids. J Lipid Res 33, 14311440.
22Calder PC (2005) Polyunsaturated fatty acids and inflammation. Biochem Soc Trans 33, 423427.
23Calder PC (2006) n-3 Polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr 83, Suppl. 6, 1505S1519S.
24Deckelbaum RJ, Worgall TS & Seo T (2006) n-3 Fatty acids and gene expression. Am J Clin Nutr 83, Suppl. 6, 1520S1525S.
25Serhan CN (2005) Novel eicosanoid and docosanoid mediators: resolvins, docosatrienes, and neuroprotectins. Curr Opin Clin Nutr Metab Care 8, 115121.
26Adam O, Wolfram G & Zollner N (2003) Influence of dietary linoleic acid intake with different fat intakes on arachidonic acid concentrations in plasma and platelet lipids and eicosanoid biosynthesis in female volunteers. Ann Nutr Metab 47, 3136.
27de Lorgeril M, Renaud S, Mamelle N, Salen P, Martin JL, Monjaud I, Guidollet J, Touboul P & Delaye J (1994) Mediterranean α-linolenic acid-rich diet in secondary prevention of coronary heart disease. Lancet 343, 14541459.
28Adam O, Beringer C, Kless T, Lemmen C, Adam A, Wiseman M, Adam P, Klimmek R & Forth W (2003) Anti-inflammatory effects of a low arachidonic acid diet and fish oil in patients with rheumatoid arthritis. Rheumatol Int 23, 2736.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 6
Total number of PDF views: 185 *
Loading metrics...

Abstract views

Total abstract views: 272 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 19th October 2017. This data will be updated every 24 hours.