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Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer

  • B. Pool-Zobel (a1), J. van Loo (a2), I. Rowland (a3) and M. B. Roberfroid (a4)
Abstract

Inulin is extracted from the chicory root. It is a set of fructans with its monomers (n=2–65) linked by means of β(2–1) bonds. This linkage cannot be hydrolysed by either pancreatic or by brush border digestive enzymes in the upper intestinal tract of humans. As such the carbohydrates arrive in the colon, where they are fermented by bifidobacteria and other lactic acid producing bacteria, thus enhancing their relative populations in the gut. Recent research in experimental animal models revealed that inulin has significant anticarcinogenic properties. It acts chemopreventively by reducing the incidence of azoxymethane (AOM) — induced aberrant crypt foci and tumours in the colon. These effects may be due to the stimulation of bifidobacteria, which themselves have been shown to act as antigenotoxic in the colon and to reduce AOM-induced tumours. Also fermentation products, including the short-chain fatty acid butyrate, could contribute to the protective effects. In this case a mechanism may be the induction of apoptosis of already transformed cells. The experimental evidence from animal studies and from studies elucidating potential mechanisms strongly supports the possibility that inulin will contribute to reducing risks for colon cancer in humans. In order to obtain more insight into this possibility, human dietary intervention studies relating biomarkers of reduced risk to inulin consumption are needed.

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Corresponding author
*Corresponding author: Prof. Dr. rer. nat. Beatrice L. Pool-Zobel, tel +49 3641 949670, fax +49 3641 949672, email b8pobe@uni-jena.de
References
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Abrahamse, SL, Pool-Zobel, BL & Rechkemmer, G (1999) Potential of short chain fatty acids to modulate the induction of DNA damage and changes in the intracellular calcium concentration in isolated rat colon cells. Carcinogenesis 20, 629634.
Acheson, ED, Hadfield, EH & Macbeth, RG (1967) Carcinoma of the nasal cavity and accessory sinuses in woodworkers. The Lancet 2/11, 311312.
Awasthi, YC, Sharma, R & Singhal, SS (1994) Human glutathione S-transferases. International Journal of Biochemistry 26, 295308.
Ballongue, J, Schumann, C & Quignon, P (1997) Effects of lactulose and lactitol on colonic microflora and enzymatic activity. Scandinavian Journal of Gastroenterology 32, Suppl. 222, 4144.
Bird, RP (1987) Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings. Cancer Letters 37, 147151.
Bolognani, F, Rumney, CJ, Coutts, JT, Pool-Zobel, BL & Rowland, IR (2001) Effect of lactobacilli, bifidobacteria and inulin on the formation of aberrant crypt foci in rats. European Journal of Nutrition 40, 293300.
Cohen, JJ (1993) Apoptosis. Immunology Today 14, 126130.
Coles, B, Yang, M, Lang, NP & Kadlubar, FF (2000) Expression of hGSTP1 alleles in human lung and catalytic activity of the native protein variants towards 1-chloro-2,4-dinitrobenzene, 4-vinylpridine and (+)-anitbenzo[a]pyrene-7,8-diol-9,10-0x9de. Cancer Letters 156, 167175.
Coudray, C, Bellanger, J, Castiglia-Delavaud, C, Remesy, C, Vermorel, M & Rayssiguier, Y (1997) Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. European Journal of Clinical Nutrition 51, 375380.
Cummings, JH, Pomare, EW, Branch, WJ, Naylor, CPE & Macfarlane, GT (1987) Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 28, 12211227.
Den Hond, EM, Geypens, B & Ghoos, Y (2000) Effect of high performance chicory inulin on constipation. Nutrition Research 20, 731736.
Doll, R (1991) The lessons of life: Keynote address to the Nutrition and Cancer Conference. Cancer Research 52, Supplement, 2024S2029S.
Doll, R (1996) Nature and nurture: possibilities for cancer control. Carcinogenesis 17, 177184.
Doll, R & Peto, R (1981) The causes of cancer: Quantitative estimates of avoidable risks of cancer in the United States today. Journal of the National Cancer Institute 66, 11911308.
Ellgard, L, Andersson, H, Bosaeus, I & Bosaeus, I (1997) Inulin and oligofructose do not influence the absorption of cholestrol, or the excretion of cholesterol, Ca, Mg, Zn, Fe, or bile acids but increase energy excretion in ileostomy subjects. European Journal of Clinical Nutrition 45, 451457.
Fearon, ER (1997) Human cancer syndromes: Clues to the origin and nature of cancer. Science 278, 10431050.
Gallaher, DD & Khil, J (1999) The effect of synbiotics on colon carcinogenesis in rats. Journal of Nutrition 129, 1483S1487S.
Gibson, GR, Beatty, ER, Wang, X & Cummings, J (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975982.
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. Journal of Nutrition 125, 14011412.
Gibson, GR & Wang, X (1994) Regulatory effects of bifidobacteria on the growth of other colonic bacteria. Journal of Applied Bacteriology 77, 412420.
Hague, A, Elder, DJE, Hicks, DJ & Pareskeva, C (1995) Apoptosis in colorectal tumour cells: Induction by the short chain fatty acids butyrate, propionate and acetate and by the bile salt deoxycholate. International Journal of Cancer 60, 400406.
Hague, A & Paraskeva, C (1995) The short-chain fatty acid butyrate induces apoptosis in colorectal tumour cell lines. European Journal of Cancer Prevention 4, 359364.
Hale, AJ, Smith, CA, Sutherland, LC, Stoneman, VEA, Longthorne, VL, Culhane, AC & Williams, GT (1996) Apoptosis: molecular regulation of cell death. European Journal of Biochemistry 236, 126.
Hollstein, M, Sidransky, D, Vogelstein, B & Harris, CC (1991) P53 mutations in human cancers. Science 253, 4953.
Hughes, R & Rowland, IR (2001) Stimulation of apoptosis by two prebiotic chicory fructans in the rat colon. Carcinogenesis 22, 4347.
Ishikawa, T (1992) The ATP-dependent glutathione S-conjugate export pump. TIBS 17, 463468.
Johnson, IT, Williamson, G & Musk, SRR (1994) Anticarcinogenic factors in plant foods: A new class of nutrients? Nutrition Research Reviews 7, 175204.
Kassie, F, Pool-Zobel, BL, Parzefall, W, Schulte-Hermann, R & Knasmuller, S (1999) Investigations on the genotoxic effects of benzylisothiocyanate, a natural chemopreventive agent. Mutagenesis 14, 595603.
Kerr, JFR, Wyllie, AH & Currie, AR (1972) Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kinetics. British Journal of Cancer 26, 239257.
Kirlin, WG, Cai, J, Delong, MJ, Patten, EJ & Jones, DP (1999) Dietary compounds that induce cancer preventive phase 2 enzymes activate apoptosis at comparable doses in HT29 colon carcinoma cells. Journal of Nutrition 129, 18271835.
Kleessen, B, Sykura, B, Zunft, HJ & Blaut, M (1997) Effects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. American Journal of Clinical Nutrition 65, 13971402.
Kroemer, G, Petit, P, Zamzami, N, Vayssiere, JL & Mignotte, B (1995) The biochemistry of programmed cell death. FASEB Journal 9, 12771287.
Kruse, HP, Kleessen, B & Blaut, M (1999) Effects of inulin on faecal bifidobacteria in human subjects. British Journal of Nutrition 82, 375382.
Ling, WH, Korpela, R, Mykkanen, H, Salminen, S & Hanninen, O (1994) Lactobacillus strain Gg supplementation decreases colonic hydrolytic and reductive enzyme activities in healthy female adults. Journal of Nutrition 124, 1823.
Lupton, JR (1995) Butyrate and colonic cytokinetics: differences between in vitro and in vivo studies. European Journal of Cancer Prevention 4, 373378.
Magnuson, B, Carr, I & Bird, RP (1993) Ability of aberrant crypt foci characteristics to predict colonic tumor incidence in rats fed cholic acid. Cancer Research 53, 44994504.
McIntyre, A, Gibson, PR & Young, GP (1993) Butyrate production from dietary fibre and protection against large bowel cancer in a rat model. Gut 34, 386391.
Morra, G & Boland, CR (1995) Heriditary nonpolyposis colorectal cancer: the syndrome, the genes and historical perspectives. Journal of the National Cancer Institute 87, 11141125.
Moshfegh, AJ, Friday, JE, Goldman, JP & Chug-Ahuja, JK (1999) Presence of inulin and oligofructose in the diets of Americans. British Journal of Nutrition 129, 1407S1411S.
Mutanen, M, Pajari, AM & Oikarinen, SI (2000) Beef induces and rye bran prevents the formation of intestinal polyps in ApcMin mice: relation to β-catenin and PKC isozymes. Carcinogenesis 21, 11671173.
Nijhoff, WA, Mulder, TPJ, Verhagen, H, Van Poppel, G & Peters, WHM (1995) Effects of consumption of Brussels sprouts on plasma and urinary glutathione S-transferase class-α and -π in humans. Carcinogenesis 16, 955957.
Osswald, K, Becker, TW, Grimm, M, Jahreis, G & Pool-Zobel, BL (2000) Inter-and Intra-individual variation of faecal water – genotoxicity in human colon cells. Mutation Reserch 472, 5970.
Pereira, MA, Barnes, LH, Rassman, VL, Kelloff, GV & Steele, VE (1994) Use of azoxymethane-induced foci of aberrant crypts in rat colon to identify potential cancer chemopreventive agents. Carcinogenesis 15, 10491054.
Perrin, P, Pierre, F, Patry, Y, Champ, M, Berreur, M, Pradal, G, Bornet, P, Meflah, K & Menenteau, J (2001) Only fibres promoting a stable butyrate producing colonic ecosystem decrease the rate of aberrant crypt foci in rats. Gut 48, 5361.
Peters, WHM, Roelofs, HMJ, Nagengast, FM & Van Tongeren, JHM (1989) Human intestinal glutathione S-transferases. Biochem Journal 257, 471476.
Pierre, F, Perrin, P, Champ, M, Bornet, F, Meflah, K & Menanteau, J (1997) Short chain fructo-oligosaccharides reduce the occurrence of colon tumors and develop gut associated lymphoid tissue in Min mice. Cancer Research 57, 225228.
Pool-Zobel, BL, Abrahamse, SL, Collins, AR, Kark, W, Gugler, R, Oberreuther, D, Siegel, EG, Tretow-Van Lishaut, S & Rechkemmer, G (1999) Analysis of DNA strand breaks, oxidized bases and glutathione S-transferase P1 in human colon cells. Cancer Epidemiology Biomedical Preview 8, 609614.
Pool-Zobel, BL, Abrahamse, SL & Rechkemmer, G (1995) Pretreatment of colon cells with sodium butyrate but not iso-butyrate protects them from DNA damage induced by hydrogen peroxide. Proceedings, (Abstract).
Pool-Zobel, BL & Leucht, U (1997) Induction of DNA damage in human colon cells derived from biopsies by suggested risk factors of colon cancer. Mutation Research 375, 105116.
Pool-Zobel, BL, Neudecker, C, Domizlaff, I, Ji, S, Schillinger, U, Rumney, CJ, Moretti, M, Villarini, M, Scassellati-Sforzolini, G & Rowland, IR (1996) Lactobacillus-and Bifidobacterium-mediated antigenotoxicity in colon cells of rats: Prevention of carcinogen-induced damage in vivo and elucidation of involved mechanisms. Nutrition Cancer 26, 365380.
Reddy, BS (1998) Prevention of colon cancer by pre and probiotics: evidence from laboratory studies. British Journal of Nutrition 80, S219S223.
Reddy, BS, Hamid, R & Rao, CV (1997) Effect of dietary oligofructose and inulin on colonic preneoplastic aberrant crypt foci inhibition. Carcinogenesis 18, 13711374.
Rehman, A, Collis, CS, Yang, M, Kelly, M, Diplock, AT, Halliwell, B & Rice-Evans, CA (1998) The effects of iron and vitamin C co-supplementation on oxidative dmage to DNA in healthy volunteers. Biochemistry Biophysiology Research Commun 246, 293298.
Roberfroid, MB, Van Loo, J & Gibson, GR (1998) The Bifidogenic nature of chicory inulin and its hydrolysis products. Journal of Nutrition 128, 1119.
Roediger, WEW (1989) The utilisation of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 83, 424429.
Roland, N, Migpm-Baudon, L, Flinois, JP & Beaune, PH (1994) Hepatic and Intestinal cytochrome P450, Glutathone s-transferase and UDP Glucuronosyl transferase are affected by six types of dietary fiber in rats inoculated with human whole fecal flora. Journal of Nutrition 124, 15811587.
Roland, N, Rabot, B & Nugon-Baudon, L (1996) Modulation of the biological effects of glucosinolates by inulin and oat fibre in gnotobiotic rats inoculated with a human whole faecal flora. Food and Chemical Toxicology 34, 671677.
Rowland, IR (1991) Nutrition and gut flora metabolism. In Nutrition, Toxicity and Cancer, pp. 113136 [Rowland, IR, editor]. Boca Raton, FL: CRC Press.
Rowland, IR, Rumney, CJ, Coutts, JT & Lievense, LC (1998) Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis 19, 281285.
Saavedra, JM, Tscherina, A, Moore, N, Abi-Hanna, A, Coletta, F, Emenhiser, C & Yolken, RH (1999) Gastro-ntestinal function in infants consumin a weaning food supplemented with oligo-fructose, a prebiotic. Journal of Paediatrics, Gastroenterology and Nutrition 29, A95.
Schneemann, BO (1999) Fiber, inulin and oligofructose: similarities and differences. British Journal of Nutrition 129, 1424S1427S.
Sesink, ALA, Termont, DSML, Kleibeuker, JH & Van der Meer, R (2000) Red meat and colon cancer: dietary heme, but not fat, has cytotoxic and hyperproliferative effects on rat colonic epithelium. Carcinogenesis 21, 19091915.
Singh, J, Rivenson, A, Tomita, M, Shimamura, S, Ishibashi, N & Reddy, BS (1997) Bifidobacterium longum, a lactic acid-producing intestinal microflora inhibit colon cancer and modulate the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 18, 13711377.
Stein, J, Schroder, O, Bonk, M, Oremek, G, Lorenz, M & Caspary, WF (1996) Induction of glutathione-S-transferase-pi by short-chain fatty acids in the intestinal cell line Caco-2. European Journal of Clinical Investigation 26, 8487.
Steller, H (1995) Mechanisms and genes of cellular suicide. Science 267, 14451449.
Thompson, CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267, 14561462.
Treptow-van Lishaut, S, Rechkemmer, G, Rowland, IR, Dolara, P & Pool-Zobel, BL (1999) The carbohydrate crystalean and colonic microflora modulate expression of glutathione S-transferase subunits in colon of rats. European Journal of Nutrition 38, 7683.
Turesky, RJ, Lang, NP, Butler, MA, Teitel, CH & Kadlubar, FF (1991) Metabolic activation of carcinogenic heterocyclic aromatic amines by human liver and colon. Carcinogenesis 12, 18391845.
Van den Heufel, E, Muys, T, Van Dokkum, W & Schaafsma, G (1999) Oligofructose stimulates calcium absorption in adolescents. American Journal of Clinical Nutrition 35, 525552.
Van Lieshout, EMM, Peters, WHM & Jansen, JB (1996) Effect of oltipraz, alpha-tocopherol, betacarotene and phenethylisothiocyanate on rat oesopageal, gastric, colonic and heptatic glutathone, glutathione S-transferase and peroxidase. Carcinogenesis 17, 14391445.
Van Loo, J, Coussement, P, De Leenheer, L, Hoebregs, H & Smits, G (1995) On the presence of inulin and oligofructose as natural ingredients in the Western diet. Crititcal Reviews of Food Science Nutrition 35, 525552.
Van Loo, J, Cummings, JH, Delzenne, N, Englyst, HN, Franck, A, Hopkins, MJ, Kok, N, Macfarlane, GT, Newton, DF, Quigley, ME, Roberfroid, MR, van Vliet, T & Van den Heuvel, EGH (1999) Functional food properties of non digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095). British Journal of Nutrition 81, 121132.
Van Loo, J & Jonkers, N (2001) Evaluation in human volunteers of the potential anticarcinogenic activities of novel nutritional concepts prebiotics, probiotics and synbiotics (the SYNCAN project QLK1-1999-00346). Nutrition Metabolism Cardiovascular Disease 11, Suppl to No 4, 8793.
Van Munster, IP, Tangerman, A & Nagengast, FM (1994) Effect of resistant starch on colonic fermentation, bile acid metabolism, and mucosal proliferation. Digestive Diseases and Sciences 39, 834842.
Verghese, M, Rao, DR, Chawan, CB & Schackelford, LA (2002 a) Dietary inulin suppresses azoxymethane-induced preneoplastic aberrant crypt foci in mature Fisher-344 rats. Journal of Nutrition (in press).
Verghese, M, Rao, DR, Williams, LL & Schackelford, LA (2002 b) Dietary inulin suppresses azoxymethane-induced aberrant crypt foci and colon tumors at the promotion stage in Fisher-344 rats. Journal of Nutrition (in press).
Wargovich, MJ, Harris, C, Chen, CD, Palmer, C, Steele, VE & Kelloff, G (1992) Growth kinetics and chemoprevention of aberrant crypts in the rat colon. Journal of Cellular Biochemistry 15G, Supplement, 5154.
Wattenberg, LW (1992) Inhibition of carcinogenesis by minor dietary constituents. Cancer Research 52, Suppl., 2085S2091S.
Williams, CM (1999) Effects of inulin on lipid parameters in humans. British Journal of Nutrition 129, 1471S1473S.
Wollowski, I, Ji, S, Bakalinsky, AT, Neudecker, C & Pool-Zobel, BL (1999) Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats. Journal of Nutrition 129, 7782.
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