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Fish intake, erythrocyte n-3 fatty acid status and metabolic health in Danish adolescent girls and boys

  • Lotte Lauritzen (a1), Laurine B. S. Harsløf (a1), Lars I. Hellgren (a2), Maiken H. Pedersen (a1) (a2), Christian Mølgaard (a1) and Kim F. Michaelsen (a1)...
Abstract

Marine n-3 long-chain PUFA (n-3 LCPUFA) may have a beneficial effect on several aspects of the metabolic syndrome (dyslipidaemia, insulin resistance, hypertension and abdominal obesity). The metabolic syndrome is increasing in prevalence during adolescence, but only few studies have investigated the effects of n-3 LCPUFA in adolescence. The present study examines associations between fish intake (assessed by a 7 d pre-coded food diary), erythrocyte (RBC) DHA status (analysed by GC) and metabolic syndrome measures (anthropometry, blood pressure and plasma lipids, insulin and glucose) in 109 17-year-old children from the Copenhagen Birth Cohort Study. Of the children, 8 % were overweight or obese and few showed signs of the metabolic syndrome, but all the metabolic syndrome variables were correlated. Median fish intake was 10·7 (interquartile range 3·6–21·2) g/d. Boys tended to have a higher fish intake (P = 0·052), but girls had significantly higher RBC levels of DHA (P = 0·001). Sex and fish intake explained 37 % of the variance in RBC-DHA (P < 0·001). After adjusting for confounders, high DHA status was found to be significantly correlated with higher systolic blood pressure (P = 0·014) and increased fasting insulin (P = 0·018), but no adverse association was observed with the mean metabolic syndrome z-score. Overall, the present study showed the expected association between fish intake and RBC-DHA, which in contrast to our expectations tended to be associated with a poorer metabolic profile. Whether these results reflect the physiological function of n-3 LCPUFA, lifestyle factors associated with fish intake in Denmark, or mere chance remains to be investigated.

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Corresponding author
*Corresponding author: Dr Lotte Lauritzen, fax +45 3533 2483, email ll@life.ku.dk
References
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1 Geleijnse JM, Giltay EJ, Grobbee DE, et al. (2002) Blood pressure response to fish oil supplementation: metaregression analysis of randomized trials. J Hypertens 20, 14931499.
2 Hooper L, Thompson RL & Harrison RA, et al. (2004) Omega 3 fatty acids for prevention and treatment of cardiovascular disease. Cochrane Database of Systematic Reviews, Issue 4, CD003177. http://www.mrw.interscience.wiley.com/cochrane/clsysrev/articles/CD003177/frame.htlm.
3 Ghafoorunissa , Ibrahim A, Rajkumar L, et al. (2005) Dietary (n-3) long-chain polyunsaturated fatty acids prevent sucrose-induced insulin resistance in rats. J Nutr 135, 26342638.
4 Storlien LH, Kraegen EW, Chisholm DJ, et al. (1987) Fish oil prevents insulin resistance induced by high-fat feeding in rats. Science 237, 885888.
5 Haugaard SB, Madsbad S, Høy CE, et al. (2006) Dietary intervention increases n-3 long-chain polyunsaturated fatty acids in skeletal muscle membrane phospholipids of obese subjects. Implications for insulin sensitivity. Clin Endocrinol 64, 169178.
6 Clore JN, Harris PA, Li J, et al. (2000) Changes in phosphatidylcholine fatty acid composition are associated with altered skeletal muscle insulin responsiveness in normal man. Metab Clin Exp 49, 232238.
7 Delarue J, Li CH, Cohen R, et al. (2006) Interaction of fish oil and a glucocorticoid on metabolic responses to an oral glucose load in healthy human subjects. Br J Nutr 95, 267272.
8 Spadaro L, Magliocco O, Spampinato D, et al. (2008) Effects of n-3 polyunsaturated fatty acids in subjects with nonalcoholic fatty liver disease. Digest Liver Dis 40, 194199.
9 Ramel A, Martinez A, Kiely M, et al. (2008) Beneficial effects of long-chain n-3 fatty acids included in an energy-restricted diet on insulin resistance in overweight and obese European young adults. Diabetologia 51, 12611268.
10 Browning LM, Krebs JD, Moore CS, et al. (2007) The impact of long-chain n-3 polyunsaturated fatty acid supplementation on inflammation, insulin sensitivity and CVD risk in a group of overweight women with an inflammatory phenotype. Diabet Obes Metab 9, 7080.
11 Riserus U, Willett WC & Hu FB (2009) Dietary fats and prevention of type 2 diabetes. Prog Lipid Res 48, 4451.
12 Hartweg J, Farmer AJ, Holman RR, et al. (2009) Potential impact of omega-3 treatment on cardiovascular disease in type 2 diabetes. Curr Opin Lipidol 20, 3038.
13 Lauritzen L, Hansen HS, Jørgensen MH, et al. (2001) The essentiality of long-chain n-3 fatty acids in relation to development and function of the brain and retina. Prog Lipid Res 40, 194.
14 Damsgaard CT, Schack-Nielsen L, Michaelsen KF, et al. (2006) Fish oil affects blood pressure and the plasma lipid profile in healthy Danish infants. J Nutr 136, 9499.
15 Michaelsen KF (1997) Nutrition and growth during infancy. The Copenhagen Cohort Study. Acta Paediatr Suppl 420, 136.
16 Budek AZ, Hoppe C, Ingstrup H, et al. (2007) Dietary protein intake and bone mineral content in adolescents – The Copenhagen Cohort Study. Osteoporos Int 18, 16611667.
17 Mølgaard C, Thomsen BL & Michaelsen KF (2001) The influence of calcium intake and physical activity on bone mineral content and bone size in healthy children and adolescents. Osteoporos Int 12, 887894.
18 Lauritzen L, Jørgensen MH, Mikkelsen TB, et al. (2004) Maternal fish oil supplementation in lactation: effect on visual acuity and n-3 fatty acid content of infant erythrocytes. Lipids 39, 195206.
19 Friedewald WT, Levy RI & Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18, 499502.
20 Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (2001) Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA 285, 24862497.
21 Olsen SF, Hansen HS, Sandström B, et al. (1995) Erythrocyte levels compared with reported dietary intake of marine n-3 fatty acids in pregnant women. Br J Nutr 73, 387395.
22 Hibbeln JR, Nieminen LR, Blasbalg TL, et al. (2006) Healthy intakes of n-3 and n-6 fatty acids: estimations considering worldwide diversity. Am J Clin Nutr 83, S1483S1493.
23 Damsgaard CT, Frøkiær H & Lauritzen L (2008) The effects of fish oil and high or low linoleic acid intake on fatty acid composition of human peripheral blood mononuclear cells. Br J Nutr 99, 147154.
24 Hoyos C, Almqvist C, Garden F, et al. (2008) Effect of omega 3 and omega 6 fatty acid intakes from diet and supplements on plasma fatty acid levels in the first 3 years of life. Asia Pacific J Clin Nutr 17, 552557.
25 Warensjö E, Sundström J, Lind L, et al. (2006) Factor analysis of fatty acids in serum lipids as a measure of dietary fat quality in relation to the metabolic syndrome in men. Am J Clin Nutr 84, 442448.
26 Asserhøj M, Nehammer S, Matthiessen J, et al. (2009) Maternal fish oil supplementation during lactation may adversely affect long-term blood pressure, energy intake, and physical activity of 7-year-old boys. J Nutr 139, 298304.
27 Armitage JA, Pearce AD, Sinclair AJ, et al. (2003) Increased blood pressure later in life may be associated with perinatal n-3 fatty acid deficiency. Lipids 38, 459464.
28 Forsyth JS, Willatts P, Agostoni C, et al. (2003) Long-chain polyunsaturated fatty acid supplementation in infant formula and blood pressure in later childhood: follow up of a randomised controlled trial. BMJ 326, 953957.
29 Lee DH, Lee IK, Porta M, et al. (2007) Relationship between serum concentrations of persistent organic pollutants and the prevalence of metabolic syndrome among non-diabetic adults: results from the National Health and Nutrition Examination Survey 1999–2002. Diabetologia 50, 18411851.
30 Mozaffarian D (2009) Fish, mercury, selenium and cardiovascular risk: current evidence and unanswered questions. Int J Environ Res Public Health 6, 18941916.
31 Madsen L, Petersen RK & Kristiansen K (2005) Regulation of adipocyte differentiation and function by polyunsaturated fatty acids. Biochim Biophys Acta-Mol Basis Dis 1740, 266286.
32 Madsen L, Pedersen LM, Liaset B, et al. (2008) cAMP-dependent signaling regulates the adipogenic effect of n-6 polyunsaturated fatty acids. J Biol Chem 283, 71967205.
33 Crowe FL, Skeaff CM, Green TJ, et al. (2008) Serum n-3 long-chain PUFA differ by sex and age in a population-based survey of New Zealand adolescents and adults. Br J Nutr 99, 168174.
34 Bakewell L, Burdge GC & Calder PC (2006) Polyunsaturated fatty acid concentrations in young men and women consuming their habitual diets. Br J Nutr 96, 9399.
35 Giltay EJ, Gooren LJ, Toorians AW, et al. (2004) Docosahexaenoic acid concentrations are higher in women than in men because of estrogenic effects. Am J Clin Nutr 80, 11671174.
36 Burdge GC & Wootton SA (2002) Conversion of α-linolenic acid to eicosapentaenoic, docosapentaenoic and docosahexaenoic acids in young women. Br J Nutr 88, 411420.
37 Gerster H (1998) Can adults adequately convert α-linolenic acid (18:3n-3) to eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3)? Int J Vitam Nutr Res 68, 159173.
38 Burdge GC & Calder PC (2005) α-Linolenic acid metabolism in adult humans: the effects of gender and age on conversion to longer-chain polyunsaturated fatty acids. Eur J Lipid Sci Technol 107, 426439.
39 Burdge GC, Slater-Jefferies JL, Grant RA, et al. (2008) Sex, but not maternal protein or folic acid intake, determines the fatty acid composition of hepatic phospholipids, but not of triacylglycerol, in adult rats. Prostaglandins Leukot Essent Fatty Acids 78, 7379.
40 Schaeffer L, Gohlke H, Muller M, et al. (2006) Common genetic variants of the FADS1 FADS2 gene cluster and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids. Hum Mol Genet 15, 17451756.
41 Malerba G, Schaeffer L, Xumerle L, et al. (2008) SNPs of the FADS gene cluster are associated with polyunsaturated fatty acids in a cohort of patients with cardiovascular disease. Lipids 43, 289299.
42 Giltay EJ, Duschek EJ, Katan MB, et al. (2004) Raloxifene and hormone replacement therapy increase arachidonic acid and docosahexaenoic acid levels in postmenopausal women. J Endocrinol 182, 399408.
43 Caspi A, Williams B, Kim-Cohen J, et al. (2007) Moderation of breastfeeding effects on the IQ by genetic variation in fatty acid metabolism. Proc Natl Acad Sci U S A 104, 1886018865.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
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