Skip to main content
×
Home
    • Aa
    • Aa

Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice

  • S. Fukumitsu (a1), K. Aida (a1), N. Ueno (a1), S. Ozawa (a2), Y. Takahashi (a3) and M. Kobori (a3)...
Abstract

Flaxseed lignan secoisolariciresinol diglucoside (SDG) has been reported to prevent and alleviate lifestyle-related diseases including diabetes and hypercholesterolaemic atherosclerosis. This study assesses the effect of SDG on the development of diet-induced obesity in mice and the effect of the SDG metabolite enterodiol (END) on adipogenesis in 3T3-L1 adipocytes. We compared body weight, visceral fat weight, liver fat content, serum parameters, mRNA levels of lipid metabolism-related enzymes and adiponectin in mice fed either a low-fat diet (5 % TAG), high-fat diet (30 % TAG) or high-fat diet containing 0·5 and 1·0 % (w/w) SDG for 4 weeks. Administration of SDG to mice significantly reduced high-fat diet-induced visceral and liver fat accumulation, hyperlipaemia, hypercholesterolaemia, hyperinsulinaemia and hyperleptinaemia. SDG also suppressed sterol regulatory element binding protein 1c mRNA level in the liver and induced increases in the adiponectin mRNA level in the white adipose tissue and carnitine palmitoyltransferase I mRNA level in the skeletal muscle. Differentiated 3T3-L1 adipocytes were treated with 0, 5, 10 and 20 μmol/l END and then assayed for mRNA expression of adipogenesis-related genes and DNA binding activity of PPARγ to the PPAR response element consensus sequence. END induced adipogenesis-related gene mRNA expression including adiponectin, leptin, glucose transporter 4 and PPARγ, and induced PPARγ DNA binding activity in 3T3-L1 adipocytes. In conclusion, SDG induced adiponectin mRNA expression and showed beneficial effects on lipid metabolism in diet-induced obesity in mice. Flaxseed lignans are suggested to regulate adipogenesis-related gene expressions through an increase in PPARγ DNA binding activity.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Masuko Kobori, fax +81 29 838 7996, email kobori@affrc.go.jp
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1 MJ Pagliassotti , EC Gayles & JO Hill (1997) Fat and energy balance. Ann N Y Acad Sci 827, 431448.

2 J Triscari , C Nauss-Karol , BE Levin & AC Sullivan (1985) Changes in lipid metabolism in diet-induced obesity. Metabolism 34, 580587.

4 LU Thompson , SE Rickard , F Cheung , EO Kenaschuk & WR Obermeyer (1997) Variability in anticancer lignan levels in flaxseed. Nutr Cancer 27, 2630.

5 SC Cunnane , S Ganguli , C Menard , (1993) High alpha-linolenic acid flaxseed (Linum usitatissimum): some nutritional properties in humans. Br J Nutr 69, 443453.

6 JR Romans , RC Johnson , DM Wulf , GW Libal & WJ Costello (1995) Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork: I. Dietary level of flaxseed. J Anim Sci 73, 19821986.

7 M Axelson , J Sjovall , BE Gustafsson & KD Setchell (1982) Origin of lignans in mammals and identification of a precursor from plants. Nature 298, 659660.

9 L Wang , J Chen & LU Thompson (2005) The inhibitory effect of flaxseed on the growth and metastasis of estrogen receptor negative human breast cancer xenografts is attributed to both its lignan and oil components. Int J Cancer 116, 793798.

10 K Prasad (1997) Hydroxyl radical-scavenging property of secoisolariciresinol diglucoside (SDG) isolated from flax-seed. Mol Cell Biochem 168, 117123.

11 K Prasad (1999) Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99, 13551362.

12 D Li , JA Yee , LU Thompson & L Yan (1999) Dietary supplementation with secoisolariciresinol diglycoside (SDG) reduces experimental metastasis of melanoma cells in mice. Cancer Lett 142, 9196.

13 K Prasad (2001) Secoisolariciresinol diglucoside from flaxseed delays the development of type 2 diabetes in Zucker rat. J Lab Clin Med 138, 3239.

14 K Prasad , SV Mantha , AD Muir & ND Westcott (2000) Protective effect of secoisolariciresinol diglucoside against streptozotocin-induced diabetes and its mechanism. Mol Cell Biochem 206, 141149.

16 Y Matsuzawa , T Funahashi & T Nakamura (1999) Molecular mechanism of metabolic syndrome X: contribution of adipocytokines adipocyte-derived bioactive substances. Ann N Y Acad Sci 892, 146154.

17 T Yamauchi , J Kamon , H Waki , (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 7, 941946.

18 P Johnsson , A Kamal-Eldin , LN Lundgren & P Aman (2000) HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds. J Agric Food Chem 48, 52165219.

19 J Fritsche , R Angoelal & M Dachtler (2002) On-line liquid-chromatography-nuclear magnetic resonance spectroscopy-mass spectrometry coupling for the separation and characterization of secoisolariciresinol diglucoside isomers in flaxseed. J Chromatogr 972, 195203.

21 T Shoji , M Kobori , H Shinmoto , A Yanagida , T Kanda & T Tsushida (2000) Inhibitory effects of apple polyphenols on differentiation of 3T3-L1 cells into adipocytes. Food Sci Technol Res 6, 119121.

22 DJ Dyck , GJ Heigenhauser & CR Bruce (2006) The role of adipokines as regulators of skeletal muscle fatty acid metabolism and insulin sensitivity. Acta Physiol (Oxf) 186, 516.

23 M Armoni , N Kritz , C Harel , F Bar-Yoseph , H Chen , MJ Quon & E Karnieli (2003) Peroxisome proliferator-activated receptor-gamma represses GLUT4 promoter activity in primary adipocytes, and rosiglitazone alleviates this effect. J Biol Chem 278, 3061430623.

24 AN Hollenberg , VS Susulic , JP Madura , B Zhang , DE Moller , P Tontonoz , P Sarraf , BM Spiegelman & BB Lowell (1997) Functional antagonism between CCAAT/enhancer binding protein-alpha and peroxisome proliferator-activated receptor-gamma on the leptin promoter. J Biol Chem 272, 52835290.

25 M Iwaki , M Matsuda , N Maeda , T Funahashi , Y Matsuzawa , M Makishima & I Shimomura (2003) Induction of adiponectin, a fat-derived antidiabetic and antiatherogenic factor, by nuclear receptors. Diabetes 52, 16551663.

26 BP Kota , TH Huang & BD Roufogalis (2005) An overview on biological mechanisms of PPARs. Pharmacol Res 51, 8594.

27 N Marx , H Duez , JC Fruchart & B Staels (2004) Peroxisome proliferator-activated receptors and atherogenesis: regulators of gene expression in vascular cells. Circ Res 94, 11681178.

30 SJ Bhathena , AA Ali , C Haudenschild , (2003) Dietary flaxseed meal is more protective than soy protein concentrate against hypertriglyceridemia and steatosis of liver in an animal model of obesity. J Am Coll Nutr 22, 157164.

31 HJ Kim , M Takahashi & O Ezaki (1999) Fish oil feeding decreases mature sterol regulatory element-binding protein 1 (SREBP-1) by down-regulation of SREBP-1c mRNA in mouse liver. A possible mechanism for down-regulation of lipogenic enzyme mRNAs. J Biol Chem 274, 2589225898.

32 H Fujita , H Fujishima , J Koshimura , M Hosoba , N Yoshioka , T Shimotomai , T Morii , T Narita , M Kakei & S Ito (2005) Effects of antidiabetic treatment with metformin and insulin on serum and adipose tissue adiponectin levels in db/db mice. Endocr J 52, 427433.

33 NF Brown , JK Hill , V Esser , JL Kirkland , BE Corkey , DW Foster & JD McGarry (1997) Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J 327, Pt 1, 225231.

35 T Clavel , G Henderson , W Engst , J Dore & M Blaut (2006) Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside. FEMS Microbiol Ecol 55, 471478.

36 JM Lehmann , LB Moore , TA Smith-Oliver , WO Wilkison , TM Willson & SA Kliewer (1995) An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). J Biol Chem 270, 1295312956.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 6
Total number of PDF views: 108 *
Loading metrics...

Abstract views

Total abstract views: 233 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 25th July 2017. This data will be updated every 24 hours.