Skip to main content Accessibility help
×
Home

Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats

  • Brigitta Kleessen (a1), Ludger Hartmann (a1) and Michael Blaut (a1)

Abstract

The effects of fructans in the diet on the mucosal morphometry (height of villi, depth of the crypts, number of goblet cells), the thickness of the epithelial mucus layer and the histochemical composition of intestinal mucosubstances in the distal jejunum and the distal colon were investigated by comparing germ-free (GF) rats, rats harbouring Bacteroides vulgatus and Bifidobacterium longum (diassociated (DA) rats), and rats with a human faecal flora (HFA). The rats were fed either a commercial standard diet (ST) or ST + (50 g oligofructose (OF)–long-chain inulin (lcIN))/kg. Changes in total bacteria, bifidobacteria and BacteroidesPrevotella in response to feeding these diets were investigated by fluorescent in situ hybridization with 16S rRNA-targeted probes both in intestinal contents (lumen bacteria) and tissue sections (mucosa-associated bacteria). The OF–lcIN-containing diet resulted in higher villi and deeper crypts in bacteria-associated, but not in GF rats. In DA and HFA rats, the colonic epithelial mucus layer was thicker and the numbers of the goblet cells were greater than in GF rats. These effects were enhanced by the OF–lcIN-containing diet. In both dietary groups, bacterial colonization of GF rats caused an increase in neutral mucins in the distal jejunum and colon. Bacteria-associated rats had more acidic mucins in the colon than GF rats, and the OF–lcIN-containing diet stimulated sulfomucins as the predominant type of acidic mucins, while sialomucins dominated in the ST-fed groups. The number of mucosa-associated bifidobacteria detected in the colon of DA and HFA rats was greater with OF–lcIN than ST (4·9 and 5·4 v. 3·5 and 4·0 log10/mm2 mucosal surface respectively), whereas the number of luminal bifidobacteria was only affected by fructans in DA rats. Bacteroides did not differ between the groups. The stabilisation of the gut mucosal barrier, either by changes in the mucosal architecture itself, in released mucins or by stimulation of mucosal bifidobacteria with fructans, could become an important topic in the treatment and prophylaxis of gastrointestinal disorders and health maintenance.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding Author: Dr Brigitta Kleessen, fax +49 33200 88 444, email b.kleessen@gmx.de

References

Hide All
Amann, RI, Ludwig, W & Schleifer, K-H (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbial Reviews 59 143169.
Bernet, MF, Brassart, D, Neeser, J-R & Servin, AL (1993) Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen–cell interactions. Applied and Environmental Microbiology 59 41214128.
Brockhausen, I, Schutzbach, J & Kuhns, W (1998) Glycoproteins and their relationship to human disease. Acta Anatomica (Basel) 161 3678.
Campbell, JM, Fahey, GC & Bryan, WW (1997) Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats. Journal of Nutrition 127 130136.
Corfield, AP, Myerscough, N, Gough, M, Brockhausen, I, Schauer, R & Paraskeva, C (1995) Glycosylation pattern of mucins in colonic disease. Biochemical Society Transactions 23 840845.
Deplancke, B & Gaskins, HR (2001) Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. American Journal of Clinical Nutrition 73, Suppl., 1131S1141S.
Djouzi, Z & Andrieux, C (1997) Compared effects of three oligosaccharides on metabolism of intestinal microflora in rats inoculated with a human faecal flora. British Journal of Nutrition 78 313324.
Filipe, MI (1979) Mucins in the human gastrointestinal epithelium: a review. Investigative Cell Pathology 2 195216.
Fontaine, N, Meslin, JC, Lory, S & Andrieux, C (1996) Intestinal mucin distribution in the germ-free rat and in the heteroxenic rat harbouring a human bacterial flora: effect of inulin in the diet. British Journal of Nutrition 75 881892.
Forstner, JF (1978) Intestinal mucins in health and disease. Digestion 17 234263.
Gibson, GR, Beatty, EB, Wang, X & Cummings, JH (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108 975982.
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125 14011412.
Gibson, GR & Wang, X (1994) Regulatory effects of bifidobacteria on the growth of other colonic bacteria. Journal of Applied Bacteriology 74 667674.
He, F, Ouwehand, AC, Hashimoto, H, Isolauri, E, Benno, Y & Salminen, S (2001) Adhesion of Bifidobacterium spp. to human intestinal mucus. Microbiology and Immunology 45 259262.
Kleessen, B, Hartmann, L & Blaut, M (2001) Oligofructose and long-chain inulin: influence on the gut microbial ecology of rats associated with a human faecal flora. British Journal of Nutrition 86 291300.
Kleessen, B, Kroesen, AJ, Buhr, HJ & Blaut, M (2002) Mucosal and invading bacteria in patients with inflammatory bowel disease compared with controls. Scandinavian Journal of Gastroenterology 37 10341041.
Kleessen, B, Noack, J & Blaut, M (1999) Distribution of viable and non-viable bacteria in the gastrointestinal tract of gnotobiotic and conventional rats. Microbial Ecology in Health and Disease 11 218225.
Kleessen, B, Sykura, B, Zunft, H-J & Blaut, M (1997) Effects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. American Journal of Clinical Nutrition 65 13971402.
Korshunov, UM, Sinitsyna, NA, Gindoman, GA & Pinegin, BV (1985) Correction of intestinal microflora in chemotherapeutic dysbacteriosis using bifidobacterial and lactobacterial autologous strains. Journal of Microbiology, Epidemiology and Immunobiology 62, 2025 [in Russian].
Kruse, H-P, Kleessen, B & Blaut, M (1999) Effects of inulin on faecal bifidobacteria in human subjects. British Journal of Nutrition 82 375382.
Laboisse, C, Jarry, A, Branka, JE, Merlin, D, Bou-Hanna, C & Vallette, G (1996) Recent aspects of the regulation of intestinal mucus secretion. Proceedings of the Nutrition Society 55 259264.
Langendijk, PS, Schut, F, Jansen, GJ, Raangs, GC, Kamphuis, GR, Wilkinson, MHF & Welling, GW (1995) Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Applied and Environmental Microbiology 61 30693075.
Macfarlane, S, Cummings, JH & Macfarlane, GT (2000) Bacterial populations on the rectal mucosa in healthy and colitic subjects. Gastroenterology 118, Suppl. 2, A101 (Abstr 678).
Manz, W, Amann, R, Ludwig, W, Vancanneyt, M & Schleifer, K-H (1996) Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142 10971106.
Matsuo, K, Ota, H, Akamatsu, T, Sugiyama, A & Katsuyama, T (1997) Histochemistry of the surface mucous gel layer of the human colon. Gut 40 782789.
Meslin, JC, Andrieux, C, Sakata, T, Beaumatin, P, Bensaada, M, Popot, F, Szylit, O & Durand, M (1993) Effects of galacto-oligosaccharide and bacterial status on mucin distribution in mucosa and on large intestine fermentation in rats. British Journal of Nutrition 69 903912.
Meslin, JC, Fontaine, N & Andrieux, C (1999) Variations of mucin distribution in the rat intestine, caecum and colon: effect of the bacterial flora. Comparative Biochemistry and Physiology A 123 235239.
Poxton, IR, Brown, R, Sawyer, A & Ferguson, A (1997) Mucosa-associated bacterial flora of the human colon. Journal of Medical Microbiology 46 8591.
Prosky, L, Asp, N-G, Schweizer, TF, De Vries, J & Furda, I (1988) Determination of insoluble, soluble, and total dietary fiber in foods and food products: interlaboratory study. Journal of the Association of Analytical Chemists 71 10171023.
Rhodes, JM (1997) Mucins and inflammatory bowel disease. Quarterly Journal of Medicine 90 7982.
Roberfroid, MB, Van Loo, JA & Gibson, GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. Journal of Nutrition 128 1119.
Romeis, R (1989) Polysaccharide and mucous substances. In Microscopic Technique, pp. 439444 [Böck, P, editor]. Munich, Vienna, Baltimore: Urban & Schwarzenberg.
Sakata, T (1987) Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects on fermentable fibre, gut microbes and luminal trophic factors. British Journal of Nutrition 58 95103.
Satchithanandam, S, Vargofcak-Apker, M, Calvert, RJ, Leeds, AR & Cassidy, MM (1990) Alteration of gastrointestinal mucin by fiber feeding in rats. Journal of Nutrition 120 11791184.
Sekine, K, Toida, T, Saito, M, Kuboyama, M & Kawashima, T (1985) A new morphologically characterized cell wall preparation (whole peptidoglucan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Cancer Research 45 13001307.
Schmidt-Wittig, U, Enss, M-L, Coenen, M, Gärtner, K & Hedrich, HJ (1996) Response of rat colonic mucosa to a high fiber diet. Annals of Nutrition and Metabolism 40 343350.
Sghir, A, Chow, JM & Mackie, RI (1998) Continuous culture selection of bifidobacteria and lactobacilli from human faecal samples using fructooligosaccharide as selective substrate. Journal of Applied Microbiology 85 769777.
Sharma, R & Schumacher, U (1995) Morphometric analysis of intestinal mucins under different dietary conditions and gut flora in rats. Digestive Diseases and Sciences 40 25322539.
Sharma, R, Schumacher, U, Ronaasen, V & Coates, M (1995) Rat intestinal mucosal responses to a microbial flora and different diets. Gut 36 209214.
Tappenden, KA, Drozdowski, LA, Thomson, ABR & McBurney, MI (1998) Short-chain fatty acid-supplemented total parenteral nutrition alters intestinal structure, glucose transporter 2 (GLUT2) mRNA and protein, and proglucagon mRNA abundance in normal rats. American Journal of Clinical Nutrition 68 118125.
Van Loo, J, Coussement, P, De Leenheer, L, Hoebregs, H & Smits, G (1995) On the presence of inulin and oligofructose as natural ingredients in the Western diet. Critical Reviews in Food Science and Nutrition 35 525552.
Van Loo, J, Cummings, J, Delzenne, N, Englyst, H, Franck, A, Hopkins, M, Kok, N, Macfarlane, G, Newton, D, Quigley, M, Roberfroid, M, Van Vliet, T & Van den Heuvel, E (1999) Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095). British Journal of Nutrition 81 121132.
Wang, X & Gibson, GR (1993) Effect of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75 373380.
Wilcoxon, F & Wilcox, RA (1964) Some Rapid Approximate Statistical Procedures. New York: Lederle Laboratories.
Zar, JH (1984) Biostatistical Analysis, 2nd ed., New York: Prentice-Hall.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed