Skip to main content
×
Home
    • Aa
    • Aa

Human fasting plasma concentrations of vitamin E and carotenoids, and their association with genetic variants in apo C-III, cholesteryl ester transfer protein, hepatic lipase, intestinal fatty acid binding protein and microsomal triacylglycerol transfer protein

  • Patrick Borel (a1) (a2) (a3), Myriam Moussa (a1) (a2) (a3), Emmanuelle Reboul (a1) (a2) (a3), Bernard Lyan (a4), Catherine Defoort (a1) (a2) (a3), Stéphanie Vincent-Baudry (a1) (a2) (a3), Matthieu Maillot (a1) (a2) (a3), Marguerite Gastaldi (a1) (a2) (a3), Michel Darmon (a1) (a2) (a3), Henri Portugal (a1) (a2) (a3), Denis Lairon (a1) (a2) (a3) and Richard Planells (a1) (a2) (a3)...
Abstract

Plasma concentrations of vitamin E and carotenoids are governed by several factors, including genetic factors. Single nucleotide polymorphisms (SNP) in some genes involved in lipid metabolism have recently been associated with fasting plasma concentrations of these fat-soluble micronutrients. To further investigate the role of genetic factors that modulate the plasma concentrations of these micronutrients, we assessed whether SNP in five candidate genes (apo C-III, CETP, hepatic lipase, I-FABP and MTP) were associated with the plasma concentrations of these micronutrients. Fasting plasma vitamin E and carotenoid concentrations were measured in 129 French Caucasian subjects (forty-eight males and eighty-one females). Candidate SNP were genotyped by PCR amplification followed by restriction fragment length polymorphisms. Plasma γ-tocopherol, α-carotene and β-carotene concentrations were significantly different (P < 0·05) in subjects who carried different SNP variants in hepatic lipase. Plasma α-tocopherol concentrations were significantly different in subjects who had different SNP variants in apo C-III and cholesteryl ester transfer protein (CETP). Plasma lycopene concentrations were significantly different (P < 0·05) in women who had different SNP variants in intestinal fatty acid binding protein (I-FABP). Finally, there was no effect of SNP variants in microsomal TAG transfer protein upon the plasma concentrations of these micronutrients. Most of the observed differences remained significant after the plasma micronutrients were adjusted for plasma TAG and cholesterol. These results suggest that apo C-III, CETP and hepatic lipase play a role in determining the plasma concentrations of tocopherols while hepatic lipase and I-FABP may modulate plasma concentrations of carotenoids.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Human fasting plasma concentrations of vitamin E and carotenoids, and their association with genetic variants in apo C-III, cholesteryl ester transfer protein, hepatic lipase, intestinal fatty acid binding protein and microsomal triacylglycerol transfer protein
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Human fasting plasma concentrations of vitamin E and carotenoids, and their association with genetic variants in apo C-III, cholesteryl ester transfer protein, hepatic lipase, intestinal fatty acid binding protein and microsomal triacylglycerol transfer protein
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Human fasting plasma concentrations of vitamin E and carotenoids, and their association with genetic variants in apo C-III, cholesteryl ester transfer protein, hepatic lipase, intestinal fatty acid binding protein and microsomal triacylglycerol transfer protein
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Patrick Borel, fax +33 4 91 78 21 01, email patrick.borel@univmed.fr
References
Hide All
1Jian L, Du CJ, Lee AH & Binns CW (2005) Do dietary lycopene and other carotenoids protect against prostate cancer? Int J Cancer 113, 10101014.
2Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE & Etherton TD (2002) Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113, 7188.
3Johnson EJ, Chung HY, Caldarella SM & Snodderly DM (2008) The influence of supplemental lutein and docosahexaenoic acid on serum, lipoproteins, and macular pigmentation. Am J Clin Nutr 87, 15211529.
4Borel P (2003) Factors affecting intestinal absorption of highly lipophilic food microconstituents (fat-soluble vitamins, carotenoids and phytosterols). Clin Chem Lab Med 41, 979994.
5Reboul E, Abou L, Mikail C, Ghiringhelli O, Andre M, Gleize B, Kaloustian J, Portugal H, Amiot M & Borel P (2003) Lutein is apparently absorbed by a carrier-mediated transport process in Caco-2 cells. Clin Nutr 22, S103.
6Reboul E, Abou L, Mikail C, Ghiringhelli O, Andre M, Portugal H, Jourdheuil-Rahmani D, Amiot MJ, Lairon D & Borel P (2005) Lutein transport by Caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI). Biochem J 387, 455461.
7Reboul E, Klein A, Bietrix F, Gleize B, Malezet-Desmoulins C, Schneider M, Margotat A, Lagrost L, Collet X & Borel P (2006) Scavenger receptor class B type I (SR-BI) is involved in vitamin E transport across the enterocyte. J Biol Chem 281, 47394745.
8van Bennekum A, Werder M, Thuahnai ST, Han CH, Duong P, Williams DL, Wettstein P, Schulthess G, Phillips MC & Hauser H (2005) Class B scavenger receptor-mediated intestinal absorption of dietary β-carotene and cholesterol. Biochemistry 44, 45174525.
9During A, Dawson HD & Harrison EH (2005) Carotenoid transport is decreased and expression of the lipid transporters SR-BI, NPC1L1, and ABCA1 is downregulated in Caco-2 cells treated with ezetimibe. J Nutr 135, 23052312.
10Anwar K, Iqbal J & Hussain MM (2007) Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption. J Lipid Res 48, 20282038.
11Krinsky NI, Cronwell DG & Oncley JL (1958) The transport of vitamin A and carotenoids in human plasma. Arch Biochem Biophys 73, 233246.
12Romanchik JE, Morel DW & Harrison EH (1995) Distributions of carotenoids and α-tocopherol among lipoproteins do not change when human plasma is incubated in vitro. J Nutr 125, 26102617.
13Massey JB (1984) Kinetics of transfer of α-tocopherol between model and native plasma lipoproteins. Biochim Biophys Acta 793, 387392.
14Traber MG, Lane JC, Lagmay NR & Kayden HJ (1992) Studies on the transfer of tocopherol between lipoproteins. Lipids 27, 657663.
15Kostner GM, Oettl K, Jauhiainen M, Ehnholm C, Esterbauer H & Dieplinger H (1995) Human plasma phospholipid transfer protein accelerates exchange transfer of α-tocopherol between lipoproteins and cells. Biochem J 305, 659667.
16Jiang XC, Tall AR, Qin S, et al. (2002) Phospholipid transfer protein deficiency protects circulating lipoproteins from oxidation due to the enhanced accumulation of vitamin E. J Biol Chem 277, 3185031856.
17Goti D, Reicher H, Malle E, Kostner GM, Panzenboeck U & Sattler W (1998) High-density lipoprotein (HDL3)-associated α-tocopherol is taken up by HepG2 cells via the selective uptake pathway and resecreted with endogenously synthesized apo-lipoprotein B-rich lipoprotein particles. Biochem J 332, 5765.
18Borel P, Moussa M, Reboul E, et al. (2007) Human plasma levels of vitamin E and carotenoids are associated with genetic polymorphisms in genes involved in lipid metabolism. J Nutr 137, 26532659.
19Gomez-Coronado D, Entrala A, Alvarez JJ, Ortega H, Olmos JM, Castro M, Sastre A, Herrera E & Lasuncion MA (2002) Influence of apolipoprotein E polymorphism on plasma vitamin A and vitamin E levels. Eur J Clin Invest 32, 251258.
20Ortega H, Castilla P, Gomez-Coronado D, Garces C, Benavente M, Rodriguez-Artalejo F, de Oya M & Lasuncion MA (2005) Influence of apolipoprotein E genotype on fat-soluble plasma antioxidants in Spanish children. Am J Clin Nutr 81, 624632.
21Fruchart JC & Duriez P (1995) The important role of apolipoprotein C-III in lipoprotein metabolism (article in French). C R Seances Soc Biol Fil 189, 889897.
22Santamarina-Fojo S & Haudenschild C (2000) Role of hepatic and lipoprotein lipase in lipoprotein metabolism and atherosclerosis: studies in transgenic and knockout animal models and somatic gene transfer. Int J Tissue React 22, 3947.
23Besnard P, Niot I, Poirier H, Clement L & Bernard A (2002) New insights into the fatty acid-binding protein (FABP) family in the small intestine. Mol Cell Biochem 239, 139147.
24Yamashita S (2001) Microsomal triglyceride transfer protein (MTP) (article in Japanese). Nippon Rinsho 59, Suppl. 2, 226235.
25Oliveira HCF, Ma LM, Milne R, Marcovina SM, Inazu A, Mabuchi H & Tall AR (1997) Cholesteryl ester transfer protein activity enhances plasma cholesteryl ester formation – studies in CETP transgenic mice and human genetic CETP deficiency. Arterioscler Thromb Vasc Biol 17, 10451052.
26Tyssandier V, Choubert G, Grolier P & Borel P (2002) Carotenoids, mostly the xanthophylls, exchange between plasma lipoproteins. Int J Vitam Nutr Res 72, 300308.
27Vincent S, Gerber M, Bernard MC, et al. (2004) The Medi-RIVAGE study (Mediterranean Diet, Cardiovascular Risks and Gene Polymorphisms): rationale, recruitment, design, dietary intervention and baseline characteristics of participants. Public Health Nutr 7, 531542.
28Vincent-Baudry S, Defoort C, Gerber M, et al. (2005) The Medi-RIVAGE study: reduction of cardiovascular disease risk factors after a 3-mo intervention with a Mediterranean-type diet or a low-fat diet. Am J Clin Nutr 82, 964971.
29Salas J, Jansen S, López-Miranda J, Ordovas JM, et al. (1998) The SstI polymorphism of the apolipoprotein C-III gene determines the insulin response to an oral-glucose-tolerance test after consumption of a diet rich in saturated fats. Am J Clin Nutr 68, 396401.
30Drayna D & Lawn R (1987) Multiple RFLPs at the human cholesteryl ester transfer protein (CETP) locus. Nucleic Acids Res 15, 4698.
31Fumeron F, Betoulle D, Luc G, et al. (1995) Alcohol intake modulates the effect of a polymorphism of the cholesteryl ester transfer protein gene on plasma high density lipoprotein and the risk of myocardial infarction. J Clin Invest 96, 16641671.
32Baier LJ, Sacchettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, Mochizuki H, Bennett PH, Bogardus C & Prochazka M (1995) An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance. J Clin Invest 95, 12811287.
33Karpe F, Lundahl B, Ehrenborg E, Eriksson P & Hamsten A (1998) A common functional polymorphism in the promoter region of the microsomal triglyceride transfer protein gene influences plasma LDL levels. Arterioscler Thromb Vasc Biol 18, 756761.
34Jansen H, Chu G, Ehnholm C, Dallongeville J, Nicaud V & Talmud PJ (1999) The T allele of the hepatic lipase promoter variant C-480T is associated with increased fasting lipids and HDL and increased preprandial and postprandial LpCIII:B: European Atherosclerosis Research Study (EARS) II. Arterioscler Thromb Vasc Biol 19, 303308.
35Doring F, Rimbach G & Lodge JK (2004) In silico search for single nucleotide polymorphisms in genes important in vitamin E homeostasis. IUBMB Life 56, 615620.
36Zampino R, Ingrosso D, Durante-Mangoni E, Capasso R, Tripodi MF, Restivo L, Zappia V, Ruggiero G & Adinolfi LE (2008) Microsomal triglyceride transfer protein (MTP) -493G/T gene polymorphism contributes to fat liver accumulation in HCV genotype 3 infected patients. J Viral Hepat (epublication ahead of print version 15 May 2008).
37Bjorn L, Leren TP, Ose L, Hamsten A & Karpe F (2000) A functional polymorphism in the promoter region of the microsomal triglyceride transfer protein (MTP -493G/T) influences lipoprotein phenotype in familial hypercholesterolemia. Arterioscler Thromb Vasc Biol 20, 17841788.
38Georgopoulos A, Bloomfield H, Collins D, Brousseau ME, Ordovas JM, O'Connor JJ, Robins SJ & Schaefer EJ (2007) Codon 54 polymorphism of the fatty acid binding protein (FABP) 2 gene is associated with increased cardiovascular risk in the dyslipidemic diabetic participants of the Veterans Affairs HDL Intervention Trial (VA-HIT). Atherosclerosis 194, 169174.
39Espino-Montoro A, Barrios-Artillo M, Lopez-Chozas JM, Cayuela A, Stiefel P & Villar J (2003) Influence of polymorphism (RFLP-sstI) at the apolipoprotein C-III gene locus on the lipoprotein metabolism and insulin resistance in essential hypertensive patients. Interaction between gender and genetic polymorphism. Nutr Metab Cardiovasc Dis 13, 194201.
40Frisdal E, Klerkx AH, Le Goff W, Tanck MW, Lagarde JP, Jukema JW, Kastelein JJ, Chapman MJ & Guerin M (2005) Functional interaction between -629C/A, -971G/A and -1337C/T polymorphisms in the CETP gene is a major determinant of promoter activity and plasma CETP concentration in the REGRESS Study. Hum Mol Genet 14, 26072618.
41McCaskie PA, Cadby G, Hung J, McQuillan BM, Chapman CM, Carter KW, Thompson PL, Palmer LJ & Beilby JP (2006) The C-480T hepatic lipase polymorphism is associated with HDL-C but not with risk of coronary heart disease. Clin Genet 70, 114121.
42Botma GJ, Verhoeven AJ & Jansen H (2001) Hepatic lipase promoter activity is reduced by the C-480T and G-216A substitutions present in the common LIPC gene variant, and is increased by upstream stimulatory factor. Atherosclerosis 154, 625632.
43Herbeth B, Gueguen S, Leroy P, Siest G & Visvikis-Siest S (2007) The lipoprotein lipase serine 447 stop polymorphism is associated with altered serum carotenoid concentrations in the Stanislas Family Study. J Am Coll Nutr 26, 655662.
44Behrens WA & Madere R (1985) Transport of α- and γ-tocopherol in human plasma lipoproteins. Nutr Res 5, 167174.
45Esterbauer H, Hell E, Krempler F & Patsch W (1999) Allele-specific differences in apolipoprotein C-III mRNA expression in human liver. Clin Chem 45, 331339.
46Barter P (2000) CETP and atherosclerosis. Arterioscler Thromb Vas Biol 20, 20292031.
47Klein A, Deckert V, Schneider M, et al. (2006) α-Tocopherol modulates phosphatidylserine externalization in erythrocytes: relevance in phospholipid transfer protein-deficient mice. Arterioscler Thromb Vasc Biol 26, 21602167.
48Gastaldi M, Dizière S, Defoort C, Portugal H, Lairon D, Darmon M & Planells R (2007) Sex-specific association of fatty acid binding protein 2 and microsomal triacylglycerol transfer protein variants with response to dietary lipid changes in the 3-mo Medi-RIVAGE primary intervention study. Am J Clin Nutr 86, 16331641.
49Clevidence BA & Bieri JG (1993) Association of carotenoids with human plasma lipoproteins. Methods Enzymol 214, 3346.
50Bone RA, Landrum JT, Hime GW, Cains A & Zamor J (1993) Stereochemistry of the human macular carotenoids. Invest Ophthalmol Vis Sci 34, 20332040.
51Duan LP, Wang HH, Ohashi A & Wang DQ (2006) Role of intestinal sterol transporters Abcg5, Abcg8, and Npc1l1 in cholesterol absorption in mice: gender and age effects. Am J Physiol Gastrointest Liver Physiol 290, G269G276.
52Herron KL, McGrane MM, Waters D, Lofgren IE, Clark RM, Ordovas JM & Fernandez ML (2006) The ABCG5 polymorphism contributes to individual responses to dietary cholesterol and carotenoids in eggs. J Nutr 136, 11611165.
53Henriksson P, Stamberger M, Eriksson M, Rudling M, Diczfalusy U, Berglund L & Angelin B (1989) Oestrogen-induced changes in lipoprotein metabolism: role in prevention of atherosclerosis in the cholesterol-fed rabbit. Eur J Clin Invest 19, 395403.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 10
Total number of PDF views: 169 *
Loading metrics...

Abstract views

Total abstract views: 242 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th October 2017. This data will be updated every 24 hours.