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Impact of palm date consumption on microbiota growth and large intestinal health: a randomised, controlled, cross-over, human intervention study

  • Noura Eid (a1), Hristina Osmanova (a1), Cecile Natchez (a1), Gemma Walton (a2), Adele Costabile (a2), Glenn Gibson (a2), Ian Rowland (a1) and Jeremy P. E. Spencer (a1) (a3)...


The reported inverse association between the intake of plant-based foods and a reduction in the prevalence of colorectal cancer may be partly mediated by interactions between insoluble fibre and (poly)phenols and the intestinal microbiota. In the present study, we assessed the impact of palm date consumption, rich in both polyphenols and fibre, on the growth of colonic microbiota and markers of colon cancer risk in a randomised, controlled, cross-over human intervention study. A total of twenty-two healthy human volunteers were randomly assigned to either a control group (maltodextrin–dextrose, 37·1 g) or an intervention group (seven dates, approximately 50 g). Each arm was of 21 d duration and was separated by a 14-d washout period in a cross-over manner. Changes in the growth of microbiota were assessed by fluorescence in situ hybridisation analysis, whereas SCFA levels were assessed using HPLC. Further, ammonia concentrations, faecal water genotoxicity and anti-proliferation ability were also assessed using different assays, which included cell work and the Comet assay. Accordingly, dietary intakes, anthropometric measurements and bowel movement assessment were also carried out. Although the consumption of dates did not induce significant changes in the growth of select bacterial groups or SCFA, there were significant increases in bowel movements and stool frequency (P<0·01; n 21) and significant reductions in stool ammonia concentration (P<0·05; n 21) after consumption of dates, relative to baseline. Furthermore, date fruit intake significantly reduced genotoxicity in human faecal water relative to control (P<0·01; n 21). Our data indicate that consumption of date fruit may reduce colon cancer risk without inducing changes in the microbiota.


Corresponding author

* Corresponding author: Professor J. P. E. Spencer, fax +44 118 931 0080, email


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1. Baliga, MS, Baliga, BRV, Kandathil, SM, et al. (2011) A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Int 44, 18121822.
2. Eid, NMS, Al-Awadi, B, Vauzour, D, et al. (2013) Effect of cultivar type and ripening on the polyphenol content of date palm fruit. J Agric Food Chem 61, 24532460.
3. Murphy, N, Norat, T, Ferrari, P, et al. (2012) Dietary fibre intake and risks of cancers of the colon and rectum in the European Prospective Investigation into Cancer and Nutrition (EPIC). PLOS ONE 7, e39361.
4. Tuohy, KM, Conterno, L, Gasperotti, M, et al. (2012) Up-regulating the human intestinal microbiome using whole plant foods, polyphenols, and/or fiber. J Agric Food Chem 60, 87768782.
5. Gibson, GR, Scott, KP, Rastall, RA, et al. (2010) Dietary prebiotics: current status and new definition. Food Sci Tech Bull Funct Foods 7, 119.
6. Roberfroid, M, Gibson, GR, Hoyles, L, et al. (2010) Prebiotic effects: metabolic and health benefits. Br J Nutr 104, S1S63.
7. Tuohy, K, Kolida, S, Lustenberger, A, et al. (2001) The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study. Br J Nutr 86, 341348.
8. Tzounis, X, Rodriguez-Mateos, A, Vulevic, J, et al. (2011) Prebiotic evaluation of cocoa-derived flavanols in healthy humans by using a randomized, controlled, double-blind, crossover intervention study. Am J Clin Nutr 93, 6272.
9. Costabile, A, Klinder, A, Fava, F, et al. (2008) Whole-grain wheat breakfast cereal has a prebiotic effect on the human gut microbiota: a double-blind, placebo-controlled, crossover study. Br J Nutr 99, 110120.
10. Queipo-Ortuño, MI, Boto-Ordóñez, M, Murri, M, et al. (2012) Influence of red wine polyphenols and ethanol on the gut microbiota ecology and biochemical biomarkers. Am J Clin Nutr 95, 13231334.
11. Vendrame, S, Guglielmetti, S, Riso, P, et al. (2011) Six-week consumption of a wild blueberry powder drink increases bifidobacteria in the human gut. J Agric Food Chem 59, 1281512820.
12. Carvalho-Wells, AL, Helmolz, K, Nodet, C, et al. (2010) Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study. Br J Nutr 104, 13531356.
13. Costabile, A, Kolida, S, Klinder, A, et al. (2010) A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects. Br J Nutr 104, 10071017.
14. Shinohara, K, Ohashi, Y, Kawasumi, K, et al. (2010) Effect of apple intake on fecal microbiota and metabolites in humans. Anaerobe 16, 510515.
15. Rastall, RA, Gibson, GR, Gill, HS, et al. (2005) Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: an overview of enabling science and potential applications. FEMS Microbiol Ecol 52, 145152.
16. Del Rio, D, Rodriguez-Mateos, A, Spencer, JP, et al. (2013) Dietary (poly)phenolics in human health: structures, bioavailability, and evidence of protective effects against chronic diseases. Antiox Redox Signal 18, 18181892.
17. Lee, SYH, Munerol, B, Pollard, S, et al. (2006) The reaction of flavanols with nitrous acid protects against N-nitrosamine formation and leads to the formation of nitroso derivatives which inhibit cancer cell growth. Free Radic Biol Med 40, 323334.
18. Den Hond, E, Geypens, B & Ghoos, Y (2000) Effect of high performance chicory inulin on constipation. Nutr Res 20, 731736.
19. Ahmed, R, Segal, I & Hassan, H (2000) Fermentation of dietary starch in humans. Am J Gastroenterol 95, 10171020.
20. Zhang, C-R, Aldosari, SA, Vidyasagar, PSPV, et al. (2013) Antioxidant and anti-inflammatory assays confirm bioactive compounds in Ajwa date fruit. J Agric Food Chem 61, 58345840.
21. Bingham, SA, Day, NE, Luben, R, et al. (2003) Dietary fibre in food and protection against colorectal cancer in the European Prospective Investigation into Cancer and Nutrition (EPIC): an observational study. Lancet 361, 14961501.
22. Eid, N, Enani, S, Walton, G, et al. (2014) The impact of date palm fruits and their component polyphenols, on gut microbial ecology, bacterial metabolites and colon cancer cell proliferation. J Nutr Sci 3, e46.
23. Walton, GE, Rastall, RA, Martini, M, et al. (2010) A double-blind, placebo controlled human study investigating the effects of coffee derived manno-oligosaccharides on the faecal microbiota of a healthy adult population. Int J Probiotics and Prebiotics 5, 7584.
24. Daims, H, Stoecker, K & Wagner, M (2005) Fluorescence in situ hybridisation for the detection of prokaryotes. In Molecular Microbial Ecology, pp. 213239 [AM Osborn and CJ Smith, editors]. Abingdon: Bios-Garland.
25. Collins, A (2004) The comet assay for DNA damage and repair. Mol Biotechnol 26, 249261.
26. Costabile, A, Fava, F, Röytiö, H, et al. (2012) Impact of polydextrose on the faecal microbiota: a double-blind, crossover, placebo-controlled feeding study in healthy human subjects. Br J Nutr 108, 471481.
27. Vichai, V & Kirtikara, K (2006) Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protocols 1, 11121116.
28. Solorzano, L (1969) Determination of ammonia in natural waters by the phenol hypochlorite method. Limnol Oceanogr 14, 799801.
29. Eid, N, Walton, G, Costabile, A, et al. (2013) Polyphenols, glucosinolates, dietary fibre and colon cancer: understanding the potential of specific types of fruit and vegetables to reduce bowel cancer progression. Nutr Aging 2, 4567.
30. Kemperman, RA, Bolca, S, Roger, LC, et al. (2010) Novel approaches for analysing gut microbes and dietary polyphenols: challenges and opportunities. Microbiology 156, 32243231.
31. Walton, GE, van den Heuvel, HEG, Kosters, MHW, et al. (2012) A randomised crossover study investigating the effects of galacto-oligosaccharides on the faecal microbiota in men and women over 50 years of age. Br J Nutr 107, 14661475.
32. Gibson, PR, Barrett, JS & Muir, JG (2013) Functional bowel symptoms and diet. Intern Med J 43, 10671074.
33. Sheridan, PO, Bindels, LB, Saulnier, DM, et al. (2013) Can prebiotics and probiotics improve therapeutic outcomes for undernourished individuals? Gut Microbes 5, 7482.
34. Claesson, MJ, Jeffery, IB, Conde, S, et al. (2012) Gut microbiota composition correlates with diet and health in the elderly. Nature 488, 178184.
35. Rock, W, Rosenblat, M, Borochov-Neori, H, et al. (2009) Effects of date (Phoenix dactylifera L., Medjool or Hallawi Variety) consumption by healthy subjects on serum glucose and lipid levels and on serum oxidative status: a pilot study. J Agric Food Chem 57, 80108017.
36. Costabile, A, Klinder, A, Fava, F, et al. (2008) Whole-grain wheat breakfast cereal has a prebiotic effect on the human gut microbiota: a double-blind, placebo-controlled, crossover study. Br J Nutr 99, 110120.
37. Costabile, A, Kolida, S, Klinder, A, et al. (2010) A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects. Br J Nutr 104, 10071017.
38. Carvalho-Wells, AL, Helmolz, K, Nodet, C, et al. (2010) Determination of the in vivo prebiotic potential of a maize-based whole grain breakfast cereal: a human feeding study. Br J Nutr 104, 13531356.
39. Chen, J, Liang, R, Liu, W, et al. (2013) Pectic-oligosaccharides prepared by dynamic high-pressure microfluidization and their in vitro fermentation properties. Carbohydr Polym 91, 175182.
40. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2010) Scientific opinion on establishing food-based dietary guidelines. EFSA J 8, 1460.
41. Saunders, C. (2009) The anti-proliferative effect of different tomato varieties on the human colon adenocarcinoma cells. Biosci Horizons 2, 172179.
42. Corona, G, Deiana, M, Incani, A, et al. (2009) Hydroxytyrosol inhibits the proliferation of human colon adenocarcinoma cells through inhibition of ERK1/2 and cyclin D1. Mol Nutr Food Res 53, 897903.
43. Deiana, M, Corona, G, Incani, A, et al. (2010) Protective effect of simple phenols from extravirgin olive oil against lipid peroxidation in intestinal Caco-2 cells. Food Chem Toxicol 48, 30083016.
44. Rafter, J, Bennett, M, Caderni, G, et al. (2007) Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients. Am J Clin Nutr 85, 488496.
45. Gill, CIR, Haldar, S, Boyd, LA, et al. (2007) Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults. Am J Clin Nutr 85, 504510.
46. Wang, L, Sardo, C & Henry, C (2007) Effect of freeze-dried black raspberries on human colorectal cancer lesions. In AACR Special Conference in Cancer Research, Advances in Colon Cancer Research, B31.
47. Roberts, MC, Millikan, RC, Galanko, JA, et al. (2003) Constipation, laxative use, and colon cancer in a North Carolina population. Am J Gastroenterol 98, 857864.
48. Burkitt, DP (1971) Epidemiology of cancer of the colon and rectum. Cancer 28, 313.
49. Pool-Zobela, BL & Leuchtb, U (1997) Induction of DNA damage by risk factors of colon cancer in human colon cells derived from biopsies. Mutat Res-Fund Mol M 375, 105115.
50. Corona, G, Deiana, M, Incani, A, et al. (2007) Inhibition of p38/CREB phosphorylation and COX-2 expression by olive oil polyphenols underlies their anti-proliferative effects. Biochem Biophys Res Commun 362, 606611.
51. Venturi, M, Hambly, RJ, Glinghammar, B, et al. (1997) Genotoxic activity in human faecal water and the role of bile acids: a study using the alkaline comet assay. Carcinogenesis 18, 23532359.
52. Nyangale, EP, Mottram, DS & Gibson, GR (2012) Gut microbial activity, implications for health and disease: the potential role of metabolite analysis. J Proteome Res 11, 55735585.
53. Larrosa, M, González-Sarrías, A, Yáñez-Gascón, MJ, et al. (2010) Anti-inflammatory properties of a pomegranate extract and its metabolite urolithin-A in a colitis rat model and the effect of colon inflammation on phenolic metabolism. J Nutr Biochem 21, 717725.
54. Rowland, IR, Rumney, CJ, Coutts, JT, et al. (1998) Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis 19, 281285.
55. Chong, MF, George, T, Alimbetov, D, et al. (2013) Impact of the quantity and flavonoid content of fruits and vegetables on markers of intake in adults with an increased risk of cardiovascular disease: the FLAVURS trial. Eur J Nutr 52, 361378.
56. Tuohy, K. (2009) The human microbiome – a therapeutic target for prevention and treatment of chronic disease. Curr Pharm Des 15, 14011402.
57. Vulevic, J, Juric, A, Tzortzis, G, et al. (2013) A mixture of trans-galactooligosaccharides reduces markers of metabolic syndrome and modulates the fecal microbiota and immune function of overweight adults. J Nutr 143, 324331.
58. Fava, F, Gitau, R, Griffin, BA, et al. (2013) The type and quantity of dietary fat and carbohydrate alter faecal microbiome and short-chain fatty acid excretion in a metabolic syndrome ‘at-risk’ population. Int J Obes 37, 216223.
59. Roberfroid, MB, Van Loo, JAE & Gibson, GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 128, 1119.
60. Hold, GL, Pryde, SE, Russell, VJ, et al. (2002) Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis. FEMS Microbiol Ecol 39, 3339.
61. Kotani, A, Miyaguchi, Y, Kohama, M, et al. (2009) Determination of short-chain fatty acids in rat and human feces by high-performance liquid chromatography with electrochemical detection. Anal Sci 25, 10071011.
62. Eeles, RA, Olama, AAA, Benlloch, S, et al. (2013) Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array. Nat Genet 45, 385391.
63. Baldrick, FR, Sung, C, McFadden, K, et al. (2014) The effect of seaweed derived polyphenols on inflammation and oxidative stress in vivo – the SWAFAX study. Proc Nutr Soc 73 (OCE2), 1.
64. Brown, EM, Rowland, IR, Ternan, NG, et al. (2014) Whole plant foods and colon cancer risk. In Diet–Microbe Interactions in the Gut: Effects on Human Health and Disease, 1st ed., pp. 195–207 [K Tuohy and D Del Rio, editors]. London: Academic Press.
65. Tuohy, KM, Fava, F & Viola, R (2014) ‘The way to a man’s heart is through his gut microbiota’ – dietary pro- and prebiotics for the management of cardiovascular risk. Proc Nutr Soc 73, 172185.
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