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Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets

Published online by Cambridge University Press:  08 March 2007

J. A. Gómez ,
M. L. Tejido  and
M. D. Carro
J. A. Gómez
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071, León, Spain
M. L. Tejido
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071, León, Spain
M. D. Carro*
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071, León, Spain
*
*Corresponding author: Dr M. D. Carro, fax +34 987 291311, email DP1MCT@UNILEON.ES
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Abstract

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Two incubation trials were carried out with the rumen-simulation technique (RUSITEC). In each trial, four vessels received a diet of grass hay and concentrate (600 and 400 g/kg DM, respectively; diet F), and the other four were fed a diet composed of concentrate and barley straw (900 and 100 g/kg DM, respectively; Diet C). Vessels were given 20 g of the corresponding diet daily, and half of them were supplemented with disodium malate to achieve a final concentration of 6.55 mM. There were no effects (P>0·05) of malate either on pH or on the daily production of NH3-N, but malate treatment increased (P<0·05) DM, neutral detergent and acid detergent fibre disappearance after 48 h incubation. The daily production of propionate and butyrate increased (P<0·001), and the ratio CH4:volatile fatty acids decreased (P<0·001) by supplementing both diets with malate. Whereas adding malate to the F diet produced an increase in acetate production (P=0·011) and the growth of solid-associated micro-organisms (P=0·037), no effects (P>0·05) were observed for diet C. For both diets, there were no differences (P>0·05) between treatments in the daily flow of liquid-associated micro-organisms measured using 15N as a microbial marker. These results indicate that malate stimulated the in vitro fermentation of both diets by increasing the apparent disappearance of the diet and decreasing the ratio of CH4:volatile fatty acids, but a greater response was observed with diet F. If these results are confirmed in vivo, malate could be used as a feed additive for ruminants fed diets containing medium proportions of forage (i.e. dairy animals) and not only in animals fed high-concentrate diets, as has so far been proposed.

Keywords

MalateRumen fermentationMicrobial protein synthesisRUSITEC
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 4 , April 2005 , pp. 479 - 484
Copyright
Copyright © The Nutrition Society 2005

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