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Introducing inulin-type fructans

  • Marcel B. Roberfroid (a1)

Inulin is a generic term to cover all β(2 → 1) linear fructans. Chicory inulin is a linear β(2 → 1) fructan (degree of polymerisation (DP) 2 to 60; DPav = 12), its partial enzymatic hydrolysis product is oligofructose (DP 2 to 8; DPav = 4), and by applying specific separation technologies a long-chain inulin knownas inulin HP (DP 10 to 60; DPav = 25) can be produced. Finally, a specific product known as oligofructose-enriched inulin is obtained by combining chicorylong-chain inulin and oligofructose. Because of the b-configuration of the anomeric C2 in their fructose monomers, inulin-type fructans resist hydrolysis byintestinal digestive enzymes, they classify as ‘non-digestible’ carbohydrates, and they are dietary fibres. By increasing faecal biomass and water content ofthe stools, they improve bowel habits, but they have characteristic features different from other fibres. They affect gastrointestinal functions not because oftheir physico-chemical properties but rather because of their biochemical and physiological attributes. In the colon, they are rapidly fermented to produceSCFA that are good candidates to explain some of the systemic effects of inulin-type fructans. Fermentation of inulin-type fructans in the large bowel is aselective process; bifidobacteria (and possibly a few other genera) are preferentially stimulated to grow, thus causing significant changes in the compositionof the gut microflora by increasing the number of potentially health-promoting bacteria and reducing the number of potentially harmful species. Both oligofructosead inulin are prebiotic. They also induce changes in colonic epithelium stimulating proliferation in the crypts, increasing the concentration ofpolyamines, changing the profile of mucins, and modulating endocrine as well as immune functions. From a nutrition labelling perspective, inulin-typefructans are not only prebiotic dietary fibres; they are also low-calorie carbohydrates [6·3 kJ/g (1·5 kcal/g)]. Supported by the results of a large numberof animal studies and human nutrition intervention trials, the claim ‘inulin-type fructans enhance calcium and magnesium absorption‘ is scientifically substantiated, but different inulin-type fructans have probably a different efficacy (in terms of effective daily dose), the most active product being the oligofructose-enriched inulin. A series of animal studies demonstrate that inulin-type fructans affect the metabolism of lipids primarily by decreasingtriglyceridaemia because of a reduction in the number of plasma VLDL particles. The human data largely confirm the animal experiments. They demonstratemainly a reduction in triglyceridaemia and only a relatively slight decrease in cholesterolaemia mostly in (slightly) hypertriglyceridaemic conditions. Inulinappears thus eligible for an enhanced function claim related to normalization of blood triacylglycerols. A large number of animal data convincingly showthat inulin-type fructans reduce the risk of colon carcinogenesis and nutrition intervention trials are now performed to test that hypothesis in human subjectsknown to be at risk for polyps and cancer development in the large bowel.

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Corresponding author
*Corresponding author: Professor Marcel B. Roberfroid, fax +32 10 45 93 01, email,
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Apajalahti, JHA, Kettunen, H, Kettunen, A, Holben, WE, Nurminen, PH, Rautanen, N & Mutanen, M (2002) Culture-independent microbial community analysis reveals that inulin in the diet primarily affects previously unknown bacteria in the mouse cecum. Appl Environ Microbiol 68, 49864995.
Berg, RD (1996) The indigenous gastrointestinal microflora. Trends Microbiol 4, 430435.
Bielecka, M, Biedrzycka, E & Majkowska, A (2002a) Selection of probiotics and prebiotics and confirmation of their in vivo effectiveness. Food Res Int 35, 125131.
Bielecka, M, Biedrzycka, E, Majkowska, A, Juskiewicz, J & Wroblewska, M (2002b) Effect of non-digestible oligosaccharides on gut microecosystem in rats. Food Res Int 35, 139144.
Blaut, M, Collins, MD, Welling, GW, Doré, J, Van Loo, J & de Vos, W (2002) Molecular biological methods for studying the gut microbiota: the EU human gut flora project. Br J Nutr 87, S203S211.
Bouhnik, Y, Flourié, B, Riottot, M, Bisetti, N, Gailing, MF, Guibert, A, Bornet, F & Rambaud, JC (1996) Effects of fructooligosaccharides ingestion on fecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans. Nutr Cancer 26, 2129.
Bouhnik, Y, Vahedi, K & Achour, L (1999) Short-chain fructo-oligosaccharide administration dose dependently increases fecal bifidobacteria in healthy humans. J Nutr 129, 113116.
Brichard, S (1997) Influence de mesures nutritionnelles sur l'homéostasie glucidique du rat diabétique. PhD Thesis, Université Catholique de Louvain.
Buddington, KK, Donahoo, JB & Buddington, RK (2002) Dietary oligofructose and inulin protect mice from enteric and systemic pathogens and tumor inducers. J Nutr 132, 472477.
Buddington, RK, Williams, CH, Chen, SC & Witherly, SA (1996) Dietary supplement of Neosugar alters the faecal flora and decreases the activities of some reductive enzymes in human subjects. Am J Clin Nutr 63, 709716.
Cashman, K (2002) Calcium intake, calcium bioavailability and bone health. Br J Nutr 87, Suppl. 2, S169S177.
Coudray, C, Bellanger, J, Castíglia-Delavaud, C, Vermorel, V & Rayssiguier, Y (1997) Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. Eur J Clin Nutr 51, 375380.
Coudray, C, Tressol, JC, Gueux, E & Rayssiguier, Y (2003) Effects of inulin-type fructans of different chain length and type of branching on intestinal absorption of calcium and magnesium in rats. Eur J Nutr 42, 9198.
Coussement, P (1999) Inulin and oligofructose: safe intakes and legal status. J Nutr 129, 1412S1417S.
Cummings, JH (1997) The Large Intestine in Nutrition and Disease Danone Chair Monograph. Brussels: Institut Danone.
Cummings, JH & Macfarlane, GT (1997) Colonic microflora: nutrition and health. Nutrition 13, 476478.
Daubioul, C, De Wispelaere, L, Taper, H & Delzenne, N (2000) Dietary oligofructose lessens hepatic steatosis, but does not prevent hypertriglyceridemia in obese Zucker rats. J Nutr 130, 13141319.
De Leenheer, L (1996) Production and use of inulin: industrial reality with a promising future Carbohydrates as Organic Raw Materials III, 6792 [Van Bekkum, HRöper, HVoragen, F, editors]. New York: VCH Publishing Inc.
Delzenne, N & Roberfroid, MB (1994) Physiological effects of nondigestible oligosaccharides. Lebensm-Wiss Technol 27, 16.
Delzenne, N, Kok, N, Deloyer, P & Dandrifosse, G (2000) Polyamines as mediators of physiological effects of dietary oligofructose in rats. J Nutr 130, 24562460.
Delzenne, NM, Daubioul, C, Neyrinck, M, Lasa, M & Taper, HS (2002) Inulin and oligofructose modulate lipid metabolism in animals: review of biochemical events and future prospects. Br J Nutr 87, Suppl. 2, S255S259.
Demigné, C, Remesy, C & Morand, C (1999) Short chain fatty acids Colonic Microbiota, Nutrition and Health, 5570 [Gibson, GRoberfroid, M, editors]. Dordrecht: Kluwer Academic.
Diplock, AT, Aggett, PJ, Ashwell, M, Bornet, F, Fern, EB & Roberfroid, MB (1999) Scientific concepts of functional foods in Europe: consensus document. Br J Nutr 81, Suppl. 1, S1S28.
Fairweather-Tait, SJ & Johnson, IT (1999) Bioavailability of minerals Colonic Microbiota: Nutrition and Health, 233244 [Gibson, GRRoberfroid, MB, editors]. Dordrecht: Kluwer Academic.
Farnworth, ER (1993) Fructans in human and animal diets Science and Technology of Fructans, 258270 [Suzuki, MChatterton, NJ, editors]. Boca Raton, FL: CRC Press.
Fooks, LJ & Gibson, GR (2002) In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol Ecol 39, 6775.
Gibson, GR (1998) Dietary modulation of human gut microflora using prebiotics. Br J Nutr 80, S209S212.
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125, 14011412.
Gibson, G, Roberfroid, M(editors) (1999) Colonic Microbiota, Nutrition and Health, Dordrecht: Kluwer Academic.
Gibson, GR, Beatty, ER, Wang, X & Cummings, JH (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975982.
Gibson, GR, Probert, HM, Rastall, R, Van Loo, JAE & Roberfroid, MB (2004) Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev 17, 257259.
Griffin, IJ, Hicks, PMD, Heaney, RP & Abrams, SA (2003) Enriched chicory inulin increases calcium absorption in girls with lower calcium absorption. Nutr Res 23, 901909.
Guigoz, Y, Rochat, F, Perruisseau-Carrier, G, Rochat, I & Schiffrin, EJ (2002) Effects of oligosaccharide on the faecal flora and non-specific immune system in elderly people. Nutr Res 22, 1325.
Hanson, LA & Yolken, RH (1999) Probiotics and other Nutritional Factors, and Intestinal Microflora, Philadelphia, PA: Lippincott-Raven.
Harmsen, HJM, Raangs, GC, Franks, AH, Wildeboer-Veloo, CM & Welling, GW (2002) The effect of the prebiotic inulin and the probiotic Bifidobacterium longum on the fecal microflora of healthy volunteers measured by FISH and DGGE. Microb Ecol Health Dis 14, 211219.
Hidaka, H, Eida, T, Takizawa, T, Tokunahga, T & Tashiro, Y (1986) Effects of fructo-oligosaccharides on intestinal flora and human health. Bifidobacteria Microflora 5, 3750.
Hidaka, H, Tashiro, Y & Eida, T (1991) Proliferation of bifidobacteria by oligosaccharides and their useful effect on human health. Bifidobacteria Microflora 10, 6579.
Holloway, L, Moyniham, S & Friedlander, AL (2005) Effects of Raftilose synergy 1 on mineral absorption and markers of bone turnover in postmenopausal women.
Kennefick, S & Cashman, KD (2000) Investigation of an in vitro model for predicting the effect of food components on calcium availability from meals. Int J Food Sci Nutr 51, 4554.
Khöler, H, McCormick, BA & Walker, WA (2003) Bacterial–enterocyte crosstalk: cellular mechanisms in health and disease. J Pediatr Gastroenterol 36, 175185.
Kleessen, B, Sykura, B, Zunft, HJ & Blaut, M (1997) Effects of inulin and lactose on faecal microflora, microbial activity and bowel habit in elderly constipated persons. Am J Clin Nutr 65, 13971402.
Kleessen, B, Hartmann, L & Blaut, M (2001) Oligofructose and long-chain inulin influence the gut microbial ecology of rats associated with a human faecal flora. Br J Nutr 86, 291300.
Kleessen, B, Hartmann, L & Blaut, M (2003) Fructans in the diet cause alterations of intestinal mucosa architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic, rats. Br J Nutr 89, 597606.
Kok, N, Morgan, L, Williams, C, Roberfroid, M, Thissen, JP & Delzenne, N (1998) Insulin, glucagon-like peptide 1, glucose-dependent insulinotropic polypeptide and insulin-like growth factor I as putative mediators of the hypolipidemic effect of oligofructose in rats. J Nutr 128, 10991103.
Kruse, HP, Kleessen, B & Blaut, M (1999) Effects of inulin on faecal bifidobacteria in human subjects. Br J Nutr 82, 375382.
Langlands, SJ, Hopkins, MJ, Coleman, N & Cummings, JH (2004) Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut 53, 16101616.
Letexier, D, Diraison, F & Beylot, M (2003) Addition of inulin to a moderately high-carbohydrates diet reduces hepatic lipogenesis and plasma triacylglycerol concentrations in humans. Am J Clin Nutr 77, 559564.
Macfarlane, S, Cummings, JH & Macfarlane, GT (1999) Bacterial colonization of surfaces in the large intestine Colonic Microbiota, Nutrition and Health, 7188 [Gibson, GRRoberfroid, MB, editors]. Dordrecht: Kluwer Academic.
Mellander, O (1950) The physiologic importance of the casein phosphopeptide calcium salt, II: per oral calcium dosage of infants. Acta Soc Med (Uppsala) 55, 247255.
Menne, E, Guggenbuhl, N & Roberfroid, M (2000) Fn-type chicory inulin hydrolysate has a prebiotic effect in humans. J Nutr 130, 11971199.
Menzies, IS (1974) Absorption of intact oligosaccharides in health and disease. Biochem Soc Trans 2, 10421047.
Mitsuoka, T, Hidaka, H & Eida, T (1987) Effect of fructo-oligosaccharides on intestinal microflora. Die Nahrung 31, 427436.
Moshfegh, AJ, Friday, JE, Goldman, JP, Chug, Ahuga JK (1999) Presence of inulin and oligofructose in the diets of Americans. J Nutr 129, Suppl. 7S, 1407S1411S.
Ohta, A, Sakai, K, Takasaki, M & Tokunaga, T (1999) Evaluation of the action of calcium resorption enhancement of fructooligosaccharides in tablet candies for humans. Health Nutr Food Res 2, 3743.
Oku, T, Tokunaga, T & Hosoya, N (1984) Nondigestibility of a new sweetener ‘Neosugar’ in the rat. J Nutr 114, 15741581.
Perrin, IV, Marchesini, M, Rochat, FC, Schiffrin, EJ & Schilter, B (2003) Oligofructose does not affect the development of type 1 diabetes mellitus induced by dietary proteins in the diabetes-prone BB rat model. Diabetes Nutr Metab 16, 94101.
Perrin, S, Warchol, M, Grill, JP & Schneider, F (2001) Fermentation of fructooligosaccharides and their components by Bifidobacterium infantis ATCC 15967 on batch culture in semi-synthetic medium. J Appl Microbiol 90, 859865.
Poulsen, M, Molck, AM & Jacobsen, BL (2002) Different effects of short- and long-chained fructans on large intestinal physiology and carcinogen-induced aberrant crypt foci in rats. Nutr Cancer 42, 194205.
Poxton, IR, Brown, R, Sawyer, A & Fergusson, A (1997) Mucosa-associated bacterial flora of the human colon. J Med Microbiol 46, 8591.
Quemener, B, Thibault, JF & Coussement, P (1994) Determination of inulin and oligofructose in food products and integration in the AOAC method for the measurement of total dietary fibre. Lebensm-Wiss Technol 27, 125132.
Rao, V (2001) The prebiotic properties of oligofructose at low intake levels. Nutr Res 21, 843848.
Reddy, BS, Hamid, R & Rao, CV (1997) Effect of dietary oligofructose and inulin on colonic preneoplastic aberrant crypt foci inhibition. Carcinogenesis 18, 13711374.
Roberfroid, M (1993) Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. CRC Crit Rev Food Sci Nutr 33, 103148.
Roberfroid, MB (1998) Prebiotics and synbiotics: concepts and nutritional properties. Br J Nutr 80, S197S202.
Roberfroid, M (2002) Functional foods: concepts and application to inulin and oligofructose. Br J Nutr 87 Suppl. 2 S139S143.
Roberfroid, M (2004) Inulin-type Fructans as Functional Food Ingredients, Boca Raton, FL: CRC Press.
Roberfroid, MB (2004) Prebiotics: the concept revised. J Nutr.
Roberfroid, MB & Delzenne, N (1998) Dietary fructans. Annu Rev Nutr 18, 117143.
Roberfroid, MB & Slavin, J (2000) Nondigestible oligosaccharides. Crit Rev Food Sci Nutr 40, 461480.
Roberfroid, MB, Van Loo, JAE & Gibson, GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis product. J Nutr 128, 1119.
Roberfroid, M, Champ, M & Gibson, G (2002) Nutritional and health benefits of inulin and oligofructose. Br J Nutr 87 Suppl. 2 S139S311.
Rowland, IR (1988) Interactions of the gut microflora and the host in toxicology. Toxicol Pathol 16, 147153.
Rycroft, CE, Jones, MR, Gibson, GR & Rastall, RA (2001) A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J Appl Microbiol 91, 878887.
Saavedra, JM & Tschernia, A (2002) Human studies with probiotics and prebiotics: clinical implications. Br J Nutr 87 Suppl. 2 S241S246.
Saavedra, JM, Tschernia, A, Moore, N, Abi-Hanna, A, Coletta, F & Emenheiser, C (1999) Gastrointestinal function in infants consuming a weaning food supplemented with oligofructose. J Paediatr Gastroenterol Nutr 29 A95.
Salminen, S, Bouley, C, Boutron-Ruault, MC (1998) Functional food science and gastrointestinal physiology and function. Br J Nutr 80, Suppl. 1, S147S171.
Sano, T (1986) Effects of Neosugar on constipation, intestinal microflora, and gall bladder contraction in diabetics.In Proceedings of the Third Neosugar Research Conference 109117Tokyo: Meiji-Seika Publications.
Stürup, S, Hansen, M & Molgaard, C (1997) Measurement of 44 Ca, 43 Ca and 42 Ca 43 Ca isotopic ratios in urine using high resolution inductively coupled plasma mass spectrometry. J Anal At Spectrom 12, 919923.
Tahiri, M, Tressol, JC & Arnaud, J (2003) Effect of short-chain fructooligosaccharides on intestinal calcium absorption and calcium status in postmenopausal women: a stable-isotope study. Am J Clin Nutr 77, 449457.
Takahashi, Y (1986) Effects of fructo-oligosaccharides in the chronic-failure patient.In Proceedings of the Third Neosugar Research Conference, 2130Tokyo: Meiji-Seika Publications.
Taper, HS & Roberfroid, MB (1999) Influence of inulin and oligofructose on breast cancer and tumour growth. J Nutr 129, 1488S1491S.
Taper, HS & Roberfroid, MB (2000) Inhibitory effect of dietary inulin or oligofructose on the development of cancer metastases. Anticancer Res 20, 42914294.
Taper, HS & Roberfroid, MB (2002a) Non-toxic potentiation of cancer radiotherapy by dietary oligofructose and inulin. Anticancer Res 22, 33193324.
Taper, HS & Roberfroid, MB (2002b) Inulin/oligofructose, and anticancer therapy. Br J Nutr 87, Suppl. 2, S283S286.
Taper, HS, Delzenne, NM & Roberfroid, MB (1997) Growth inhibition of transplantable mouse tumors by non-digestible carbohydrates. Int J Cancer 71, 11091112.
Taper, HS, Lemort, C & Roberfroid, MB (1998) Inhibition effect of dietary inulin and oligofructose on the growth of transplantable mouse tumor. Anticancer Res 18, 41234126.
Teuri, U, Kärkkäinen, M, Lamberg-Allardt, C & Korpela, R (1999) Addition of inulin to breakfast does not acutely affect serum ionised calcium and parathyroid hormone concentrations. Ann Nutr Metab 43, 356364.
Tuohy, KM, Finlay, RK, Wynne, AG & Gibson, GR (2001a) A human volunteer study on the prebiotic effects of HP-inulin – faecal bacteria enumerated using fluorescent in situ hybridization (FISH). Ecol Environ Microbiol 7, 113118.
Tuohy, KM, Kolida, S, Lustenberger, A & Gibson, GR (2001b) The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructooligosaccharides – a human volunteer study. Br J Nutr 86, 341348.
Uenishi, K, Ohta, A, Fukushima, Y & Kagawa, Y (2002) Effect of a malt drink containing fructooligosaccharides on calcium absorption and safety of long term administration. Jpn J Nutr Diet 60, 1118.
Van den, Heuvel, EG, Schaafsma, G, Muijs, T, van & Dokkum, W (1998) Non-digestible oligosaccharides do not interfere with calcium and nonheme-iron absorption in young, healthy men. Am J Clin Nutr 67, 445451.
Van Loo, J & Jonkers, N (2001) Evaluation in human volunteers of the potential anticarcinogenic activities of novel nutritional concepts: prebiotics, probiotics and synbiotics (the SYNCAN project QLK1-1999-00346). Nutr Metab Cardiovasc Dis 11, Suppl.8793.
Van Loo, J, Coussement, P, De Leenheer, L, Hoebregs, H & Smits, G (1995) On the presence of inulin and oligofructose as natural ingredients in the Western diet. Crit Rev Food Sci Nutr 35, 525552.
Waterhouse, AL & Chatterton, NJ (1993) Glossary of fructan terms Science and Technology of Fructans, 27 [Suzuki, MChatterton, NJ, editors]. Boca Raton, FL:CRC Press.
Weaver, CM & Liebman, M (2002) Biomarkers of bone health appropriate for evaluating functional foods designed to reduce risk of osteoporosis. Br J Nutr 88, Suppl. 2, S225S232.
Whiting, SJ & Wood, RJ (1997) Adverse effects of high calcium diets in humans. Nutr Rev 55, 19.
Williams, CH, Witherly, SA & Buddington, RK (1994) Influence of dietary Neosugar on selected bacteria groups of the human fecal microbiota. Microb Ecol Health Dis 7, 9197.
Wolf, BW, Firkins, JL & Zhang, X (1998) Varying dietary concentrations of fructooligosaccharides affect apparent absorption and balance of minerals in growing rats. Nutr Res 18, 17911806.
Yamada, S (1994) 45 Ca kinetics and balance study. A useful method for analysing the effects of drugs on calcium metabolism.In Pharmacological Approach to Study the Formation and the Resorption Mechanism of Hard Tissues, 7592 [Ogura, H, editors]. Tokyo: EuroAmerica Publishers.
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