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Lifestyle and genetic determinants of folate and vitamin B12 levels in a general adult population

  • Betina H. Thuesen (a1), Lise Lotte N. Husemoen (a1), Lars Ovesen (a2), Torben Jørgensen (a1), Mogens Fenger (a3) and Allan Linneberg (a1)
  • DOI: http://dx.doi.org/10.1017/S0007114509992947
  • Published online: 08 December 2009
Abstract

Danish legislation regarding food fortification has been very restrictive resulting in few fortified food items on the Danish market. Folate and vitamin B12 deficiency is thought to be common due to inadequate intakes but little is known about the actual prevalence of low serum folate and vitamin B12 in the general population. The aim of the present study was to evaluate the folate and vitamin B12 status of Danish adults and to investigate associations between vitamin status and distinct lifestyle and genetic factors. The study included a random sample of 6784 individuals aged 30–60 years. Information on lifestyle factors was obtained by questionnaires and blood samples were analysed for serum folate and vitamin B12 concentrations and several genetic polymorphisms. The overall prevalence of low serum folate ( < 6·8 nmol/l) was 31·4 %. Low serum folate was more common among men than women and the prevalence was lower with increasing age. Low serum folate was associated with smoking, low alcohol intake, high coffee intake, unhealthy diet, and the TT genotype of the methylenetetrahydrofolate reductase (MTHFR)-C677T polymorphism. The overall prevalence of low serum vitamin B12 ( < 148 pmol/l) was 4·7 %. Low serum vitamin B12 was significantly associated with female sex, high coffee intake, low folate status, and the TT genotype of the MTHFR-C677T polymorphism. In conclusion, low serum folate was present in almost a third of the adult population in the present study and was associated with several lifestyle factors whereas low serum concentrations of vitamin B12 were less common and only found to be associated with a few lifestyle factors.

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Corresponding author
*Corresponding author: Dr Betina H. Thuesen, fax +45 43233977, email beheth01@glo.region.dk
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2RA Dhonukshe-Rutten , JH de Vries , A de Bree , (2009) Dietary intake and status of folate and vitamin B12 and their association with homocysteine and cardiovascular disease in European populations. Eur J Clin Nutr 63, 1830.

4E Reynolds (2006) Vitamin B12, folic acid, and the nervous system. Lancet Neurol 5, 949960.

5MA Sanjoaquin , N Allen , E Couto , (2005) Folate intake and colorectal cancer risk: a meta-analytical approach. Int J Cancer 113, 825828.

7R Garcia-Closas , X Castellsague , X Bosch , (2005) The role of diet and nutrition in cervical carcinogenesis: a review of recent evidence. Int J Cancer 117, 629637.

8M Lucock (2000) Folic acid: nutritional biochemistry, molecular biology, and role in disease processes. Mol Genet Metab 71, 121138.

9R Banerjee (2006) B12 trafficking in mammals: a case for coenzyme escort service. ACS Chem Biol 1, 149159.

10T Jørgensen , K Borch-Johnsen , TF Thomsen , (2003) A randomized non-pharmacological intervention study for prevention of ischaemic heart disease: baseline results Inter99. Eur J Cardiovasc Prev Rehabil 10, 377386.

13SL von Huth , K Borch-Johnsen & T Jorgensen (2007) Commuting physical activity is favourably associated with biological risk factors for cardiovascular disease. Eur J Epidemiol 22, 771779.

14U Toft , LH Kristoffersen , C Lau , (2007) The Dietary Quality Score: validation and association with cardiovascular risk factors: the Inter99 study. Eur J Clin Nutr 61, 270278.

15U Toft , L Kristoffersen , S Ladelund , (2008) Relative validity of a food frequency questionnaire used in the Inter99 study. Eur J Clin Nutr 62, 10381046.

16TJ Cole (2000) Sympercents: symmetric percentage differences on the 100 log(e) scale simplify the presentation of log transformed data. Stat Med 19, 31093125.

17S Ebrahim & SG Davey (2008) Mendelian randomization: can genetic epidemiology help redress the failures of observational epidemiology? Hum Genet 123, 1533.

18BS Drammeh , RL Schleicher , CM Pfeiffer , (2008) Effects of delayed sample processing and freezing on serum concentrations of selected nutritional indicators. Clin Chem 54, 18831891.

19MC Ocke , J Schrijver , GL Obermann-de Boer , (1995) Stability of blood (pro)vitamins during four years of storage at − 20 degrees C: consequences for epidemiologic research. J Clin Epidemiol 48, 10771085.

20R Hannisdal , PM Ueland , SJ Eussen , (2009) Analytical recovery of folate degradation products formed in human serum and plasma at room temperature. J Nutr 139, 14151418.

22LL Husemoen , TF Thomsen , M Fenger , (2004) Effect of lifestyle factors on plasma total homocysteine concentrations in relation to MTHFR(C677T) genotype. Inter99 (7). Eur J Clin Nutr 58, 11421150.

23R Green (2008) Indicators for assessing folate and vitamin B12 status and for monitoring the efficacy of intervention strategies. Food Nutr Bull 29, Suppl. 2, S52S63.

26CI Vardavas , MK Linardakis , CM Hatzis , (2008) Smoking status in relation to serum folate and dietary vitamin intake. Tob Induc Dis 4, 8.

27A de Bree , WM Verschuren , D Kromhout , (2002) Homocysteine determinants and the evidence to what extent homocysteine determines the risk of coronary heart disease. Pharmacol Rev 54, 599618.

28A Ulvik , SE Vollset , G Hoff , (2008) Coffee consumption and circulating B-vitamins in healthy middle-aged men and women. Clin Chem 54, 14891496.

32A Gibson , JV Woodside , IS Young , (2008) Alcohol increases homocysteine and reduces B vitamin concentration in healthy male volunteers – a randomized, crossover intervention study. QJM 101, 881887.

33MS van der Gaag , JB Ubbink , P Sillanaukee , (2000) Effect of consumption of red wine, spirits, and beer on serum homocysteine. Lancet 355, 1522.

39S Hustad , O Midttun , J Schneede , (2007) The methylenetetrahydrofolate reductase 677C->T polymorphism as a modulator of a B vitamin network with major effects on homocysteine metabolism. Am J Hum Genet 80, 846855.

40K Nishio , Y Goto , T Kondo , (2008) Serum folate and methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism adjusted for folate intake. J Epidemiol 18, 125131.

42J Al-Tahan , R Sola , JR Ruiz , (2008) Methylenetetrahydrofolate reductase 677CT polymorphism and cobalamin, folate, and homocysteine status in Spanish adolescents. Ann Nutr Metab 52, 315321.

43RM Gueant-Rodriguez , C Rendeli , B Namour , (2003) Transcobalamin and methionine synthase reductase mutated polymorphisms aggravate the risk of neural tube defects in humans. Neurosci Lett 344, 189192.

44M Candito , R Rivet , B Herbeth , (2008) Nutritional and genetic determinants of vitamin B and homocysteine metabolisms in neural tube defects: a multicenter case-control study. Am J Med Genet A 146A, 11281133.

45JL Gueant , NW Chabi , RM Gueant-Rodriguez , (2007) Environmental influence on the worldwide prevalence of a 776C->G variant in the transcobalamin gene (TCN2). J Med Genet 44, 363367.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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