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Linoleic acid and arachidonic acid metabolism in human peripheral blood leucocytes: comparison with the rat

Published online by Cambridge University Press:  09 March 2007

Stephen C. Cunnane ,
P. W. Napoleon Keeling ,
Richard P. H. Thompson  and
Michael A. Crawford
Stephen C. Cunnane
Affiliation:
Department of Nutritional Biochemistry, Nuffield Laboratories of Comparative Medicine, Institute of Zoology, London NW 1 4RY
P. W. Napoleon Keeling
Affiliation:
Gastrointestinal Laboratory, The Rayne Institute, St Thomas's Hospital, London SE1 7EH
Richard P. H. Thompson
Affiliation:
Gastrointestinal Laboratory, The Rayne Institute, St Thomas's Hospital, London SE1 7EH
Michael A. Crawford
Affiliation:
Department of Nutritional Biochemistry, Nuffield Laboratories of Comparative Medicine, Institute of Zoology, London NW 1 4RY
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Abstract

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1. Peripheral blood leucocytes from human male volunteers and from male rats were incubated in vitro in the presence of 14C-labelled linoleic acid ([14C]LA) or 3H-labelled arachidonic acid ([3H]AA). The time-course of [14C]LA and [3H]AA incorporation into human leucocyte total lipids was maximal at 80–90% of the initial dose within 20–30 min of dosing the cells.

2. Compared with mixed leucocytes, isolated polymorphonuclear leucocytes were only marginally different in the differential incorporation of [14C]LA and [3H]AA into total lipids.

3. In human leucocytes, [14C]LA was incorporated initially into triglycerides but predominantly into phosphatidylcholine thereafter. In the rat, [14C]LA remained as the free acid (63%), with lesser amounts entering the phospholipids (9%), monoglycerides-diglycerides (12%) and triglycerides (< 1%).

4. Utilization of [14C]LA by the Δ6-desaturase was only a minor route of its metabolism in both human and rat leucocytes.

5. 3H-labelled prostaglandins E2 and F2α accounted for up to 30% of the radioactivity released into the incubation medium from human leucocytes incubated with [3H]AA for 60 min.

6. Stimulation of phagocytosis in the human leucocytes with latex beads or with unopsonized zymosan did not alter the differential incorporation of [14C]LA or [3H]AA into the leucocyte lipid fractions.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 51 , Issue 2 , March 1984 , pp. 209 - 217
Copyright
Copyright © The Nutrition Society 1984

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