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Lipid metabolism of orchiectomised rats was affected by fructose ingestion and the amount of ingested fructose

  • Satoru Makino (a1), Taro Kishida (a1) and Kiyoshi Ebihara (a1)
  • DOI: http://dx.doi.org/10.1017/S0007114511003679
  • Published online: 01 August 2011
Abstract

We examined whether lipid metabolism in orchiectomised (ORX) rats was affected by fructose ingestion and the amount of ingested fructose. Sucrose was used as a fructose source. Sham-operated and ORX rats were fed one of the following three diets for 28 d: a maize starch-based diet without sucrose (SU0), a diet by which half or all of maize starch was replaced by sucrose (SU50 or SU100). Body-weight gain and food intake were increased by sucrose ingestion, but decreased by ORX. Plasma total cholesterol concentration was increased by ORX and dose-dependently by sucrose ingestion. Plasma TAG concentration was decreased by ORX, but was increased dose-dependently by sucrose ingestion. Plasma insulin concentration was decreased by ORX, but was not affected by sucrose ingestion. Liver TAG was increased by sucrose ingestion and ORX; however, liver cholesterol concentration was not affected by sucrose ingestion and ORX. The hepatic activity of cholesterol 7α-hydroxylase 1 was not affected by sucrose ingestion and ORX; however, faecal excretion of bile acids was decreased. The mRNA level of microsomal TAG transfer protein, which is the gene related to hepatic VLDL production, was increased by ORX and sucrose ingestion. The mRNA level of uncoupling protein-1 was decreased by ORX, but not by sucrose ingestion. Plasma insulin concentration tended to correlate with the level of sterol-regulatory element-binding protein-1c mRNA (r 0·747, P = 0·088). These results show that lipid metabolism in ORX rats would be affected by the consumption of fructose-rich sweeteners such as sucrose and high-fructose syrup.

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Corresponding author
*Corresponding author: K. Ebihara, fax +81 89 946 9847, email ebihara@agr.ehime-u.ac.jp
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1JA Gevers Leuven (1994) Sex steroids and lipoprotein metabolism. Pharmacol Ther 64, 99126.

2A Girard , S Madani , F Boukortt , (2006) Fructose-enriched diet modifies antioxidant status and lipid metabolism in spontaneously hypertensive rats. Nutrition 22, 758766.

4JF Guthrie & JF Morton (2000) Food sources of added sweeteners in the diets of Americans. J Am Diet Assoc 100, 4351.

6L Tappy & KA (2010) Metabolic effects of fructose and the worldwide increase in obesity. Physiol Rev 90, 2346.

10MJ Sheltaway & MS Losowsky (1975) Determination of fecal bile acids by an enzymic method. Clin Chem Acta 64, 127132.

11T Ogishima & K Okuda (1986) An improved method for assay of cholesterol 7α-hydroxylase activity. Anal Biochem 158, 228232.

12P Chomczynski & N Sacchi (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem 162, 156159.

13SE Borst & CF Conover (2006) Orchiectomized Fischer 344 male rat models body composition in hypogonadal state. Life Sci 79, 411415.

14RG Erben , J Eberle , K Stahr , (2000) Androgen deficiency induces high turnover osteopenia in aged male rats: a sequential histomorphometric study. J Bone Miner Res 15, 10851098.

15L Asarian & N Geary (2006) Modulation of appetite by gonadal steroid hormones. Phil Trans R Soc B 361, 12511263.

18UR Hengge , K Stocks , H Wiehler , (2003) Double-blind, randomized, placebo-controlled phase III trial of oxymetholone for the treatment of HIV wasting. AIDS 17, 699710.

20G De Pergola (2000) The adipose tissue metabolism: role of testosterone and dehydroepiandrosterone. Int J Obes Relat Metab Disord 24, S59S63.

23L Pinilla , LM Seoane , L Gonzalez , (1999) Regulation of serum leptin levels by gonadal function in rats. Eur J Endocrinol 140, 468473.

24D Castrogiovanni , M Perelló , RC Gaillard , (2003) Modulatory role of testosterone in plasma leptin turnover in rats. Endocrine 22, 203210.

25S Margetic , C Gazzola , GG Pegg , (2002) Leptin: a review of its peripheral actions and interactions. Int J Obes Relat Metab Disord 26, 14071433.

26E Hirsch & M Walsh (1982) Effect of limited access to sucrose on overeating and patterns of feeding. Physiol Behav 29, 129134.

27RB Kanarek & R Marks-Kaufman (1979) Developmental aspects of sucrose-induced obesity in rats. Physiol Behav 23, 881885.

28C Bouchard , JP Després & P Mauriège (1993) Genetic and nongenetic determinants of regional fat distribution. Endocr Rev 14, 7293.

30MD Lane & SH Cha (2009) Effect of glucose and fructose on food intake via malonyl-CoA signaling in the brain. Biochem Biophys Res Commun 382, 15.

31KL Teff , SS Elliott , M Tschöp , (2004) Dietary fructose reduces circulating insulin and leptin, attenuates postprandial suppression of ghrelin, and increases triglycerides in women. J Clin Endocrinol Metab 89, 29632972.

33HN Ginsberg (1995) Synthesis and secretion of apolipoprotein B from cultured liver cells. Curr Opin Lipidol 6, 275280.

34JR Wetterau , LP Aggerbeck , ME Bouma , (1992) Absence of microsomal triglyceride transfer protein in individuals with abetalipoproteinemia. Science 258, 9991001.

35D Simon , MA Charles , K Nahoul , (1997) Association between plasma total testosterone and cardiovascular risk factors in healthy adult men: the telecom study. J Clin Endocrinol Metab 82, 682685.

36AM Traish , R Abdou & KE Kypreos (2009) Androgen deficiency and atherosclerosis: the lipid link. Vascul Pharmacol 51, 303313.

37MH Rudling , OG Eggertsen & B Angelin (1996) Regulation of rat hepatic low density lipoprotein receptors. In vivo stimulation by growth hormone is not mediated by insulin-like growth factor I. J Clin Invest 97, 292299.

38C Améen & J Oscarsson (2003) Sex difference in hepatic microsomal triglyceride transfer protein expression is determined by the growth hormone secretory pattern in the rat. Endocrinology 144, 39143921.

39YN Sinha , FW Selby , UJ Lewis , (1972) Studies of GH secretion in mice by a homologous radioimmunoassay for mouse GH. Endocrinology 91, 784792.

40G Strauch , P Pandos & H Bricaire (1971) Fructose induced growth hormone release. J Clin Endocrinol Metab 32, 582584.

41C Améen , D Lindén , B-M Larsson , (2004) Effects of gender and GH secretory pattern on sterol regulatory element-binding protein-1c and its target genes in rat liver. Am J Physiol Endocrinol Metab 287, E1039E1048.

42M Miyazaki , A Dobrzyn , WC Man , (2004) Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and -independent mechanisms. J Biol Chem 279, 2516425171.

43P Parini , B Angelin & M Rudling (1999) Cholesterol and lipoprotein metabolism in aging: reversal of hypercholesterolemia by growth hormone treatment in old rats. Arterioscl Thromb Vasc Biol 19, 832839.

46M Fields & CG Lewis (1999) Dietary fructose but not starch is responsible for hyperlipidemia associated with copper deficiency in rats: effect of high-fat diet. J Am Coll Nutr 18, 8387.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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