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Modulatory effects of black v. green tea aqueous extract on hyperglycaemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models

  • Gamal Ramadan (a1), Nadia M. El-Beih (a1) and Eman A. Abd El-Ghffar (a1)
  • DOI:
  • Published online: 13 October 2009

Cardiovascular complications are a major cause of morbidity and mortality in patients with diabetes, obesity and the metabolic syndrome. Recently, there has been an increasing interest in tea as a protective agent against CVD. Here, we compared the modulatory effects of two different doses (50 and 100 mg/kg body weight given orally for 28 consecutive days) of black tea aqueous extract (BTE, rich in theaflavins and thearubigins) and green tea aqueous extract (GTE, rich in catechins) on experimentally induced hyperglycaemia, hyperlipidaemia and liver dysfunction by alloxan (which destroys pancreatic β-cells and induces type 1 diabetes) and a cholesterol-rich diet (which induces obesity and type 2 diabetes) in male Wistar albino rats. Both tea extracts significantly alleviated most signs of the metabolic syndrome including hyperglycaemia (resulting from type 1 and 2 diabetes), dyslipidaemia and impairment of liver functions induced by alloxan or the cholesterol-rich diet in the animals. Also, the tea extracts significantly modulated both the severe decrease and increase in body weight induced by alloxan and the high-cholesterol diet, respectively. The modulatory effects obtained here were partial or complete, but significant and dose dependent, and slightly more in GTE in most cases. No harmful effects were detected for tea consumption on all parameters measured, except that the high dose of both tea extracts significantly decreased the spleen weight:body weight ratio and induced lymphopenia. The present study supports the hypothesis that both black and green teas may have beneficial effects against the risks of the metabolic syndrome and CVD as shown in rat models of human obesity and diabetes.

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*Corresponding author: Dr Gamal Ramadan, fax +20 2 26842123, email
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3MS Islam & H Choi (2007) Green tea, anti-diabetic or diabetogenic: a dose response study. Biofactors 29, 4553.

4T Murase , A Nagasawa , J Suzuki , (2002) Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. Int J Obes Relat Metab Disord 26, 14591464.

5S Rossner (2002) Obesity: the disease of the twenty-first century. Int J Obes Relat Metab Disord 26, Suppl. 4, S2S4.

6JK Lin & SY Lin-Shiau (2006) Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols. Mol Nutr Food Res 50, 211217.

8PV Anandh Babu , KE Sabitha & CS Shyamaladevi (2006) Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin-diabetic rats. Clin Exp Pharmacol Physiol 33, 11841189.

9S Klaus , S Pultz , C Thone-Reineke , (2005) Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes (Lond) 29, 615623.

10DL McKay & JB Blumberg (2007) Roles for epigallocatechin gallate in cardiovascular disease and obesity: an introduction. J Am Coll Nutr 26, 362S365S.

11T Yokozawa , T Nakagawa & K Kitani (2002) Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem 50, 35493552.

12H Chen , M Zhang & B Xie (2005) Components and antioxidant activity of polysaccharide conjugate from green tea. Food Chem 90, 1721.

13C Cabrera , R Artacho & R Gimenez (2006) Beneficial effects of green tea – a review. J Am Coll Nutr 25, 7999.

14S Wolfram (2007) Effects of green tea and EGCG on cardiovascular and metabolic health. J Am Coll Nutr 26, 373S388S.

15ME Harbowy & DA Balentine (1997) Tea chemistry. Crit Rev Plant Sci 16, 415448.

16RA Riemersma , CA Rice-Evans , RM Tyrrell , (2001) Tea flavonoids and cardiovascular health. QJM 94, 277282.

20T Csont , G Balogh , C Csonka , (2002) Hyperlipidemia induced by high cholesterol diet inhibits heat shock response in rat hearts. Biochem Biophys Res Commun 290, 15351538.

21A Onody , C Csonka , Z Giricz , (2003) Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiovasc Res 58, 663670.

22P Trinder (1969) Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 6, 2427.

23CS Frings & RT Dunn (1970) A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. Am J Clin Pathol 53, 8991.

24BT Doumas , WA Watson & HG Biggs (1971) Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta 31, 8796.

26J Vikari (1976) Precipitation of plasma lipoproteins by PEG-6000 and its evaluation with electrophoresis and ultracentrifugation. Scand J Clin Lab Invest 36, 265268.

30P Trinder & D Webster (1984) Determination of HDL-cholesterol using 2,4,6-tribromo-3-hydroxybenzoic acid with a commercial CHOD-PAP reagent. Ann Clin Biochem 21, 430433.

32S Reitman & S Frankel (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28, 5663.

35JR Turner & JF Thayer (editors) (2001) Introduction to Analysis of Variance: Design, Analysis and Interpretation. Thousand Oaks, CA: Sage Publications.

38L Czako , A Szabolcs , A Vajda , (2007) Hyperlipidemia induced by a cholesterol-rich diet aggravates necrotizing pancreatitis in rats. Eur J Pharmacol 572, 7481.

39J Butler , N Rodondi , Y Zhu , (2006) Metabolic syndrome and the risk of cardiovascular disease in older adults. J Am Coll Cardiol 47, 15951602.

40MW Knuiman , J Hung , ML Divitini , (2009) Utility of the metabolic syndrome and its components in the prediction of incident cardiovascular disease: a prospective cohort study. Eur J Cardiovasc Prev Rehabil 16, Suppl. 2, 235241.

41LM Thorn , C Forsblom , J Waden , (2009) Metabolic syndrome as a risk factor for cardiovascular disease, mortality, and progression of diabetic nephropathy in type 1 diabetes. Diabetes Care 32, Suppl. 5, 950952.

42M Shimizu , Y Kobayashi , M Suzuki , (2000) Regulation of intestinal glucose transport by tea catechins. Biofactors 13, 6165.

43MJ Kim , GR Ryu , JS Chung , (2003) Protective effects of epicatechin against the toxic effects of streptozotocin on rat pancreatic islets: in vivo and in vitro. Pancreas 26, 292299.

44EM Janle , C Portocarrero , Y Zhu , (2005) Effect of long-term oral administration of green tea extract on weight gain and glucose tolerance in Zucker diabetic (ZDF) rats. J Herb Pharmacother 5, 5565.

45X Zhou , D Wang , P Sun , (2007) Effects of soluble tea polysaccharides on hyperglycemia in alloxan-diabetic mice. J Agric Food Chem 55, 55235528.

46ME Waltner-Law , XL Wang , BK Law , (2002) Epigallocatechin gallate, a constituent of green tea, represses hepatic glucose production. J Biol Chem 277, 3493334940.

47JA Chemler , LT Lock , MA Koffas , (2007) Standardized biosynthesis of flavan-3-ols with effects on pancreatic β-cell insulin secretion. Appl Microbiol Biotechnol 77, 797807.

49KL Kuo , MS Weng , CT Chiang , (2005) Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats. J Agric Food Chem 53, 480489.

50N Matsumoto , K Okushio & Y Hara (1998) Effect of black tea polyphenols on plasma lipids in cholesterol-fed rats. J Nutr Sci Vitaminol (Tokyo) 44, Suppl. 2, 337342.

51TT Yang & MW Koo (2000) Chinese green tea lowers cholesterol level through an increase in fecal lipid excretion. Life Sci 66, 411423.

52CA Bursill , M Abbey & PD Roach (2007) A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed rabbit. Atherosclerosis 193, 8693.

53CL Lin , HC Huang & JK Lin (2007) Theaflavins attenuate hepatic lipid accumulation through activating AMPK in human HepG2 cells. J Lipid Res 48, 23342343.

55DG Raederstorff , MF Schlachter , V Elste , (2003) Effect of EGCG on lipid absorption and plasma lipid levels in rats. J Nutr Biochem 14, 326332.

56SI Koo & SK Noh (2007) Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutr Biochem 18, 179183.

58WL Yee , Q Wang , T Agdinaoay , (2002) Green tea catechins decrease apolipoprotein B-100 secretion from HepG2 cells. Mol Cell Biochem 229, 8592.

59DA Balentine , SA Wiseman & LC Bouwens (1997) The chemistry of tea flavonoids. Crit Rev Food Sci Nutr 37, 693704.

62EB Taylor , WJ Ellingson , JD Lamb , (2005) Long-chain acyl-CoA esters inhibit phosphorylation of AMP-activated protein kinase at threonine-172 by LKB1/STRAD/MO25. Am J Physiol Endocrinol Metab 288, E1055E1061.

63T Murase , K Misawa , S Haramizu , (2009) Catechin-induced activation of the LKB1/AMP-activated protein kinase pathway. Biochem Pharmacol 78, 7884.

64CL Lin & JK Lin (2008) Epigallocatechin gallate (EGCG) attenuates high glucose-induced insulin signaling blockade in human hepG2 hepatoma cells. Mol Nutr Food Res 52, 930939.

65VE Steele , GJ Kelloff , D Balentine , (2000) Comparative chemopreventive mechanisms of green tea, black tea and selected polyphenol extracts measured by in vitro bioassays. Carcinogenesis 21, 6367.

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