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Monocyte chemoattractant protein-1 involvement in the α-tocopherol-induced reduction of atherosclerotic lesions in apolipoprotein E knockout mice

  • Maria C. G. Peluzio (a1), Emir Miguel (a2), Thaís C. Drumond (a2), Giovana C. César (a2), Helton C. Santiago (a2), Mauro M. Teixeira (a2), Enio C. Vieira (a2), Rosa M. E. Arantes (a3) and Jacqueline I. Alvarez-Leite (a2)...

Abstract

We studied the effects of α-tocopheryl acetate supplementation on the development of fatty streaks and its ability to modulate the expression of monocyte chemoattractant protein (MCP)-1 in aortic lesions of apoliprotein E knockout mice. For this purpose, 16-week-old apolipoprotein E knockout mice received α-tocopherol supplemention ((800 mg)/kg diet) for 6 weeks. After this time, total and lipoprotein cholesterol in the serum, hepatic tocopherol, aortic lesion area and MCP-1 (protein and mRNA) expression were analysed. Our present results showed that the dietary supplementation with α-tocopherol did not reduce serum cholesterol nor change lipoprotein profile, but it reduced the area of the aortic lesion by 55%. The reduction in the lesion size was correlated with the reduced expression of MCP-1 mRNA and protein, as detected by real-time quantitative polymerase chain reaction and immunohistochemistry respectively. In conclusion, the results obtained here are relevant to the study of atherosclerosis, as they correlate the effectiveness of vitamin E supplementation in inhibiting the plaque formation with diminished expression of MCP-1 at the aortic lesion.

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Corresponding author

*Corresponding author: Dr Jacqueline I. Alvarez-Leite, fax +55 31 441 5963, email alvarez@ufmg.br

References

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British Journal of Nutrition
  • ISSN: 0007-1145
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