Skip to main content Accesibility Help
×
×
Home

Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers

  • Yixin Hu (a1), Xiudong Liao (a1), Qian Wen (a1), Lin Lu (a1), Liyang Zhang (a1) and Xugang Luo (a1)...
Abstract

To investigate the P absorption and gene expression levels of related co-transporters, type IIb sodium-dependent phosphate co-transporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1) and inorganic phosphate transporter 2 (PiT-2) in the small intestine of broilers, 450 1-d-old Arbor Acres male broilers were randomly allocated to one of three treatments with ten replicate cages of fifteen birds per cage for each treatment in a completely randomised design. Chickens were fed a diet with no added inorganic P (containing 0·06 % non-phytate P (NPP)) or with either 0·21 or 0·44 % NPP for 21 d. Plasma P concentration in the hepatic portal vein, mRNA and protein expression levels of NaPi-IIb, PiT-1 and PiT-2 were determined at 7, 14 and 21 d of age. The results showed that the concentration of P in plasma in the hepatic portal vein increased as dietary NPP increased (P<0·0001). At 14 and 21 d of age, the increase in dietary NPP inhibited (P<0·003) NaPi-IIb mRNA expression level in the duodenum, as well as PiT-1 mRNA and protein expression levels in the ileum, but promoted NaPi-IIb protein expression level (P<0·002) and PiT-2 mRNA and protein expression levels (P<0·04) in the duodenum. These results suggest that NaPi-IIb, PiT-1 and PiT-2 might be important P transporters in the small intestine of broilers. Higher intestinal P absorption may be achieved by up-regulating the protein expression levels of NaPi-IIb and PiT-2 and down-regulating the protein expression of PiT-1.

Copyright
Corresponding author
* Corresponding author: X. Luo, fax +86 10 62810184, email wlysz@263.net
Footnotes
Hide All

These authors contributed equally to the present work.

Footnotes
References
Hide All
1. Berndt, T & Kumar, R (2009) Novel mechanisms in the regulation of phosphorus homeostasis. Physiology (Bethesda) 24, 1725.
2. Liu, SB, Li, SF, Lu, L, et al. (2012) Estimation of standardized phosphorus retention for corn, soybean meal, and corn-soybean meal diet in broilers. Poult Sci 91, 18791885.
3. Liu, SB, Xie, JJ, Lu, L, et al. (2013) Estimation of standardized phosphorus retention for inorganic phosphate sources in broilers. J Anim Sci 91, 37663771.
4. Liu, SB, Li, SF, Lu, L, et al. (2012) Development of a procedure to determine standardized mineral availabilities in soybean meal for broiler chicks. Biol Trace Elem Res 148, 3237.
5. Nakagawa, N & Ghishan, FK (1993) Transport of phosphate by plasma membranes of the jejunum and kidney of the mouse model of hypophosphatemic vitamin D-resistant rickets. Proc Soc Exp Biol Med 203, 328335.
6. Bai, L, Collins, JF & Ghishan, FK (2000) Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine. Am J Physiol Cell Physiol 279, C1135C1143.
7. Hilfiker, H, Hattenhauer, O, Traebert, M, et al. (1998) Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine. Proc Natl Acad Sci U S A 95, 1456414569.
8. Eto, N, Tomita, M & Hayashi, M (2006) NaPi-mediated transcellular permeation is the dominant route in intestinal inorganic phosphate absorption in rats. Drug Metab Pharmacokinet 21, 217221.
9. Yan, F, Angel, R & Ashwell, CM (2007) Characterization of the chicken small intestine type IIb sodium phosphate cotransporter. Poult Sci 86, 6776.
10. Li, J, Yuan, J, Guo, Y, et al. (2012) The influence of dietary calcium and phosphorus imbalance on intestinal napi-iib and calbindin mRNA expression and tibia parameters of broilers. Asian-Australas J Anim Sci 25, 552558.
11. Liu, SB, Hu, YX, Liao, XD, et al. (2016) Kinetics of phosphorus absorption in ligated small intestinal segments of broilers. J Anim Sci 94, 33123320.
12. Giral, H, Caldas, Y, Sutherland, E, et al. (2009) Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate. Am J Physiol Renal Physiol 297, F1466F1475.
13. Olukosi, OA, Kong, C, Fru-Nji, F, et al. (2013) Assessment of a bacterial 6-phytase in the diets of broiler chickens. Poult Sci 92, 21012108.
14. Fang, R, Xiang, Z, Cao, M, et al. (2012) Different phosphate transport in the duodenum and jejunum of chicken response to dietary phosphate adaptation. Asian-Australas J Anim Sci 25, 14571465.
15. Nie, W, Yang, Y, Yuan, J, et al. (2013) Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens. J Anim Sci Biotechnol 4, 34.
16. National Research Council (1994) Nutrient Requirements of Poultry, 9th ed. Washington, DC: National Academies Press.
17. Liu, SB, Liao, XD, Lu, L, et al. (2017) Dietary non-phytate phosphorus requirement of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age. Poult Sci 96, 151159.
18. Jiang, Y, Lu, L, Li, SF, et al. (2016) An optimal dietary non-phytate phosphorus level of broilers fed a conventional corn-soybean meal diet from 4 to 6 weeks of age. Animal 10, 16261634.
19. Bai, SP, Lu, L, Wang, RL, et al. (2012) Manganese source affects manganese transport and gene expression of divalent metal transporter 1 in the small intestine of broilers. Br J Nutr 108, 267276.
20. Thiex, NJ, Manson, H, Anderson, S, et al. (2002) Determination of crude protein in animal feed, forage, grain, and oilseeds by using block digestion with a copper catalyst and steam distillation into boric acid: collaborative study. J AOAC Int 85, 309317.
21. Selle, P, Ravindran, V, Cadogan, D, et al. (1996) The role of microbial phytases in poultry and pig production. Aust Poult Feed Conv 10, 219224.
22. Goldenberg, H & Fernandez, A (1966) Simplified method for the estimation of inorganic phosphorus in body fluids. Clin Chem 12, 871882.
23. Zhu, YW, Lu, L, Li, WX, et al. (2015) Effect of dietary manganese on antioxidant status and expression levels of heat-shock proteins and factors in tissues of laying broiler breeders under normal and high environmental temperatures. Br J Nutr 114, 19651974.
24. Zhang, LY, Li, XF, Liao, XD, et al. (2017) Effect of iron source on iron absorption and gene expression of iron transporters in the ligated duodenal loops of broilers. J Anim Sci 95, 15871597.
25. Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402408.
26. Chow, S-C, Wang, H & Shao, J (2007) Sample Size Calculations in Clinical Research, 2nd ed. Boca Raton, FL: Chapman & Hall/CRC Press.
27. Murer, H & Burckhardt, G (1983) Membrane transport of anions across epithelia of mammalian small intestine and kidney proximal tubule. Rev Physiol Biochem Pharmacol 96, 151.
28. Kavanaugh, MP & Kabat, D (1996) Identification and characterization of a widely expressed phosphate transporter/retrovirus receptor family. Kidney Int 49, 959963.
29. Kavanaugh, MP, Miller, DG, Zhang, W, et al. (1994) Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters. Proc Natl Acad Sci U S A 91, 70717075.
30. Custer, M, Spindler, B, Verrey, F, et al. (1997) Identification of a new gene product (diphor-1) regulated by dietary phosphate. Am J Physiol Renal Physiol 273, F801F806.
31. Tatsumi, S, Segawa, H, Morita, K, et al. (1998) Molecular cloning and hormonal regulation of PiT-1, a sodium-dependent phosphate cotransporter from rat parathyroid glands 1. Endocrinol 139, 16921699.
32. Boyer, CJ, Baines, AD, Beaulieu, É, et al. (1998) Immunodetection of a type III sodium-dependent phosphate cotransporter in tissues and OK cells. Biochim Biophys 1368, 7383.
33. Kaneko, I, Segawa, H, Furutani, J, et al. (2011) Hypophosphatemia in vitamin D receptor null mice: effect of rescue diet on the developmental changes in renal Na+ -dependent phosphate cotransporters. Pflugers Arch 461, 7790.
34. Stauber, A, Radanovic, T, Stange, G, et al. (2005) Regulation of intestinal phosphate transport. II. Metabolic acidosis stimulates Na(+)-dependent phosphate absorption and expression of the Na(+)-P(i) cotransporter NaPi-IIb in small intestine. Am J Physiol Gastrointest Liver Physiol 288, G501G506.
35. Huber, K, Hempel, R & Rodehutscord, M (2006) Adaptation of epithelial sodium-dependent phosphate transport in jejunum and kidney of hens to variations in dietary phosphorus intake. Poult Sci 85, 19801986.
36. Berner, W, Kinne, R & Murer, H (1976) Phosphate transport into brush-border membrane vesicles isolated from rat small intestine. Biochem J 160, 467474.
37. Xu, H, Bai, L, Collins, JF, et al. (2002) Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)(2) vitamin D(3). Am J Physiol Cell Physiol 282, C487C493.
38. Yan, F & Waldroup, P (2006) Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn as influenced by phytase supplementation and vitamin D source. Int J Poult Sci 5, 219228.
39. Han, JC, Yang, XD, Zhang, T, et al. (2009) Effects of 1alpha-hydroxycholecalciferol on growth performance, parameters of tibia and plasma, meat quality, and type IIb sodium phosphate cotransporter gene expression of one- to twenty-one-day-old broilers. Poult Sci 88, 323329.
40. Saddoris, KL, Fleet, JC & Radcliffe, JS (2010) Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. J Nutr 140, 731736.
41. Huber, K, Walter, C, Schröder, B, et al. (2002) Phosphate transport in the duodenum and jejunum of goats and its adaptation by dietary phosphate and calcium. Am J Physiol Regul Integr Comp Physiol 283, R296R302.
42. Hattenhauer, O, Traebert, M, Murer, H, et al. (1999) Regulation of small intestinal Na-P(i) type IIb cotransporter by dietary phosphate intake. Am J Physiol 277, G756G762.
43. Forster, IC, Hernando, N, Biber, J, et al. (2013) Phosphate transporters of the SLC20 and SLC34 families. Mol Aspects Med 34, 386395.
44. Keshavarz, K (1986) The effect of dietary levels of calcium and phosphorus on performance and retention of these nutrients by laying hens. Poult Sci 65, 114121.
45. Rao, SR, Reddy, VR & Reddy, VR (1999) Non-phytin phosphorus requirements of commercial broilers and White Leghorn layers. Anim Feed Sci Technol 80, 110.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed