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Possible adverse effects of frying with vegetable oils

  • Carmen Dobarganes (a1) and Gloria Márquez-Ruiz (a2)

The question of whether heated fats in the diet may be detrimental to health is nowadays of the upmost concern, but finding an answer is not easy and requires careful consideration of different aspects of lipid oxidation. This review is divided into two sections. The first part deals with the nature of the new compounds formed at high temperature in the frying process as well as their occurrence in the diet while the second part focuses on their possible nutritional and physiological effects. Oxidation products present in abused frying fats and oils are the compounds most suspected of impairing the nutritional properties of the oils or involving adverse physiological effects. The recent studies on their health implications include those related to their fate and those focused on their effects in metabolic pathways and the most prevalent diseases.

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*Corresponding author: C. Dobarganes, email
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1Choe, E & Min, DB (2008) Chemistry of deep-fat frying oils. J Food Sci 72, R77R86.
2Sánchez-Muniz, FJ, Bastida, S, Márquez-Ruiz, G, et al. (2007) Effect of heating and frying on oil and food fatty acids. In Fatty Acids in Foods and Their Health Implications, 3rd ed., pp. 511542 [Chow, CK, editor]. Philadelphia, PA: Taylor & Francis Group.
3Velasco, J, Marmesat, S & Dobarganes, MC (2009) Chemistry of frying. In Deep Fat Frying of Foods, pp. 3356 [Sahin, S and Sumnu, G, editors]. Philadelphia, PA: Taylor & Francis Group.
4Márquez-Ruiz, G & Dobarganes, MC (2007) Nutritional and physiological effects of used frying fats. In Deep Frying: Chemistry, Nutrition and Practical Applications, 2nd ed., pp. 173203 [Erickson, MD, editor]. Champaign, IL: AOCS Press.
5Márquez-Ruiz, G, Ruiz-Méndez, MV & Velasco, J (2010) Preventing oxidation during frying of foods. In Oxidation in Foods and Beverages and Antioxidant Applications, pp. 239273 [Decker, EA, Elias, RJ and McClements, DJ, editors]. Cambridge, UK: Woodhead Publishing.
6Dobarganes, MC & Márquez-Ruiz, G (2007) Formation and analysis of oxidized monomeric, dimeric and higher oligomeric triglycerides. In Deep Frying: Chemistry, Nutrition and Practical Applications, 2nd ed., pp. 87110 [Erickson, MD, editor]. Champaign, IL: AOCS Press.
7International Union of Pure and Applied Chemistry (1992) Standard method 2.507: determination of polar compounds in frying fats. In Standard Methods for the Analysis of Oils, Fats and Derivatives, 7th ed.Oxford, UK: Blackwell.
8Firestone, D (2007) Regulation of frying fats and oils. In Deep Frying: Chemistry, Nutrition and Practical Applications, 2nd ed., pp. 373385 [Erickson, MD, editor]. Champaign, IL: AOCS Press.
9Marmesat, S, Morales, A, Velasco, J, et al. (2012) Influence of fatty acid composition on formation of new compounds during thermoxidation and frying. Food Chem 135, 23332339.
10Frankel, EN (2005) Hydroperoxide decomposition. In Lipid Oxidation, 2nd ed., pp. 6798. Bridgwater: The Oily Press.
11Marmesat, S, Velasco, J & Dobarganes, MC (2008) Quantitative determination of epoxyacids, ketoacids and hydroxyacids formed in fats and oils at frying temperatures. J Chromatogr A 1211, 129134.
12Velasco, J, Marmesat, M, Bordeaux, O, et al. (2005) Quantitation of short-chain glycerol-bound compounds in thermoxidized and used frying oils. A monitoring study during thermoxidation of olive and sunflower oils. J Agric Food Chem 53, 40064011.
13Berdeaux, O, Fontagné, S, Sémon, E, et al. (2012) A detailed identification study on high-temperature degradation products of oleic and linoleic acid methyl esters by GC–MS and GC–FTIR. Chem Phys Lipids 165, 338347.
14Dobarganes, MC (1998) Formation and analysis of high molecular-weight compounds in frying fats and oils. OCL 5, 4147.
15Dobarganes, MC & Márquez Ruiz, G (2003) Oxidised fats in foods. Curr Opin Clin Nutr Metab Care 6, 157163.
16Saguy, S & Dana, D (2003) Integrated approach to deep fat frying: engineering, nutrition, health and consumer aspects. J Food Eng 56, 143152.
17Marmesat, S, Rodrigues, E, Velasco, J, et al. (2007) Used frying fats and oils: comparison of rapid tests based on chemical and physical oil properties. Int J Food Sci Technol 42, 601608.
18Márquez-Ruiz, G, Pérez-Camino, MC & Dobarganes, MC (1992) Digestibility of fatty acid monomers, dimers and polymers in the rat. J Am Oil Chem Soc 69, 930934.
19Márquez-Ruiz, G, Pérez-Camino, MC & Dobarganes, MC (1993) Evaluation of hydrolysis and absorption of thermally oxidized olive oil in non-absorbed lipids in the rat. Ann Nutr Metabol 37, 121128.
20Márquez-Ruiz, G, Guevel, G & Dobarganes, MC (1998) Application of chromatographic techniques to evaluate enzymatic hydrolysis of oxidized and polymeric triglycerides by pancreatic lipase ‘in vitro. J Am Oil Chem Soc 75, 119126.
21González-Muñoz, MJ, Bastida, S & Sánchez-Muniz, FJ (1998) Short-term in vivo digestibility of triglyceride polymers, dimers, and monomers of thermoxidized palm olein used in deep-frying. J Agric Food Chem 46, 51885193.
22Olivero-David, R, Sánchez-Muniz, FJ, Bastida, S, et al. (2010) Gastric emptying and short-term digestibility of thermally oxidized sunflower oil used for frying in fasted and nonfasted rats. J Agric Food Chem 58, 92429248.
23Olivero-David, R, Paduano, A, Fogliano, V, et al. (2011) Effect of thermally oxidized oil and fasting status on the short-term digestibility of ketolinoleic acids and total oxidized fatty acids in rats. J Agric Food Chem 59, 46844691.
24Estruch, R, Ros, E, Salas-Salvadó, J, et al. (2013) Primary prevention of cardiovascular disease with a Mediterranean diet. N Engl J Med 368, 12791290.
25Vallverdú-Queralt, A, Rinaldi de Alvarenga, JF, Estruch, R, et al. (2013) Bioactive compounds present in the Mediterranean sofrito. Food Chem 141, 33653372.
26Martínez-González, MA & Bes-Rastrollo, M (2014) Dietary patterns, Mediterranean diet, and cardiovascular disease. Curr Opin Lipid 25, 2026.
27Stott-Miller, M, Neuhouser, ML & Stanford, JL (2013) Consumption of deep-fried foods and risk of prostate cancer. Prostate 73, 960969.
28Dai, Q, Shu, XO, Jin, F, et al. (2002) Consumption of animal foods, cooking methods, and risk of breast cancer. Cancer Epidemiol Biomarkers Prev 11, 801808.
29Galeone, C, Pelucchi, C, Talamini, R, et al. (2005) Role of fried foods on oral/pharyngeal and oesophageal cancers. Br J Cancer 92, 20652069.
30Bosetti, C, Talamini, R, Levi, F, et al. (2002) Fried foods: a risk factor for laryngeal cancer? Br J Cancer 87, 12301233.
31Soriguer, F, Rojo-Martínez, G, Dobarganes, MC, et al. (2003) Hypertension is related to the degradation of dietary frying oils. Am J Clin Nutr 78, 10921097.
32Guallar-Castillón, P, Rodríguez-Artalejo, F, Lopez-García, E, et al. (2007) Intake of fried foods is associated with obesity in the cohort of Spanish adults from the European prospective investigation into cancer and nutrition. Am J Clin Nutr 86, 198205.
33Sayon-Orea, C, Bes-Rastrollo, M, Basterra-Gortari, FJ, et al. (2013) Consumption of fried foods and weight gain in a Mediterranean cohort: the SUN project. Nutr Metab Cardiovasc Dis 23, 144150.
34Donfrancesco, C, Lo, NC, Brignoli, O, et al. (2008) Italian network for obesity and cardiovascular disease surveillance: a pilot project. BMC Fam Pract 9, 53.
35Sayon-Orea, C, Martínez-González, MA, Gea, A, et al. (2014) Consumption of fried foods and risk of metabolic syndrome: the SUN cohort study. Clin Nutr 33, 545549.
36Iqbal, R, Anand, S, Ounpuu, S, et al. (2008) Dietary patterns and the risk of acute myocardial infarction in 52 countries: results of the INTERHEART study. Circulation 118, 19291937.
37Kabagambe, EK, Baylin, A, Siles, X, et al. (2003) Individual saturated fatty acids and nonfatal acute myocardial infarction in Costa Rica. Eur J Clin Nutr 57, 14471457.
38Guallar-Castillon, P, Rodríguez-Artalejo, F, Lopez-García, E, et al. (2012) Consumption of fried foods and risk of coronary heart disease: Spanish cohort of the European prospective investigation into cancer and nutrition study. BMJ 344, e363.
39Chao, PM, Chao, CY, Lin, FJ, et al. (2001) Oxidized frying oil up-regulates hepatic acyl-CoA oxidase and cytochrome 450 4A1 genes in rats and activates PPARα. J Nutr 131, 31663174.
40Sülzle, A, Hirche, F & Eder, K (2004) Thermally oxidized dietary fat upregulates the expression of target genes of PPARα in rat liver. J Nutr 134, 13751383.
41Luci, S, Koènig, B, Giemsa, B, et al. (2007) Feeding of a deep-fried fat causes PPAR activation in the liver of pigs as a non-proliferating species. Br J Nutr 97, 872882.
42Ringseis, E, Gutgesell, A, Dathe, C, et al. (2007) Feeding oxidized fat during pregnancy up-regulates expression of PPARα-responsive genes in the liver of rat foetuses. Lipids Health Dis 6, 6.
43Kammerer, I, Ringseis, R & Eder, K (2011) Feeding a thermally oxidised fat inhibits atherosclerotic plaque formation in the aortic root of LDL receptor-deficient mice. Br J Nutr 105, 190199.
44Ringseis, R & Eder, K (2011) Regulation of genes involved in lipid metabolism by dietary oxidized fat. Mol Nutr Food Res 55, 109121.
45Izaki, Y, Yoshikawa, S & Uchiyama, M (1984) Effect of ingestion of thermally oxidized frying oil on peroxidative criteria in rats. Lipids 19, 324331.
46Liu, JF & Huang, CJ (1995) Tissue α-tocopherol retention in male rats is compromised by feeding diets containing oxidized frying oil. J Nutr 125, 30713080.
47Liu, JF, Lee, WL & Chan, FC (2000) Effects of oxidized frying oil and vitamin C on the hepatic xenobiotic-metabolizing enzyme system of guinea pigs. J Nutr Sci Vitaminol 46, 137140.
48Ringseis, R, Picwek, R & Eder, K (2007) Oxidized fat induces oxidative stress but has no effect on NF-κB-mediated proinflammatory gene transcription in porcine intestinal epithelial cells. Inflamm Res 56, 118125.
49Olivero-David, R, Bastida, S, Schultz, A, et al. (2010) Fasting status and thermally oxidized sunflower oil ingestion affect the intestinal antioxidant enzyme activity and gene expression of male Wistar rats. J Agric Food Chem 58, 24982504.
50Miyashita, K, Takagi, T & Frankel, EN (1990) Preferential hydrolysis of monohydroperoxides of linoleoyl and linolenoyl triacylglycerol by pancreatic lipase. Biochim Biophys Acta 1045, 233238.
51Kanner, J & Lapidot, T (2001) The stomach as a bioreactor: dietary lipid peroxidation in the gastric fluid and the effects of plant-derived antioxidants. Free Radic Biol Med 31, 13881395.
52Kanazawa, K & Ashida, H (1998) Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines. Biochim Biophys Acta 1393, 336348.
53Kanazawa, K & Ashida, H (1998) Dietary hydroperoxides of linoleic acid decompose to aldehydes in stomach before being absorbed into the body. Biochim Biophys Acta 1393, 349361.
54Aw, TY (1998) Determinants of intestinal detoxication of lipid hydroperoxides. Free Radic Res 28, 637646.
55Wingler, K, Muller, C, Schmehl, K, et al. (2000) Gastrointestinal glutathione peroxidase prevents transport of lipid hydroperoxides in CaCo-2 cells. Gastroenterology 119, 420430.
56Muller, C, Friedrich, R, Wingler, K, et al. (2002) Perturbation of lipid metabolism by linoleic acid hydroperoxide in CaCo-2 cells. Biol Chem 383, 637648.
57Aw, TY (2005) Intestinal glutathione: determinant of mucosal peroxide transport, metabolism, and oxidative susceptibility. Toxicol Appl Pharmacol 204, 320328.
58Chisolm, GM & Steinberg, D (2000) The oxidative modification hypothesis of atherogenesis: an overview. Free Radic Biol Med 28, 18151826.
59Cohn, J (2002) Oxidized fat in the diet, postprandial lipaemia and cardiovascular disease. Curr Opin Lipidol 13, 1924.
60Jessup, W, Krithairides, L & Stocker, R (2004) Lipid oxidation in atherogenesis: an overview. Biochem Soc Trans 32, 134138.
61Stocker, R & Keany, JF (2004) Role of oxidative modifications in atherosclerosis. Physiol Rev 84, 13811478.
62Duthie, GG, Wahle, KWJ & James, WPT (1989) Oxidants, antioxidants and cardiovascular disease. Nutr Res Rev 2, 5162.
63Bull, AW, Nigro, ND & Marnett, LJ (1988) Structural requirements for stimulation of colonic cell proliferation by oxidized fatty acids. Cancer Res 48, 17711776.
64Kanazawa, A, Sawa, T, Akaike, T, et al. (2002) Dietary lipid peroxidation products and DNA damage in colon carcinogenesis. Eur J Lipid Sci Technol 104, 439447.
65Tsunada, S, Iwakiri, R, Noda, T, et al. (2003) Chronic exposure to subtoxic levels of peroxidized lipids suppresses mucosal cell turnover in rat small intestine and reversal by glutathione. Digest Dis Sci 48, 210222.
66Tsunada, S, Iwakiri, R, Fujimoto, K, et al. (2003) Chronic lipid hydroperoxide stress suppresses mucosal proliferation in rat intestine: potentiation of ornithine decarboxylase activity by epidermal growth factor. Digest Dis Sci 48, 23332341.
67Wang, TG, Gotoh, Y, Jennings, MH, et al. (2000) Lipid hydroperoxide-induced apoptosis in human colonic CaCo-2 cells is associated with an early loss of cellular redox balance. FASEB J 14, 15671576.
68Jurek, D, Udilova, N, Jozkowicz, A, et al. (2005) Dietary lipid hydroperoxides induce expression of vascular endothelial growth factor (VEGF) in human colorectal tumor cells. FASEB J 19, 9799.
69Yang, MH & Schaich, KM (1996) Factors affecting DNA damage caused by lipid hydroperoxides and aldehydes. Free Radic Biol Med 20, 225236.
70Sawa, T, Akaike, T, Kida, K, et al. (1998) Lipid peroxyl radicals from oxidized oils and heme–iron: implication of a high fat diet in colon carcinogenesis. Cancer Epidemiol Biomarkers Prev 7, 10071012.
71Wilson, R, Fernie, CE, Scrimgeour, CM, et al. (2002) Dietary epoxy fatty acids are absorbed in healthy women. Eur J Clin Invest 32, 7983.
72Wilson, R, Lyall, K, Smyth, L, et al. (2002) Dietary hydroxy fatty acids are absorbed in humans: implications for the measurement of oxidative stress in vivo. Free Radic Biol Med 32, 162168.
73Kammerer, I, Ringseis, R, Biemann, R, et al. (2011) 13-Hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages. Lipids Health Dis 10, 222231.
74Mitchell, LA, Moran, JH & Grant, DF (2002) Linoleic acid, cis-epoxyoctadecanoic acids, and dihydroxyoctadecadienoic acids are toxic to Sf-21 cells in the absence of albumin. Toxicol Lett 126, 187196.
75Moran, JH, Mont, T, Hendrickson, TL, et al. (2001) Defining mechanisms of toxicity for linoleic acid monoepoxides and diols in Sf-21 cells. Chem Res Toxicol 14, 431437.
76Moran, JH, Nowak, G & Grant, DF (2001) Analysis of the toxic effects of linoleic acid, 12,13-cis-epoxyoctadecenoic acid, and 12,13-dihydroxyoctadecenoic acid in rabbit renal cortical mitochondria. Toxicol Appl Pharmacol 172, 150161.
77Slim, R, Hammock, BD, Toborek, M, et al. (2001) The role of methyl-linoleic acid epoxide and diol metabolites in the amplified toxicity of linoleic acid and polychlorinated biphenyls to vascular endothelial cells. Toxicol Appl Pharmacol 171, 184193.
78Perkins, EG & Taubold, R (1978) Nutritional and metabolic studies of noncyclic dimeric fatty acid methyl esters in the rat. J Am Oil Chem Soc 55, 632634.
79Combe, N, Constantin, MJ & Entressangles, B (1981) Lymphatic absorption of nonvolatile oxidation products of heated oils in the rat. Lipids 16, 814.
80Strauss, HJ, Piater, H & Sterner, W (1982) Fütterungsversuche mit dimeren Triglyceriden aus Sojaölraffinat (Feeding Experiments with Dimeric Triglycerides Isolated from Refined Soybean Oil). Fette Seifen Anstrichm 84, 199203.
81Márquez-Ruiz, G & Dobarganes, MC (1995) Assessments on the digestibility of oxidized compounds from [1-14C]-linoleic acid using a combination of chromatographic techniques. J Chromatogr B 675, 18.
82Vonk, RJ, Kalivianakis, M, Minich, DM, et al. (1997) The metabolic importance of unabsorbed dietary lipids in the colon. Scand J Gastroenterol 32, 6567.
83Campbell, S, Stone, WL & Whaley, S (2003) Development of gamma (γ)-tocopherol as a colorectal cancer chemopreventive agent. Crit Rev Oncol Hematol 47, 249259.
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