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Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects

  • Carl J. Hulston (a1), Amelia A. Churnside (a1) and Michelle C. Venables (a2)
Abstract

The purpose of the present study was to determine whether probiotic supplementation (Lactobacillus casei Shirota (LcS)) prevents diet-induced insulin resistance in human subjects. A total of seventeen healthy subjects were randomised to either a probiotic (n 8) or a control (n 9) group. The probiotic group consumed a LcS-fermented milk drink twice daily for 4 weeks, whereas the control group received no supplementation. Subjects maintained their normal diet for the first 3 weeks of the study, after which they consumed a high-fat (65 % of energy), high-energy (50 % increase in energy intake) diet for 7 d. Whole-body insulin sensitivity was assessed by an oral glucose tolerance test conducted before and after overfeeding. Body mass increased by 0·6 (se 0·2) kg in the control group (P< 0·05) and by 0·3 (se 0·2) kg in the probiotic group (P>0·05). Fasting plasma glucose concentrations increased following 7 d of overeating (control group: 5·3 (se 0·1) v. 5·6 (se 0·2) mmol/l before and after overfeeding, respectively, P< 0·05), whereas fasting serum insulin concentrations were maintained in both groups. Glucose AUC values increased by 10 % (from 817 (se 45) to 899 (se 39) mmol/l per 120 min, P< 0·05) and whole-body insulin sensitivity decreased by 27 % (from 5·3 (se 1·4) to 3·9 (se 0·9), P< 0·05) in the control group, whereas normal insulin sensitivity was maintained in the probiotic group (4·4 (se 0·8) and 4·5 (se 0·9) before and after overeating, respectively (P>0·05). These results suggest that probiotic supplementation may be useful in the prevention of diet-induced metabolic diseases such as type 2 diabetes.

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Copyright
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
* Corresponding author: Dr C. J. Hulston, email c.j.hulston@lboro.ac.uk
References
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1 Tremblay F, Lavigne C, Jacques H, et al. (2001) Defective insulin-induced GLUT4 translocation in skeletal muscle of high fat-fed rats is associated with alterations in both Akt/protein kinase B and atypical protein kinase C activities. Diabetes 50, 19011910.
2 Brons C, Jensen CB, Storgaard H, et al. (2009) Impact of short-term high-fat feeding on glucose and insulin metabolism in young healthy men. J Physiol 587, 22872297.
3 Defronzso RA (2004) Dysfunctional fat cells, lipotoxicity and type 2 diabetes. Int J Clin Pract 58, 921.
4 Cani PD, Amar J, Iglesias MA, et al. (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56, 17611772.
5 Cani PD, Bibiloni R, Knauf C, et al. (2008) Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57, 14701481.
6 Cani PD, Possemiers S, van de Wiele T, et al. (2009) Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 58, 10911103.
7 Serino M, Luche E, Gres S, et al. (2012) Metabolic adaptations to a high-fat diet is associated with a change in the gut microbiota. Gut 61, 543553.
8 Larsen N, Vogensen FK, van den Berg FWJ, et al. (2010) Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS ONE 5, e9085.
9 Amar J, Burcelin R, Ruidavets JB, et al. (2008) Energy intake is associated with endotoxemia in apparently healthy men. Am J Clin Nutr 87, 12191223.
10 Pendyala S, Walker JM & Holt PR (2012) A high-fat diet is associated with endotoxemia that originates in the gut. Gastroenterology 142, 11001101.
11 Naito E, Yoshida Y, Makino K, et al. (2011) Beneficial effect of oral administration of Lactobacillus casei strain Shirota on insulin resistance in diet-induced obesity mice. J App Microbiol 110, 650657.
12 Ejtahed HS, Mohtadi-Nia J, Homayouni-Rad A, et al. (2012) Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition 28, 539543.
13 Spanhaak S, Havenaar R & Schaafsma G (1998) The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur J Clin Nutr 52, 899907.
14 Matsumoto K, Takada T, Shimizu K, et al. (2010) Effects of a probiotic fermented milk beverage containing Lactobacillus casei strain Shirota on defecation frequency, intestinal microbiota, and the intestinal environment of healthy individuals with soft stools. J Biosci Bioeng 110, 547552.
15 Nagata S, Asahara T, Ohta T, et al. (2011) Effect of the continuous intake of probiotic-fermented milk containing Lactobacillus casei strain Shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged. Br J Nutr 106, 549556.
16 Matsuda M & DeFronzo RA (1999) Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care 22, 14621470.
17 Kraegen EW, Storlien LH, Jenkins AB, et al. (1989) Chronic exercise compensates for insulin resistance induced by a high-fat diet in rats. Am J Physiol Endocrinol Metab 256, E242E249.
18 Kim C, Youg JH, Park J, et al. (2000) Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats. Am J Physiol Endocrinol Metab 278, E977E984.
19 Lessard SJ, Rivas DA, Chen K, et al. (2007) Tissue-specific effects of rosiglitazone and exercise in the treatment of lipid-induced insulin resistance. Diabetes 56, 18561864.
20 Todd MK, Watt MJ, Le J, et al. (2007) Thiazolidinediones enhanced skeletal muscle triacylglycerol synthesis while protecting against fatty acid-induced inflammation and insulin resistance. Am J Physiol Endocrinol Metab 292, E485E493.
21 Barclay JL, Shostak A, Leliavski A, et al. (2013) High fat diet-induced hyperinsulinemia and tissue-specific insulin resistance in Cry deficient mice. Am J Physiol Endocrinol Metab 304, E1053E1063.
22 Bielohuby M, Sisley S, Sandoval D, et al. (2013) Impaired glucose tolerance in rats fed low-carbohydrate, high-fat diets. Am J Physiol Endocrinol Metab 305, E1059E1070.
23 Wiedemann MSF, Wueest S, Item F, et al. (2013) Adipose tissue inflammation contributes to short-term high-fat diet-induced hepatic insulin resistance. Am J Physiol Endocrinol Metab 305, E388E395.
24 Adochio RL, Leitner JW, Gray K, et al. (2009) Early responses of insulin signalling to high-carbohydrate and high-fat overfeeding. Nutr Metab 6, 37.
25 Cornford AS, Hinko A, Nelson RK, et al. (2013) Rapid development of systemic insulin resistance with overeating is not accompanied by robust changes in skeletal muscle glucose and lipid metabolism. Appl Physiol Nutr Metab 38, 512519.
26 Yu C, Chen Y, Zong H, et al. (2002) Mechanism by which fatty acids inhibit insulin activation of insulin receptor substrate-1 (IRS-1)-associated phosphatidylinositol 3-kinase activity in muscle. J Biol Chem 277, 5023050236.
27 Kleemann R, van Erk M, Verschuren L, et al. (2010) Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance. PloS ONE 5, e8817.
28 Samuel VT & Shulman GI (2012) Mechanisms for insulin resistance: common threads and missing links. Cell 148, 852871.
29 Pussinen PJ, Havulinna AS, Lehto M, et al. (2011) Endotoxemia is associated with an increased risk of incident diabetes. Diabetes Care 34, 392397.
30 Harte AL, Varma MC, Tripathi G, et al. (2012) High fat intake leads to acute postprandial exposure to circulating endotoxin in type 2 diabetic subjects. Diabetes Care 35, 375382.
31 Mehta NN, McGillicuddy FC, Anderson PD, et al. (2010) Experimental endotoxemia induces adipose inflammation and insulin resistance in humans. Diabetes 59, 172181.
32 Hotamisligil G, Peraldi P, Budavari A, et al. (1996) IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-α and obesity-induced insulin resistance. Science 271, 665668.
33 Brun P, Castagliuolo I, Di Leo V, et al. (2007) Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 292, G518G525.
34 Matsuzaki T, Yamazaki R, Hashimoto S, et al. (1997) Antidiabetic effects of an oral administration of Lactobacillus casei in a non-insulin-dependent diabetes mellitus (NIDDM) model using KK-Ay mice. Endocr J 44, 357365.
35 Matsuzaki T, Nagata Y, Kado S, et al. (1997) Prevention of onset in an insulin-dependent diabetes mellitus model, NOD mice, by oral feeding of Lactobacillus casei . APMIS 105, 643649.
36 Harisa GI, Taha EI, Khalil AF, et al. (2009) Oral administration of Lactobacillus acidophilus restores nitric oxide level in diabetic rats. Aust J Basic Appl Sci 3, 29632969.
37 Tanida M, Imanishi K, Akashi H, et al. (2014) Injection of Lactobacillus casei strain Shirota affects autonomic nerve activities in a tissue-specific manner, and regulates glucose and lipid metabolism in rats. J Diabetes Investig 5, 153161.
38 Triplot NJ, Leber B, Blattl D, et al. (2012) Effect of supplementation with Lactobacillus casei Shirota on insulin sensitivity, β-cell function, and markers of endothelial function and inflammation in subjects with the metabolic syndrome – a pilot study. J Dairy Sci 96, 8995.
39 Wulan SN, Westerterp KR & Plasqui G (2014) Metabolic profile before and after short-term overfeeding with a high-fat diet: a comparison between South Asian and white men. Br J Nutr 111, 18531861.
40 Hudgins LC, Hellerstein M, Seidman C, et al. (1996) Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet. J Clin Invest 97, 20812091.
41 Retzlaff BM, Walden CE, Dowdy AA, et al. (1995) Changes in plasma triacylglycerol concentrations among free-living hyperlipidemic men adopting different carbohydrate intakes over 2 years: the Dietary Alternatives Study. Am J Clin Nutr 62, 988995.
42 Hellerstein MK (2002) Carbohydrate-induced hypertriglyceridemia: modifying factors and implications for cardiovascular risk. Curr Opin Lipidol 13, 3340.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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