Skip to main content
×
×
Home

Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects

  • Carl J. Hulston (a1), Amelia A. Churnside (a1) and Michelle C. Venables (a2)
Abstract

The purpose of the present study was to determine whether probiotic supplementation (Lactobacillus casei Shirota (LcS)) prevents diet-induced insulin resistance in human subjects. A total of seventeen healthy subjects were randomised to either a probiotic (n 8) or a control (n 9) group. The probiotic group consumed a LcS-fermented milk drink twice daily for 4 weeks, whereas the control group received no supplementation. Subjects maintained their normal diet for the first 3 weeks of the study, after which they consumed a high-fat (65 % of energy), high-energy (50 % increase in energy intake) diet for 7 d. Whole-body insulin sensitivity was assessed by an oral glucose tolerance test conducted before and after overfeeding. Body mass increased by 0·6 (se 0·2) kg in the control group (P< 0·05) and by 0·3 (se 0·2) kg in the probiotic group (P>0·05). Fasting plasma glucose concentrations increased following 7 d of overeating (control group: 5·3 (se 0·1) v. 5·6 (se 0·2) mmol/l before and after overfeeding, respectively, P< 0·05), whereas fasting serum insulin concentrations were maintained in both groups. Glucose AUC values increased by 10 % (from 817 (se 45) to 899 (se 39) mmol/l per 120 min, P< 0·05) and whole-body insulin sensitivity decreased by 27 % (from 5·3 (se 1·4) to 3·9 (se 0·9), P< 0·05) in the control group, whereas normal insulin sensitivity was maintained in the probiotic group (4·4 (se 0·8) and 4·5 (se 0·9) before and after overeating, respectively (P>0·05). These results suggest that probiotic supplementation may be useful in the prevention of diet-induced metabolic diseases such as type 2 diabetes.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Probiotic supplementation prevents high-fat, overfeeding-induced insulin resistance in human subjects
      Available formats
      ×
Copyright
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
* Corresponding author: Dr C. J. Hulston, email c.j.hulston@lboro.ac.uk
References
Hide All
1 Tremblay, F, Lavigne, C, Jacques, H, et al. (2001) Defective insulin-induced GLUT4 translocation in skeletal muscle of high fat-fed rats is associated with alterations in both Akt/protein kinase B and atypical protein kinase C activities. Diabetes 50, 19011910.
2 Brons, C, Jensen, CB, Storgaard, H, et al. (2009) Impact of short-term high-fat feeding on glucose and insulin metabolism in young healthy men. J Physiol 587, 22872297.
3 Defronzso, RA (2004) Dysfunctional fat cells, lipotoxicity and type 2 diabetes. Int J Clin Pract 58, 921.
4 Cani, PD, Amar, J, Iglesias, MA, et al. (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56, 17611772.
5 Cani, PD, Bibiloni, R, Knauf, C, et al. (2008) Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57, 14701481.
6 Cani, PD, Possemiers, S, van de Wiele, T, et al. (2009) Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 58, 10911103.
7 Serino, M, Luche, E, Gres, S, et al. (2012) Metabolic adaptations to a high-fat diet is associated with a change in the gut microbiota. Gut 61, 543553.
8 Larsen, N, Vogensen, FK, van den Berg, FWJ, et al. (2010) Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS ONE 5, e9085.
9 Amar, J, Burcelin, R, Ruidavets, JB, et al. (2008) Energy intake is associated with endotoxemia in apparently healthy men. Am J Clin Nutr 87, 12191223.
10 Pendyala, S, Walker, JM & Holt, PR (2012) A high-fat diet is associated with endotoxemia that originates in the gut. Gastroenterology 142, 11001101.
11 Naito, E, Yoshida, Y, Makino, K, et al. (2011) Beneficial effect of oral administration of Lactobacillus casei strain Shirota on insulin resistance in diet-induced obesity mice. J App Microbiol 110, 650657.
12 Ejtahed, HS, Mohtadi-Nia, J, Homayouni-Rad, A, et al. (2012) Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition 28, 539543.
13 Spanhaak, S, Havenaar, R & Schaafsma, G (1998) The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur J Clin Nutr 52, 899907.
14 Matsumoto, K, Takada, T, Shimizu, K, et al. (2010) Effects of a probiotic fermented milk beverage containing Lactobacillus casei strain Shirota on defecation frequency, intestinal microbiota, and the intestinal environment of healthy individuals with soft stools. J Biosci Bioeng 110, 547552.
15 Nagata, S, Asahara, T, Ohta, T, et al. (2011) Effect of the continuous intake of probiotic-fermented milk containing Lactobacillus casei strain Shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged. Br J Nutr 106, 549556.
16 Matsuda, M & DeFronzo, RA (1999) Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care 22, 14621470.
17 Kraegen, EW, Storlien, LH, Jenkins, AB, et al. (1989) Chronic exercise compensates for insulin resistance induced by a high-fat diet in rats. Am J Physiol Endocrinol Metab 256, E242E249.
18 Kim, C, Youg, JH, Park, J, et al. (2000) Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats. Am J Physiol Endocrinol Metab 278, E977E984.
19 Lessard, SJ, Rivas, DA, Chen, K, et al. (2007) Tissue-specific effects of rosiglitazone and exercise in the treatment of lipid-induced insulin resistance. Diabetes 56, 18561864.
20 Todd, MK, Watt, MJ, Le, J, et al. (2007) Thiazolidinediones enhanced skeletal muscle triacylglycerol synthesis while protecting against fatty acid-induced inflammation and insulin resistance. Am J Physiol Endocrinol Metab 292, E485E493.
21 Barclay, JL, Shostak, A, Leliavski, A, et al. (2013) High fat diet-induced hyperinsulinemia and tissue-specific insulin resistance in Cry deficient mice. Am J Physiol Endocrinol Metab 304, E1053E1063.
22 Bielohuby, M, Sisley, S, Sandoval, D, et al. (2013) Impaired glucose tolerance in rats fed low-carbohydrate, high-fat diets. Am J Physiol Endocrinol Metab 305, E1059E1070.
23 Wiedemann, MSF, Wueest, S, Item, F, et al. (2013) Adipose tissue inflammation contributes to short-term high-fat diet-induced hepatic insulin resistance. Am J Physiol Endocrinol Metab 305, E388E395.
24 Adochio, RL, Leitner, JW, Gray, K, et al. (2009) Early responses of insulin signalling to high-carbohydrate and high-fat overfeeding. Nutr Metab 6, 37.
25 Cornford, AS, Hinko, A, Nelson, RK, et al. (2013) Rapid development of systemic insulin resistance with overeating is not accompanied by robust changes in skeletal muscle glucose and lipid metabolism. Appl Physiol Nutr Metab 38, 512519.
26 Yu, C, Chen, Y, Zong, H, et al. (2002) Mechanism by which fatty acids inhibit insulin activation of insulin receptor substrate-1 (IRS-1)-associated phosphatidylinositol 3-kinase activity in muscle. J Biol Chem 277, 5023050236.
27 Kleemann, R, van Erk, M, Verschuren, L, et al. (2010) Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance. PloS ONE 5, e8817.
28 Samuel, VT & Shulman, GI (2012) Mechanisms for insulin resistance: common threads and missing links. Cell 148, 852871.
29 Pussinen, PJ, Havulinna, AS, Lehto, M, et al. (2011) Endotoxemia is associated with an increased risk of incident diabetes. Diabetes Care 34, 392397.
30 Harte, AL, Varma, MC, Tripathi, G, et al. (2012) High fat intake leads to acute postprandial exposure to circulating endotoxin in type 2 diabetic subjects. Diabetes Care 35, 375382.
31 Mehta, NN, McGillicuddy, FC, Anderson, PD, et al. (2010) Experimental endotoxemia induces adipose inflammation and insulin resistance in humans. Diabetes 59, 172181.
32 Hotamisligil, G, Peraldi, P, Budavari, A, et al. (1996) IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-α and obesity-induced insulin resistance. Science 271, 665668.
33 Brun, P, Castagliuolo, I, Di Leo, V, et al. (2007) Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 292, G518G525.
34 Matsuzaki, T, Yamazaki, R, Hashimoto, S, et al. (1997) Antidiabetic effects of an oral administration of Lactobacillus casei in a non-insulin-dependent diabetes mellitus (NIDDM) model using KK-Ay mice. Endocr J 44, 357365.
35 Matsuzaki, T, Nagata, Y, Kado, S, et al. (1997) Prevention of onset in an insulin-dependent diabetes mellitus model, NOD mice, by oral feeding of Lactobacillus casei . APMIS 105, 643649.
36 Harisa, GI, Taha, EI, Khalil, AF, et al. (2009) Oral administration of Lactobacillus acidophilus restores nitric oxide level in diabetic rats. Aust J Basic Appl Sci 3, 29632969.
37 Tanida, M, Imanishi, K, Akashi, H, et al. (2014) Injection of Lactobacillus casei strain Shirota affects autonomic nerve activities in a tissue-specific manner, and regulates glucose and lipid metabolism in rats. J Diabetes Investig 5, 153161.
38 Triplot, NJ, Leber, B, Blattl, D, et al. (2012) Effect of supplementation with Lactobacillus casei Shirota on insulin sensitivity, β-cell function, and markers of endothelial function and inflammation in subjects with the metabolic syndrome – a pilot study. J Dairy Sci 96, 8995.
39 Wulan, SN, Westerterp, KR & Plasqui, G (2014) Metabolic profile before and after short-term overfeeding with a high-fat diet: a comparison between South Asian and white men. Br J Nutr 111, 18531861.
40 Hudgins, LC, Hellerstein, M, Seidman, C, et al. (1996) Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet. J Clin Invest 97, 20812091.
41 Retzlaff, BM, Walden, CE, Dowdy, AA, et al. (1995) Changes in plasma triacylglycerol concentrations among free-living hyperlipidemic men adopting different carbohydrate intakes over 2 years: the Dietary Alternatives Study. Am J Clin Nutr 62, 988995.
42 Hellerstein, MK (2002) Carbohydrate-induced hypertriglyceridemia: modifying factors and implications for cardiovascular risk. Curr Opin Lipidol 13, 3340.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed